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http://dx.doi.org/10.7317/pk.2014.38.1.24

Adhesion and Proliferation Behavior of Retinal Pigment Epithelial Cells on Hesperidin/PLGA Films  

Lee, So Jin (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National University)
Kang, Su Ji (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National University)
Kim, Hye Yun (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National University)
Lee, Jung Hwan (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National University)
Kim, Eun Young (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National University)
Kwon, Soon Yong (Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea)
Chung, Jin Wha (Dept. of Orthopedic Surgery, Yeouido St. Mary's Hospital, Catholic University of Korea)
Joo, Choun-Ki (Dept. of Ophthalmology and Visual Science, Seoul St. Mary's Hospital, College of Medicine, Catholic University of Korea)
Khang, Gilson (Dept. of BIN Fusion Tech., Polymer Fusion Res. Center & Dept. of PolymerNano Sci. Tech., Chonbuk National University)
Publication Information
Polymer(Korea) / v.38, no.1, 2014 , pp. 24-30 More about this Journal
Abstract
Retinal pigment epithelium (RPE) plays an important role in maintaining the visual function and the degeneration of the RPE causes several retinal degeneration disease. In order to fabricate the suitable carrier for RPE transplantation, the hybrid poly(lactide-co-glycolide) (PLGA) film with hesperidin was prepared. Hesperidin has an anti-inflammatory and antioxidant characteristics. ARPE-19 was seeded on hesperidin/PLGA film and then, cell proliferation was determined by the MTT assay, and cell adhesion and cell morphology were confirmed by SEM. Also, RT-PCR was performed to confirm the expression of the specific genes, and AEC immunohistochemical staining was performed to determine the expression of RPE65. As a result, we confirmed that attachment, proliferation and phenotype maintenance of RPE cells were more excellent on hesperidin/PLGA film than PLGA film, thereby we were able to confirm the potential applications of hesperidin/PLGA film as tissue engineering carrier for regeneration of retina.
Keywords
retinal pigment epithelial cells; hesperidin; PLGA; hesperidin/PLGA hybrid film;
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