• Journal of Environmental Science International
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• v.1 no.1
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• pp.51-59
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• 1992

Two comparative poplar clones (I-214: Populus euramerinm, Peace: P koreana x p. trihocarpa) were exposed to two $CO_2$ concentrations (350 or 2, 000 ${\mu}L L^{-1} CO_2$) for 21 days. When both poplar clones were compared at growth conditions, the net photosynthetic rate ($P_N$) in $CO_2$-enriched ($2, 000{\mu}L L^{-1} CO_2 = C_{2, 000}$) plants become about 50-60% higher than that of 350 ${\mu}L L^{-1} CO_2 (=C_{350}$ Plants on 7 days treatment. But the enhancement of PN by high $CO_2$ was not maintained throughout all the experimental period. At 21 days, there was no difference of photosynthetic rates between $C_{350}$ and $C_{2000}$ plants. In contrast with photosynthesis, the response of leaf conductance to the elevated $CO_2$ concentration was very different between I-214 and Peace. During all experimental period, leaf conductance ($g_{s}$) of $C_{2000}$ plants is 50% lower than that of the $C_{350}$ plants for I-214, while there is no difference of gs between the plants of $C_{350}$ and $C_{2, 000}$ for Peace. The results of gs in Peace indicate that decreased photosynthetic rate after 21 days in $C_{2, 000}$ Plants for two poplar clones is possibly due to non-stomatal factors. To investigate the non-stomatal factors, starch accumulation and ribulose-1, 6-bisphosphate carboxylase (RuBPCase) were measured. We found significant accumulation of starch in two poplar clones exposed to high $CO_2$, especially starch of I-214 in $C_{2, 000}$ become 3.5 times higher than in $C_{350}$ plants at 21 days. This suggests that high proportion of photosynthates was directed into starch. After 21 days, the activity of ribulose-1, 6-bisphosphate carboxylase of $C_{2, 000}$ plants become decreased in 40-50% compared with that of the $C_{350}$ plants. Two poplar clones show the same trend to RuBPCase declines under high $CO_2$ concentration, although the decline is more significant for I-214. The results reported here suggest that starch accumulation and decreased RuBPCase activity in $C_{2, 000}$ plants can be partly ascribed to the loss of photosynthetic efficiency of high $CO_2$-grown poplar plants.

• Korean Journal of Environmental Biology
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• v.40 no.3
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• pp.352-362
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• 2022

Photosynthesis and respiration of seagrasses are mainly controlled by water temperature. In this study, the photosynthetic physiology and respiratory changes of the Asian surfgrass Phyllospadix japonicus, which is mainly distributed on the eastern and southern coasts of Korea, were investigated in response to changing water temperature (5, 10, 15, 20, 25, and 30℃) by conducting mesocosm experiments. Photosynthetic parameters (maximum photosynthetic rate, P_max; compensation irradiance, I_c; and saturation irradiance, I_k) and respiration rate of surfgrass increased with rising water temperature, whereas photosynthetic efficiency (α) was fairly constant among the water temperature conditions. The P_max and I_k dramatically decreased under the highest water temperature condition (30℃), whereas the I_c and respiration rate increased continuously with the increasing water temperature. Ratios of maximum photosynthetic rates to respiration rates (P_max : R) were highest at 5℃ and declined markedly at higher temperatures with the lowest ratio at 30℃. The minimum requirement of H_sat (the daily period of irradiance-saturated photosynthesis) of P. japonicus was 2.5 hours at 5℃ and 10.6 hours at 30℃ for the positive carbon balance. Because longer H_sat was required for the positive carbon balance of P. japonicus under the increased water temperature, the rising water temperature should have negatively affected the growth, distribution, and survival of P. japonicus on the coast of Korea. Since the temperature in the temperate coastal waters is rising gradually due to global warming, the results of this study could provide insights into surfgrass responses to future severe sea warming and light attenuation.

• Journal of Korean Society of Forest Science
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• v.113 no.2
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• pp.198-209
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• 2024

This study aimed to investigate the impact of light intensity, manipulated through different shading levels, on the growth and physiological responses of Thermopsis lupinoides. To assess the effects of shading treatments, we examined leaf mass per area, chlorophyll content, chlorophyll fluorescence response, and photosynthetic characteristics. T. lupinoidesexhibited adaptive responses under low light conditions (50% shading), showing increased leaf area and decreased leaf mass per area as shading levels increased. These changes indicate morpho-physiological adaptations to reduced light availability. At 50% shading, the physiological and ecological responses were favorable, with optimal photosynthetic functions including chlorophyll content, photosynthesis saturation point, photosynthetic rate, carbon fixation efficiency, stomatal conductance, transpiration rate, and water use efficiency. However, at 95% shading, the essential light conditions for growth were not met, significantly impairing photosynthetic functions. Consequently, 50% shading was determined to be the most optimal condition for T. lupinoides growth. These findings provide valuable insights for effective ex-situconservation practices and site selection for T. lupinoides, serving as foundational data for habitat restoration efforts.

• Korean Journal of Environmental Biology
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• v.30 no.3
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• pp.210-218
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• 2012

The morphological characteristics, carbon and nitrogen concentrations, stable isotope values and photosynthetic rates of Porphyra yezoensis were studied at the main purple lavers production areas on southwestern coast of Korea. The morphological characteristics of leaf length, leaf width and weight of Porphyra blades were between 11.6~16.3 (average 13.8) cm, 4.6~6.3 (average 5.4) cm, $1.1{\sim}2.6(average\;1.86)g\;DW\;m^{-2}$, respectively. Photosynthetic pigment of Chl a concentration of Porphyra blades was between $2.18{\sim}17.77(average\;9.65)mg\;DW\;Chl\;a\;m^{-2}$. Carbon and nitrogen concentrations of Porphyra blades was between $201{\sim}317(average\;240)mg\;DW\;g^{-1}$, $39.8{\sim}50.0(average\;43.5)mg\;DW\;g^{-1}$ and C/N ratio 5.0~6.7 (average 5.5). The range of average ${\delta}^{13}C$ and ${\delta}^{15}N$ values of Porphyra blades was between - 25.6 to - 24.0 (average - 24.7)‰ for ${\delta}^{13}C$, and 1.3 to 4.1 (average 2.1)‰ for ${\delta}^{15}N$. Photosynthetic characteristics of seaweeds measured by pulse amplitude modulation (PAM) fluorometry was used as an indicator of photosynthetic activity. We use Diving-PAM fluorometry to examine photosynthetic rates of the seaweeds Porphyra yezoensis at each station. Maximum quantum yield of Porphyra blades was between 0.46~0.55 (average 0.52), the variance of the effective PS II maximum quantum yield of the station was broadly similar. Maximum relative electron transport rate (rETRmax) of Porphyra blades was between $4.71{\sim}5.84(average\;5.33){\mu}mol\;electrons\;m^{-2}\;s^{-1}$, the changes of maximum relative electron transport rate (rETRmax) of Porphyra yezoensis were similar to those of PS II maximum quantum yield. Photosynthetic efficiency (${\alpha}$) was between 0.027~0.045 (average 0.036). Minimum saturating irradiance ($E_k$) range was $139{\sim}180(average\;156){\mu}mol\;photons\;m^{-2}\;s^{-1}$. Minimum saturating irradiance ($E_k$) made a difference by station within the area on southwestern coast. Carbon and nitrogen concentrations and photosynthetic rates of Porphyra blades production areas on southwestern coast were broadly similar. The photosynthetic characteristics showed low photosynthetic rates because the low maximum quantum yields and low maximum relative electron transport rate.

• Journal of Korean Society of Forest Science
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• v.111 no.3
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• pp.405-417
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• 2022

The purpose of this study was to describe the photosynthetic features of Prunus maximowiczii and Prunus serrulate Lindl. var. pubescens (Makino) Nakai in response to drought stress. Specifically, we studied the effects of drought on photosynthetic ability and photosystem II activity. Drought stress (DS) was induced by cutting the water supply for 30 days. DS decreased the moisture contents in the soil, and between the 10th and 12th days of DS, both species had 10% or less of x., After the 15th day of DS, it was less than 5%, which is a condition for disease to start. We observed a remarkable decrease of maximum photosynthesis rate starting from 10th day of DS; the light compensation point was also remarkable. Dark respiration and net apparent quantum yield decreased significantly on the 15th day of DS, and then increased on the 20th day. In addition, the stomatal transpiration rate of P. maximowiczii decreased significantly on the15th day of DS, and then increased on the 20th day. Water use efficiency increased on the 15th day of DS, and then decreased on the 20th day. The stomatal transpiration rate of P. serrulate decreased significantly on the 20th day of DS, and then increased afterward, while its water use efficiency increased on the 20th day of DS, and then decreased afterward. These results indicate that the closure of stoma prevented water loss, resulting in a temporary increase of water use efficiency. Chlorophyll fluorescence analysis detected remarkable decreases in the functional index (PI_ABS) and energy transfer efficiency in P. maximowiczii after the 15th day of DS. Meanwhile, photosystem II activity decreased in P. serrulate after 20 days of DS. In addition, Ts-Ta, PI_ABS, DI_O/RC, ET_O/RC followed similar trends as those of the soil moisture content and photosynthetic properties, indicating that they can be used as useful variables in predicting DS in trees.

• Journal of Plant Biotechnology
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• v.30 no.4
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• pp.399-403
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• 2003

In order to understand the protection effects of antioxidant enzymes against oxidative stress caused by various environmental stresses, transgenic tobacco (Nicotiana tabacum cv, Xanthi) plants expressing both copper/zinc superoxide dismutase (CuZnSOD) and ascorbate peroxidase (APX) in chloroplasts (referred to as CA plants) were subjected to highlight (1,100$\mu$mol m$^{-2}$ sec$^{-1}$) and chilling at 4$^{\circ}C$. The protection effects of CA plants using leaf discs were compared with those of transgenic plants expressing either CuZnSOD or APX in chloroplasts (SOD plants or APX plants, respectively) and non-transgenic (NT) plants. CA plants showed about 15％ protection in the photosynthetic efficiency (Fv/Fm) of photosystem II relative to NT plants 1 hr after treatment of both highlight and chilling, whereas they showed about 23％ protection in the redox state of P700 in photosystem I at 3 hr after treatment. SOD plants or APX plants showed an intermediate protection effect between CA plants and NT plants. These results demonstrated that the coexpression of CuZnSOD and APX in chloroplasts importantly involves in the protection effects against oxidative stress caused by various environmental stresses.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.64 no.1
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• pp.48-54
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• 2019

This research was carried out to investigate the plant growth, inorganic ion and amino acid content characteristics in a halophyte, Suaeda glauca, under different NaCl concentrations for cultivating in the reclaimed land. S. glauca was hydroponically cultivated under 0, 50, 100, 200, 300 and 400 mM NaCl concentrations with Hogland's nutrient solution. To evaluate growth response under different NaCl concentrations, plant height and number of branches, dry weight, Fv/Fm value, and photosynthetic efficiency were investigated. To find out physiological characteristic, inorganic ion contents and amino acids in the plant were evaluated. The optimum concentration of NaCl for plant growth were 50 mM. The plant growth were gradually decreased in the concentration ranged from 100 to 400 mM. As increasing of NaCl concentration, Na ion was increased, but K, Ca, Mg ions were decreased in the plant. The amino acid contents were varied due to NaCl concentrations, but most of amino acids content in total plant was the lowest at 50 mM. Conversely, proline was exceptionally high at 50 mM of NaCl concentration. The Fv/Fm value was the highest at 50 mM of NaCl concentration. From these results, the optimum salt concentration for the growth of S. glauca was 50 mM, but the plant seems to adapt in a variety of salt environments in view of the change of ions and amino acids depending on salt concentration and the maintenance of photosynthetic efficiency even under high salt condition.

• Journal of Bio-Environment Control
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• v.27 no.2
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• pp.132-139
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• 2018

The photosynthetic rate is an indicator of the growth state and growth rate of crops and is an important factor in constructing efficient production systems. The objective of this study was to develop a canopy photosynthetic rate model of romaine lettuce using the three variables of $CO_2$ concentration, light intensity, and growth stage. The canopy photosynthetic rates of the lettuce were measured at five different $CO_2$ concentrations ($600-2,200{\mu}mol{\cdot}mol^{-1}$), five light intensities ($60-340{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$), and four growth stages (5-20 days after transplanting) in three closed acrylic chambers ($1.0{\times}0.8{\times}0.5m$). A simple multiplication model expressed by multiplying three single-variable models and the modified rectangular hyperbola model including photochemical efficiency, carboxylation conductance, and dark respiration, which vary with growth stage, were also considered. In validation, the $R^2$ value was 0.923 in the simple multiplication model, while it was 0.941 in the modified rectangular hyperbola model. The modified rectangular hyperbola model appeared to be more appropriate than the simple multiplication model in expressing canopy photosynthetic rates. The model developed in this study will contribute to the determination of an optimal $CO_2$ concentration and light intensity with the growth stage of lettuce in plant factories.

• Journal of Bio-Environment Control
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• v.16 no.3
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• pp.217-221
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• 2007

To provide the basic data essential for developing varieties of broad-leaved tree, the study investigated the utmost growth requirement for the growth characteristic and biomass production in accordance with planting density of Styrax obassia. The planting density of seedling can be a significant factor in contributing growth of seedling for tree growing in general. While growth of tree according to $1m^2$ planting density of Styrax obassia showed an excellent growth in tree height and the root collar diameter from $49no./m^2$, those low planting density showed decreased tendency with tree height growth while increasing the root collar diameter growth. As similar to the growth characteristic, the biomass production showed significant and statistic difference in $49no./m^2$, showing high volume of biomass production which is $3.12{\pm}0.80g$ compare to other processing section and high figure with T/R ratio which is 1.59. The special trait of photosynthetic also showed relatively high photosynthetic rate in $49no./m^2\;and\;64no./m^2$ of Styrax obassia and as the density increase, photosynthetic efficiency decreased. The plant showed stable and physiological planting pattern, displaying the best photosynthetic rate, which was the final metabolism through reserving proper space in the growth and development environment condition. This obstacle of required space essential for growth substantially deteriorated planting and ultimately, it demonstrated lower tendency of photosynthetic rate, which is the highest level of metabolism.

• Korean Journal of Medicinal Crop Science
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• v.28 no.1
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• pp.1-8
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• 2020

Background: This study was conducted to investigate the effects of NaCl concentration on the photosynthetic parameters, chlorophyll fluorescence and growth characteristics of Crepidiastrum sonchifolium. Methods and Results: As treatments, we subjected C. sonchifolium plants to four different concentrations of NaCl (0, 50, 100 and 200 mM). We found that the photosynthetic parameters maximum photosynthesis rate (P_{N max}), net apparent quantum yield (Φ), maximum carboxylation rate (V_cmax), and maximum electron transport rate (J_max) were significantly reduced at an NaCl concentration greater than 100 mM. In contrast, there was an increase in water-use efficiency with increasing NaCl concentration, although in terms of growth performances, leaf dry weight, root dry weight, stem length, and total dry weight all decreased with increasing NaCl concentration. Furthermore, leakage of electrolytes, as a consequence of cell membrane damage, clearly increased in response to an increase in NaCl concentration. Analysis of the polyphasic elevation of chlorophyll a fluorescence transients (OKJIP) revealed marked decrease in flux ratios (Φ_PO, Ψ_O and Φ_EO) and the PI_abs, performance index in response to treatment with 200 mM NaCl, thereby reflectings the relatively reduced state of photosystem II. This increase in fluorescence could be due to a reduction in electron transport beyond Q^-_A. We thus found that the photosynthetic parameters, chlorophyll fluorescence and growth characteristics of C. sonchifolium significantly increased in response to treatment with 200 mM NaCl. Conclusions: Collectively, the findings of this study indicate that C. sonchifolium shows relatively low sensitivity to NaCl stress, although photosynthetic activity was markedly reduced in plants exposed to 200 mM NaCl.