• The Korean Journal of Mycology
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• v.48 no.3
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• pp.297-312
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• 2020

Wilted soybean plants were collected from soybeans cultivation fields in Korea from 2014 to 2016. Fusarium spp., Colletotrichum spp., Rhizoctonia spp., Macrophomina sp., Phytophthora spp., and Calonectria ilicicola were obtained from the infected samples. Out of these, Fusarium spp. were the dominant species (79.1%). In total, 53 isolates were identified as F. solani species complex, F. oxysporum species complex, F. graminearum species complex, and F. fujikuroi species complex based on mycological characteristics. Sequence typing analysis was conducted using translation elongation factor 1 alpha (TEF) to confirm the identification of isolates. All isolates were identified as F. solani, F. oxysporum, F. commune, F. asiaticum, and F. fujikuroi based on phylogenetic analysis of TEF sequences. Pathogenicity of 44 isolates was tested on three cultivars of soybean using the root dip inoculation method. Out of 5 Fusarium species, only F. asiaticum could not cause the symptoms or be weak. Ten isolates were selected based on pathogenic characters and species identification to investigate the host range and screen soybean cultivars for resistance. Fusarium solani, F. oxysporum, and F. commune were aggressive only to soybean, and F. fujikuroi was aggressive to kidney bean, yellow cowpea, black cowpea, adzuki bean as well as soybean. All 13 Korean soybean cultivars were susceptible to F. commune and F. fujikuroi. Out of 13 cultivars, cv. Janggi, cv. Poongsannamul, and cv. Socheongja were resistant to Fusarium wilt, while cv. Hwanggeumol and Chamol were susceptible to Fusarium wilt.

• Journal of Ginseng Research
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• v.44 no.1
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• pp.135-144
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• 2020

Background: Panax species are important herbal medicinal plants in the Araliaceae family. Recently, we reported the complete chloroplast genomes and 45S nuclear ribosomal DNA sequences from seven Panax species, two (P. quinquefolius and P. trifolius) from North America and five (P. ginseng, P. notoginseng, P. japonicus, P. vietnamensis, and P. stipuleanatus) from Asia. Methods: We conducted phylogenetic analysis of these chloroplast sequences with 12 other Araliaceae species and comprehensive comparative analysis among the seven Panax whole chloroplast genomes. Results: We identified 1,128 single nucleotide polymorphisms (SNP) in coding gene sequences, distributed among 72 of the 79 protein-coding genes in the chloroplast genomes of the seven Panax species. The other seven genes (including psaJ, psbN, rpl23, psbF, psbL, rps18, and rps7) were identical among the Panax species. We also discovered that 12 large chloroplast genome fragments were transferred into the mitochondrial genome based on sharing of more than 90% sequence similarity. The total size of transferred fragments was 60,331 bp, corresponding to approximately 38.6% of chloroplast genome. We developed 18 SNP markers from the chloroplast genic coding sequence regions that were not similar to regions in the mitochondrial genome. These markers included two or three species-specific markers for each species and can be used to authenticate all the seven Panax species from the others. Conclusion: The comparative analysis of chloroplast genomes from seven Panax species elucidated their genetic diversity and evolutionary relationships, and 18 species-specific markers were able to discriminate among these species, thereby furthering efforts to protect the ginseng industry from economically motivated adulteration.

• The Journal of Natural Sciences
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• v.6 no.1
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• pp.5-39
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• 1993

The Blue-green algae collected from whole coasts of Korea were investigated morphotaxonomically in order to list up Korean marine Cyanophyta and clarify their taxnomic position. As a result, 36 species, 20 genus, 6 families belonging to 3 orders were identified. Among these, 14 species were recorded for the first time in Korea. They are Chroococcus minutus (K$\"{u}$tzing) N$\"{a}$geli, Merismopedia punctata Meyen, Microcystis ichtyoblabe K$\"{u}$zing, Dermocarpa leibleiniae (Reinsch) Born. et Thur., Hydrocoleum confluens (Setchell et Gardner) Drouet, Lyngbya sordida (Zanard.) Gomont, Phormidium forveolarum (Mont.) Gomont, Phormidium hansgieri Schmidle, Skujaella hildebrandtii (Gomont.) de Toni, Sphaeronema lithophila (Ercegovic) Umezaki, Spirulina tenerrima K$\"{u}$tzing, Hormothamnion enteromorphoides Grunow, Michrochaete aeruginea Batters, Michrochaete grisea Thuret ex Born. et flah.. Using the phase contrast microscope and the Nomarski interference micrope, we made photomicrographs of minute blue green algae. The cellular inclusions especially PHB(poly-$\SS$-hydroxy-butyrate) granules of the blue-green algae identified were investigated. The species clearly characteriged to have PHB granule were Lyngbya confervoides, L. semiplena, Phormidium corium, Sirocoleum kurzii, Hormothamnion enteromorphoides and Calothrix crustacea. These result would be fundamental data for estabilishing phylogenetic system of blue-green algae based on physio-biochemical characteristics in future.

• Journal of Life Science
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• v.22 no.10
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• pp.1384-1391
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• 2012

Five coastal plant species, Artemisia fukudo, Aster sphathulifolius, Plantago camtschatica, Sedum oryzifolium, and Setaria viridis, were collected from the coastal region of Ulleung Island (Ulleung-Do, South Korea). Thirty-six endophytic fungi were isolated from the roots of these plants, and all were identified by using PCR with the following specifications: internal transcribed spacer 1 (ITS1), 5.8S rRNA, and ITS2 regions. Phylogenetic analysis indicated that all fungal strains belong to the phylum Ascomycota and comprise four orders (Capnodiales, Eurotiales, Hypocreales, and Pleosporales). Among all the identified species, the Eurotiales species were more abundant than species in the other orders. Nine different genera (Alternaria, Aspergillus, Cladosporium, Exserohilum, Fusarium, Neosartorya, Penicillium, Phoma, and Pyrenochaeta) in the four orders were confirmed. Penicillium and Aspergillus species were the most dominant species among the endophytic fungi isolated from the coastal plants. Shannon's diversity index (H') ranged from 0.684 to 1.609, and the endophytic fungi in S. oryzifolium was more diverse compared to the endophytic fungi in the other plants.

• The Korean Journal of Mycology
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• v.33 no.1
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• pp.1-10
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• 2005

Nineteen strains of Trametes species were collected from the area of Kangwon Province, Korea. They have a variety of color hands and line-up markings on fruit bodies. Most strains were categorized into four types based on color bands, that is, dark brown, light brown, dark gray and light gray. They also have line-up marking shapes from sparse to compact on fruit bodies. In this study, we tried to investigate the relationship between the genetic variation and morphological appearance of these species using the nuclear ribosomal ITS1-5.8S-ITS2 region sequence, we used nineteen strains collected in nature and four species of five standard strains (T. versicolor KCTC16781, KCTC26203, T. villosa KCTC06866, T. suaveolens KCTC26205 and T. hirusta KCTC26200). The data of ITS sequences indicated that nineteen strains of T. versicolor have the difference of $1{\sim}6$ base pairs, comparing with standard strains of T. versicolor KCTC16781, and KCTC26203. Phylogenetic analysis of the Trametes species showed that they grouped into a wide range of single clade. Standard strains except T. versicolor KCTC16781 and KCTC26203, formed separated subgroup.

• Korean Journal of Medicinal Crop Science
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• v.13 no.6
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• pp.278-286
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• 2005

This study was conducted to classify 80 Artemisia spp. collections based on morphological characters and RAPD analysis to get the basic information of Artemisia spp. collections as medicinal plants. The eighty Artemisia spp. collections were classified into 5 groups with the maximum distance 0.82 between clusters based on the complete linkage cluster analysis with morphological traits. Out of 80 operon primer, 10 primers showing polymorphic bands were selected for RAPD analysis. Among the 98 bands amplified with the primers, 68 (69%) bands showed polymorphism. The number of amplified bands ranged from 8 to 10 with an average number of 9.8 bands. Artemisia spp. collections classified into 6 groups with the similarity value of 0.63 in dendrogram derived from the cluster analysis based on RAPDs. Group consisted of 29 collections. Group, which is the largest one, contained 40 collections. Most of the A. asiatica and A. feddei LEV et VNT. were classified into Group and. The rest of the collections (31%) were classified into Group $III{\sim}V$.

• Korean Journal of Medicinal Crop Science
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• v.16 no.4
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• pp.218-224
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• 2008

This study was conducted to obtain the basic data for using the Artemisia genetic resources as a medicinal crop. 24 taxa including Artemisia capillaris Thunb. were analyzed by principal component analysis of 25 characters and cluster analysis for classification. In Principal components analysis of individuals of taxa using 25 morphological characters of reproductive organ, the first, the second, the third and the fourth components contributed 44.73%, 16.86%, 8.88%, and 7.07% of the variations, respectively. The cumulative contribution from the first to the fourth principal components was 77.56%. In cluster analysis, taxa of Artemisia L. was seperated 3 group by 25 morphological characters of reproductive organ, but it didn't completely coincident with Kitamura classification.

• Korean Journal of Microbiology
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• v.33 no.2
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• pp.149-156
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• 1997

We have employed comparative RFL,P(Restriction Fragment Ixngth Pol~iniorphism) analysis and molecular phylogenetic techniques to investigate the diversity of uncultured microorganisms associated with the anaerobic PCP degradation in PCP-adapted enrichment cultures inoculated by samples from anaerobic cewage sludgc(Jangrim, Pusan) and leachate of landfill site(Kimhae). 16s rDNA cloncs were obtairted by PCR amplification of mixed population DNAs extracted directly from the nonactive and active stage ol each PCP-adapted culture. After three rounds of comparative RFLP analyses. two RFLP types. designated as Ala and Hld, were found prevalent and common in both active stage samples. Thc analysis of phylogenctic diversity bawd on the 5'-terminal 180 nt of sequences from whole clones of the Ala and Bld RFLP types showed close similarity among themselves. In case of Bld clones, 7XQ of them shared identical sequences. Thcse resuliq suggest that the clones of both RFLP types wcre originated from highly affiliated microorganisms which are e~iriched as a result of metabolic activity to PCP. The full-length 16s rRNA sequence of each representative clone from both RFLP types was determined. and an Ala clone w i n found to he related to Clo.strrdiurn ulfutzac~(Genk~ank No. Z69203) and a Bld clone to Thermobacteroides proteolyticus(Genbank No. X09335), with sequence similarities of 89%' and 97%. respectively.

• Korean Journal of Microbiology
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• v.45 no.4
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• pp.368-376
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• 2009

A red pigment-producing bacterial strain was isolated from sediment sample of the East China Sea. The isolate was identified by analysis based on 16S rDNA sequence and morphological, physiological properties, biochemical characteristics and fatty acid composition. Phylogenetic analysis based on 16S rDNA sequence showed that isolate represent a phyletic lineage within the genus Zooshikella, and this strain was most closely related to Zooshikella ganghwensis KCTC $12044^T$ (AY130994) (99.79%). The strain was Gram-negative, aerobic and required NaCl at 0.5~8.0% for growth. The predominant cellular fatty acids were saturated and monounsaturated straight-chain fatty acids. Consequently, this strain was identified as a member of the genus Zooshikella and designated as Zooshikella sp. JE-34. The pigment showed characteristics similar to prodigiosin, a well-known red pigment previously detected in Serratia marcescens. The antimicrobial activity of Zooshikella sp. JE-34 bacterial pigment was tested against 18 microorganisms, which were fish and human pathogens. The Zooshikella sp. JE-34 red pigment showed high antimicrobial activity against Streptococcus iniae, S. parauberis, S. mutans, Staphylococcus aureus, and Propionibacterium acnes.

• Korean Journal of Microbiology
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• v.53 no.1
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• pp.9-19
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• 2017

We studied soil composition, $N_2O$ production, a number of denitrifying bacteria, community structure and T-RFLP patterns of denitrifying bacteria dependent on agricultural methods with the change of seasons. Analyses of the soil chemical composition revealed that total carbon and total organic carbon contents were 1.57% and 1.28% in the organic farming soil, 1.52% and 1.24% in the emptiness farming soil, and 1.40% and 0.95% in traditional farming soil, respectively. So, the amount of organic carbon was relatively high in the environment friendly farming soils than traditional farming soils. In case of $N_2O$ production, the amount of $N_2O$ production was high in May and November soils, but the rate of $N_2O$ production was fast in August soil. The average number of denitrifying bacteria were $1.32{\times}10^4MPN{\cdot}g^{-1}$ in the organic farming soil, $1.17{\times}10^4MPN{\cdot}g^{-1}$ in the emptiness farming soil, and $6.29{\times}10^3MPN{\cdot}g^{-1}$ in the traditional farming soil. It was confirmed that the environment friendly farming soil have a larger number of denitrifying bacteria than the traditional farming soil. As a result of the phylogenetic analyses, it was confirmed that six clusters were included in organic farming soil among total 10 clusters. And the result of PCA profile distribution of T-RFLP pattern on agricultural methods, the range of distribution showed wide in the organic farming method, relatively narrow in the conventional farming method, and middle in the emptiness farming method. Therefore, we could concluded that the distribution and the community structure of denitrifying bacteria were changed according to the agricultural methods and seasons.