• Title/Summary/Keyword: phytotoxicity

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Cadmium-Induced Phytotoxicity in Tomato Seedlings Due to the Accumulation of H2O2 That Results from the Reduced Activities of H2O2 Detoxifying Enzymes

  • Cho, Un-Haing
    • The Korean Journal of Ecology
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    • v.27 no.2
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    • pp.107-114
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    • 2004
  • Tomato (Lycopersicon esculentum) seedlings exposed to various concentrations of $CdC1_2$ (0∼100 $\mu$M) in the nutrient solution for up to 9 days were analyzed with the seedling growth, $H_2O_2$ production, glutathione levels and activity changes of enzymes related to $H_2O_2$ removal. The growth of seedlings was inhibited with over 50 $\mu$M Cd, whereas the levels of $H_2O_2$ and glutathione were enhanced with Cd exposure level and time. Meanwhile, Cd exposure increased the activities of catalase (CAT) and glutathione reductase (GR) but decreased the activities of dehydroascorbate acid reductase (DHAR) and ascorbate peroxidase (APX) in both leaves and roots. These results suggest that the altered activities of antioxidant enzymes particularly involved in the $H_2O_2$ removal and the subsequent $H_2O$$_2$ accumulation could induce the Cd-induced phytotoxicity.

Production of a Phytotoxic Substance by Exserohilum monoceras, the Causal Fungus of Barnyardgrass Leaf Blight, and its Response on Host Plants (논피잎마름병균(Exserohilum monoceras)의 독소생산과 그 기주반응)

  • 조재민;홍연규;엄재열
    • Korean Journal Plant Pathology
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    • v.13 no.2
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    • pp.132-137
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    • 1997
  • Phytotoxicity of the culture filtrate and culture conditions for the production of the phytotoxin by Exserohilum monoceras 92-044 were examined. The necrotic lesions were developed on the leaves of Echinochloa crus-galli within 48 hrs after inoculation of the culture filtrate, and the leaves were completely blighted within 5∼7 days. Maximum toxicity was found in the culture broth containing 20% V-8 juice. Phytotoxin accumulation and fungal growth reached their highest peak at around 11 days. Typical symptom appeared on the leaf of E. crus-galli within 48 hrs. Only a weak chlorosis appeared on rice, Arundinella hirta (THUNB) and henry crabgrass (Digitaria sanguinalis SCOP.), but no further symptom developed.

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Purification and Phytotoxicity of Apicidins Produced by the Fusarium semitectum KCTC16676

  • Jin, Jianming;Baek, Seung-Ryel;Lee, Kyung-Rim;Lee, Jungkwan;Yun, Sung-Hwan;Kang, Seog-Chan;Lee, Yin-Won
    • The Plant Pathology Journal
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    • v.24 no.4
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    • pp.417-422
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    • 2008
  • Apicidin is a cyclic tetrapeptide produced by some Fusarium species and is known to inhibit Apicomplexan histone deacetylase. The goals of this study were to determine species identity of Fusarium isolate KCTC16676, an apicidin producer, to improve a method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which showed that KCTC16676 belongs to the F. semitectum-F. equiseti species complex. To enhance apicidin production, after culturing isolate KCTC16676 on a wheat medium for 3 weeks at $25^{\circ}C$, the culture was extracted with chloroform. Apicidins were purified through a reverse phase $C_{18}$ silica gel column, resulting in 5 g of apicidin, 200 mg of apicidin A, and 300 mg of apicidin $D_2$ from 4 kg of wheat cultures; this represents a significant yield improvement from a previous method, offers more materials to study the modes of its action, and facilitates the elucidation of the apicidin biosynthesis pathway. Apicidin and apicidin $D_2$ showed phytotoxicity on both seedlings and 2-week-old plants of diverse species, and weeds were more sensitive to apicidins than vegetables

Characterization and Phytotoxicity of Zn, Zn Oxide Nanoparticles (아연 나노 입자와 산화아연 나노 입자의 특성과 식물독성)

  • Kim, Sung-Hyun;Baek, Ju-Hyung;Song, Yi-Reh;Sin, Min-Joo;Lee, In-Sook
    • Journal of Korean Society of Environmental Engineers
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    • v.31 no.12
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    • pp.1129-1134
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    • 2009
  • Increasing application of nanotechnology highlights the need to clarify nanotoxity and nanoparticles characterization. However, few researches have focused on phytotoxicity of nanoparticles. This study was to examine phytotoxicity on Cumumis sativus seedling and the dissolution of Zn, ZnO nanoparticles in hydroponic culture system. Results of this study; characteristics of Zn, ZnO nanoparticles are more aggregated in nutrient solution than deionized water. C. sativus biomass significantly reduced in the nutrient solution were higher than 100 mg/L, and Zn toxicity showed $Zn^{2+}$> Zn> ZnO NPs. Results of transmission electron microscopy images, Zn and ZnO nanoparticles greatly adhered onto the root cell wall and nanoparticles were observed in the root cell.

Effect of Aging on the Chemical Forms and Phytotoxicity of Arsenic in Soil (비소 오염기간이 토양 내 비소의 존재형태와 식물독성에 미치는 영향)

  • Yang, Woojin;Jho, Eun Hea;Im, Jinwoo;Jeong, Seulki;Nam, Kyoungphile
    • Journal of Soil and Groundwater Environment
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    • v.21 no.3
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    • pp.82-87
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    • 2016
  • This study investigates effects of an aging period on arsenic (As) chemical forms in soils and phytotoxicity using artificially As-contaminated soils with a range of As concentrations (0-300 mg/kg) and aging periods (0 and 3 months). A sequential extraction procedure showed that the increasing As concentration in soils increased the ratio of non-specifically and specifically bound As, which are known to be bioavailable. This resulted in increasing As uptake by tomatoes with increasing As concentration (R2=0.87 for exponential fitting); however, the seed germination was not sensitive to the As concentrations of the soil samples. The seed germination was also statistically similar in the soils with 75 and 150 mg-As/kg regardless of the aging period. The time taken until the seed germination (i.e., lag phase), on the other hand, decreased from 10 d to 3 d with aging for 3 months. This can be attributed to the decreased amount of bioavailable As with aging. Overall, this study shows that when the toxic effects of the As-contaminated soils are assessed using tomato plants, it is better to use more sensitive methods than seed germination such as the As accumulation or the lag phase for seed germination.

Influence of Foliar Application of Mixed Solution of Urea-Chemicals on Rice Plant Growth (비료농약(肥料農藥) 혼용살포(混用撒布)가 수도생육(水稻生育)에 미치는 영향(影響))

  • Shin, Jae-Sung;Seong, Ki-Seog;Oh, Byung-Youl
    • Korean Journal of Soil Science and Fertilizer
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    • v.19 no.1
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    • pp.21-24
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    • 1986
  • This experiment was conducted to find out the feasibility of foliar application of mixed solution of urea and agricultural chemicals. One to two percent urea solution and chemicals were combined for joint application and the degradation of mixed solution was tested and its phytotoxicity was evaluated on rice plant. There were no changes of pH and components of mixed solution of urea and chemicals. However, it led to phytotoxicity in rice plant and more severe in higher urea content. The yields of rice were slightly lower in the mixed solution plot than in check one, however, no significant statistical difference was observed. Due to phytotoxicity, the foliar application of mixed solution is not agronomically sound.

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Herbicidal Phytotoxicity of Early Rice Seedlings as Affected by Cultural Practices IV. Response of Phytotoxicity with Application Rate of Fertilizer and Organic Matter (재배조건(栽培條件)의 차이(差異)가 수도(水稻)어린묘(苗의) 제초제(除草劑) 약해발생(藥害發生)에 미치는 영향(影響) IV. 시비량(施肥量) 및 유기물(有機物) 시용량(施用量)에 따른 약해반응(藥害反應))

  • Han, S.U.;Guh, J.O.;Kwon, S.L.
    • Korean Journal of Weed Science
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    • v.11 no.2
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    • pp.106-110
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    • 1991
  • There was a tendency that the growth inhibition of early growth due to herbicides was increased with increasing the amount of fertilizers. As a matter of fact, pretilachlor and pyrazolate increased the degree of injury with increasing the amount of fertilizers, and dimepiperate was actually safe regardless of the amount of fertilizer applied, while in case of bensulfuron, the injury become less when it was applied without fertilizers. As for organic matters, dimepiperate and pyrazolate were rather safe without organic matter, while pretilachlor and bensulfuron become really safe with the application of organic matter more than 1,000 kg/10a.

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Herbicidal Phytotoxicity of Early Rice Seedlings as Affected by Cultural Practices III. Response of Phytotoxicity with Water Depth and Drainage Level per Day (재배조건(栽培條件)의 차이(差異)가 수도(水稻)어린묘(苗의) 제초제(除草劑) 약해발생(藥害發生)에 미치는 영향(影響) III. 담수심(湛水深) 및 감수심(減水深) 차이(差異)에 따른 약해반응(藥害反應))

  • Han, S.U.;Guh, J.O.;Kwon, S.L.
    • Korean Journal of Weed Science
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    • v.11 no.2
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    • pp.100-105
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    • 1991
  • Maintaining the water level at 5cm depth showed less decreasing in number of tiller and dry weight as compared to 1 and 3cm depths when Bensulfuron and Pretilachlor were applied. The drainage more than 5cm as water level everyday caused the growth inhibition when Bensulfuron and Pretilachlor were used, while Dimepiperate was rather safe.

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Studies on the Selectivity of Herbicide Alachlor;I. Phytotoxicity and Glutathione Conjugation (제초제 Alachlor의 선택성에 관한 연구;I. 약해와 글루타치온 Conjugation 반응)

  • Park, Chang-Kyu;Hwang, Eul-Chul
    • Korean Journal of Environmental Agriculture
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    • v.6 no.1
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    • pp.44-49
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    • 1987
  • Present work has been initiated to see if inherent biochemical difference among plants is, in any way, related to the observed selectivity characteristics of preemergence herbicide, alachlor. Application of aqueous solution of alachlor onto three intact plants, soybean, chinese cabbage and barnyard grass resulted in phytotoxicity responses in the testt plants in varying degree. Examination of glutathione (and homoglutathione) contents of the test plants indicated that the phytotoxicity is inversely proportional to the peptide contents of the test plants. It was also noted that four to five water soluble metabolites are readily formed in intact seedling treated with labelled alachlor and glutathionealachlor and homoglutathionealachlor conjugates were tentatatively identified as major metabolites. It is concluded that conjugation reaction involving glutathiones and xenobiotic alachlor, a typical phase II reaction, acts as detoxification reaction in the three test plants and this would, in turn, contribute to observed selectivity of alachlor.

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