• 제목/요약/키워드: phase-contrast

검색결과 840건 처리시간 0.023초

Properties of $Zn_xSnO_2$ Nanorods Synthesized by Hytrothermal Method

  • Yeo, Chang-Su;Lee, Gwan-Ho;Kang, Hee-Kyoung;Lee, Kyung-Hee;Yu, Byung-Yong;Song, Jong-Han;Chae, Kuen-Hwa
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2012년도 제42회 동계 정기 학술대회 초록집
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    • pp.313-313
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    • 2012
  • ZnO and $SnO_2$, well-known wide direct band-gap semiconductors, have been considered as the most promising functional materials due to their highly sensitive gas sensing and excellent optical properties. ZnO/$SnO_2$ epitaxial hetrostructure exhibited unique luminescence properties in contrast with individual tetra-pod ZnO and $SnO_2$ nanostructures. Polycrystalline $SnO_2$-based samples $Zn_xSn_{1-x}O_2$(x=0, 0.01, 0.03, 0.05) were prepared by solid state reaction and eco-friendly hydrothermal techniques. Scanning electron microscopy equipped with electron dispersive x-ray spectra confirms the formation of near stoichiometric $Zn_xSn_{1-x}O_2$ nanorods of diameter ~10 nm. X-ray diffraction analysis revealed the rutile structure, except for x=0.07, which may have a small part of $Zn_2SnO_4$ as a secondary phase.

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CuO가 코팅된 Cu 분말을 혼합한 Camphene 슬러리의 동결건조에 의한 Cu 다공체 제조 (Fabrication of Porous Cu by Freeze-drying Process of Camphene Slurry with CuO-coated Cu Powders)

  • 방수룡;오승탁
    • 한국분말재료학회지
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    • 제21권3호
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    • pp.191-195
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    • 2014
  • This study reports a simple way of fabricating the porous Cu with unidirectional pore channels by freeze drying camphene slurry with Cu oxide coated Cu powders. The coated powders were prepared by calcination of ball-milled powder mixture of Cu and Cu-nitrate. Improved dispersion stability of camphene slurry could be achieved using the Cu oxide coated Cu powders instead of pure Cu powders. Pores in the frozen specimen at $-25^{\circ}C$ were generated by sublimation of the camphene during drying in air, and the green bodies were sintered at $750^{\circ}C$ for 1 h in $H_2$ atmosphere. XRD analysis revealed that the coated layer of Cu oxide was completely converted to Cu phase without any reaction phases by hydrogen heat treatment. The porous Cu specimen prepared from pure Cu powders showed partly large pores with unidirectional pore channels, but most of pores were randomly distributed. In contrast, large and aligned parallel pores to the camphene growth direction were clearly observed in the sample using Cu oxide coated Cu powders. Pore formation behavior depending on the initial powders was discussed based on the degree of powder rearrangement and dispersion stability in slurry.

가스침탄 처리한 AISI 8620 강에서 급냉제가 표면잔류응력에 미치는 영향 (Effect of Quenchant Temperature on the Surface Residual Stress in Gas Carburized AISI 8620 Steel)

  • 장충길;한준희;황농문;김종집;임병수
    • 열처리공학회지
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    • 제2권2호
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    • pp.27-32
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    • 1989
  • The effect of quenchant temperature on the surface residual stress was studied for AISI 8620 steel. Specimens were carburized at $900^{\circ}C$ in all case type furnace using a gas-base atmosphere of methanol cracked and liquefied petroleum gas, and then subjected to single reheat quenchant in oil or salt bath in the temperature range of $60^{\circ}C$ to $300^{\circ}C$. After carburizing and reheat Quenching, residual stress was measured by the hole drilling method. Experimental results showed that the surface residual stress was increased as the quenchant temperature was raised. This is in contrast to the fact that the formation of phase of low transformation strain such as bainite results in lower surface compressive stress. The greater compressive stress observed in specimens Quenched at higher temperature may be attributed to the shifting of the transformation start point farther from the surface, as was reported in other carburizing steels.

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Helicobacterpylori에 감염된 위상피세포에서 14-3-3 결합 단백질의 변화 (14-3-3-Associated Proteins in Helicobacter pylori-Infected Gastric Epithelial Cells)

  • 정혜연
    • 한국식품영양학회지
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    • 제24권2호
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    • pp.258-267
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    • 2011
  • 14-3-3 is a highly conserved, ubiquitously expressed protein family. It associates with diverse cellular proteins through its specific phosphoserine/phosphothreonine-binding activity and thus contributes to the regulation of crucial cellular processes such as metabolism, signal transduction, cell-cycle control, apoptosis, protein trafficking, transcription and stress responses. This study aims to determine changes in levels of 14-3-3 isoforms and 14-3-3 - associated proteins in Helicobacter pylori(H. pylori)-infected gastric epithelial AGS cells. AGS cells were stimulated with H. pylori(NCTC 11637) at the ratio of 300:1(bacterium:cell). Western blot analysis revealed that 14-3-3 $\sigma$ was elevated at 3 hr after H. pylori treatment. Other isoforms were not significantly affected by H. pylori infection. Using immunoprecipitation to 14-3-3 $\sigma$, followed by proteomic analysis, we found that S phase kinase associated protein isoform 2 bound to 14-3-3 $\sigma$ has increased. In contrast, three proteins (DEAD-box polypeptide 3, heterogeneous nuclear ribonucleoprotein H2 and WD repeat-containing protein isoform 1) bound to 14-3-3 decreased by H. pylori infection. Our results suggest that 14-3-3 may play an important regulatory role in H. pylori-induced signal transduction in gastric epithelial cells.

THE CYCLIC VARIATION OF SOLAR PHOTOSPHERIC INTENSITY FROM SOHO IMAGES

  • Jeong, Dong-Gwon;Park, Hyungmin;Moon, Byeongha;Oh, Suyeon
    • 천문학회지
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    • 제50권4호
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    • pp.105-109
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    • 2017
  • The well-known solar cycle controls almost the entire appearance of the solar photosphere. We therefore presume that the continuous emission of visible light from the solar surface follows the solar cyclic variation. In this study, we examine the solar cyclic variation of photospheric brightness in the visible range using solar images taken by the Solar and Heliospheric Observatory (SOHO)/Michelson Doppler Imager (MDI). The photospheric brightness in the visible range is quantified via the relative intensity acquired from in the raw solar images. In contrast to total solar irradiance, the relative intensity is out of phase with the solar cycle. During the solar minimum of solar cycles 23-24, the relative intensity shows enhanced heliolatitudinal asymmetry due to a positive asymmetry of the sunspot number. This result can be explained by the strength of the solar magnetic field that controls the strength of convection, implying that the emission in the visible range is controlled by the strength of convection. This agrees with the photospheric brightness increasing during a period of long spotless days.

Isolation and characterization of unrecorded yeasts species in the family Metschnikowiaceae and Bulleribasidiaceae in Korea

  • Park, Yuna;Maeng, Soohyun;Srinivasan, Sathiyaraj
    • Journal of Species Research
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    • 제9권3호
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    • pp.198-203
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    • 2020
  • The goal of this study was to isolate and identify wild yeasts from soil samples. The 15 wild yeast strains were isolated from the soil samples collected in Pocheon city, Gyeonggi Province, Korea. Among them, four yeast stains were unrecorded, and 11 yeast stains were previously recorded in Korea. To identify wild yeasts, microbiological characteristics were observed by API 20C AUX kit. Pairwise sequence comparisons of the D1/D2 domain of the 26S rRNA were performed using Basic Local Alignment Search Tool(BLAST). Cell morphology of yeast strains was examined by phase contrast microscope. All strains were oval-shaped and polar budding and positive for assimilation of glucose, 2-keto-ᴅ-gluconate, N-acetyl-ᴅ-glucosamine, ᴅ-maltose and ᴅ-saccharose (sucrose). There is no official report that describes these four yeast species: one strain of the genus Kodamaea in the family Metschnikowiaceae and three strains of the Hannaella in the family Bulleribasidiaceae. Kodamaea ohmeri YI7, Hannaella kunmingensis YP355, Hannaella luteola YP230 and Hannaella oryzae YP366 were recorded in Korea, for the first time.

미세 원형 충돌수제트의 부분 대류비등에 있어서 자유표면/잠입 제트의 국소 열전달 특성 (Local Heat Transfer Characteristics in Convective Partial Boiling by Impingement of Free-Surface/Submerged Circular Water Jets)

  • 조형희;우성제;신창환
    • 설비공학논문집
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    • 제14권6호
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    • pp.441-449
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    • 2002
  • Single-phase convection and partial nucleate boiling in free-surface and submerged jet impingements of subcooled water ejected through a 2-mm-diameter circular pipe nozzle were investigated by local measurements. Effects of jet velocity and nozzle-to-imping-ing surface distance as well as heat flux on distributions of wall temperature and heat transfer coefficients were considered. Incipience of boiling began from far downstream in contrast with the cases of the planar water jets of high Reynolds numbers. Heat flux increase and velocity decrease reduced the temperature difference between stagnation and far downstream regions with the increasing influence of boiling in partial boiling regime. The chance in nozzle-to-impinging surface distance from H/d=1 to 12 had a significant effect on heat transfer around the stagnation point of the submerged jet, but not for the free-surface jet. The submerged jet provided the lower cooling performance than the free-surface jet due to the entrainment of the pool fluid of which temperature increased.

Development of New Reverse Micellar Microencapsulation Technique to Load Water-Soluble Drug into PLGA Microspheres

  • Kim Hyun Joo;Cho Mi Hyun;Sah Hong Kee
    • Archives of Pharmacal Research
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    • 제28권3호
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    • pp.370-375
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    • 2005
  • The objective of this study was to develop a new reverse micelle-based microencapsulation technique to load tetracycline hydrochloride into PLGA microspheres. To do so, a reverse micellar system was formulated to dissolve tetracycline hydrochloride and water in ethyl formate with the aid of cetyltrimethylammonium bromide. The resultant micellar solution was used to dissolve 0.3 to 0.75 g of PLGA, and microspheres were prepared following a modified solvent quenching technique. As a control experiment, the drug was encapsulated into PLGA microspheres via a conventional methylene chloride-based emulsion procedure. The micro­spheres were then characterized with regard to drug loading efficiency, their size distribution and morphology. The reverse micellar procedure led to the formation of free-flowing, spherical microspheres with the size mode of 88 ~m. When PLGA microspheres were prepared follow­ing the conventional methylene chloride-based procedure, most of tetracycline hydrochloride leached to the aqueous external phase: A maximal loading efficiency observed our experimental conditions was below $5\%$. Their surfaces had numerous pores, while their internal architecture was honey-combed. In sharp contrast, the new reverse micellar encapsulation technique permitted the attainment of a maximal loading efficiency of 63.19 $\pm$$0.64\%$. Also, the microspheres had smooth and pore-free surfaces, and hollow cavities were absent from their internal matrices. The results of this study demonstrated that PLGA microspheres could be successfully prepared following the new reverse micellar encapsulation technique.

Deep neural network based prediction of burst parameters for Zircaloy-4 fuel cladding during loss-of-coolant accident

  • Suman, Siddharth
    • Nuclear Engineering and Technology
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    • 제52권11호
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    • pp.2565-2571
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    • 2020
  • Background: Understanding the behaviour of nuclear fuel claddings by conducting burst test on single cladding tube under simulated loss-of-coolant accident conditions and developing theoretical cum empirical predictive computer codes have been the focus of several investigations. The developed burst criterion (a) assumes symmetrical deformation of cladding tube in contrast to experimental observation (b) interpolates the properties of Zircaloy-4 cladding in mixed α+β phase (c) does not account for azimuthal temperature variations. In order to overcome all these drawbacks of burst criterion, it is reasoned that artificial intelligence technique may be a better option to predict the burst parameters. Methods: Artificial neural network models based on feedforward backpropagation algorithm with logsig transfer function are developed. Results: Neural network architecture of 2-4-4-3, that is model with two hidden layers having four nodes in each layer is found to be the most suitable. The mean, maximum, and minimum prediction errors for this optimised model are 0.82%, 19.62%, and 0.004%, respectively. Conclusion: The burst stress, burst temperature, and burst strain obtained from burst criterion have average deviation of 19%, 12%, and 53% respectively whereas the developed neural network model predicted these parameters with average deviation of 6%, 2%, and 8%, respectively.

Suppression of Arsenic Trioxide-induced Apoptosis in HeLa Cells by N-Acetylcysteine

  • Han, Yong Hwan;Kim, Sung Zoo;Kim, Suhn Hee;Park, Woo Hyun
    • Molecules and Cells
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    • 제26권1호
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    • pp.18-25
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    • 2008
  • Arsenic trioxide (ATO) can affect many biological functions such as apoptosis and differentiation in various cells. We investigated the involvement of ROS and GSH in ATO-induced HeLa cell death using ROS scavengers, especially N-acetylcysteine (NAC). ATO increased intracellular ${O_2}^{{\cdot}-}$ levels and reduced intracellular GSH content. The ROS scavengers, Tempol, Tiron and Trimetazidine, did not significantly reduce levels of ROS or GSH depletion in ATO-treated HeLa cells. Nor did they reduce the apoptosis induced by ATO. In contrast, treatment with NAC reduced ROS levels and GSH depletion in the ATO-treated HeLa cells and prevented ATO-induced apoptosis. Treatment with exogenous SOD and catalase reduced the depletion of GSH content in ATO-treated cells. Catalase strongly protected the cells from ATO-induced apoptosis. In addition, treatment with SOD, catalase and NAC slightly inhibited the G1 phase accumulation induced by ATO. In conclusion, NAC protects HeLa cells from apoptosis induced by ATO by up-regulating intracellular GSH content and partially reducing the production of ${O_2}^{{\cdot}-}$.