• Title/Summary/Keyword: phase center

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Determination of Neurotoxin Gene Expression in Clostridium botulinum Type A by Quantitative RT-PCR

  • Shin, Na-Ri;Shin, Ji-Hun;Chun, Jeong Hoon;Yoon, So-Yeon;Kim, Bong Su;Oh, Hee-Bok;Rhie, Gi-eun
    • Molecules and Cells
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    • v.22 no.3
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    • pp.336-342
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    • 2006
  • Real time reverse transcription (RT)-PCR was used to quantify the expression of the botulinum neurotoxin type A (BoNT/A) gene (cntA) by normalization with the expression of 16S rRNA. The method were confirmed by monitoring the mRNA levels of cntA during growth in five type A strains. In all but one of the strains the expression of cntA mRNA was maximal in the late exponential phase, and approximately 35-fold greater than in the early exponential phase. The concentration of the extracellular BoNT/A complex detected by ELISA was highest in stationary phase. Sodium nitrite and sorbic acid completely inhibited growth at 20 ppm and $4mg\;ml^{-1}$, respectively. CntA expression became lower in proportion to the concentration of sorbic acid, and this reduction was confirmed by mouse bioassay. Our results show that real time RT-PCR can be used to quantify levels of C. botulinum type A neurotoxin transcripts and to assess the effects of food additives on botulinal risk.

A Torque Angle Control of Permanent Magnet Synchronous Motors (영구자석 동기 전동기의 토오크 각 제어)

  • Choi, U.D.;Jung, M.K.;Lee, H.S.;Kim, M.C.
    • Proceedings of the KIEE Conference
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    • 1990.11a
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    • pp.336-339
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    • 1990
  • The permanent magnet synchronous motor windings are energized by sinusoidal excitation current. The frequency of the winding excitation current is synchronous with motor movement and the phase is a function of the motor position with respect to the stator. The total operational speed range of the system is substantially incresed by controlling the phase of the excitation currents at a function of the desired speed. This becomes the torque angle between stator rotating field and motor position. In this paper, torque angle control method is described for surface permanent magnet synchronous motor (SPMSM). The control circuit for realizing control method is investigated and the system test is carried out.

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The Characteristics Analysis of Single Phase LSPM Synchronous Motor by changing Design Parameter (단상 LSPM 동기 전동기의 설계 변수 변화에 따른 특성 해석)

  • Hong, Sook-Hyun;Ko, Kwon-Min;Park, Chan-Bae;Choi, Jae-Young
    • Proceedings of the KIEE Conference
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    • 2003.10b
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    • pp.86-88
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    • 2003
  • The efficiency of electric machine is important due to increase of interest about energy saving. Single Phase Line Start Permanent Magnet Synchronous Motor has high efficiency and power factor. LSPMSM offer a high efficiency as compared induction motor which are used in the home appliance. The analysis and design of LSPMSM is difficult because of unbalanced rotating magnetic field, nonlinear characteristics and rotor saliency. To consider these effects, F.E.M(Finite Element Methods) is coupled equivalent circuit methods. In this paper, a methods of analysis and design using F.E.M and equivalent circuit is represented.

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Starting Characteristic Analysis of 2.8kW Single-phase LSPM Motor (단상 2.8kW급 LSPM 모터의 기동특성 해석)

  • Lee, Jeong-Jong;Rhyu, Se-Hyun;Kim, Young-Kyoun;Jung, In-Soung
    • Proceedings of the KIEE Conference
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    • 2011.07a
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    • pp.1067-1068
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    • 2011
  • This paper deals with the method of starting characteristic analysis of a single-phase line-start permanent magnet (LSPM)motor. The starting characteristic such as current, torque and is important in LSPM because of demagnetizing of the permanent magnet. In this paper, the characteristic analysis is performed using finite element method.

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Real-time Temporal Characterization and Performance Optimization of a kHz Femtosecond Ti:Sapphire Laser Using a Comprehensive SPIDER

  • Luu, Tran Trung;Park, Ju-Yun;Lee, Jae-Hwan;Nam, Chang-Hee
    • Journal of the Optical Society of Korea
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    • v.14 no.2
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    • pp.146-151
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    • 2010
  • A comprehensive real-time spectral phase interferometry for direct electric field reconstruction (SPIDER) apparatus for characterizing femtosecond laser pulses is demonstrated. The SPIDER provides the temporal profiles of femtosecond laser pulses, reconstructed at the speed of 3.5 Hz, with parameters of the spectral phase such as group delay dispersion and third-order dispersion. The apparatus is applied successfully to optimize the spectral dispersion of a kHz femtosecond Ti:Sapphire laser by adjusting a grating compressor in real time.

A Vacuum Circuit Breaker Model for simulation of Transient Overvoltages Using the PSCAD/EMTDC (PSCAD/EMTDC를 이용한 과도전압 모의를 위한 진공차단기 모델 개발)

  • Lee, Wook-Hwa;Lee, J.H.;Lee, Jin;Choi, J.W.
    • Proceedings of the KIEE Conference
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    • 2002.07a
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    • pp.232-234
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    • 2002
  • A Vacuum Circuit Breaker Model for simulation of transient overvoltages was developed by using the PSCAD/EMTDC. The Model includes the power frequency chopping current. Breaker withstand voltage, and quenching capability of High frequency currents. To verify the abilities of the breaker model, the simulation on the Lindmayer's single-phase circuit was carried out. The results show that using the proposed model, the transient overvoltages, current chopping, and multiple reignitions of vacuum breaker are properly reproduced as real phenomena. In further study, virtual chopping current will be incorporated into the model for 3 phase system.

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Low Temperature Synthesis of Willemite Powder (Willemite 분말의 저온합성)

  • Son, Se-Gu;Lee, Ji-Hyeon;Lee, Jeong-Mi;Kim, Young-Do
    • Journal of the Korean Ceramic Society
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    • v.45 no.7
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    • pp.401-404
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    • 2008
  • Willemite ($Zn_2SiO_4$) are a wide range of applications such as a phosphor host and an important crystalline phase in glass ceramics, electrical insulators, glazes, and pigments. In this study, Willemite precursors were synthesized with zinc silicate gels from mixture of zinc nitrate solution and various sodium silicate solution by the geopolymer technique. To examine the crystallization behavior, precursors were have been monitored by the XRD. A pure willemite phase was obtained at $900^{\circ}C$. TEM investigations revealed that the sample with 50 nm particle size was obtained via heat-treated at $900^{\circ}C$ for W-3.

Improvement of Phase Separation Behavior of LC/Polymer Composite by using PDLC Prepolymer

  • Song, Seong-Kyu;Jung, Eun-Ae;Sung, Shi-Joon;Kim, Dae-Hwan;Kang, Jin-Kyu;Ha, Ki-Ryong
    • 한국정보디스플레이학회:학술대회논문집
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    • 2009.10a
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    • pp.931-934
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    • 2009
  • EHA(ethylhexyl acrylate), well known for the prepolymer used for PDLC, was used for the LC/polymer composite system for pixel isolated LC (PILC). In order to improve the polymer wall structures of EHA, various acrylate prepolymers were blended with EHA prepolymer.

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Developing Of Cascaded NPC Multilevel Inverter (Cascaded NPC 고압인버터 개발)

  • Park, Jong-Je;Yun, Hong-Min;Yoo, An-No;Jang, Dong-Je
    • Proceedings of the KIPE Conference
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    • 2013.07a
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    • pp.45-46
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    • 2013
  • 멀티레벨 고압인버터 토폴로지 중 저압 Power Cell를 이용하여 고압을 출력하는 Cascaded 방식이 산업계에서는 널리 사용되고 이다. 최근 제품화된 Cascaded 방식은 크게 두 가지 형태로 구분할 수 있다. 저압 Power Cell에 단상 H-Bridge를 이용한 Cascaded H-Bridge 멀티레벨 인버터와 단상 NPC(Neutral Point Clamped) 토폴로지를 적용한 Cascaded NPC 멀티레벨 인버터이다. 이 중 Cascaded NPC 멀티레벨 인버터의 경우 적은 수의 셀을 사용하여 CHB와 동일한 출력 레벨을 생성할 수 있으며, NPC 방식의 고압 인버터 고유의 장점을 모두 구현할 수 있다. 반대로 CHB Type과 NPC type의 단점인 복잡한 구조의 Phase Shift Transformer와 DC_Link 중성점 전압이 변동하는 단점 또한 나타나게 된다. 본 논문에서는 Cascaded NPC 멀티레벨 인버터의 이러한 단점을 극복하기 위한 새로운 방식의 Phase Shift Transformer와 NPC Power Cell의 중성점 전압 변동을 줄일 수 있는 기법에 대해 설명하고 이 기법에 대한 타당성을 모의시험을 통해 검증하였다.

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