• Journal of Nutrition and Health
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• 제35권5호
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• pp.505-511
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• 2002

The study was designed to compare the anti-carcinogenic effect of different level of conjugated linoleic acid (CLA) in 1,2-dimethylhydrazine (DMH)-treated rats by determining biomarkers (apoptosis, cell proliferation, eicosanoids, 1,2-diacylglycerol) and phospholipid fatty acid profile in colonic mucosa. Eighty male Sprague Dawley rats weighing 180-220g were randomly divided into 4 groups depending on the content of CLA, i.e. 0.0% CLA, 0.5% CLA, 1.0% CLA, 1.5% CLA. The experimental diet contained protein 21.6%, carbohydrate 54.6%, and fat 14.5% including CLA mixture at different level by weight. The experimental diet was fed for 14 weeks with the initiation of intramuscular injection of DMH, which was injected twice a week for 6 weeks to give total amount of 180 mg/kg body weight. Regardless of the amount of CLA supplemented to diet, CLA significantly increased the apoptotic index but did not have significant effect on cell proliferation in colonic mucosa. CLA was undetected in colonic mucosal phospholipid of rats fed the 0% CLA diet and increased to 5.9mg/g phospholipid in rats fed the 0.5% diet. The apoptotic index was increased by 251% and the 1,2-DAG content was decreased by 57% in rats fed 0.5% CLA. No further changes in these variables were observed when CLA in the diet was raised to 1.0% or 1.5%. However, dietary CLA decreased mucosal levels of prostaglandin (PG)E$_2$, thromboxane (TX)B$_2$, and arachidonic acid in dose-dependent manner. The present data indicate that dietary CLA can inhibit DMH-induced colon carcinogenesis by mechanism probably involving increased apoptosis.

• 한국가금학회지
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• 제22권1호
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• pp.31-42
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• 1995

This study was to investigate the effects of dietary linoleic acid(18:2\omega6, LA) and aipha-linolenic acid(18:3\omega3. \alpha-LNA) levels on brain development and fatty acid compositions of various lipid classes in the chicken embryo brain tissues. Thirty two ISA Brown layers, 52 weeks-old, were divided into four groups. Birds of each group were given corn-soybean meal based diets added with 1) safflower oil 8%, 2) safflower oil 6% + perilla oil 2%, 3) safflower oil 2% + perilla oil 6%, or 4) perilla oil 8%. Mter 15 days fed the diets. the layers were artificially inseminated to obtain fertile eggs. During the incubation. embryonic brains were sampled at 15th and 21st days. Fatty acid contents were quantitated by using heptadecanoic acid (17:0) as an internal standard. No significant differences in brain weight and in contents of various lipids such as phospholipid. triglyceride, cholesterol. cholesterol ester and free fatty acid in the tissues were found among the dietary groups (P<0.05). The ratios of AA/LA in the brain lipid classes were lowered as the dietary levels of perilla oil were increased. Higher LA was found in phosphatidylcholine(PC) than arachidonic acid (20:4\omega6. AA), meanwhile the level of LA was less than AA in phosphatidylethanolamine(PE). Docosahexaenoic acid(22:6\omega3, DHA) was the* major fatty acid in the tissue and its content in PE was 2.5~3 times higher than in PC. DHA level in the phospholipid reached at a peak (1.7~1.8 mg/brain) in dietary groups added with 6% or 8% perilla oil. suggesting that no more increase in that fatty acid level in the brain tissue could be obtained by consuming more \alpha-LNA, the major precursor of DHA.

• 대한한방내과학회지
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• 제18권2호
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• pp.195-206
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• 1997

In order to study the effects of Ohwangsahwatang on Hyperchole-sterolemia, experimental study were performed on Hypercholesterolemia Rats induced by 2% cholesterol diet. Also the level of total cholesterol. triglyceride, LDL-cholesterol. HDL-cholesterol, phospholipid, transami-nase(GOT, GPT) were measured. The results were summerized as follows : In the model of Hypercholesterolemia induced by 2% cholesterol diet in rats, Ohwangsahwatang showed decreasing effects on total cholesterol, triglyceride, LDL-cholesterol, phospholipid level, and transaminase (GOT, GPT) activity in serum significantly, depending on the concentration, and showed increasing effects on HDL-cholesterol level in serum significantly depending on the concentration. According to the above results, Ohwangsahwatang showed significant decreasing effects on Hypercholesterolemia, and it is considered that it is appropriate to apply for Hypercholesterolemia.

• Archives of Pharmacal Research
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• 제16권2호
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• pp.147-151
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• 1993

In diabetes, the abnormal increase of the membrane cholesterol/phospholipid ratio (C/PL) is consdered to be the main reason for the decreased membrane fluidity, which then results in impaired erythrocyte deformability and subsequent microcirculatory disturbances. In this study, we examined the effects of brazilin on lipid and phosphatidyl fatty acid composition of erythrocyte membranes in streptozotocin induced diabetic rats. Treatment of brazilin (10mg/kg or 100 mg/kg for 2 weeks, i.p) altered and cholesterol contents in diabetic erythrocyte membranes. The C/PL ratio of brazilin treated groups decreased compared with that of diabetic control group while no change was observed in normal erythrocytes. In streptozotocin induced diabetic rats, alterations in phosphatidyl fatty acid compositioin of erythrocyte membranes were observed and brazilin could reverse these alterations. Arachidonic acid level reumed to a normal level while linoleic acid level remained unchanged by the treatment of brazilin. The results suggest that brazilin might increase erythrocyte membrane fluidity which plays a key role inregulating erythrocyte deformability, thereby it could exert positive effects on microdiculatory disturbances.

• 한방비만학회지
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• 제18권1호
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• pp.27-35
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• 2018

Objectives: The aim of this study is to investigate the effects of Daecheongryoung-tang (DCR) therapy on body weight, serum total cholesterol, high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, triglyceride, free fatty acid, total lipid, phospholipid level and complete blood cell count of obese rats. Methods: 34 rats are divided into 4 groups, the rats in the normal group are 7 and the rats in the other group are 9 per group; Normal group (general fat diet and no medication), Control group (high-fat diet and no medication), DCR_L group (high-fat diet and DCR 250 mg medication) and DCR_H group (high-fat diet and DCR 500 mg medication). DCR is administrated for 6 weeks. Results: There is significant statistical difference between Control group and DCR-H group for the body weight, the total cholesterol, LDL cholesterol, triglyceride, free fatty acid level. Also, there is significant statistical difference among Control group, DCR_L group and DCR_H group for body weight, triglyceride, free fatty acid and phospholipid level. Conclusions: These results suggest that medication of DCR_L and DCR_H is effective for the treatment of obesity.

• 대한약침학회지
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• 제6권1호
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• pp.61-66
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• 2003

In order to study the effects of Moschus Herbal-acupuncture on hyperlipidemia, we divided the rats into three groups(Normal group, Control group & Sample group) and performed the experimental research. Hyperlipidemia rats were induced by Triton WR-1339(150mg/kg) for 3days. Sample group was injected Moschus Herbal-acupuncture 0.1 ml/200g for 3 days and control group was injected equal dose of saline. And then we measured the amount of serum total cholesterol, triglyceride, phospholipid and HDL-cholesterol. The results were as follows : 1.Moschus Herbal-acupuncture showed decreasing effects on total cholesterol, triglyceride and phospholipid levels in serum significantly(p<0.05). 2.Moschus Herbal-acupuncture showed increasing effects on HDL-cholesterol level in serum significantly(p<0.05). According to the above results, Moschus Herbal-acupuncture showed significant decreasing effects on hyperlipidemia and it is considered that it is appropriate to apply for hyperlipidemia.

• 한국식품영양학회지
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• 제9권2호
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• pp.201-212
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• 1996

The purpose of this study was to find an effect of honey on the lipid metabolism of Sprague Dawley rats. All experimental rats were fed ad libitum, for seven weeks, 68% saccharide diet and 10% or 20% honey from acacia, sumac and miscelllaneous flower honey, respectively, and sucrose. The food efficiency ratio of rat taken diet with honey and high fructose of control group was increased in comparison with the control group. The concentration of cholesterol in serum of rats take총 diet with and high fructose of control group was more increased in comparison with the control and normal group. The concentration of H DL-cholesterol in serum of rats taken sumac honey was increased 57.0% in comparison with the control group, but the concentration of VLDL, LDL-cholesterol in serum of rats taken diet 10PA sumac honey was decreased 48.36% in comparison with the control group. The concentration of phospholipid in serum of rats taken diet with 20% acacia or 10% miscellaneous honey was increased 24.7, 16.25%, respectively, in comparison with the control group. The concentration of free fatty acid in serum of rats taken Inlet with sumac or miscellaneous honey and high fructose was increased in comparison with the comparison with the control group. The concentration supplemented diet with acasia honey was increase in comparison with the control group. The concentration of triglyceride in serum of rat was increased by feeding of honey. The concentration of triglyceride in liver was increased, but the level of phospholipid was decreased by feeding of honey.

• 대한한방내과학회지
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• 제19권1호
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• pp.202-218
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• 1998

In order to evaluate the effects of SIMNETONG, GANGJIYOUNSOODAN and CHUNGSIMJOKITANG(Xinnao-Tong, Jiangzhiyanshou-Dan and Qingxintiaoqi-Tang) on hyperlipidemia, the experimental study was performed in rats without or with hyperlipidemia induced by 2% cholesterol fed-diet or Triton WR-1339. And the levels of serum total cholesterol, triglyceride, HDL-cholesterol, LDL-cholesterol and phospholipid were measured. The results are summarized as follows : In the model of hyperlipidemia induced by 2% cholesterol Fed-diet 1. Both low & highly concentrated GANGJIYOUNSOODAN showed significantly decreasing effects on the level of serum total cholesterol while SIMNETONG & CHUNGSIMJOKlTANG didn't. 2. GANGJIYOUNSOODAN, SIMINETONG and CHUNGSIMJOKITANG showed decreasing effects on the level of serum triglyceride with no statistical significance. 3. Only highly concentrated GANGJIYOUNSOODAN showed increasing effects on the level of serum HDL-cholesterol while the others had no effects. 4. Only highly concentrated GANGJIYOUNSOODAN had decreasing effects on the level of serum LDL-cholesterol while the others had no effects. 5. Highly concentrated GANGJIYOUNSOODAN and CHUNGSIMJOKITANG showed significantly decreasing effects on the level of serum phospholipid while the other had no effects. In the model of hyperlipidemia induced by Triton WR-1339 6. SIMNETONG, GANGJIYOUNSOODAN and CHUNGSIMJOKITANG showed slightly decreasing effects on the levels of serum total cholesterol and triglyceride with no statistical significance. 7. None of SIMNETONG, GANGJIYOUNSOODAN and CHUNGSIMJOKITANG showed any effects on the level of serum HDL-cholesterol. 8. Highly concentrated GANGJIYOUNSOODAN and CHUNGSIMJOKITANG showed decreasing effects on the level of serum LDL-cholesterol but the other had no effects. 9. All of highly concentrated SIMNETONG, GANGJIYOUNSOODAN and CHUNGSIMJOKITANG showed decreasing effects on the level of serum phospholipid with statistical significance. These results show that GANGJIYOUNSOODAN and CHUNGSIMJOKITANG, in orders, have antihyperlipidemic effects. Further study on the mechanism and on the herb screening of these prescription should be followed.

• 보험의학회지
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• 제2권1호
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• pp.101-115
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• 1985

In Med Dept. Daehan Education Insurance, Total 690 cases(male 376 cases, female 314 cases) were inspected to study the change of serum lipid value. The objects of investigation were total cholesterol, HDL cholesterol, triglyceride, phospholipid and total lipid value. The results are as follows: 1) In the normal subjects, with the relation of serum lipid value and the age. triglyceride, phospholipid and total lipid value showed the increasing tendency by 70 age, but total cholesterol value and HDL cholesterol value showed the increasing tendency by 59 age and the decreasing tendency after 59 age. 2) In the normal subject the difference of serum lipid value between sexes did not make the significant difference of level statistically in all items which can be inspected. 3) The weight change and serum lipid value are very significantly related. Total cholesterol, HDL cholesterol and total lipid value are high in female if the weight is light. But they are high in male the weight is heavy phospholipid value is opposite to the above result. Triglyceride value is high in male regardless the weight, but the significant difference happened in the high weight group. 4) As serum lipid value of hypertension patients is compared with that of the normal subjects, HDL cholesterol, triglyceride, phospholipid and total lipid value are significantly high. 5) The doubtful group of the fatty infittration of liver among the hypertension pafients have very significant difference in serum lipid value compared with not only the value of the normal subject but also that of the obesity group. 6) The doubtless group of the fatty infitration of liver among the hypertension patients have the significant difference in all serum lipid value compared with that of the normal subject, but compared with the obesity group males had the significant difference of HDL cholesterol value and both sexes had the significant difference in total lipid value, but had no significant difference in total cholesterol, triglyceride and phosprolipid value. 7) The ratio of total cholesterol per HDL cholesterol had the significant difference in the doubtful hypertension patients of the fatty infittration of liver and the relative weight, but it had no significant difference compared with the normal subject in the doubtless hypertension patients.

• Journal of Microbiology and Biotechnology
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• 제27권12호
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• pp.2173-2179
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• 2017

The intracytoplasmic membrane of Rhodobacter sphaeroides readily vesiculates when cells are lysed. The resulting chromatophore membrane vesicle (CMV) contains the photosynthetic machineries to synthesize ATP by ATPase. The light-dependent ATPase activity of CMV was lowered in the presence of $O_2$, but the activity increased to the level observed under anaerobic condition when the reaction mixture was supplemented with ascorbic acid (${\geq}0.5mM$). Cell lysis in the presence of biotinyl cap phospholipid (bcp) resulted in the incorporation of bcp into the membrane to form biotinylated CMV (bCMV), which binds to streptavidin resin at a ratio of approximately $24{\mu}g$ bacteriochlorophyll a/ml resin. The ATPase activity of CMV was not affected by biotinylation, but approximately 30% of the activity was lost by immobilization to resin. Interestingly, the remaining 70% of ATPase activity stayed constant during 7-day storage at $4^{\circ}C$. On the contrary, the ATPase activity of bCMV without immobilization gradually decreased to approximately 40% of the initial level in the same comparison. Thus, the ATPase activity of CMV is sustainable after immobilization, and the immobilized bCMV can be used repeatedly as an ATP generator.