• Journal of East-West Nursing Research
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• v.7 no.1
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• pp.18-31
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• 2002

This study, as a basic research to manage a Chinese Medicine Health Promotion Center by way of showing an example, is a before and after experiment research for simple group to verify a difference with cholesterol, health status and perception of health in order to confirm a effectiveness of diet and regimen according to the 4th status of physical constitution. Research object was chosen of 42 persons who operate a physical constitutional dietary regimen among them after selecting professors and clinical nurses (55 persons) majoring in the science of nursing who participated in Chinese Medicine-oriented Nurse Training Course from Aug. of 2001 to Feb. of 2002 all over the country. Diagnostic tools for physical constitution was used of the questionary that is currently consisted of physical constitution grouping test in Eastern & Western Diagnose Center of K Medical Center, and rating of health status was used of the tool that standardized CMI(Cornell Medical Index) to be available for Korean, and perception measurement for health status was used of a visual analogue scale for the health status that each one perceive personally, and physiological status was measured of cholesterol in blood. Analysis for the collected data was carried out by percentage, $X^2$ test, paired t-test according to research object by using SPSS, and the results of this study are as follows. 1) There was no difference with cholesterol before or after the experiment for objects. As a result of estimation about difference with health status by areas before or after the experiment, there are more improved result in eyes, ears, digestive organs, bones and sinews organs, frequency of a disorder, habit, adaptation status, angry, healthy status than before the experiment. As a whole, after the experiment the health was more improved than before the experiment. As the result to inspect a difference of health perception between before and after experiment, after the experiment the health perception level was improved than before, however there was no meaningful differences. 2) As the result to inspect a difference of cholesterol between before and after experiment according to object's physical constitution, in the case of So-yang-in(a person with the minimum male: according to the male and female principles(the sun and the moon)) among the 4th status of physical constitution there was only meaningful difference statistically, however, after the experiment their cholesterol's value was increased. As the result to inspect the difference of health status between before and after the experiment according to physical constitution, all of Ta-um-in(a person with maximum the female), So-yang-in(with the minimum male), So-um-in(with the minimum female) had a meaningful difference before and after the experiment, which means that in all case by physical constitutional groups, after the experiment their health status was more improved than before the experiment. As the result to inspect a difference of health perception between before and after according to physical constitutions, in the case of Tae-um-in and So-um-in, average score after the experiment was risen than before the experiment so that it means that the level of health perception was improved, however, there was no meaning statistically. According to the above results, if continuous diet and regimen by each physical constitutions could be implemented, it is certain that the health could be maintained and promoted. And, what we are healthy is for oneself to feel it subjectively. However, I think that cholesterol score in blood that we can view objectively could be changed distinctly if we can implement a strict diet and regimen. Accordingly, it is necessary for a method and period of experiment to be more strict and longer. According to the above results, I would like to suggest as follows. 1) In order to understand health status by Korean's physical constitutions and to generalize it, these research will be repeated against much more objects that could be selected by proper grouping method to consider a representative. 2) It is necessary for a research to inspect health status by physical constitution by developing a health status measurement tool that has higher confidence and propriety based on physical constitutional theory.

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1579-1584
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• 2008

Anemia are often detected among many patients who are treated in oriental traditional medicine hospitals in Korea through blood tests which are taken to diagnose the patients' general state, and most of them are diagnosed to have iron deficiency anemia. This study amis the effect of herbal medicine on the RBC count, hemoglobin, hematocrits and indices of red blood cells such as mean corpuscular volume(MCV), mean corpuscular hemoglobin(MCH), mean corpuscular hemoglobin concentration(MCHC), serum iron, and total iron binding capacity(TIBC) among patients who were treated in oriental medical hospital and diagnosed to have anemia through blood tests other than main symptoms. The subjects of this study were 29 patients(male 7, female 22, mean age: 56.24${\pm}$19.11years) who were chosen ones, they has below hemoglobin 12 gm/dL, serum iron 70 IU/L, MCV 80 fL on laboratory screening test when they first visits our hospital. As a result, the volume of red blood cell(RBC), hematocrit(HCT), and hemoglobin(Hb) in peripheral blood increased from $348.27{\pm}8.95{\times}10^5$ /UL, 28.82${\pm}$0.56%, 9.24${\pm}$0.22 g/dL to $375.62{\pm}9.11{\times}10^5$ /UL, 31.36${\pm}$0.68%, 10.31${\pm}$0.25 g/dL comparing before and after oriental medicine treatment. After the treatment, MCH, MCHC, and MCV also increased from 26.98${\pm}$0.84 Pg, 32.62${\pm}$0.45 g/dl, 79.26${\pm}$0.17 fL to 27.74${\pm}$0.74 Pg, 32.84${\pm}$0.33 g/dl, 84.15${\pm}$1.67 fL. There was statistically significant increase in MCH, MCV(P<0.05); however, there was no significant change in MCHC(P>0.05). The amount of iron in blood increased from 32.96${\pm}$4.20 ug/dL to 67.22${\pm}$7.18 ug/dL, which showed a statistically significant increase(P<0.05). On the other hand, TIBC decreased from 325.08${\pm}$13.97 ug/dL to 315.61${\pm}$18.79 ug/dL, which was not a statistically significant change(P>0.05). Like this, all the patients showed favorable results in anemia based on the measurements of indices of red blood cells except MCHC. According to the classification of herbal medicines, in strengthening energy group(補氣), replenishing blood group(補血), and replenishing both energy and blood group(補氣血), the volume of RBC, HCT, and hemoglobin showed significant increases. Furthermore, in herbal medicines strengthening blood(補血) and replenishing both energy and blood group(補氣血), serum iron also relatively increased compare to strengthening energy group(補氣).

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1359-1367
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• 2008

DSOM(Diagnosis System of Oriental Medicine) was made as a computerized assistant program for oriental medicine doctors to be able to diagnose with statistical basis. Then DSOM uses questionnaires filled out by subjects without enough explanatory guide. If the subject misunderstand the meaning of the passages, we might not rely on that result. So I designed this study to investigate the diagnostic correspondent rates between DSOM and practitioners. First, let the respondents answer to DSOM(DSOM-Ⅰ for the rest). After that, three doctors diagnosed the respondents and marked how much they had symptoms about 16 pathogenic factors in the score range 0${\sim}$5('0' means they didn't have that symptom, '1' means they had that symptom but mild, '3' means they had that symptom moderately, '5' means they had that symptom severely. And let the respondents answer to DSOM(DSOM-Ⅱ for the rest) again. Finally, we investigated the correspondent rates of diagnosis between DSOM-Ⅰ,Ⅱ and doctors'. We obtained conclusions as following. In the comparison of output frequency rate of the pathogenic factors, the difference between DSOM-Ⅰ and Ⅱ was 1%. In the correspondent rates of diagnosis between DSOM-Ⅰ,Ⅱ and doctors', In DSOM-Ⅰ and Ⅱ answered by subjects two times respectively, the correspondent rate was highest in insufficiency of Yang(陽虛) and liver(肝) as 93.2%, lowest in damp(濕) as 69.5% and showed 81.9% in all 16 pathogenic factors mean. In DSOM-Ⅰ and Ⅱ, and Doctors' diagnose, they showed the complete correspondent rates of 15.3${\sim}$61.0%, 15.3${\sim}$59.3% in individual pathogenic factor, 36.5%, 37.3% in all 16 pathogenic factors mean each, and within ${\pm}$1 errorrange, they showed the correspondent rates of 32.2${\sim}$93.2%, 35.6${\sim}$89.8% in individual pathogenic factor, 67.6%, 67.3% in all 16 pathogenic factors mean each, and within ${\pm}$2 error range, they showed the correspondent rates of 62.7${\sim}$98.3%, 71.2${\sim}$100% in individual pathogenic factor, 85.1 87.6%% in all 16 pathogenic factors mean each. In the correspondent rates of the severe case, In the cases that the Doctors' diagnostic score mean was over 3(the severity of disease is middle), there were deficiency of qi(氣虛), stagnation of qi(氣滯), blood stasis(血瘀), damp(濕), liver(肝), heart(心), spleen(脾) and they all showed the correspondent rates of over 60 except blood stasis(血瘀). In the cases that the weighed pathogenic factor was above 9, the correspondent rates were 50${\sim}$100%. deficiency of qi(氣虛), blood-deficiency(血虛), stagnation of qi(氣滯), blood stasis(血瘀), insufficiency of Yin(陽虛), insufficiency of Yang(陽虛), coldness(寒), heat (熱), damp(濕), dryness(燥), liver(肝), heart(心), spleen(脾), kidney(腎), phlegm(痰).

• Journal of Physiology & Pathology in Korean Medicine
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• v.22 no.6
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• pp.1626-1632
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• 2008

Among patients who are receiving treatments at an oriental medical hospital for various symptoms and diseases, patients whose main disease is accompanied by metabolic syndrome with abnormal liver function. This research was performed in order to observe the progression of changes in the liver function and serum lipid profile after the oriental medical treatments to patients who have been receiving oriental medical treatment for various other diseases and have been diagnosed as having metabolic syndrome at their first visit to the hospital based on NCEP ATP III diagnosis criteria and WHO Asia Pacific region criteria. Total number of subject patients were 39cases(mean age:55.58${\pm}$2.09 years) which had 20 male and 19 female. For the references for hepatic enzyme levels and blood lipid profile were measured in before treatment and four times after treatments(every 2.31${\pm}$06.17 weeks). Serum AST was 48.86${\pm}$7.46 IU/L before oriental medical treatment. After the treatment, 40.63${\pm}$4.69, 43.12${\pm}$5.46, 37.82${\pm}$4.52 IU/L were measured where although the level decreased to the normal level compared to pre-treatment, the value was not significant statistically(P>0.05). ALT was 66.26${\pm}$11.01 IU/L before oriental medical treatment. After the treatment 62.10${\pm}$8.20, 61.10${\pm}$8.76, 43.79${\pm}$5.68 were measured where although the level decreased, abnormally high level was maintained. The last result was significant statistically(P<0.05) compared to pre-treatment. ALP was 193.06${\pm}$14.20 IU/L before oriental medical treatment. After the treatment, 176.80${\pm}$6.48, 177.46${\pm}$11.81, 162.41${\pm}$9.06 where although compared to pre-treatment the last result was significant statistically(P<0.05), the change was within the normal range. ${\gamma}$-GGT was 87.83${\pm}$12.59 IU/L before oriental medical treatment. After the treatment, progressively near normal level was achieved with 118.73${\pm}$46.45, 85.03${\pm}$17.12, 70.64${\pm}$10.93 and the last result was statistically significant compared to pre-treatment (P<0.05). Blood triglyceride was 217.63${\pm}$32.18 mg/dL before oriental medical treatment. After treatment 215.09${\pm}$22.18, 189.93${\pm}$22.44, 191.22${\pm}$18.51 where abnormal values continued even after treatment although results was not statistically significant compared to pre-treatment(P>0.05). Total-cholesterol was 197.28${\pm}$9.24 mg/dL before oriental medical treatment, after treatment 201.55${\pm}$11.13, 186.87${\pm}$8.77 and 186.68${\pm}$7.61 were measured that results were not statistically significant compared to pre-treatment(P>0.05). HDL-cholesterol was 41.88${\pm}$2.38 mg/dL before oriental medical treatment, after treatment 48.75${\pm}$4.22, 44.10${\pm}$1.91, 48.00${\pm}$2.06 the results were not statistically significant compared to pre-treatment(P>0.05). LDL-cholesterol was 111.66${\pm}$13.08 mg/dL before oriental medical treatment, after treatment 109.94${\pm}$10.18, 101.79${\pm}$8.63, 104.00${\pm}$6.98 the results were not statistically significant compared to pre-treatment(P>0.05). With such results, even if common oriental medical treatments were given to metabolic syndrome patients with abnormal liver function, the liver function was confirmed not to be aggravated, and the concentration of lipids in the blood was confirmed not to be affected in most patients.

• Applied Microscopy
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• v.25 no.2
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• pp.53-64
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• 1995

This study was designed to investigate the morphological alterations of zonula occludens, macula adherences and gap junctions between the hepatocytes in the fasting conditions. Animals (Sprague Dawley, $250{\sim}280g$) were divided into two groups: normal and fasting. The latter were fasted for eight days prior to sampling. Liver tissues were sectioned and replicated after freeze fracturing for the transmission electron microscopy. In the normal rat liver, the interhepatocellular space at the area of some zonula occludens appeared to be widened in thin sections. On the freeze fracture replicas., the zonula occludens appeared as an anastomosing network of $2{\sim}4$ strands or grooves on P or E faces. Free ends and fragments of the strands were observed. In the rat fasted for eight days, the hepatocytes were diminished in size and the organelles were decreased in number and size. The intercellular space was wide at many areas of zonula occludens in thin section. On the freeze fracture replicas, the zonula occludens showed diminution or disappearence of anastomosing network of strands or grooves. Free ends and small fragments of the strands or grooves were frequently encountered. The macula adherens was markedly increased in number in thin sections, although they could not be found on the freeze fracture replicas. The gap junctions were increased in number in thin sections. Small aggregations of the intramembranous particles appeared with larger ones on the freeze fracture replica. The evidences may suggest the followings: (1) The disassembly of zonula occludens in the fasting states is led from the diminished mechanical stress on the luminal surface of bile canaliculus with the impaired secretion of bile components from the hepatocytes. (2) The increase of macula adherens is necessary to maintain the liver parenchyma integrity in the fasting state which leads the hepatocyte to be diminished and finally the intercellular space to be separated. (3) The rise in both number and size of gap junctions is owing to the need of increasing intercellular communication between the hepatocytes during the fasting. (4) The alteration of zonula occludens is easily led by the physiological condition of hepatocytes even in the normal ones.

• Journal of Life Science
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• v.18 no.7
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• pp.963-967
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• 2008

The biological activities of water extracts from different fruit parts, such as peel, flesh, and placenta, of oriental melon were investigated. The concentrations of total phenolic in fruit extracts were $816.37\;{\mu}g/ml$, $385.23\;{\mu}g/ml$, and $925.56\;{\mu}g/ml$, respectively. Whereas the total flavonoid content in the peel extracts was $231.21\;{\mu}g/ml$, those in the extract of flesh and placenta were $8.16\;{\mu}g/ml$ and $36.07\;{\mu}g/ml$, respectively. The DPPH free radical scavenging activity of each fruit extract at 10,000 ppm was 34.84% for peel, 10.70% for placenta and 9.26% flesh. The ABTS radical cation decolorizing activity of each fruit extract at 10,000 ppm was in fruit extracts were 72.92% for peel, 48.0% for flesh and 74.31% for placenta. In addition, xanthine oxidase inhibitory activity, ${\alpha}-Glucosidase$ inhibition activity, and tyrosinase inhibition activity of the peel extracts appeared to be higher than those of placenta and flesh. Taken together, these results indicated that the peel part of oriental melon contained higher level of total flavonoid content, and several physiological activities including antioxidation, ${\alpha}-Glucosidase$ inhibition activity, and tyrosinase inhibition activity than did the flesh and placenta parts, and suggested that the peel might have a potential to be applicable as a source for functional foods.

• Korean Journal of Agricultural and Forest Meteorology
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• v.11 no.1
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• pp.13-18
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• 2009

Accurate solar radiation data are critical to evaluate major physiological responses of plants. For most upland crops and orchard plants growing in complex terrain, however, it is not easy for farmers or agronomists to access solar irradiance data. Here we suggest a simple method using a sun-slope geometry based topographical coefficient to estimate daily solar irradiance on any sloping surfaces from global solar radiation measured at a nearby weather station. An hourly solar irradiance ratio ($W_i$) between sloping and horizontal surface is defined as multiplication of the relative solar intensity($k_i$) and the slope irradiance ratio($r_i$) at an hourly interval. The $k_i$ is the ratio of hourly solar radiation to the 24 hour cumulative radiation on a horizontal surface under clear sky conditions. The $r_i$ is the ratio of clear sky radiation on a given slope to that on a horizontal reference. Daily coefficient for slope correction is simply the sum of $W_i$ on each date. We calculated daily solar irradiance at 8 side slope locations circumventing a cone-shaped parasitic volcano(c.a., 570m diameter for the bottom circle and 90m bottom-to-top height) by multiplying these coefficients to the global solar radiation measured horizontally. Comparison with the measured slope irradiance from April 2007 to March 2008 resulted in the root mean square error(RMSE) of $1.61MJ\;m^{-2}$ for the whole period but the RMSE for April to October(i.e., major cropping season in Korea) was much lower and satisfied the 5% error tolerance for radiation measurement. The RMSE was smallest in October regardless of slope aspect, and the aspect dependent variation of RMSE was greatest in November. Annual variation in RMSE was greatest on north and south facing slopes, followed by southwest, southeast, and northwest slopes in decreasing order. Once the coefficients are prepared, global solar radiation data from nearby stations can be easily converted to the solar irradiance map at landscape scales with the operational reliability in cropping season.

• Korean Journal of Animal Reproduction
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• v.23 no.2
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• pp.175-180
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• 1999

Blood chemical values and sex steroid hormone concentration of repeat breeder and reproductive disorder in Hanwoo, which were bred in Chunchon areas, were examine to establish the basic physiological maker. The concentrations of albumin, blood urea nitrogen(BUN), calcium(Ca), cholesterol, creatinine, glucose, phosphorous, total protein and triglycerides in repeat breeder and reproductive disorder were 3.28$\pm$0.05 and 3.27$\pm$0.04 g/㎗, 11.07$\pm$0.61 and 12.69$\pm$0.88 mg/㎗, 9.98$\pm$0.10 and 9.56$\pm$0.11mg/㎗, 105.75$\pm$3.57 and 126.78$\pm$5.66mg/㎗, 1.62$\pm$0.05 and 1.65$\pm$0.09 mg/㎗, 67.46$\pm$9.07 and 76.97$\pm$3.76mg/㎗, 6.13$\pm$0.36 and 6.11$\pm$0.26mg/㎗, 5.82$\pm$0.08 and 6.18$\pm$0.08 g/㎗ , 12.82$\pm$1.46 and 15.19$\pm$1.61mg/㎗, respectively. Among the blood chemical values, Ca, cholesterol and inorganic phosphorous levels in repeat breeder and reproductive disorder were slightly higher than those in normal cows. The progesterone levels of pregnancy(0.18~6.56 ng/$m\ell$) and non-pregnancy(0.15~5.46ng/$m\ell$) cows at estrus cycle were showed similar trends from 0 day to 18 days. Those of pregnancy cows were increased gradually from 18 days to 180 days(5.90~7.51 ng/$m\ell$), while those of non-pregnancy cows were dramatically drop at 21 days (0.18$\pm$0.03mg/㎗). The concentrations of estrogen and progesterone in repeat breeder and reproductive disorder were 57.42$\pm$5.03, 56.76$\pm$8.73 pg/$m\ell$ and 6.26$\pm$0.83, 9.41$\pm$1.85 ng/$m\ell$ . respectively. These results indicate that blood chemical values in repeat breeder and reproductive disorder were not greatly difference in normal cows except for Ca, cholesterol, BUN and inorganic phosphorous. Since the progesterone levels in repeat breeder and reproductive disorder was maintained to the luteal phase levels of normal cows, it may cause of failing of conception and next estrus cycle.

• Food Science and Preservation
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• v.12 no.3
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• pp.282-286
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• 2005

This study was to investigate the content of total phenolic compounds, electron donating ability, nitrate-scavenging effect, and angiotensin converting enzyme(ACE) inhibition effect of medicinal plant liquors prepared from Cnidium officinale, Angelica gigas, Pueraria thunbergiana(root), Pueraria thunbergiana (flower), and Glycyrrhiza uralensis. Physicochemical characteristics of the medicinal plant liquors are as follows: pH $5.65{\sim}6.36$; reducing sugar, $0.13{\sim}0.45\%$. The highest value of total phenolic compounds was found in liquor prepared with Pueraria thunbergiana(root) as $23.9{\sim}54.3\;mg\%$. The electron donating ability of liquor prepared with Pueraria thunbergiana showed the highest value($67.4{\sim}85.3\%$) among the liquors, and its nitrite scavenging ability($24.56{\sim}78.3\%$) showed the highest value than those of other medicianal plant liquors. ACE inhibitory activity showed the highest value in liquor prepared with Cnidium officinale.

• Journal of The Korean Society of Grassland and Forage Science
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• v.5 no.1
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• pp.45-52
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• 1985

Phytotron and field experiments were conducted to determine the influence of morphological development and environmental temperature on synthesis, translocation and accumulation behaviour of Fructosan, Monoand Disaccharose in corn cv. Blizzard and fodder sorghum cv. Sioux and Pioneer 931 at Munich technical university. Sorghum and maize plants were grown for 42 days at 4 temperature regimes (30/25, 25/20, 28/18 and 28/8 dog C) and mid-summer sunlight over 13-h days. The obtained results are summarized as follows: 1 Non-structural carbohydrates in maize and sorghum were accumulated mainly as Mono- and Disaccharose. The concentrations of Mono- and Disaccharose were increased markedly after differentiation of growing points and shown at early milk stage the highest contents with 27.8-29.1% and 16.8-20.4% for maizeand sorghums respectively. 2. Non-structural carbohydrates were accumulated mainly in stalk. However, during the late maturity the most of Mono- and Disaccharose were translocated into grain and reserved as starch. The increase of starch was associated with decrease of total non-structural carbohydrates. 3. Fructosan synthesis was not affected by morphological changes and environmental factors, which shows a value of 1.5-2.5% in whole stage of maize and sorghum. 4. Sorghum and maize plants were shown to have a great photosynthetic rates to high temperature. Reserved Mono- and Diaaccharose were, however, declined when temperature exceeded 30 dog C. Under cold stress at l8/8 deg C non-structural carbohydrates were not translocated and also were accumulated in leaves too much that cause to restrict of photosynthesis. 5. Net Energy Lactation (NEL) of sorghum and maize were directly associated with synthetic rates of non-structural carbohydrates, especially Mono- and Disaccharose. The highest values of NEL were found at physiological maturity stage with 6.6- 6.9 MJ and 5.7-6.0 MJ-NEL/kg for maize and sorghum respectively.