• 한국유기농업학회지
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• 제19권4호
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• pp.529-541
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• 2011

Bordeaux mixture made with each grade of lime and copper sulfate was tested general property and disease control value. Bordeaux mixture was made by composition of each grade of lime (95%, 80%) with each grade of copper sulfate(98.5%, 95%). Phytotoxicity was evaluated to Bordeaux mixture made with each grade of lime and copper sulfate, and then low grade copper sulfate (95%) was more severely in the grapevine leaf. Bordeaux mixture, made with each grade lime and copper sulfate, were tried to evaluate control effects of downy mildew in field. As a result, Bordeaux mixture (95% of lime and 98.5% of copper sulfate, 6-6) applied 5 times at late in June was showed more effective disease control value than any other trials of Bordeaux mixture. There was no difference in nutrient status of petiole each treatment. But fruit characteristics were shown more slightly improved quality.

• 식물조직배양학회지
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• 제27권2호
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• pp.137-142
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• 2000

천궁 (Cnidium officinale)의 잎, 엽병 및 화기 외식편을 재료로 하여 2,4-D가 첨가된 배지에 배양하였을때 캘러스 형성은 어느 외식편에서나 비슷한 수준으로 유기되었지만 배형성능 캘러스는 화기유래 캘러스에서만 형성되었다. 체세포배발생의 유도는 2mg/L 2,4-D를 첨가한 MS배지에서 잘 일어났으며 2,4-D제거배지에서 계대배양하면 외식편당 78개의 체세포배가 형성되었다. 체세포배의 자엽수에 있어서 변이가 빈번하게 관찰되었으나 배지에 1% 활성탄을 첨가하면 비정상적인 자엽수를 갖는 체세포배의 발생률이 감소되는 효과를 얻을 수 있었다. 자엽기 체세포배는 MS 기본배지에서 저조한 발아율을 보이는 반면에 활성탄 첨가배지에서는 현저한 발아 향상을 보였다.

• Journal of Plant Biotechnology
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• 제43권2호
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• pp.255-260
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• 2016

This research was conducted to improve the cold tolerance of the gerbera cv. Gold Eye by introduction of the Arabidopsis $Ca^{2+}/H^+$ antiporter gene (CAX1) via Agrobacterium-mediated transformation. Prior to genetic transformation, we optimized a combination of plant growth regulators; $1.0mgl^{-1}$ 6-Benzyladenine (BA) and $0.1mgl^{-1}$3-indole-acetic acid (IAA) were found to lead to proper in vitro shoot regeneration from petiole explants. In addition, $50mgl^{-1}$ kanamycin was determined to be the minimal concentration useful for selection of putative transgenic plants. In this study, transgenic gerbera expressing the Arabidopsis $Ca^{2+}/H^+$ antiporter gene (CAX1) were obtained using the optimized concentrations. We expect that introduction of the gene to the cultivar will improve cold tolerance, which will be important in the winter months.

• 한국조경학회지
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• 제27권4호
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• pp.50-58
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• 1999

This study was carried out to investigate the growth characteristics and propagation methods of L. obtusiloba as a woody landscape plant. The results are obtained as follows: L. obtusiloba was dioecious shrub and shaped with bush type. Leaves were ovate, obtuse, cordate, 7.05 cm long, 7.20 cm wide, and petiole 2.0 cm long. Flowers of L. obtusiloba were diclinous. Soil acidity ranged from pH 4.06 to 5.53 with the lowest at the Mt. Soo-Ri. Mt. Soo-Ri located near factory district, which was considered to damaged by environmental deteoration. While soil organic matter was highest at Mt. Soo-Ri, inorganic nutrients were low. L. obtusiloba grows in the area with low soil acidity and low content of inorganic. Therefore it seemed to be tolerant to air pollution. L. Obtusiloba was high seed germination rate in the plug box and its shoots were longer than seeding box and softwood cutting of L. obtusiloba showed the rooting rate of 50% at 5,000ppm on June 23. To develop a mass propagation method of Korean native L. obtusiloba through an axillary bud culture as a woody landscape plant, about 2∼3 cm shoots induced from explant were subcultured to new media contained different growth regulators. Shoots multiplied most effectively on a WPM containing 1.0 mg/l BA, producing 5.5 shoots with a shoot length of 2.5 cm per shoot explant.

• 한국작물학회지
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• 제42권3호
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• pp.281-285
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• 1997

춘천에서 생산된 고추냉이 품종, 대왕001와 달마종 그리고 평창에서 생산된 대왕001의 부위별 신미성분 allylisothiocyanate 함량을 조사한 결과는 다음과 같다. 1. 고추냉이의 식물체 정유함량은 0.1∼0.5％로 뿌리가 가장 높고 엽병이 가장 낮았다. 2. Allylisothiocyanate는 식물체 전부위에서 검출되었는데 근경 부위가 가장 높았고 근＞엽＞엽병 순으로 낮았다. 3. 재배장소 및 품종에 따라 allylisothiocyanate 함량은 차이를 보였는데 춘천 지방이 평창지방보다 그리고 달마종이 대왕001보다 근경부위의 allylisothiocyanate 함량이 유의성 있게 높았다. 4. 근경의 부위 및 크기에 따른 allylisothiocyanate 함량은 근경의 하부에서 가장 높고 상부에서 가장 낮았으며 근경이 크고 무거울수록 그함량이 높았으나 유의성은 없었다

• 한국작물학회지
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• 제32권3호
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• pp.353-356
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• 1987

하우스 딸기 재배에 있어서 2, 4-D(아민감 40%)의 업면시용 효과를 알기 위하여 1 화방의 1번과 개화기(2월 11 일)에 10ppm 농도로 10 a당 120$\ell$를 처리하였던 결과, 1. 딸기는 엽병장 및 과경장이 길어지면서 상편생장을 하였고 잎과 과실로부터 에틸렌 생성이 증가되었다. 2 딸기는 에틸렌 생성 증가에 따른 수확최감기가 15일 정도 촉진되었고 과실비대로 1 과중이 증가되어 18 %의 증수 효과를 가져왔다.

• Tribology and Lubricants
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• 제33권6호
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• pp.251-255
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• 2017

In this study, we aimed to clarify the wettability and anisotropic flow characteristics of rice leaves as a basic study for engineering applications of anisotropic flow characteristics of rice leaf surface. To investigate the surface structure of rice leaf, the micro grooves and asperities of rice leaves were analyzed and quantified by scanning electron microscope, Confocal laser scanning microscopy, and stylus profilometer. The analysis of the structure of rice leaf surface confirmed that asymmetrical cone - like protrusions in leaf veins were inclined toward the leaf tip. The static contact angle test showed that the contact angle at the midline vein or leaf vein location where the micropapilla is concentrated is about $20^{\circ}$ higher than the leaf blade position. The contact angles of fresh and dried rice leave were also compared. The dried rice leaves showed a contact angle of about $5^{\circ}$ to $15^{\circ}$ higher than that of fresh leaves, suggesting that the volume of the protrusions decreased as the water was removed, thus reducing the contact area with the droplet. In the contact angle history test the hysteresis in the leaf tip direction was found to be much lower than that in the leaf petiole direction. This results can be explained that asymmetrical cone - like protrusions had a significant effect on the droplet flow characteristics through contact angle hysteresis experiment.

• Natural Product Sciences
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• 제10권4호
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• pp.168-172
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• 2004

In India, Gymnema sylvestre due to the unique property of the plant to antagonize the sweet taste is known as 'Gur-mar'. It has several ethnomedicinal values as various tribals/traditional communities and rural peoples of India find diverse medicinal uses viz. antidiabetic, stomachic, diuretic, and is useful in cough and throat troubles. Besides, it has strong effect on reducing blood sugar. The present communication deals with the detailed pharmacognostical evaluation of the aerial parts of G. sylvestre collected from three places of the country-Varanasi (U.P), Panchmarhi (M.P), Salem (Tami Nadu) and commercial sample procured from local market. The botanical and physico-chemical parameters of all the samples were quite similar though little variations were observed in foaming index, alcohol and water soluble extractives of local sample. The microscopic characteristics of the drug are horse shoe shaped petiole with 3 amphicribal vascular bundles, sieve tubes well developed; anomocytic stomata only on the abaxial surface of the leaf, the fan shaped amphicribal vascular bundle, presence of intraxylary phloem. The TLC fingerprint profile of all the samples was more or less similar only the quantity of some of the compounds varied.

• 식물병연구
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• 제17권1호
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• pp.75-77
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• 2011

전북 전주시 화원에서 Botrytis cinerea에 의한 바이올렛 잿빛곰팡이병이 발생하였다. 병징은 꽃, 잎과 잎자루가 수침상으로 물러지고 부패되었다. 다습조건에서 병반위에 잿빛색의 분생포자가 많이 형성하였다. 균총은 PDA 배지에서 회갈색이며 균핵을 형성하였다. 분생포자는 단포자, 난형 또는 타원형으로 무색 또는 옅은 갈색이고, 크기는 $7{\sim}14{\times}5{\sim}9\;{\mu}m$이었다. 바이올렛에 발생한 병징과 병원균의 균학적 특징을 조사한 결과 B. cinerea Persoon:Fries에 의한 바이올렛 잿빛곰팡이병으로 명명할 것을 제안한다.

• 생명과학회지
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• 제15권2호
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• pp.176-181
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• 2005

Calmodulin 유전자의 발현 조절을 연구하기 위해, 벼 calmodulin promoter (RCaM-2)를 분리하여 GUS (report 유전자)에 융합하였다. GUS 활성은 정단조직, 근단 및 관다발 영역과 같은 성장조직에서 높게 발현되었다. 줄기와 페티올의 transverse 절단부위 GUS 활성은 관다발계의 안쪽에 제한되었으며 관다발계의 외부에 위치한 피층과 표피에서는 강하게 발현된 식물에서도 GUS 활성이 나타나지 않았다. GUS 활성은 어린 조직에서 특이적으로 발현되었으며 상처에 의해서 신속하게 증가하였다. RCaM-2 promoter는 세포분열이 왕성한 어린조직이나 생장점에서 강하게 발현되며 mechanical 신호에 의해서 현저히 유도되었다. 이러한 결과는 RCaM-2 유전자의 5'-flanking 영역이 상처에 의해서 유도되는 발현을 조절하는 것으로 추정된다.