• 제목/요약/키워드: plant-growth inhibiting rhizobacterium

검색결과 2건 처리시간 0.016초

Bacillus subtilis IJ-31에서 Hydrocinnamic Acid 생산을 위한 최적배양조건 (Optimization of Culture Condition for the Hydrocinnamic Acid Production from Bacillus subtilis IJ-31)

  • 주길재;김영목;이오석;김정웅;김원찬;송경식;윤성준;김진호;이인구
    • Applied Biological Chemistry
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    • 제48권3호
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    • pp.207-211
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    • 2005
  • 본 연구에서는 식물생장억제미생물 B. subtilis IJ-31이 생산하는 HCA의 최적생산조건을 검토하였다. HCA생산 최적조건으로 탄소원은 1%의 미강, 질소원은 0.5% tryptone, 무기염은 0.1% $ZnCl_2$, 배양 온도는 $37^{\circ}C$, 배양시간은 60시간, pH는 7.0에서 $90.5\;{\mu}g/ml$의 HCA를 생산하였으므로 이를 최적생산조건으로 확립하였다. 이를 바탕으로 통계프로그램(SAS) 중 반응표면 분석법(RSM)을 이용한 jar fermentor 배양에서의 HCA 최적생산 조건을 확립하였다. 즉 토양추출물 2.24% 첨가, 교반속도 290 ppm, tryptone 0.83% 첨가, 배양온도 $37^{\circ}C$, 배양시간 60시간, pH 7.0에서 $102.99\;{\mu}g/ml$의 HCA를 생산하는 최적생산 조건으로 예측되었다. 따라서 실제 상기 RSM 최적 조건에서 실험한 결과 HCA는 $102.5\;{\mu}g/ml$를 생산하였다. 이러한 결과는 플라스크에서 HCA는 $90.5\;{\mu}g/ml$로 생산되었으나 jar fermenter 배양에서는 플라스크 배양에 비하여 12%가량 HCA 생산량이 증가된 결과를 보여주는 것이다.

Inoculation with Bacillus licheniformis MH48 Promotes Nutrient Uptake in Seedlings of the Ornamental Plant Camellia japonica grown in Korean Reclaimed Coastal Lands

  • Park, Hyun-Gyu;Lee, Yong-Seong;Kim, Kil-Yong;Park, Yun-Serk;Park, Ki-Hyung;Han, Tae-Ho;Park, Chong-Min;Ahn, Young Sang
    • 원예과학기술지
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    • 제35권1호
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    • pp.11-20
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    • 2017
  • The objective of this study was to determine whether inoculation with Bacillus licheniformis MH48 as a plant growth-promoting rhizobacterium (PGPR) could promote nutrient uptake of seedlings of the ornamental plant Camellia japonica in the Saemangeum reclaimed coastal land in Korea. B. licheniformis MH48 inoculation increased total nitrogen and phosphorus content in soils by 2.2 and 20.0 fold, respectively, compared to those without bacterial inoculation. In addition, B. licheniformis MH48 produced auxin, which promoted the formation of lateral roots and root hairs, decreased production of growth-inhibiting ethylene, and alleviated salt stress. Total nitrogen and phosphorus uptake of seedlings subjected to bacterial inoculation was 2.3 and 3.6 fold higher, respectively, than the control. However, B. licheniformis MH48 inoculation had no significant effect on the growth of seedlings. Our results suggest that inoculation with B. licheniformis MH48 can be used as a PGPR bio - enhancer to stimulate fine root development, promote nutrient uptake and alleviate salt stress in ornamental plant seedlings grown in the high-salinity conditions of reclaimed coastal land.