• Title/Summary/Keyword: pilot-scale culture

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Pilot Study of a Brief WeChat Intervention in China to Increase Students' Willingness to Assist a Flushing Student to Reduce Alcohol Use

  • Zhang, Fan;Yuen, Lok-Wa;Ding, Lanyan;Newman, Ian M.;Shell, Duane F.
    • Journal of Preventive Medicine and Public Health
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    • v.51 no.6
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    • pp.320-325
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    • 2018
  • Objectives: This pilot study tested the effectiveness of a brief alcohol-related intervention delivered by the social media app WeChat to teach about ethanol-induced facial flushing and increase the willingness of students who see another student flushing to suggest that he or she should reduce or stop drinking. In the context of Chinese drinking culture, it is sometimes socially difficult to refuse a drink, even when experiencing physical discomfort, such as flushing. Methods: Classrooms of students in a medical university in China were randomly assigned to the intervention or control group. Students in the intervention group were invited to view 3 alcohol education lessons on WeChat during a 2-week period. A pretest and posttest before and after the 2-week period assessed changes in students' willingness to intervene if they saw someone flush while drinking. Data were collected about students' alcohol use and their ratings of the lessons. Results: Mixed-design analysis of variance yielded a significant time-by-treatment interaction effect on the variable of willingness to suggest that a flushing person stop or slow down their drinking, and the change was significant between the intervention and control groups. One-way analysis of covariance yielded a significant treatment effect at the posttest, after controlling for the pretest score. Students rated the lessons above the midpoint of the scale for being informative, interesting, and useful. Conclusions: The pilot study showed that a brief alcohol-related intervention delivered by WeChat could produce a measurable positive change in the willingness of university students to suggest that a student who flushes should stop drinking. This pilot study also suggested improvements for future lessons and evaluation design.

Development and validation of Everyday Gender Microaggression Scale (일상 속 성차별 경험 척도 개발 및 타당화)

  • Eunha Kim
    • Korean Journal of Culture and Social Issue
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    • v.24 no.4
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    • pp.593-614
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    • 2018
  • By applying the concept of microaggression (subtle and indirect form of discrimination), the aim of this study was to develop and validate the Everyday Gender Microaggression Scale (EGM). For this purpose, based on the results of literature review, surveys, and individual interviews, we developed 17 pilot items. Next, after expert content validation, we administered online survey to 180 adult women and conducted exploratory factor analysis. The result revealed 2 factors, 14 items, and this structure was reconfirmed through confirmatory factor analysis in another sample of 219 adult women. Convergent and concurrent validities were also examined via correlations with measures of sexism, depression, and life satisfaction. We discussed implications, ways to use the EGM, and suggestions for future research.

Strain Improvement and Bioprocess Optimization for Enhanced Production of Haluronic Acid(HA) in Bioreactor Cultures of Streptococcus zooepidemicus (히알루론산 생산성 향상을 위한 Streptococcus zooepidemicus 균주 개량 및 발효조 배양공정 최적화)

  • Kim, Soo Yeon;Chun, Gie-Taek
    • Microbiology and Biotechnology Letters
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    • v.48 no.3
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    • pp.344-357
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    • 2020
  • Strain improvement and bioprocess development were undertaken to enhance hyaluronic acid(HA) production by Streptococcus zooepidemicus cells. Using a high-yielding mutant strain, statistical medium optimization was carried out in shake flask cultures, resulting in 52% increase in HA production (5.38 g/l) at the optimal medium composition relative to the parallel control cultures. For sufficient supply of dissolved oxygen (DO), which turned out to be crucial for enhanced production of HA, agitation system and speed were intensively investigated in 5 L bioreactor cultures. Increase in oxygen mass transfer coefficient (kLa) through increment of agitation speed (rpm) and 35% expansion of diameter of the newly-designed impellers showed significantly positive effects on HA production. By installing an expanded Rushton-turbine impeller for efficient break-down of sparged air, and an extended marine impeller above the Rushton-turbine impeller for efficient mixing of the air-born viscous fermentation broth, maximum amount of HA (9.79 g/l) was obtained at 450 rpm, 1.8 times higher level than that of the corresponding flask culture. Subsequently, the possibility of bioprocess scale-up to a 50 L bioreactor was investigated. Despite almost identical maximum HA production (9.11 vs 9.25 g/l), the average HA volumetric productivity (rp) of the 50 L culture turned out only 74% compared to the corresponding 5 L culture during the exponential phase, possibly caused by shear damages imposed on the producing cells at the high stirring in the 50 L culture. The scale-up process could be successfully achieved if a scale-up criterion of constant oxygen mass transfer coefficient (kLa) is applied to the 50 L pilot-scale bioreactor system.

Development of the Patient Safety Nursing Activities Scale for Clinical Nurses (임상간호사의 환자안전 간호활동 측정도구 개발)

  • Kim, Hwa-Young;Ryu, Seang
    • Journal of Digital Convergence
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    • v.16 no.9
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    • pp.207-217
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    • 2018
  • The purpose of this study was to develop a patient safety nursing activities scale for clinical nurses and to verify validity and reliability it. A preliminary scale was developed through various processes and pilot study. Data were collected from 900 nurses at 28 hospitals selected by proportionate stratified sampling with 331 general hospitals nationwide. The final scale was a 4-point scale, consisting of 72 items in 10 domains. The construct validity was found to be appropriate by exploratory factor analysis and 70.3% of the variance in the total scale and over .74 of factor loading. There was a significant correlation between scale and patient safety culture (r=.52, p<.001) and Cronbach's ${\alpha}$ was .99. This scale developed to measure patient safety nursing activities of clinical nurses was found to be reliable and valid and will be a useful tool in practical field.

Study on the Ways to Improve Cultural Impact Assessment System (문화영향평가제도 개선 방안에 관한 연구)

  • Kwon, Ki-Chang
    • The Journal of the Korea Contents Association
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    • v.16 no.1
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    • pp.415-423
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    • 2016
  • Cultural impact assessment system is a program to improve the quality of people's life by minimizing the negative effects and maximizing the positive effects on the cultural environment when a central government or local governments implement policies or plans. It was introduced in 2013 by Framework Act on the Promotion of Cultural Industries. Now the Ministry of Culture, Sports and Tourism is promoting a pilot project for cultural impact assessment system to reduce trial and error and settle it as a successful system. This study examined previous studies, international case studies, and various cultural impact assessment systems in oder to successfully seated cultural impact assessment systems and proposed ways to improve evaluations regarding objects, index, process, scale of objects, and evaluation agency. For the system to run smoothly in the future, additional researches are needed on the scale of evaluation objects, setting up detailed evaluation index, the composition of evaluation committee and its operation, and the introduction of cultural impact evaluators.

A Study on the Types of Interior Design Image and Consisting Elements in the Department Store -focused on the first floor of Department Store- (국내 백화점 실내디자인 이미지 유형 및 구성요소 분석 연구-수도권지역 대형 백화점 1층 공간을 중심으로-)

  • 정수현;최상헌
    • Korean Institute of Interior Design Journal
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    • no.22
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    • pp.46-52
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    • 2000
  • The Department store of today contrary to past plays an important role in the culture of modern market, because of the development of national economy and a rise in the standard of living. The improvement of culture and the high growth of economy made a livelihood fertile, mass production and mass consumption of product based on accumulation of technology caused the industry of distribution to activate, and the change of consumption pattern and various purchasing form resulted in the distribution structure like large scale store. This study was composed of pilot study, the first research, and the second research. In pilot study, the types of interior design the first floor of large-sized department store in the Metropolitan area was classified on the basis of the image of interior design prior researched. The contemporary interior design require unique image frequently because of the qualitative enhancement accompanying segmentation and specialization of space. Especially, the emergence of foreign company along with the change of the distribution environment threaten the domestic interior design industry. Therefore, the interior design plant introducing the image elements is able to have critical influence on forming specialized space in department store. This study investigated the interior design limited to the first floor of large-sized department store in the Metropolitan area, therefore the user's preference and the problem of design in domestic department store of today was not examined well. But this study was able to explore the types of interior design image in the first floor of department store and the relation between consisting elements.

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Development and Validation of Adaptive Game Use Scale (AGUS) (적응적 게임활용 척도 개발 및 타당화)

  • Hoon-Seok Choi ;Kyo-Heon Kim ;Joung Soon Ryong ;Keum-Mi Kim
    • Korean Journal of Culture and Social Issue
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    • v.15 no.4
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    • pp.565-589
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    • 2009
  • The present study explored the major components of adaptive game behavior among adolescents in Korea. Based on relevant research and a pilot testing, an Adaptive Game Use Scale (AGUS) was developed and validated. A stratified sampling procedure was used to draw a representative sample, and a total of 600 male and female students from middle schools and high schools in various regions participated in the study. Factor analyses revealed 7 facets of adaptive game behavior, including experiencing vitality, expanding life experience, making good use of leisure time, experiencing flow, exercising control, experiencing self-esteem, maintaining and expanding social network. Internal consistency and temporal stability(4 weeks) of the scale were both high. A confirmatory factor analysis indicated that a 7-factor hierarchical model fits well with the data. Moreover, additional analyses suggested that AGUS and game addiction are conceptually distinct. Correlational analyses also indicated that AGUS has good discriminant validity and concurrent validity. Implications of the findings and future directions were discussed.

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Removal of Gaseous Styrene using a Pilot-Scale Rotating Drum Biotrickling Filter (Pilot-scale 회전식 드럼 바이오필터를 이용한 Styrene 제거)

  • Hwang, Jae-Woong;Lim, Ji-Sung;Chang, Seok-Jin;Lee, Eun-Yul;Choi, Cha-Yong;Park, Sung-Hoon
    • KSBB Journal
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    • v.21 no.3
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    • pp.188-193
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    • 2006
  • A new type of biofilter, a rotating drum biotrickling filter(RDBF), was developed and operated for the removal of styrene from industrial waste gas. The porous polyurethane foam sheet was used as a packing materials for the RDBF and a pure culture of Gram-positive bacterium Brevibacillus sp. SP1 was used as an inoculum. The reactor showed a short start-up period of 18 days, during which uniform biofilms were developed on the packing. During a steady operation at an incoming styrene concentration of $200ppm_v$ and a retention time of 0.5 min, a high and stable removal of styrene over 95% was observed. The maximum elimination capacity was estimated to be $125g/m^3{\cdot}hr$. The outstanding performance was attributed to an efficient gas-liquid mass transfer and the appropriate supply of nutrient solution to the biofilm microorganisms on the packing by the rotation of the drum.

Characteristics of Algae Occurrence on Environmental Changes (환경변화에 따른 조류 발생 변화)

  • Noh, Seongyu;Shin, Yuna;Choi, Heelak;Lee, Jaeyoon;Lee, Jaean;Rhew, Doughee
    • Journal of Environmental Impact Assessment
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    • v.24 no.3
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    • pp.278-286
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    • 2015
  • Pilot scale system was designed to identify the growth and movement of algae, depending on environmental changes(retention time, nutrient concentration, etc) in Gangjeong-Goryeong Weir of the Nakdong River. Considering the stability of algal culture and easy observation of algal growth, pilot scale system was made of transparent acrylic material(3 sets of flexible cylindrical water tanks with 1 m diameter and 4 m height). Auxiliary equipments include light intercepter, water inflow device for different water depth and storage of reclaimed water. The retention time was 2 days(before construction of weir; treatment 1), 8 days(after construction of weir, 2013; treatment 2) and 30 days(2014; treatment 3). According to the water temperature of treatment 1 were similar by depth, treatment 3 showed a difference between the surface(0 m) and bottom(4 m) more than $3^{\circ}C$. DO, pH showed relatively high in the surface than the bottom. Nutrients showed eutrophic condition in all experiments. The Chlrophyll-a concentration of the treatment 1 showed a relatively lower value than the Chlrophyll-a concentration of the treatment 2 and 3. Therefore, the retention time was considered to influence the growth of phytoplankton.

Effect of Carbon Source Consumption Rate on Lincomycin Production from Streptomyces lincolnensis

  • Choi, Du-Bok;Cho, Ki-An
    • Journal of Microbiology and Biotechnology
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    • v.14 no.3
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    • pp.532-539
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    • 2004
  • For efficient lincomycin production from Streptomyces lincolnensis L1245, various vegetable oils, natural nitrogen sources, and surfactants were investigated at the pilot-scale level in the flask. Olive oil as the sole carbon source was the most suitable one for producing lincomycin. When 20 g/lof olive oil was used, the lincomycin concentration and lipase activity reached 1.01 g/land 182 U/ml, respectively, after 5 days of culture. Among the various unsaturated fatty acids, when linolenic acid was used, the cell growth and lincomycin production were markedly decreased. On the other hand, when 0.2 g/l of oleic acid was added to the culture broth, the maximum lincomycin concentration was 1.0 g/l, which was about 1.7-fold higher than that obtained without the addition of oleic acid. Among the various natural nitrogen sources, pharmamedia or soybean meal was the most suitable nitrogen source. In particular, in the case of a mixture of 10 g/l of pharmamedia and soybean meal, 1.5 g/l of lincomycin concentration and 220 U/ml of lipase activity were obtained. When Span 180 was used as the surfactant, lincomycin production, lipase activity, and oil consumption increased. The correlation between the consumption rates of oil and lincomycin production in a culture using olive oil as the sole carbon source was also investigated. The lincomycin production depended on the consumption rate of olive oil. Using these results, fed-batch cultures for comparing the use of olive oil and starch as a conventional carbon source were carried out in a 5-1 fermentor. When olive oil was used as the sole carbon source, 34 g/l of olive oil was consumed after 7 days of culture. The maximum lincomycin concentration was 3.0 g/l, which was about 2.0-fold higher than that of starch medium after 7 days of culture. The product yield was 0.09 gig of consumed carbon source, which was about 3.0-fold higher than that of starch medium after 7 days of culture.