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http://dx.doi.org/10.5352/JLS.2012.22.10.1384

Genetic Diversity of Endophytic Fungal Strains Isolated from the Roots of Coastal Plants in Ulleung Island for Restoration of Coastal Ecosystem  

Kim, Miae (School of Life Sciences and Biotechnology, Kyungpook National University)
You, Young-Hyun (School of Life Sciences and Biotechnology, Kyungpook National University)
Yoon, Hyeokjun (School of Life Sciences and Biotechnology, Kyungpook National University)
Kim, Hyun (School of Life Sciences and Biotechnology, Kyungpook National University)
Seo, Yeonggyo (School of Life Sciences and Biotechnology, Kyungpook National University)
Khalmuratova, Irina (School of Life Sciences and Biotechnology, Kyungpook National University)
Shin, Jae-Ho (School of Applied Biosciences, Kyungpook National University)
Lee, In-Jung (School of Applied Biosciences, Kyungpook National University)
Choo, Yeon-Sik (Department of Biology, College of National Sciences, Kyungpook National University)
Kim, Jong-Guk (School of Life Sciences and Biotechnology, Kyungpook National University)
Publication Information
Journal of Life Science / v.22, no.10, 2012 , pp. 1384-1391 More about this Journal
Abstract
Five coastal plant species, Artemisia fukudo, Aster sphathulifolius, Plantago camtschatica, Sedum oryzifolium, and Setaria viridis, were collected from the coastal region of Ulleung Island (Ulleung-Do, South Korea). Thirty-six endophytic fungi were isolated from the roots of these plants, and all were identified by using PCR with the following specifications: internal transcribed spacer 1 (ITS1), 5.8S rRNA, and ITS2 regions. Phylogenetic analysis indicated that all fungal strains belong to the phylum Ascomycota and comprise four orders (Capnodiales, Eurotiales, Hypocreales, and Pleosporales). Among all the identified species, the Eurotiales species were more abundant than species in the other orders. Nine different genera (Alternaria, Aspergillus, Cladosporium, Exserohilum, Fusarium, Neosartorya, Penicillium, Phoma, and Pyrenochaeta) in the four orders were confirmed. Penicillium and Aspergillus species were the most dominant species among the endophytic fungi isolated from the coastal plants. Shannon's diversity index (H') ranged from 0.684 to 1.609, and the endophytic fungi in S. oryzifolium was more diverse compared to the endophytic fungi in the other plants.
Keywords
Endophytic fungi; genetic diversity; coastal plant; Ulleung island;
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