• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.8
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• pp.1193-1199
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• 2003

As a result of resistant starch yield depending on heating temperature, moisture content, storage temperature and heating-cooling cycle with RSM (response surface methodology), high amylose corn starch (46%) was appeared higher than normal corn starch in the yield (22%). At the high amylose corn starch, optimum conditions for resistant starch formation were 6 times of heating-cooling cycle, 108$^{\circ}C$ heating temperature and 67% moisture content at the 2$0^{\circ}C$ storage temperature, which resulted in 25% yield with these experiment conditions. Affecting factor for the resistant starch formation was arranged according to heating -cooling cycle, moisture content, heating temperature and storage temperature. Raw corn starch granule was destructive and appeared a porous reticular structure by the resistant starch formation. Color became dark and increased yellowness by caramelization during heating processing. Heating-cooling processing was the result of decreased hardness, cohesiveness, springiness and gumminess.

• Food Science of Animal Resources
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• v.18 no.2
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• pp.115-124
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• 1998

This study was carried out investigate hygienic conditions of domestic packing plant for export and the shelf-life by Japanese standards. vacuum packaged chilled port loins provided by five packers(A, B, C, D and E) were transported to Japan in the same condition, Four packers(A, B, C adn d) produced the samples as hygienic as possible and one packer produced loins in the traditional hygienic condition, In TTC(2,3,5-triphenyl tetrazolium chloride) test all samples showed the negative reaction from the date of the quarantine(14 days after slaughtering) to 50 days The numbers of total plate count(TPC) of A, B, C and D packers that were below 108CFU/g until 50 days were accepted by Japanese Standards but TPC of E packer was over 107CFU/g at 30 days. VBN values increased sig-nificantly(P<0.01) in samples of A,B and D packers in the samples of C packer and E packer at 5% and 0.1% level respectively. The pH values of the vacuum packaged chilled pork for export at 14 days after slaughtering were 5.43∼6.00 In sensory evaluation, A, B, C and D packers which improved hygi-enic condition produced the products with good color and appearance. low drip until 40 days storage at $0^{\circ}C$ But as the storage time increase off-flavor occured at 40days in A and D packer and at 50days in B and C packer. In conclusion according to the above results of microbiological physicochemical and sensory evalution the edible periods of Korean vacuum package chilled porks may be estimated to 40∼50 days for the packers who improved hygienic condition and the shelf lives were calculated as 32∼40 days from edible period by a factor of 0.8(safety coefficient) while that of the pork from E packer which was produced in the traditional hygienic condition was estimated to 30 days after slaughtering at $0^{\circ}C$ Therefore the shelf-life of the pork of E packer would be only 24 days.

• International Journal of Oral Biology
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• v.42 no.2
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• pp.63-70
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• 2017

Selecting an appropriate antigen with optimal immunogenicity and physicochemical properties is a pivotal factor to develop a protein based subunit vaccine. Despite rapid progress in modern molecular cloning and recombinant protein technology, there remains a huge challenge for purifying and using protein antigens rich in hydrophobic domains, such as membrane associated proteins. To overcome current limitations using hydrophobic proteins as vaccine antigens, we adopted in silico analyses which included bioinformatic prediction and sequence-based protein 3D structure modeling, to develop a novel periodontitis subunit vaccine against the outer membrane protein FomA of Fusobacterium nucleatum. To generate an optimal antigen candidate, we predicted hydrophilicity and B cell epitope parameter by querying to web-based databases, and designed a truncated FomA (tFomA) candidate with better solubility and preserved B cell epitopes. The truncated recombinant protein was engineered to expose epitopes on the surface through simulating amino acid sequence-based 3D folding in aqueous environment. The recombinant tFomA was further expressed and purified, and its immunological properties were evaluated. In the mice intranasal vaccination study, tFomA significantly induced antigen-specific IgG and sIgA responses in both systemic and oral-mucosal compartments, respectively. Our results testify that intelligent in silico designing of antigens provide amenable vaccine epitopes from hard-to-manufacture hydrophobic domain rich microbial antigens.

• Bulletin of the Korean Chemical Society
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• v.28 no.11
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• pp.1951-1957
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• 2007

A new synthetic strategy based on the acid-base catalyzed sol-gel method was developed for the preparation of a series of mesoporous TiO2 nanoparticles. A key feature of the method involves a gradual change in pH (0.8- 9) during the sol-gel transition, which guarantees easy introduction of mesoporosity without relying on the well-established sonochemical or template approach. In addition, this method leads to the exclusive formation of the anatase phase stable enough to the calcination temperature up to 600 oC. The physicochemical properties of the particles in the series were characterized by various spectroscopic and analytical techniques such as wide-angle XRD, SAXRD, BET surface area, FE-SEM, TEM, FT-IR, TGA, and XPS. The photocatalytic efficiency of these materials was investigated for the oxidation of toluene under UV-irradiation. All but T-ad in the series exhibited high photocatalytic activity pushing the reaction into completion within 3 h. The reaction followed the first order kinetics, and the rate reaches as high as 3.9 × 10?2/min which exceeds the one with the commercially available Degussa P-25 by a factor of 3.2. When comparison is made among the catalysts, the reactivity increases with increase in the calcination temperature which in turn increases the crystallinity of the anatase phase, thus revealing the following rate orders: T-3 < T-4 < T-5 < T-6.

• Journal of Life Science
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• v.16 no.1
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• pp.148-155
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• 2006

The diversity of bacterial populations in rivers flowing through Changwon City, was investigated using quinone profiling. The physicochemical properties such as temperature, pH, dissolved oxygen(DO), dissolved organic carbon (DOC) and biochemical oxygen demand (BOD) were also measured in this study. Ubiquinone (UQ)-8, UQ-9 and UQ-10 were observed in all samples for the sites investigated. UQ-8 was the -predominant quinone species in rivers except for Namch'on downstream, T'owolch'on, and Kaumchongch'on in autumn, while UQ-8 was also found as major quinones in the sample except for Hanamch'on, T'owolch'on, Kaumchongch'on, and Namsanch'on in winter. A higher concentration of DOC in rivers yield high concentration of plastoquinone (PQ-9) in autumn and those of total quinones in winter, respectively. Correlation analysis also indicate that BOD is considered to be a major factor controlling the concentration of PQ in rivers.

• Korean Journal of Pharmacognosy
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• v.52 no.3
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• pp.192-201
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• 2021

Dangguisu-san (DGSS) is widely known traditional herbal medicinal formula in Korea for treatment of traumatic injury by traffic accident, ecchymosis, abdominal distension and anti-thrombosis of blood. This study was conducted to develop the simultaneous analyze method using high performance liquid chromatography (HPLC) and examine the effect of anti-inflammatory activity of DGSS-dry extract (DGSS-DE) and DGSS-mix extract powder (DGSS-MEP). Physicochemical characteristics of DGSS-DE and DGSS-MEP showed that there is no significant difference in pH, titratable acidity, total soluble solid content and browning degree except for color value (L, a, b). 15 functional constituents of DGSS were identified and the correlation coefficient values of DGSS-DE and DGSS-MEP were conformed 0.950. Also, DGSS-DE and DGSS-MEP significantly decreased the secretion of nitric oxide (NO), prostaglandin E₂ (PGE₂), interleukin-1β (IL-1β), interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) through inhibited expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), IL-1β, IL-6, and TNF-α. From these result, DGSS-MEP showed similar chemical composition and anti-inflammatory effect to DGSS-DE. Therefore, DGSS-DE and DGSS-MEP may be useful as potential source of drug to prevent inflammation.

• Journal of Electrochemical Science and Technology
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• v.12 no.1
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• pp.137-145
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• 2021

Among the non-precious metal catalysts, iron-nitrogen doped carbon (Fe-N/C) catalysts have been recognized as the most promising candidates for an alternative to Pt-based catalysts for the oxygen reduction reaction (ORR) under alkaline and acidic conditions. In this study, the nano replication method using mesoporous silica, which features tunable primary particle sizes and shape, is employed to prepare the mesoporous Fe-N/C catalysts with different shapes. Platelet SBA-15, irregular KIT-6, and spherical silica particle (SSP) were selected as a template to generate three different kinds of shapes of the mesoporous Fe-N/C catalyst. Physicochemical properties of mesoporous Fe-N/C catalysts are characterized by using small-angle X-ray diffraction, nitrogen adsorption-desorption isotherms, and scanning electron microscopy images. According to the electrochemical evaluation, there is no morphological preference of mesoporous Fe-N/C catalysts toward the ORR activity with half-cell configuration under alkaline electrolyte. By implementing X-ray photoelectron spectroscopy analysis of Fe and N atoms in the mesoporous Fe-N/C catalysts, it is possible to verify that the activity towards ORR highly depends on the portions of "Fe-N" species in the catalysts regardless of the shape of catalysts. It was suggested that active site distribution in the Fe-N/C is one important factor towards ORR activity.

• Proceedings of the Korea Water Resources Association Conference
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• 2020.06a
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• pp.325-325
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• 2020

Low impact development (LID) is a widely used technology that aims to reduce the peak flow volume and amount of pollutants in stormwater runoff while introducing physicochemical, biological or a combination of both mechanisms in order to improve water quality. This research aimed to determine the effect of hydrologic factors in removing the pollutants on stormwater runoff by an LID facility. Monitored storm events from 2010-2018 were analysed to evaluate the hydraulic and hydrological performance of a small constructed wetland (SCW). Standard methods for the examination water and wastewater were employed to assess the water quality of the collected samples (APHA et al, 1992). Primary hydrologic data were obtained from the Korea Meteorological Administration. The recorded average rainfall intensity and antecedent dry days (ADD) of SCW were 5.26 mm/hr and 7 days respectively. During the highest rainfall event (27 mm/hr), the removal efficiency of SCW for all the pollutants was ranging from 67% to 91%. While on the lowest rainfall event (0.7 mm/hr), the removal efficiency was ranging from -36% to 62%. Rainfall intensity has a significant effect to the removal efficiencies of each facility due to its dilution factor. In addition to that, there was no significant correlation of ADD to the mean concentrations of pollutants. Generally, stormwater runoff contains significant amount of pollutants that can cause harmful effects to the environment if not treated. Also, the component of this LID facility such as pre-treatment zone, media filters and vegetation contributed to the effectivity of the LID facilities in reducing the amounts of pollutants present in stormwater runof.

• Journal of Microbiology and Biotechnology
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• v.34 no.2
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• pp.330-339
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• 2024

Corn cobs were fermented with Aspergillus niger to produce soluble dietary fiber (SDF) of high quality and excellent food safety. In this work, the fermentation process was optimized by single-factor test and response surface methodology (RSM). The optimal fermentation conditions were determined to be a material-liquid ratio of 1:30, an inoculum concentration of 11%, a temperature of 32℃, a time of 6 days, and a shaking speed of 200 r/min. Under these conditions, the SDF yield of corn cob increased from 2.34% to 11.92%, and the ratio of soluble dietary fiber to total dietary fiber (SDF/TDF) reached 19.08%, meeting the requirements for high-quality dietary fiber (SDF/TDF of more than 10%). Scanning electron microscopy (SEM) and Fourier-transformed infrared spectroscopy (FT-IR) analysis revealed that the fermentation effectively degraded part of cellulose and hemicellulose, resulting in the formation of a loose and porous structure. After fermentation the water swelling capacity, water-holding capacity, and oil-holding capacity of the corn cob SDF were significantly improved and the adsorption capacity of glucose, cholesterol, and nitrite ions all increased by more than 20%. Moreover, the total phenolic content increased by 20.96%, which correlated with the higher antioxidant activity of SDF. Overall, the fermentation of corn cobs by A. niger increased the yield and enhanced the functional properties of dietary fiber (DF) as well.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.26 no.1
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• pp.63-75
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• 1993

We identified the benthos collected from intertidal zone in Sacho, Naedong, Oran and Kusong of Haenam peninsula in October, 1990 and May, 1991 and examined the distribution pattern on the bases of the analysis of community structure and the physicochemical analysis of sediment including the grain component. The identified benthos consisted of 141 species and 4,641 individuals. The number of species and individuals of Mollusca, Arthropoda and Annelida occupy more than $85\%$ of the total number. When the intertidal zone were divided into soft area and rocky area the species found in rocky area(114 species) were present in a more diverse way than that in soft area(69 species). The composition of species found in 4 localities turned out to be very similar in soft area while in rocky area the most diverse species were found in Kusong. The seasonal appearance frequency of species and individuals showed that the number collected in the spring(106 species, 3,002 individuals) was higher than that in the fall(85 species, 1,639 individuals). The most dominant species was Cerithideopsilla djadjariensis belonging to Gastropoda, Mollusca(412 individuals) and the species collected more than 200 individuals were 5 species of Mollusca and 1 species of Arthropoda. The species diversity index of 4 localities was in the order of Kusong, Oran, Naedong and Sacho revealed no significant differences among regions. The dominance index was low in Kusong and was high in Sacho. The index of interstational species similarity in soft sediment was very similar with $0.44{\sim}0.53$ value and the index was quite high in Kusong and Oran. This agrees very well with the similarity of the grain component of sediment. In case of rocky area the index was quite low with 0.27 for Kusong and Sacho situated far away from each other. Thus the present study suggests that the distribution of the species was subjected to the physicochemical environmental factor such as the grain component of sediment and the content of organic materials including the geographical factor.