• KSBB Journal
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• 제23권4호
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• pp.281-284
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• 2008

본 연구에서는 식물세포배양액에서 회수한 식물세포인 biomass로부터 항암제 paclitaxel을 효율적으로 회수하기 위해, microwave를 이용한 추출 (MAE) 방법을 도입하여 기존의 유기용매 추출 (CSE) 방법과 추출효율을 비교하였다. CSE 방법에서 추출온도 25, $30^{\circ}C$에서는 추출시간 (3, 6, 10 min)에 대해 추출효율이 온도에 영향을 받지 않은 반면, 40, $50^{\circ}C$에서는 추출시간 10 min에서 paclitaxel 수율이 감소하는 경향을 보여 온도에 영향을 받음을 알 수 있었다. CSE 방법의 경우 총 4회의 추출로 식물세포 내 대부분의 paclitaxel을 회수 (>99%) 가능한 반면 MAE 방법의 경우 1회의 추출로 식물세포 내 대부분의 paclitaxel을 회수 (>99%) 가능하였다. MAE 방법이 CSE 방법보다 paclitaxel를 회수하는데 있어서 높은 추출효율, 공정시간 단축, 에너지 절감 효과가 있음을 알 수 있었다. 또한 MAE 공정에서 최적의 biomass/methanol의 비율은 1/1 (w/v)임을 알 수 있었다.

• KSBB Journal
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• 제15권4호
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• pp.346-351
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• 2000

식물세포 배양액으로부터 회수한 식불세포 내 수분 함 량에 따른 추출효율은 건조 정도 보다는 건조방법 에 상당 히 영향을 받음을 알 수 있었으며 건조하여 추출할 경우 사용되는 추출용매를 절약할 수 있었다. 여러가지 유기용 매를 이용하여 paclitaxe1의 추출 경향을 조사한 결과 메탄올의 경우 가장 적 은 양으로 가장 높은 paclitaxe1 회 수율을 얻어 가장 효과적임을 알 수 있었다. 추출방법의 경우 counter-current 형태를 사용할 경우 batch형태에 비하여 용 매 사용량을 줄일 수 있으며 paclitaxe1 회수율은 거의 차이가 없음을 알 수 있었다. Batch 형태를 이용한 메탄올 추출시 식불세포의 경우 4회 (회수율>99%), 식불세포조각의 경우 1회 (회수융>96%)의 추출로 대부분의 paclitaxe1 회수가 가능 하였다. 또한 메탄올 추출시 90% 이상의 머탄올 농도이변 충분하며 (회수율>98%), 추출시biomass와 메탄올 의 흔합비 (Kg biomass: L MeOH)는 1: 1, 추출시간은 1회 5분 이상이면 적당 하였다. 메탄올 추출불에 포함된 극성불 순물들은 다음 공정 인 액/액 (methylene chloride/ MeOH)추 출로 제거하여 정제공정에 사용되어 진다.

• Plant Resources
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• 제6권3호
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• pp.221-226
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• 2003

To improve industrial scale extraction method for extraction of icariin from Epimedium koreanum Nakai, the yields under different extracting conditions such as solvent, temperature, duration and solvent to plant material weight ratio were compared. Regarding extracting solution, highest extracts and icariin yield could be achieved when 10% EtOH was used. In case of plant material to extracting solvent ratio, no significant differences could be observed from 1/10 to 1/50, indicating 1/10 was the most efficient. Extracting temperature significantly affected extracts and icariin yields in that 9$0^{\circ}C$ increased the collected extracts and icariin contents up to 29.6% and 0.76%, respectively, compared to 27.2%, 0.33% at 7$0^{\circ}C$. The yield of extracts was less dependent upon extracting temperature compared to icariin yield. Regarding extraction time, 4 hr and 6 hr resulted in high extracts and icariin yield, respectively. We found extracting Epimedium koreanum Nakai in 10 times volume of 10% EtOH for 4 and 6 hr at 9$0^{\circ}C$ seem to be relatively efficient methods for extracts and icariin, respectively.

• 한국자원식물학회지
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• 제11권1호
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• pp.60-63
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• 1998

For the purposed of improving the utilization of natural chemical pigment, carthamin, of Carthamus tinctorius, the effective extraction methods on this compound were pursued in the present study. The best solvent for the extraction was found to be the 1 %(v/v) NaOH solution, at 25 hours. In addition, more carthamin was extracted flowers from main stem than fromb-ranches . The carthamin content of Korean local safflower was shown to be higher than that of Japanese variety used for medicinal uses.

• 한국환경농학회지
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• 제32권3호
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• pp.171-178
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• 2013

BACKGROUND: Consensus of heavy metal phytoavailability in soils needs to be introduced for soil management protocols in relation to safer food production in the contaminated agricultural soils. For this, setting up the method for evaluation of metal phytoavailability in soil is an essential prerequisite. METHODS AND RESULTS: The current study was carried to select a proper single extraction method for determination of phytoavailable metal concentration in soil. Two extraction methods were examined including 1 M $NH_4NO_3$ extraction and 0.01 M $Ca(NO_3)_2$ extraction methods using 142 soil samples collected from the agricultural soils nearby abandoned mining area in Korea. Corelation analysis was conducted between phytoavailable metal concentrations and soil properties potentially influencing on the metal phytoavailability. Both methods showed similar significance (p<0.001) in correlation with soil properties such as soil pH. However, higher correlation coefficients between phytoavailable metal concentrations and soil properties were observed when used $Ca(NO_3)_2$ extraction rather than using $NH_4NO_3$ extraction. CONCLUSION(S): It appeared that 0.01 M $Ca(NO_3)_2$ extraction was better option for determination of phytoavailable metals in soils and further study to test the efficiency of this method is required in combination with plant uptake.

• 상하수도학회지
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• 제12권3호
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• pp.30-40
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• 1998

Increasing water consumption produced sludge problems of the water treatment plants. The objective of this study is to investigate aluminium coagulants recovery n acidic and alkaline conditions. Water treatment plant sludge produced in Pusan Metropolitan City were tested for the aluminium extraction process. Experiment samples were obtained in summer from water treatment plants of Deoksan and Myongjang. Aluminium coagulants used in these plants during the test period were polyaluminium chloride(PAC), polyaluminium sulfate organic(PSO), polyaluminium sulfate silicate(PASS). Aluminium contents of water treatment sludge were in the range of 7.2~10.9% of the total solids. The recovery percentages for aluminium and iron by acidic extraction method was evaluated to 88% and 42% respectively. Extracted mass variation for other materials such as iron, manganese, total organic carbon was observed during the extraction operation. Alkaline extraction produced more than two times amount of total organic carbon than that in the acidic extraction process.

• 한국약용작물학회지
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• 제19권4호
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• pp.257-263
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• 2011

This study investigated the changes of antioxidant activity and ${\alpha}$-glucosidase inhibitory effect of Jerusalem artichoke (Helianthus tuberosus) 100% methanol extracts by various heat treatment. The contents of total phenolic and flavonoid compounds in methanol extract tended to increased gradually with the rise of temperature to 180$^{\circ}C$. The maximum yield of gallic acid (51.52 ${\pm}$ 2.17mg/g extract weight) and quercetin (13.39 ${\pm}$ 0.03mg/g extract weight) were obtained with extraction temperature of 180$^{\circ}C$ for 120min. In addition, the improving extraction efficiency resulted in the increased biological activities, such as electronic donation ability (EDA, 90.36${\pm}$ 0.57%), reducing power (Abs 1.14) and ${\alpha}$-glucosidase inhibitory effect (92.14 ${\pm}$ 1.14%). Overall, the results of this study indicate that the optimum conditions for the extraction process were an extraction temperature at 180$^{\circ}C$ for 120 min, and will provide the basis for future research on the improving extraction yield of phenolic and flavonoid compounds.

• Journal of Plant Biotechnology
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• 제46권4호
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• pp.338-345
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• 2019

본 연구에서는 개똥쑥 차를 이용한 가공식품 및 천연소재를 개발하고자 물과 다양한 농도의 에탄올로 추출하여 페놀 및 플라보노이드 성분을 분석하였고 항산화 및 항효소 활성을 비교하였다. 개똥쑥 차 추출물은 용매에 따라 총 페놀과 플라보노이드 함량에 유의적인 차이를 보였고 이를 통해 추출요인으로서 에탄올 농도가 중요한 역할을 하는 것으로 확인되었다. 또한 HPLC로 분석된 페놀산과 플라보노이드 정량분석 결과 구성 성분의 차이가 있었으며 이에 따라 항산화 및 항효소 활성에도 영향을 주었다. 개똥쑥 차 이용 측면에서 에탄올 농도 조절을 통해 목적에 맞는 용매 선택을 제안하는 바이다.

• 한국응용곤충학회지
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• 제55권4호
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• pp.377-381
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• 2016

Baermann funnel법을 이용하여 비교적 선명한 선충 분리를 위해서는 Kimwipes 4매 정도를 여과지로 사용하여야 한다. 더욱 효율적이고 경제적인 선충 분리용 여과지를 찾기 위하여 시판되고 있는 15개의 제품을 Kimwipes와 비교 실험을 진행하였다. 선충 분리 효율 검정에는 Heterodera sp.의 유충, Meloidogyne sp.의 유충, Pratylenchus sp., Rhabditis sp., Acrobeloides sp., Panagrolaimus sp., Poikilolaimus sp. 그리고 Diplogasterida 등 8속의 선충을 이용하였다. 전체 선충 분리 효율은 42.0 - 88.8%로 나타났다. 1회 비용, 선충 분리 효율 그리고 선명도를 고려하였을 때, 선명도는 A이고 분리 효율은 69.4%로 Kimwipes 4장과 비슷하며(P=0.05), 비용은 50% 저렴한 국내 업체 모나리자 사의 Pulling Kitchen Towel 1매 사용이 가장 적합하였다.

• 한국식품영양학회지
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• 제29권2호
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• pp.153-161
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• 2016

This study investigated the chemical composition of Solidago virga-aurea var. asiatica Nakai essential oils collected using different extraction methods. The essential oils obtained by simultaneous steam distillation extraction (SDE) and hydrodistillation extraction (HDE) methods from the aerial parts of Solidago virga-aurea var. asiatica Nakai were analyzed by GC and GC-MS. Ninety-nine volatile flavor compounds were identified in the essential oil produced from Solidago virga-aurea var. asiatica Nakai using the SDE method. These compounds were classified into eight categories in terms of chemical functionality: 26 hydrocarbons, 8 aldehydes, 36 alcohols, 7 ketones, 12 esters, 5 oxides and epixides, 4 acids, and a miscellaneous one. Spathulenol (15.66%) was the most abundant compound. Ninety-eight compounds including of 35 hydrocarbons, 6 aldehydes, 29 alcohols, 6 ketones, 10 esters, 4 oxides and epixides, 7 acids, and a miscellaneous one were identified in the essential oil from the plant using the HDE method. Hexadecanoic acid (24.74%) was the most abundant compound. The chemical composition of Solidago virga-aurea var. asiatica Nakai essential oils extracted by SDE and HDE methods are characterized by high content of sesquiterpene alcohols and acids, respectively. The extraction methods may be influenced in the chemical composition of natural plant essential oils.