• Title/Summary/Keyword: phylogenetic analyses

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Genetic Diversity of Orobanche cumana Populations in Serbia

  • Ivanovic, Zarko;Marisavljevic, Dragana;Marinkovic, Radovan;Mitrovic, Petar;Blagojevic, Jovana;Nikolic, Ivan;Pavlovic, Danijela
    • The Plant Pathology Journal
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    • v.37 no.6
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    • pp.512-520
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    • 2021
  • In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

mtDNA Diversity and Phylogenetic Analysis of Korean Native Goats (한국재래염소의 mtDNA 다양성 및 계통유전학적 분석)

  • Kim, Jae-Hwan;Cho, Chang-Yeon;Choi, Seong-Bok;Cho, Young-Moo;Yeon, Seung-Hum;Yang, Boh-Suk
    • Journal of Life Science
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    • v.21 no.9
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    • pp.1329-1335
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    • 2011
  • Korean native goats, which are characterized by black coat color, have existed on the Korean peninsula for a long time. Until now, there has been no comprehensive investigation concerning their genetic diversity, phylogenetic analysis or origin. In this study, we investigated the genetic diversity and verified phylogenetic status of the Korean native goat using the 453-bp fragment of the hypervariable fragment I (HVI) of mitochondrial DNA (mtDNA) D-loop region from 60 individuals among 5 populations. The Korean native goat showed less haplotype diversity when compared with goats from other countries. In addition, 6 haplotypes that had not been previously reported were verified in this study. In phylogenetic analyses with other country's goats, 10 haplotypes from Korean native goats were classified into mtDNA lineage A. Moreover, in a phylogenetic tree for goats which contained mtDNA lineage A, 8 of 10 haplotypes could be included in a subgroup with goats from Vietnam and an area of China. However, none of the remaining haplotypes belonged to a major group of Korean native goats and were located on different independent positions. These results suggest that almost Korean native goats aligned more closely to China and Vietnam breeds in mtDNA lineage A and there was no gene flow from other mtDNA lineages. Our results will contribute to conservation strategies and genetic breeding of Korean native goats.

Searching biologically plausible synteny blocks among multiple genomes

  • Hachiya, Tsuyoshi;Sakakibara, Yasubumi
    • Proceedings of the Korean Society for Bioinformatics Conference
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    • 2005.09a
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    • pp.113-117
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    • 2005
  • In comparative genome analyses, synteny blocks play important roles for finding ortholog genes, reconstructing phylogenetic tree and predicting genome rearrangement events. In this paper, we propose a novel method to search biologically plausible synteny blocks not only from the viewpoint of finding highly preserved regions but also from the viewpoint of analyzing genome rearrangements. We have applied the method to our experiments on four fungal organisms, and succeeded to obtain some biologically interesting results.

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Pinnal dermatitis caused by Arthroderma vanbreuseghemii in a Rex rabbit

  • Rhim, Haerin;Na, Ki-Jeong;Han, Jae-Ik
    • Korean Journal of Veterinary Service
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    • v.40 no.1
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    • pp.75-78
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    • 2017
  • This report describes a case of pinnal fungal dermatitis caused by Arthroderma vanbreuseghemii in a Rex rabbit diagnosed by clinical signs, macro- and microscopic examination, molecular and phylogenetic analyses. This is the first description of natural A. vanbreuseghemii infection in rabbits in South Korea.

Two Corbicula (Corbiculidae: Bivalvia) mitochondrial lineages are widely distributed in Asian freshwater environment

  • Park, Joong-Ki;Kim, Won
    • Proceedings of the Korean Society of Fisheries Technology Conference
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    • 2003.05a
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    • pp.377-377
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    • 2003
  • We investigated the biogeography of Asian Corbicula using mitochondrial gene sequence variation for Corbicula members sampled from 24 localities of 8 Asian regions. A total of 210 individuals were genetically characterized by examining sequence variations of a 614 bp fragment of the mitochondrial cytochrome oxidase I (COI) gene. Phylogenetic analyses of the COI dataset revealed that Corbicula members are subdivided into two well-supported clades: estuarine and freshwater. (omitted)

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Molecular Identification of Mycorrhizae of Cymbidium kanran (Orchidaceae) on Jeju Island, Korea

  • Hong, Ji Won;Suh, Hyoungmin;Kim, Oh Hong;Lee, Nam Sook
    • Mycobiology
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    • v.43 no.4
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    • pp.475-480
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    • 2015
  • A fungal internal transcribed spacer region was used to identify the mycorrhizae of Cymbidium kanran. The family Russulaceae was found to be the most frequently occurring group in both root and soil samples. In phylogenetic analyses, the majority of the Russulaceae clones were clustered with Russula brevipes and R. cyanoxantha. Therefore, C. kanran may form symbiotic relationships with the genus Russula.

Bremia lactucae causing Downy Mildew on Lactuca serriola in Korea

  • Lee, Jin A;Kim, Bora;Lee, Dong-Jae;Choi, Young-Joon
    • The Korean Journal of Mycology
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    • v.49 no.3
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    • pp.379-383
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    • 2021
  • Lactuca serriola L. (syn. L. scariola L.), commonly known as prickly lettuce, invaded Korea in the late 1970s. The plant has since become widely naturalized and disruptive to native plant communities. In May 2020, downy mildew infections were observed on L. serriola in Gimje-si, Korea. Molecular phylogenetic and morphological analyses identified the causal agent as Bremia lactucae. This is the first report of B. lactucae infection on L. serriola in Korea.

Candelaria asiatica, an Ignored New Species from South Korea

  • Liu, Dong;Hur, Jae-Seoun
    • Mycobiology
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    • v.46 no.4
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    • pp.305-310
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    • 2018
  • The genus Candelaria is characterized by a micro-foliose to micro-fruticose thallus and contains eight species, two of which were reported in South Korea. During the excursion of a Korean lichen flora investigation, some suspected Candelaria concolor specimens were collected, and their morphological, chemical, molecular phylogenetic, and geographic analyses were conducted. The samples eventually proved to be a new species, Candelaria asiatica, which can be recognized by a small, yellow lobate thallus with a pulverulent surface, and a fragile lobe margin with blastidia or phyllidia-like lobules.

Peronospora bulbocapni, an Unreported Species Causing Downy Mildew on Corydalis ambigua in Korea

  • Choi, Young-Joon
    • The Korean Journal of Mycology
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    • v.46 no.4
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    • pp.505-510
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    • 2018
  • Peronospora is the largest genus of the order Peronosporales (Oomycota) and contains more than 550 accepted species, which causes downy mildew on many economically important crops. During a survey of downy mildew flora in Korea, a previously unreported species of Peronospora has been found on Corydalis ambigua. Based on molecular phylogenetic and morphological analyses, the causal agent was identified as Peronospora bulbocapni. This is the first report of Peronospora bulbocapni occurring on Corydalis ambigua in Korea.