• Journal of the Korean Applied Science and Technology
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• v.3 no.1
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• pp.39-42
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• 1986

The antioxidant activity of petroleum ether extract of Panax ginseng roots in the oxidation of mixed methyl esters of unsaturated fatty acids(MEUFA) was investigated in vitro. The petroleum ether extract of panax ginseng roots showed the antioxidant activity and inhibited the weight gain in the autoxidation of MEUFA. And the induction periods in the autoxidation of MEUFA were related to te addition concentrations of petroleum ether extact. The antioxidant effect of petroleum ether extract on the autoxidation of MEUFA was caused by the protective formation of lipid peroxides and carbonyl compounds. From the results obtained, it was confirmed that petroleum ether extract of panax ginseng roots contained antioxidant substances.

• KSBB Journal
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• v.15 no.2
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• pp.162-166
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• 2000

This study was pe바ormed to observe the effects of Platycodon grandiflorum A. DC (3 years) extracts on the bronchus d diseases bacteria(Mycobacterium sp., K. pneumoniae, F. nucleatum, S. aureus, C. diphtheriae, S. pygogenes and N N. gonorrhoeae) and fungi(A. fumigatus). Platycodon grandiflorum A. DC was extracted by ethanol, water, ethyl ether and p petroleum ether. The extraction rates of Platycodon grandiflorum A. DC were identified as 71.8%, 100%, 15.4% and 14.1% i in each extract solution. Each extract solution was injected into culture media with several concentrations and then the b bacteria cell growth was investigated during 32 hours. As a result, the antimicrobial activities of extracts from ethyl ether and p petroleum ether were excellent. Among several concentrations, the percentage of bacteria cell growth inhibitions were observed to be from 0.06% to 0.14%. The rates of antimicrobial activities were over 70%. The degree of cell growth | inhibition of each bacteria was appeared in the order of ethyl ether > petroleum ether > water > ethanol.

• Environmental Analysis Health and Toxicology
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• v.3 no.3_4
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• pp.1-8
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• 1988

Experiment was performed to investigate the immunotoxicity of cadmium administered orally and the effect of ginseng petroleum ether fraction on it. Mice were given 3, 30, or 300 ppm cadmium as cadmium chloride orally in the drinking water and injection of ginseng petroleum ether fraction intraperitoneally for 4 weeks. Mice were sensitized and challenged u'ith sheep red blood cells (5-HBC). Immune response was evaluated by delayed type hypersensitivity (DTH), Rosette forming cell (RFC), phagocyte activity, and natural killer cell activity (NK cell activity). In the present study, cadmium suppressed the cellular immunity, It also depressed phagocyte activity very significantly in all cadmium-administered groups, NK cell activity in the cadmium-300 ppm administered group. Ginseng petroleum ether fraction showed restoring effect on the decrease in RFC by cadmium-administration. Remarkably, it showed very significant restoring effect on the depression of phagocyte activity induced by cadmium-administration. From this result, we suppose that the anti-tumor effect of ginseng ether or petroleum ether extract, which has been reported by some other researchers, is mainly due to the increase of phagocyte activity by it's administration.

• Journal of Pharmacopuncture
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• v.17 no.3
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• pp.7-15
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• 2014

Objectives: The present study investigated the toxic properties of petroleum ether extract of Wattakaka (W.) volubilis in Wistar female rats. Methods: An in vitro brine shrimp lethality bioassay was studied in A. Salina nauplii, and the lethality concentrations were assessed for petroleum ether extract of W. volubilis. A water soluble portion of the test extract was used in different concentrations from $100-1000{\mu}g/mL$ of 1 mg/mL stock solution. A 24-hours incubation with a 1-mL aliquot in 50 mL of aerated sea water was considered to calculate the percentage rate of dead nauplii with test extract administration against a potassium-dichromate positive control. The acute and the sub-acute toxicities of petroleum ether extract of W. volubilis were evaluated orally by using gavage in female Wistar rats. Food and water intake, body weight, general behavioral changes and mortality of animals were noted. Toxicity or death was evaluated following the administration of petroleum ether extract for 28 consecutive days in the female rats. Serum biochemical parameters, such as alanine aminotransferase (ALT), alkaline phosphatase (ALP), bilirubin, total cholesterol, triglyceride, total protein, glucose, urea, creatinine, sodium, potassium and ${\alpha}$-amylase levels, were measured in the toxicity evaluations. Pathological changes in isolated organs, such as the liver, kidneys, and pancreas, were also examined using hematoxylin and eosin dye fixation after the end of the test extract's administration. Results: The results of the brine-shrimp assay indicate that the evaluated concentrations of petroleum ether extract of W. volubilis were found to be non-toxic. In the acute and the sub-acute toxicity evaluations, no significant differences were observed between the control animals and the animals treated with extract of W. volubilis. No abnormal histological changes were observed in any of the animal groups treated with petroleum ether extract of W. volubilis. Conclusion: These results suggest that petroleum ether extract of W. volubilis has a non-toxic effect in Wistar female rats.

• The Korean Journal of Food And Nutrition
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• v.8 no.2
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• pp.105-109
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• 1995

This study was devised to observe the inhibitory effects of 3 kinds of petroleum ether extracts (percolation by petroleum ether) from Korean red ginseng, Chinese red ginseng and American white ginseng on a lipolytic action of Toxohormone-L which has been known as lipolytic and anorexigenic factors. Toxohormone-L was obtained by partial purification of the ascites fluid from mice which had been Inoculated with sarcoma-180. The yields of petroleum ether extract from Korean red ginseng, Chinese red ginseng and American white ginseng were 0.64, 0.47 and 0.58 and respectively, indicating that the yield of Korean red ginseng was the highest. In vitro, at the concentration of 2 mg /ml, the inhibition rate of lipolysis by the petroleum ether extract of Korean red ginseng, Chinese red ginseng and American white ginseng were 55.1, 50.0 and 44.9% respectively, and the total inhibitory activity per gram of ginseng material were 18, 12 and 13 unit respectively, indicating that the Korean red ginseng was the most effective in the inhibition of the lipolysis.

• Transactions of the Korean Society of Automotive Engineers
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• v.16 no.2
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• pp.158-165
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• 2008

The combustion and exhaust emissions characteristics of a liquefied petroleum gas-di-methyl ether compression ignition engine with a variable valve timing device were investigated under various liquefied petroleum gas injection timing conditions. Liquefied petroleum gas was used as the main fuel and was injected directly into the combustion chamber. Di-methyl ether was used as an ignition promoter and was injected into the intake port. Different liquefied petroleum gas injection timings were tested to verify the effects of the mixture homogeneity on the combustion and exhaust emission characteristics of the liquefied petroleum gas-di-methyl ether compression ignition engine. The average charge temperature was calculated to analyze the emission formation. The ringing intensity was used for analysis of knock characteristics. The combustion and exhaust emission characteristics differed significantly depending on the liquefied petroleum gas injection and intake valve open timings. The CO emission increased as the intake valve open and liquefied petroleum gas injection timings were retarded. However, the particulate matter emission decreased and the nitrogen oxide emission increased as the intake valve open timing was retarded in the diffusion combustion regime. Finally, the combustion efficiency decreased as the intake valve open and liquefied petroleum gas injection timings were retarded.

• Environmental Analysis Health and Toxicology
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• v.3 no.1_2
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• pp.43-54
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• 1988

Experiment was performed to investigate the immunotoxicity of cadmium administered orally and the effect of ginseng petroleum ether fraction on it. Mice were given 3, 30, or 300 ppm cadmium as cadmium chloride orally in the drinking water and injection of ginseng petroleum ether fraction intraperitoneally for 4 weeks. Mice were sensitized and challenged with sheep red blood cells (S-RBC). Humoral immune response was evaluated by antibody production and Arthus reaction. Body weight gain, spleen weight, thymus weight, and liver weight were also measured. In the present study, cadmium generally suppressed the humoral immunity. There was decrease in the rate of body weight gain and liver weight to body weight by cadmium-administration. Ginseng petroleum ether fraction showed restoring effect, to some extent, on the decrease in PFC and in the rate of liver weight to body weight by cadmium-administration. But it more suppressed Arthus reaction.

• Journal of Ginseng Research
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• v.20 no.3
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• pp.219-225
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• 1996

This study was performed to investigate the inhibition mechanism of cancer cell proof iferation caused by the petroleum-ether extract of ginseng against human rectum (HRT-18), colon (HT-29), llepatoma (Hep G2) and prostate (LNCaP) cancer cells and monkey kidney cells (Vero 76). Cells were treated with the petroleum-ether extract of ginseng (50 to 200 $\mu\textrm{g}$/ml) in G1 or S phase of the cell cycle, and proliferation and protein kinase C activity were measured. The petroleum-eth or extract of ginseng inhibited proliferation of HRT-18, HT-29, Hep G2 and LNCaP when treated in Gl phase, but not in S phase. This result shows that the ginseng extract arrests the cell cycle in G1 phase, resulting in the inhibition of cell proliferation. At the same concentrations, treatment of the ginseng extract in G1 phase decreased protein kinase C activity, while the treatment in S phase had no effect. This reault suggests that protein kinase C might be involved in the inhibition of the cell cycle and proliferation of cancer cells caused by the petroleum-ether extract of ginseng.

• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.305-308
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• 2000

This study was observed to the effect of the feeding Platycodon grandiflorum A. DC (3 years) extract on the bronchus diseases bacteria ( C. diphtheriae, S. aureus, Mycobacterium sp., F. nucleatum, S. pygogenes, K. pneumoniae and N. gonorrhoeae) and fungi(A. fumicatus). Platycodon grandiflorum A. DC was extracted ethanon, water, ethyl ether and petroleum ether. The extraction rate of Platycodon grandiflorum A. DC to the extract solution was identified 71.8%, 100%, 15.4% and 14.1%. Each extract solution was injected culture media into several concentrations and then investigated the bacteria cell growth during 32 hours. As a result antimicrobial activity was excellent an extract by ethyl ether and petroleum ether. Among several concentrations, bacteria cell growth inhibition was observed from 0.06% to 0.14%. The rate of antimicrobial activity was over 70%. The cell growth inhibition rate of each bacteria was appeared in order of ethyl ether > petroleum ether > water > ethanol.

• Journal of Ginseng Research
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• v.10 no.2
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• pp.141-150
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• 1986

This study was attempted to screen the cytotoxic activity of petroleum ether ex- tract from panax ginseng root against human colon cancer cells. Two extracts of panax ginseng root, crude and partially purified, were used for this experiment. The crude extract was prepared by extraction with petroleum ether using Soxhlet aparatus for 12 to 15 hours from panax ginseng and the extract was partially purified by silicic acid column with mixture of petroleum ether: ethyl ether (70 : 30, v/v). Three species of human colon cancer cells, HRT-18, HCT-48 and HT-29, were maintained in DMEM (Dulbecco's modified Eagle medium), and the cells were cultured in DMEM containing serial concentration of the crude or partially purified fraction to observe the cytotoxic activity of the both extracts. The effects of incubation time and concentration of the both extracts in culture medium against the growth of the each cancer cell were determined. The results obtained are summarized as follows: 1. The doubling times of the HRT-18, HCT-48 and HT-29 cells were about 20, 24 and 22 hours, respectively. 2, The inhibitory effects of the crude extract on the growth of cancer cells were increased according to the rise of concentration of the extract and incubation time. 3. The inhibitory effect of partially purified fraction on the growth of HRT-18 cell was about 4 times stronger than that of the crude extract under same experimental condition. 4 The inhibitory effects of the crude and purified fraction on the growth of each cancer cell were shown difference by the kind of the cancer cell. In view of the above results, it could be said that the petroleum ether extract of panax ginseng root inhibited the division of the human colon cancer cell, in vitro.