• Korean Journal of Fisheries and Aquatic Sciences
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• v.29 no.6
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• pp.849-855
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• 1996

Processing condition and pigment maintenance of cockle, Fulvia mutica were studied. Proximate composition of whole meat was $82.3\%$ moisture, $10.8\%$ crude protein, $0.8\%$ crude lipid, $2.5\%$ carbohydrate and $3.1\%$ crude ash, and that of foot muscle was $80.6\%,\;12.3\%,\;0.3\%,\;2.9\%\;and\;3.3\%$ respectively. When the living cockle was soaked in $2\%$ NaCl solutions, about $90\%$ of silt and mud was removed after 10 hours soaking, and over $92\%$ was removed when the pH was adjusted to 7.5. When the pigment destruction was tested by 40 seconds at $75^{\circ}C,\;80^{\circ}C,\;85^{\circ}C,\;90^{\circ}C\;and\;95^{\circ}C$, retention ratios of pigment in cockle were above $95\%$ at all temperature. Soaking in ethanol for 5 minutes resulted in strong adhesion of pigment to meat. Soaking in seasoning liquid containing $10\%$ soy sauce, $5\%$ wasabi, $5\%$ sugar, $2\%$ vinegar, $2\%$ powdered garlic for 3 minutes was effective for instant processing of cooked and frozen cockle after thawing. After 60 days storage at $-20^{\circ}C$, the contents of moisture, crude lipid, carbohydrate, ash and salinity were not changed so much, and pH and TBA values increased to 6.6 and 0.3 compared with 6.2 and 0.2, respectively, while pigment absorbance at 226 nm was decreased from 2.7 to 2.3. However, in case of 60 days storage at $-45^{\circ}C$, there was no change in these compositions.

• Molecules and Cells
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• v.44 no.3
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• pp.168-178
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• 2021

The retinal pigment epithelium (RPE) forms a monolayer sheet separating the retina and choroid in vertebrate eyes. The polarized nature of RPE is maintained by distributing membrane proteins differentially along apico-basal axis. We found the distributions of these proteins differ in embryonic, post-natal, and mature mouse RPE, suggesting developmental regulation of protein trafficking. Thus, we deleted tumor susceptibility gene 101 (Tsg101), a key component of endosomal sorting complexes required for transport (ESCRT), in embryonic and mature RPE to determine whether ESCRT-mediated endocytic protein trafficking correlated with the establishment and maintenance of RPE polarity. Loss of Tsg101 severely disturbed the polarity of RPE, which forms irregular aggregates exhibiting non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling. These findings suggest that ESCRT-mediated protein trafficking is essential for the development and maintenance of RPE cell polarity.

• Molecules and Cells
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• v.46 no.7
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• pp.441-450
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• 2023

β-Catenin (Ctnnb1) has been shown to play critical roles in the development and maintenance of epithelial cells, including the retinal pigment epithelium (RPE). Ctnnb1 is not only a component of intercellular junctions in the epithelium, it also functions as a transcriptional regulator in the Wnt signaling pathway. To identify which of its functional modalities is critically involved in mouse RPE development and maintenance, we varied Ctnnb1 gene content and activity in mouse RPE lineage cells and tested their impacts on mouse eye development. We found that a Ctnnb1 double mutant (Ctnnb1^dm), which exhibits impaired transcriptional activity, could not replace Ctnnb1 in the RPE, whereas Ctnnb1^Y654E, which has reduced affinity for the junctions, could do so. Expression of the constitutively active Ctnnb1^∆ex3 mutant also suppressed the development of RPE, instead facilitating a ciliary cell fate. However, the post-mitotic or mature RPE was insensitive to the loss, inactivation, or constitutive activation of Ctnnb1. Collectively, our results suggest that Ctnnb1 should be maintained within an optimal range to specify RPE through transcriptional regulation of Wnt target genes in the optic neuroepithelium.

• The Journal of Information Technology
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• v.7 no.1
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• pp.105-110
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• 2004

In this study, acrylic water-soluble coatings were prepared by the white pigment($TiO_2$) and acrylic resin. The basic properties of acrylic water-soluble coatings were measured, and the maintenance of perfume was investigated by FT-IR spectrophotometer and sensory test.

• BMB Reports
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• v.56 no.2
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• pp.84-89
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• 2023

The implications of nutrient starvation due to aging on the degeneration of the retinal pigment epithelium (RPE) is yet to be fully explored. We examined the involvement of AMPK activation in mitochondrial homeostasis and its relationship with the maintenance of a healthy mitochondrial population and epithelial characteristics of RPE cells under nutrient starvation. Nutrient starvation induced mitochondrial senescence, which led to the accumulation of reactive oxygen species (ROS) in RPE cells. As nutrient starvation persisted, RPE cells underwent pathological epithelial-mesenchymal transition (EMT) via the upregulation of TWIST1, a transcription regulator which is activated by ROS-induced NF-κB signaling. Enhanced activation of AMPK with metformin decelerated mitochondrial senescence and EMT progression through mitochondrial biogenesis, primed by activation of PGC1-α. Thus, by facilitating mitochondrial biogenesis, AMPK protects RPE cells from the loss of epithelial integrity due to the accumulation of ROS in senescent mitochondria under nutrient starvation.

• Polymer(Korea)
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• v.38 no.1
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• pp.24-30
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• 2014

Retinal pigment epithelium (RPE) plays an important role in maintaining the visual function and the degeneration of the RPE causes several retinal degeneration disease. In order to fabricate the suitable carrier for RPE transplantation, the hybrid poly(lactide-co-glycolide) (PLGA) film with hesperidin was prepared. Hesperidin has an anti-inflammatory and antioxidant characteristics. ARPE-19 was seeded on hesperidin/PLGA film and then, cell proliferation was determined by the MTT assay, and cell adhesion and cell morphology were confirmed by SEM. Also, RT-PCR was performed to confirm the expression of the specific genes, and AEC immunohistochemical staining was performed to determine the expression of RPE65. As a result, we confirmed that attachment, proliferation and phenotype maintenance of RPE cells were more excellent on hesperidin/PLGA film than PLGA film, thereby we were able to confirm the potential applications of hesperidin/PLGA film as tissue engineering carrier for regeneration of retina.

• Journal of the Society of Cosmetic Scientists of Korea
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• v.25 no.4 s.34
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• pp.7-16
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• 1999

Silver-containing antimicrobial inorganic pigments that have been developed so far still have problems, which result from silver's unique metallic color and discoloration. Therefore, those things are used only for make-up cosmetics or just the restricted amount is used. Although the use of white-base pigments or iron oxides has been considered to solve those problems, they virtually fail to serve as a perfect substitute. So it seems difficult to use enough quantity of those materials or to apply them to diverse kinds of products. The purpose of this study was, accordingly, to attain the complete removal of metallic color and the maintenance of color. Additionally, a rosemary extract was employed to develop a silver-containing inorganic antimicrobial pigment(Ag-AIP-R) that has an improved antimicrobial effect and anti oxidative effect.

• The Journal of Information Technology
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• v.5 no.4
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• pp.139-144
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• 2002

In this study, acrylic water-soluble coatings were prepared by the white pigment($TiO_2$) and acrylic resin. The basic properties of acrylic water-soluble coatings were measured, and the maintenance of perfume(lemon) was investigated by FT-IR spectrophotometer and sensory test.

• The Journal of Information Technology
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• v.5 no.2
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• pp.11-16
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• 2002

In this study, acrylic water-soluble coatings were prepared by the white pigment($TiO_2$) and acrylic resin. The basic properties of acrylic water-soluble coatings were measured, and the maintenance of perfume was investigated by FT-IR spectrophotometer and sensory test.

• Proceedings of the SCSK Conference
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• 1999.10a
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• pp.7-16
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• 1999

Silver-containing antimicrobial inorganic pigments that have been developed so far still have problems, which result from silver’s unique metallic color and discoloration. Therefore, those things are used only far make-up cosmetics or just the restricted amount is used. Although the use of white-base pigments or iron oxides has been considered to solve those problems, they virtually fail to serve as a perfect substitute. So it seems difficult to use enough quantity of those materials or to apply them to diverse kinds of products. The purpose of this study was, accordingly, to attain the complete removal of metallic color and the maintenance of color. Additionally, a rosemary extract was employed to develop a silver-containing inorganic antimicrobial pigment(Ag-AIP-R) that has an improved antimicrobial effect and antioxidative effect.