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http://dx.doi.org/10.14348/molcells.2021.0027

Tsg101 Is Necessary for the Establishment and Maintenance of Mouse Retinal Pigment Epithelial Cell Polarity  

Le, Dai (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Lim, Soyeon (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Min, Kwang Wook (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Park, Joon Woo (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Kim, Youjoung (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Ha, Taejeong (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Moon, Kyeong Hwan (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Wagner, Kay-Uwe (Department of Oncology, Wayne State University)
Kim, Jin Woo (Department of Biological Sciences, Korea Advanced Institute of Science and Technology (KAIST))
Publication Information
Molecules and Cells / v.44, no.3, 2021 , pp. 168-178 More about this Journal
Abstract
The retinal pigment epithelium (RPE) forms a monolayer sheet separating the retina and choroid in vertebrate eyes. The polarized nature of RPE is maintained by distributing membrane proteins differentially along apico-basal axis. We found the distributions of these proteins differ in embryonic, post-natal, and mature mouse RPE, suggesting developmental regulation of protein trafficking. Thus, we deleted tumor susceptibility gene 101 (Tsg101), a key component of endosomal sorting complexes required for transport (ESCRT), in embryonic and mature RPE to determine whether ESCRT-mediated endocytic protein trafficking correlated with the establishment and maintenance of RPE polarity. Loss of Tsg101 severely disturbed the polarity of RPE, which forms irregular aggregates exhibiting non-polarized distribution of cell adhesion proteins and activation of epidermal growth factor receptor signaling. These findings suggest that ESCRT-mediated protein trafficking is essential for the development and maintenance of RPE cell polarity.
Keywords
cell polarity; endosomal sorting complexes required for transport (ESCRT); retinal pigment epithelium (RPE); tumor susceptibility gene 101 (Tsg101);
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