• Journal of the Korean Society of Food Science and Nutrition
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• v.33 no.6
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• pp.1068-1073
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• 2004

Response surface methodology (RSM) was used to monitor characteristics of hot water extracts from Hericium erinaceus. A central composite design was applied to investigate the effects of independent variables, extraction temperature (X$_1$), sample ratio (X$_2$) and extraction time (X$_3$) on dependent variables such as soluble solid (Y$_1$), total phenols (Y$_2$), crude protein (Y$_3$) and electron donating ability (Y$_4$) of the extracts. As the sample ratio increased, the soluble solid content increased, while extraction temperature played a minor role. As a whole total phenols and crude protein contents increased with increasing the sample ratio. Electron donating ability increased in proportion to extraction temperature and sample ratio, which didn't increase at certain period. Then ranges of optimum extraction conditions for maximized physicochemical properties were 91.5∼96.5$^{\circ}C$ in extraction temperature and 3.5∼4.2 g/100 mL in sample ratio. Predicted values at the optimized conditions were coincident with experimental values.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.53 no.6
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• pp.834-841
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• 2020

Laver Pyropia sp. is the most exported seafood in Korea and is one of the most consumed edible seaweeds. The export of laver has been centered on seasoned laver products; of note, laver snack products coupled with rice papers or glutinous rice paste are becoming more popular in Western countries. These laver snacks are manufactured using a deep frying process. Therefore, the frying conditions affect the physicochemical and sensory characteristics of fried laver snacks. In this study, we optimized the deep-frying conditions for laver snacks with gelatinized rice dough using a response surface methodology. The frying temperature (X1, 190-220℃) and frying time (X2, 10-30 sec) were selected as independent variables; the dependent variables were the overall acceptance (Y1, points), hardness (Y2, N), and oil content (Y3, %). The optimal values of the frying temperature (X1) and time (X2) as per the overall acceptance (Y1) were 208℃ and 23.1 sec, respectively. The predicted overall acceptance (Y1=8.0 points), hardness (Y2=98.74N), and oil content (Y3=27.69%), were similar to the experimental values under the optimal conditions. Overall, the acceptance was inversely proportional to hardness; the decrease in hardness caused an increase in the crispiness of the laver snacks.

• Journal of Pharmaceutical Investigation
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• v.34 no.1
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• pp.1-14
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• 2004

A promising approach to the development of a new single-step vaccine, which would eliminate the requirement for multiple injections, involves the encapsulation of antigens into microspheres. Biodegradable poly(lactide-co-glycolide) (PLGA) microspheres gave us a bright insight for controling antigen release in a pulsatile fashion, thereby mimicking two or tree boosting injections. However, in spite of the above merits, the level of immunization induced by a single-shot vaccination is often lower tan two doses of alum-adsorbed antigen. Therefore, optima modification of the microsphere is essential for the development of single-step vaccines. In the review, we discuss the stability of antigen in microsphere, safety and non-toxic in human and encapsulation technology. Also, we attempted to outline relevant physicochemical properties on the immunogenicity of microsphere vaccine and attainment of pulsatile release pater by combination of different microsphere, as well as to analyze immunological data associated with antigen delivery by microsphere. Although a lot of variables are related to the optimized microsphere formulation, we could conclude that judicious choice of proper polymer type, adjustment of particles size, and appropriate immunization protocol along with a suitable adjuvant might be a crucial factor for the generation of long-lasting immune response from a single-step vaccine formulation employing PLGA microsphere.

• Advances in environmental research
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• v.10 no.1
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• pp.43-57
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• 2021

Evaluation of groundwater quality is vital due to its diverse use for several purposes. In the present study, groundwater quality and suitability from the Peshawar basin, Pakistan, were evaluated for drinking and irrigation purposes. The water samples were analysed for major cations (Ca, Mg, Na and K) and anions (chloride, bicarbonate and sulphate) along with other physicochemical parameters (pH, electrical conductivity, total dissolved solids, and total hardness). About 95% of the water samples were found to be within the WHO, US-EPA and Pak-EPA permissible levels for drinking purposes. Seventy percent (70%) of the water samples belonged to the hard water category. Irrigation water quality parameters, such as, chloride, residual sodium bicarbonate, sodium adsorption ratio, percent sodium, magnesium adsorption ratio, Kelly's ration and permeability index were evaluated which demonstrated that the groundwater was highly to moderately suitable for irrigation. A correlation study was conducted to find out the mutual associations among the variables. Piper diagram indicated the overall chemical nature of the study area was calcium-magnesium bicarbonate type. Cluster analysis revealed mutual apportionment of various parameters in the groundwater of the Peshawar basin, Pakistan.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.41 no.6
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• pp.427-434
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• 2008

Processing of collagen from yellowfin tuna (Thunnus albacares) abdominal skins was optimized by response surface methodology and central composite design. The values of independent variables at optimal conditions were NaOH concentration: 0.5 N, NaOH treatment time: 36.2 hr, pepsin concentration: 1:4.9 ratio (0.245%, w/v), and digestion time: 48.1 hr, respectively. The collagen content estimated under optimal conditions was 33.1%, and the actual experimental collagen content was 32.3%. Physicochemical properties of collagen from yellowfin tuna abdominal skin were investigated by amino acids analysis, SDS-PAGE, FT-IR, viscosity and denaturation temperature. Amino acids content of the collagen was 21.0%. SDS-PAGE pattern of the collagen showed two different $\alpha$-chain (${\alpha}_1$- and ${\alpha}_2$- chain), $\beta$-component and $\gamma$-component. The spectrum of FT-IR of the collagen showed wavenumber at 3,434, 1,650, 1,542 and $1,235\;cm^{-1}$ representing the regions of amide A, I, II and III, respectively. Relative viscosity of the collagen decreased continuously on heating up to $32^{\circ}C$, and the rate of decrease was retarded in the temperature range of $35-50^{\circ}C$. Denaturation temperature (Td) of the collagen solution (0.06%, w/v) was $31^{\circ}C$ and was lower than calf skin collagen ($35^{\circ}C$).

• Journal of the Korean Society of Food Science and Nutrition
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• v.37 no.8
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• pp.1018-1024
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• 2008

The objective of this study is to compare the physicochemical properties and release characteristics of red ginseng (A) and tissue cultured mountain ginseng (B) extruded tissue cultured mountain ginsengs at barrel temperatures 110 (C) and $120^{\circ}C$ (D) to produce tissue cultured mountain ginseng-like comercial red ginseng by extrusion process. Extrusion process variables, water content and screw speed were fixed at 25% and 200 rpm, respectively. In the results, reducing and total sugar content were found to be relatively higher in A. The acidic polysaccharides content of B was the lowest among the ginseng samples. Acidic polysaccharide was increased 3 times by extrusion process. A and B were three times higher at maximum than C and D in polyphenolic compound. Polyphenolic compound content was relatively low by extrusion of ginsengs. Amino acid contents of B, C and D were $35{\sim}42\;{\mu}g/mL$; in contrast, A contained $25\;{\mu}g/mL$. The crude saponin content of C and D were higher than A and B.

• Journal of the Korean Society of Food Science and Nutrition
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• v.35 no.4
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• pp.499-506
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• 2006

Response surface methodology was used to investigate the cookie manufacturing process with black rice flour. A three- variable, three-level central composite design was employed where the independent variables were the amount of black rice flour ($0{\sim}20%$), baking time ($10{\sim}14 min$), and sugar type (sucrose, aspartame and oligosaccarides). pHs of dough and cookie tended to increase with the addition of black rice flour. Moisture content of dough slightly increased with tile addition of black rice flour but nearly affected by baking time. Spread factor increased with the addition of black rice flour and it was more evident in the samples prepared with sucrose. L*-value decreased but a*-value increased significantly with the addition of black rice flour. Generally the amount of black rice flour in the sample did not affect the textural characteristics of cookie. As the amount of black rice and baking time increased, sensory flavor became stronger. Sensory sweetness as well as hardness increased but sensory color became darker with the addition of black rice flour. In addition, the response surface models developed in this study for most of physicochemical and sensory characteristics of black rice cookie were adequate.

• Journal of the Korean Society of Food Science and Nutrition
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• v.36 no.5
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• pp.629-635
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• 2007

Response surface methodology (RSM) was used for the analysis and optimization of the production process of Kochujang added with Maesil extract. The process variables were the amount of Maesil extract ($0{\sim}8%$) and red pepper powder ($6{\sim}10%$). pH and all color characteristics (L*-, a*-, and b*-value) decreased but titratable acidity and water activity increased with the addition of Maesil extract. Amino nitrogen content appeared to decrease with the addition of red pepper powder. The highest sensory flavor score was obtained when 2.20% Maesil extract and 8.62% red pepper powder were blended, the highest sensory taste score with 6.63% Maesil extract and 9.50% red pepper powder, and the highest sensory color score with 7.80% Maesil extract and 8.62% red pepper powder respectively. The point chosen as representative of the optimal area corresponds to $X_1=3.60%,\;X_2=10%\;and\;X_1=4.08%,\;X_2=9.96%$ for physicochemical and sensory quality, respectively.

• Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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• v.14 no.4
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• pp.331-341
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• 2016

To evaluate the physicochemical and radiological properties of raw materials and by-products in domestic distribution, about 220 samples with 16 species were prepared. We measured the energy spectrum and the chemical content, such as U, Th, and K, using a $LaBr_3$ scintillation detector and ED-XRF. In addition, HPGe detector was used to analyze the radioac-tivity of $^{234}Th$, $^{234}mPa$, and $^{214}Bi$ in uranium decay series and $^{228}Ac$, $^{212}Pb$, and $^{208}Tl$ in thorium decay series, and $^{40}K$. The correlation between characteristic variables, such as the count rate in several ROIs, chemical content, and radioactivity, was assessed to infer the radioactivity of natural radionuclides through a rapid screening method. Based on the results, a characteristic database for raw material and by-product in domestic distribution was established and it will provide useful information in the analysis procedure and improve the accuracy and reproducibility in the analysis of natural radionuclides.

• Korean Journal of Food Science and Technology
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• v.49 no.4
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• pp.444-452
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• 2017

This study aims to investigate the physicochemical properties of the extruded rice snack with added the containing mealworm. Adding the mealworm addition enhanced protein and unsaturated fatty acid contents, which are insufficient in rice. As the extrusion process variables, the death temperatures and moisture content were set to at respective 130 and $140^{\circ}C$, and 20 and 25%, respectively. The expansion ratio increased as with death temperature, whereas the increased moisture content decreased. The nitrogen solubility index and protein digestibility index increased with the added mealworm content of mealworm increased. As mealworm content and death temperature, and moisture content increased, DPPH radical scavenging activity significantly increased but the rancidity decreased. As the result of this study, confirmed that the addition of mealworm to the extruded rice snack was the confirmed as the effective process to improve nutrition, and antioxidation. Also, death temperature and moisture content have an effect on softened the texture of extruded rice snack.