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http://dx.doi.org/10.5656/KSAE.2016.09.0.035

Screening of Tissue Papers for Nematode Extraction for the Baermann Funnel Method  

Kang, Heonil (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University)
Eun, Geun (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University)
Ha, Jihye (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University)
Lee, Jaehyun (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University)
Kim, Donggeun (Nematode Research Center, Life and Industry Convergence Research Institute, Pusan National University)
Kim, Yongchul (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University)
Choi, Insoo (Department of Plant Bioscience, College of Natural Resource and Science, Pusan National University)
Publication Information
Korean journal of applied entomology / v.55, no.4, 2016 , pp. 377-381 More about this Journal
Abstract
The Baermann funnel method requires approximately four Kimwipe tissues for research a nematode count under a stereo microscope. To select more efficient and economical nematode extraction paper for nematode extraction, 15 different kinds of tissue papers were tested and compared with Kimwipe tissues. Nematode species used in the extraction efficiency tests include juvenile (J2) of Heterodera sp., J2 of Meloidogyne sp., Pratylenchus sp., Rhabditis sp., Acrobeloides sp., Panagrolaimus sp., Poikilolaimus sp. and Diplogasterida. The extraction efficiency varied between 42.0 to 88.8%. Considering costs, extraction efficacy, and clarity, the Pulling Kitchen Towel (Monalisa Co., Korea) is the best tissue, with clarity A, isolation efficiency of 69.4% (not significantly different compared to Kimwipe 1 ply 88.8%), and 1/2 costs per isolation of Kimwipe 2 ply.
Keywords
Baermann funnel method; Filter; Nematode extraction; Soil; Tissue paper;
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Times Cited By KSCI : 1  (Citation Analysis)
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1 de Ruiter, P.C., Neutel, A.M., Moore, J.C., 2005. The balance between productivity and food web structure in soil ecosystems, in: Bardgett, R.D., Usher, M.B., Hopkins, D.W. (Eds.), Biological Diversity and Function in Soil. Cambridge University Press, Cambridge, England, pp. 139-153.
2 Hallmann, J., Viaene, N., 2013. Nematode extraction. Bull. OEPP. 43, 471-495.   DOI
3 Ayoub, S.M., 1977. Plant Nematology: An agricultural training aid. Sacramento: State of California, Dept. Food and Agriculture.
4 Cho, M.R., Choi, Y.E., 1985. Selection of tissue papers for nematode extraction from soil in combined screening-funnel method. Korean J. Plant Prot. 24, 39-44.
5 Kim, D.G., Park, B.Y., Ryu, Y.H., 2012. Soil nematode fauna in Dokdo island of Korea. Res. Plant Dis. 18, 381-386.   DOI
6 McSorley, R., 2004. Soil-Inhabiting Nematodes. The University of Florida. http://entnemdept.ufl.edu/creatures/nematode/soil_nematode.htm.
7 Park, H.C., Kim, S.M., Kim, Y.C., Lee, C.Y., Choi, I.S., 1999. Occurrence of nematodes in carrot fields. J. Agri. Tech. & Dev. Inst. 3, 31-34.
8 SAS. 2014. SAS/STAT Software for PC. Release 9.4 SAS Institute Inc., Cary, NC, USA.
9 Southey, J.F., 1986. Laboratory methods for work with plant and soil nematodes. Her Majesty's Stationery Office, London, UK, pp. 202.
10 Whitehead A.G., Hemming, J.R., 1965. A comparison of some quantitative methods of extracting small vermiform nematodes from soil. Ann. Appl. Biol. 55, 25-38.   DOI
11 Yeates, G.W., 1979. Soil nematodes in terrestrial ecosystems. J. Nematol. 11, 213-229.
12 Yeates, G.W., Bongers, T., De Goede, R.G., Freckman, D.W., Georgieva, S.S., 1993. Feeding habits in soil nematode families and genera-an outline for soil ecologists. J. Nematol. 25, 315-331.
13 Yeates, G.W., Bongers, T., 1999. Nematode diversity in agroecosystems. Agr. Ecosyst. Environ. 74, 133-135.