Korean journal of applied entomology (한국응용곤충학회지)

Korean Society of Applied Entomology (한국응용곤충학회)
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Screening of Tissue Papers for Nematode Extraction for the Baermann Funnel Method

Baermann Funnel법의 선충 분리 여과지 선발

  • Kang, Heonil (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University) ;
  • Eun, Geun (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University) ;
  • Ha, Jihye (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University) ;
  • Lee, Jaehyun (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University) ;
  • Kim, Donggeun (Nematode Research Center, Life and Industry Convergence Research Institute, Pusan National University) ;
  • Kim, Yongchul (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University) ;
  • Choi, Insoo (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University)
  • 강헌일 (부산대학교 식물생명과학과) ;
  • 은근 (부산대학교 식물생명과학과) ;
  • 하지혜 (부산대학교 식물생명과학과) ;
  • 이재현 (부산대학교 식물생명과학과) ;
  • 김동근 (부산대학교 생명산업융합연구원 선충연구센터) ;
  • 김용철 (부산대학교 식물생명과학과) ;
  • 최인수 (부산대학교 식물생명과학과)
  • Received : 2016.06.27
  • Accepted : 2016.10.19
  • Published : 2016.12.01
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Abstract

The Baermann funnel method requires approximately four Kimwipe tissues for research a nematode count under a stereo microscope. To select more efficient and economical nematode extraction paper for nematode extraction, 15 different kinds of tissue papers were tested and compared with Kimwipe tissues. Nematode species used in the extraction efficiency tests include juvenile (J2) of Heterodera sp., J2 of Meloidogyne sp., Pratylenchus sp., Rhabditis sp., Acrobeloides sp., Panagrolaimus sp., Poikilolaimus sp. and Diplogasterida. The extraction efficiency varied between 42.0 to 88.8%. Considering costs, extraction efficacy, and clarity, the Pulling Kitchen Towel (Monalisa Co., Korea) is the best tissue, with clarity A, isolation efficiency of 69.4% (not significantly different compared to Kimwipe 1 ply 88.8%), and 1/2 costs per isolation of Kimwipe 2 ply.

Baermann funnel법을 이용하여 비교적 선명한 선충 분리를 위해서는 Kimwipes 4매 정도를 여과지로 사용하여야 한다. 더욱 효율적이고 경제적인 선충 분리용 여과지를 찾기 위하여 시판되고 있는 15개의 제품을 Kimwipes와 비교 실험을 진행하였다. 선충 분리 효율 검정에는 Heterodera sp.의 유충, Meloidogyne sp.의 유충, Pratylenchus sp., Rhabditis sp., Acrobeloides sp., Panagrolaimus sp., Poikilolaimus sp. 그리고 Diplogasterida 등 8속의 선충을 이용하였다. 전체 선충 분리 효율은 42.0 - 88.8%로 나타났다. 1회 비용, 선충 분리 효율 그리고 선명도를 고려하였을 때, 선명도는 A이고 분리 효율은 69.4%로 Kimwipes 4장과 비슷하며(P=0.05), 비용은 50% 저렴한 국내 업체 모나리자 사의 Pulling Kitchen Towel 1매 사용이 가장 적합하였다.

Keywords

References

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