• 제목/요약/키워드: physiological stress

검색결과 1,369건 처리시간 0.041초

Computational Analysis on Calcium Dynamics of Vascular Endothelial Cell Modulated by Physiological Shear Stress

  • Kang, Hyun-Goo;Lee, Eun-Seok;Shim, Eun-Bo;Chnag, Keun-Shik
    • International Journal of Vascular Biomedical Engineering
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    • 제3권2호
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    • pp.1-9
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    • 2005
  • Flow-induced dilation of blood vessel is the result of a series of bioreaction in vascular endothelial cells(VEC). Shear stress change by blood flow in human artery or vein is sensed by the mechanoreceptor and responsible for such a chain reaction. The inositol(1,4,5)-triphophate($IP_3$) is produced in the first stage to elevate permeability of the intercellular membrane to calcium ions by which the cytosolic calcium concentration is consequently increased. This intracellular calcium transient triggers synthesis of EDRF and prostacyclin. The mathematical model of this VEC calcium dynamics is reproduced from the literature. We then use the Computational Fluid Dynamics(CFD) technique to investigate the blood stream dictating the VEC calcium dynamics. The pulsatile blood flow in a stenosed blood vessel is considered here as a part of study on thrombogenesis. We calculate the pulsating shear stress (thus its temporal change) distributed over the stenosed artery that is implemented to the VEC calcium dynamics model. It has been found that the pulsatile shear stress induces larger intracellular $Ca^{2+}$ transient plus much higher amount of EDRF and prostacyclin release in comparison with the steady shear stress case. It is concluded that pulsatility of the physiological shear stress is important to keep the vasodilation function in the stenosed part of the blood vessel.

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Photosynthetic characteristics and chlorophyll of Vitex rotundifolia in coastal sand dune

  • Byoung-Jun Kim;Sung-Hwan Yim;Young-Seok Sim;Yeon-Sik Choo
    • Journal of Ecology and Environment
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    • 제47권3호
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    • pp.103-116
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    • 2023
  • Background: This study analyzed the physiological adaptations of a woody plant, Vitex rotundifolia, in Goraebul coastal sand dunes from May to September 2022. Environmental factors and physiological of plants growing under field and controlled (pot) conditions were compared. Results: Photosynthesis in plants growing in the coastal sand dunes and pots was the highest in June 2022 and July 2022, respectively. Chlorophyll fluorescence indicated the presence of stress in the coastal sand dune environment. The net photosynthesis rate (PN) and Y(II) were highest in June in the coastal sand dune environment and July in the pot environment. In August and September, Y(NPQ) increased in the plants in the coastal sand dune environment, showing their photoprotective mechanism. Chlorophyll a and b contents in the pot plant leaves were higher than those in the coastal sand dune plant leaves; however, chlorophyll-a/b ratio was higher in the coastal sand dune plant leaves than in the pot plant leaves, suggesting a relatively high photosynthetic efficiency. Carotenoid content in the coastal sand dune plant leaves was higher in August and September 2022 than that in the pot plant leaves. Leaf water and soluble carbohydrate contents of the coastal sand dune plant leaves decreased in September 2022, leading to rapid leaf abscission. Diurnal variations in photosynthesis and chlorophyll fluorescence in both environments showed peak activity at 12:00 hour; however, the coastal sand dune plants had lower growth rates and Y(II) than the pot plants. Plants in the coastal sand dunes had higher leaf water and ion contents, indicating that they adapted to water stress through osmotic adjustments. However, plants growing in the coastal sand dunes exhibited reduced photosynthetic activity and accelerated decline due to seasonal temperature decreases. These findings demonstrate the adaptation mechanisms of V. rotundifolia to water stress, poor soils, and high temperature conditions in coastal sand dunes. Conclusions: The observed variations indicate the responses of the V. rotundifolia to environmental stress, and may reveal its survival strategies and adaptation mechanisms to stress. The results provide insights into the ecophysiological characteristics of V. rotundifolia and a basis for the conservation and restoration of damaged coastal sand dunes.

저수온 스트레스시 자바리, Epinephelus bruneus에서의 vitamin E의 생리적 효과 (Physiological effects of dietary vitamin E on kelp grouper, Epinephelus bruneus under low water temperature stress)

  • 임상구;길현우;이태호;박인석
    • 수산해양교육연구
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    • 제29권3호
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    • pp.636-645
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    • 2017
  • The physiological response of the longtooth grouper, Epinephelus bruneus to low water temperature (LWT) stress while on three different concentrations of dietary vitamin E (1 mg/diet g, 5 mg/diet g, and 10 mg /diet g) were investigated. Plasma cortisol, glucose, and aspartate aminotransferase (AST) levels were higher in the control and sham control compared with the vitamin E supplemented groups. The most addition with vitamin E in the diet (10 mg/diet g) showed the lowest levels of cortisol, glucose, and AST. However, plasma alanine aminotransferase (ALT) showed no significant differences across all experimental conditions (P>0.05). Also the more disposing vitamin E into feeding the better resistance against stress on the LWT was shown through cortisol, glucose, ALT, and AST.

Adaptation of Betula schmidtii Seedling in Coal-mine Field with Different Sewage Sludge Treatment Methods

  • Han, Sim-Hee;Lee, Jae-Cheon;Oh, Chang-Young;Kim, Pan-Gi
    • The Korean Journal of Ecology
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    • 제28권4호
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    • pp.189-192
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    • 2005
  • We tested the field adaptation of Betula schmidtii on the abandoned coal-mine soil with sludge amendment methods for promoting physiological activity of B. schmidtii seedlings under several environmental stress. Sewage sludges were amended to coal-mine soil with B. schmidtii seedlings which grown in the mixture of artificial soil and composted sludge soil before transplanting (before-fertilized treatment, BF) and fertilized with composted sludge after transplanting (after-fertilized treatment, AF). The percent of establishment of seedlings for AF (80.7%) was lower than that for BF (92.7%). Nitrate reductase activity and photosynthetic pigment content were higher in AF than in BF, but malondialdehyde (MDA) content and superoxide dismutase (SOD) activity were lower in AF than in BF These results represent that after-fertilized seedlings increase resistance against physiological stress at field condition using nitrogen source of composted sludge. On the contrary, before-fertilized seedlings were susceptible to environmental stress on abandoned coal-mine soil by exhausting of nitrogen source from composted sludge.

협착된 경동맥 내의 벽전단응력 및 혈관의 탄성적 거동 (Elastic Motion of the Blood Vessel and Wall Shear Stress in Carotid Artery with Stenosis)

  • 김창녕;오택열;최명진;정삼두
    • 한국정밀공학회지
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    • 제22권9호
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    • pp.179-187
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    • 2005
  • The characteristics of blood flow and the interaction between the blood vessel and blood flow play important roles in plaque cap rupture and the growth of atherosclerosis which may lead directly to a heart attack or a stroke. In this study, carotid arteries with different stenoses have been numerically simulated to investigate the wall shear stress(WSS) and the elastic motion of the vessel. Blood flow has been treated as physiological, laminar and incompressible flow. To model the shear thining behavior of the blood, the Carreau-Yasuda model has been employed but the viscoelasticity of blood has not been considered. The results show that the WSS of $severe(75\%)$ stenosis is much higher than those of $25\%\;and\;50\%$ stenosis in the region of stenosis. With the increase in the stenosis thickness, the expansion ratio of the center of the stenosis decreases while the expansion ratio of the upstream region of the stenosis increases.

염분 변화에 따른 무지개송어(Oncorhynchus mykiss)의 삼투조절 유전자 발현변화 (Gene Expression Profiles of Rainbow Trout Oncorhynchus mykiss after Salinity Challenge)

  • 최영광;박흠기;김이경
    • 한국수산과학회지
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    • 제54권5호
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    • pp.676-684
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    • 2021
  • Euryhaline teleost have extraordinary ability to deal with a wide range of salinity changes. To study the seawater adaptability of rainbow trout Oncorhynchus mykiss (body weight 638±54 g, length 38.6±2 cm) to salinity increase fish were transferred from freshwater to 7, 14, 21, 28 and 32 psu and checked for mortality over 5 days. No mortality was observed in 0-32 psu. In fish transferred to 0-32 psu, blood osmolality was maintained within physiological range. The changes of serum enzyme activities (aspartate transaminase, AST and alanine transaminase, ALT) showed no significant level during experimental period. To explore the underlying molecular physiology of gill and kidney responsible for body fluid regulation, we measured mRNA expression of five genes, Na+/K+/2Cl- cotransporter1 (NKCC1), aquaporin3 (AQP3), cystic fibrosis transmembrane conductance regulator (CFTR), glucocorticoid receptor (GR) and growth hormone receptor (GHR) in response to salt stress. Based on our result, rainbow trout could tolerate gradual transfer up to 32 psu for 5 days without mortality under physiological stress. This study suggests to alleviate osmotic stress to fish, a gradually acclimation to increasing salinity is recommended.

Functional Characterization of Mammary Gland of Holstein Cows under Humid Tropical Summer Climates

  • Lu, C.H.;Chang, C.J.;Lee, P.N.;Wu, C.P.;Chen, M.T.;Zhao, X.
    • Asian-Australasian Journal of Animal Sciences
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    • 제16권7호
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    • pp.988-995
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    • 2003
  • Physiological parameters were measured on six primiparous, non-pregnant Holstein cows prior to peak lactation over a 3-month summer season in southwestern Taiwan. The objectives were to characterize heat stress-induced change in functionality of mammary gland under natural climates of tropical summer and to establish physiological indices applicable to this environment in referring to this change. Environmental and physiological readings, milk and blood samples were taken at 15:00 h biweekly for totally five time points during the study. Climate readings showed that the afternoon humidex value reached the highest (53.5) around mid summer. Rectal temperature of cows taken simultaneously varied between $38.26^{\circ}C$ and $40.02^{\circ}C$ in parallel to humidex. Milk production declined drastically from 29.2 to 22.2 kg/d the first month entering summer but leveled up at end of the summer season suggesting effects exerted by heat stress rather than stages of lactation. Lactose content decreased linearly (p<0.05) with times in summer, from 4.69 to 4.38%. On the other hand, activity of N-acetylglucosaminidase (NAGase) in milk increased linearly to over two folds (p<0.05) during the same intervals. Elevations of fractional constituent of BSA in whey protein and serum cortisol level were also noticed in the course. Measurement of arteriovenous concentration (A-V) difference across the mammary gland demonstrated net uptake of glucose and net release of urea throughout the study period. The amount of urea released from mammary gland increased (p<0.05) progressively from 1.54 to 7.76 mg/dl during summer. It is concluded that gradual regression of mammary gland occurred along the humid tropical summer season. This regression is likely initiated through elevation of body temperature, which is irreversible above certain point. The increased release of urea from mammary gland during heat stress suggests its potential role as an early indicator of suboptimal mammary function.