Browse > Article
http://dx.doi.org/10.5010/JPB.2016.43.2.255

In vitro shoot regeneration and genetic transformation of the gerbera (Gerbera hybrida Hort.) cultivar 'Gold Eye'  

Chung, Mi-Young (Department of Agricultural Education, Sunchon National University)
Kim, Min Bae (Department of Agricultural Education, Sunchon National University)
Chung, Yong Mo (Flower Research Institute, Gyeongsangnam-do Agricultural Research & Extension Services)
Nou, Ill-Sup (Department of Horticulture, Sunchon National University)
Kim, Chang Kil (Department of Horticultural Science, Kyungpook National University)
Publication Information
Journal of Plant Biotechnology / v.43, no.2, 2016 , pp. 255-260 More about this Journal
Abstract
This research was conducted to improve the cold tolerance of the gerbera cv. Gold Eye by introduction of the Arabidopsis $Ca^{2+}/H^+$ antiporter gene (CAX1) via Agrobacterium-mediated transformation. Prior to genetic transformation, we optimized a combination of plant growth regulators; $1.0mgl^{-1}$ 6-Benzyladenine (BA) and $0.1mgl^{-1}$3-indole-acetic acid (IAA) were found to lead to proper in vitro shoot regeneration from petiole explants. In addition, $50mgl^{-1}$ kanamycin was determined to be the minimal concentration useful for selection of putative transgenic plants. In this study, transgenic gerbera expressing the Arabidopsis $Ca^{2+}/H^+$ antiporter gene (CAX1) were obtained using the optimized concentrations. We expect that introduction of the gene to the cultivar will improve cold tolerance, which will be important in the winter months.
Keywords
Plant growth regulator; Selective agent; Cold tolerance; Transgenic plants;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Barbosa MHP, Pinto JFBP, Pinto CABP, Innecco R (1994) In vitro propagation of Gerbera jamesonii Bolus ex Hook cv. Appel Bloesem using young capitulum. Revista Ceres, 41:386-395
2 Catala R, Santos E, Alonso JM, Ecker JR, Martinez-Zapater JM, Salinas J (2003) Mutations in the Ca2+/H+ transporter CAX1 increase CBF/DREB1 expression and the cold-acclimation response in Arabidopsis. Plant Cell 15:2940-2951   DOI
3 Chakrabarty D, Datta SK (2008) Micropropagation of gerbera: lipid peroxidation and antioxidant enzyme activities during acclimatization process. Acta Physiologiae Plantarum
4 Chung YM, Cho YC, Kwon OC (2007) A New Yellow Gerbera Cut Flower Cultivar, "Gold Eye" with Strong Peduncle and High Flower Yielding. Korean J. Breed. Sci. 39:86-87
5 Elooma P, Honkaanen J, Puska R, Seppanen P, Helariutta Y, Mehto M, Nevalainen L, Teeri TH (1993) Agrobacterium-mediated transfer of antisense chalcone synthase cDNA to Gerbera hybrida inhibits flower pigmentation. Bio/Technology, 11:505-511
6 Gosal SS; Wani SH; Kang MS (2009) Biotechnology and drought tolerance. J. Crop Improv., 23, 19-54   DOI
7 Hasbullah NA, Taha RM, Awal A (2007) Growth optimization and Rganogenesis of Gerbera jamesonii Bolus ex. Hook f. in vitro. Pakistan Journal of Biological Science, 11;1449-1454
8 Hsieh TH; Lee JT; Yang PT; Chiu LH; Charng Y; Wang YC; Chan MT (2002) Heterology expression of the Arabidopsis Crepeat/dehydration response element binding factor 1 gene confers elevated tolerance to chilling and oxidative stresses in transgenic tomato. Plant Physiol., 129:1086-1094   DOI
9 Knight H; Veale EL; Warren GJ; Knight MR (1999) The sfr6 mutation in Arabidopsis suppresses low temperature induction of genes dependent on the CRT/DRE sequence motif. Plant Cell, 11:875-886
10 Nagaraju V, Srinivas GSL, Lakshmi Sita G (1998) Agrobacteriummediated genetic transformation in Gerbera hybrida. Current Science, 71:630-634
11 Naing AH, Ai TN, Jeon SM, Park KI, Lim SH, Lim KB, Kim CK (2016) Novel antibiotics enhance regeneration and genetic transformation with RsMYB1 gene of recalcitrant chrysanthemum cv. Shinma. Plant Biosystems. doi:10.1080/11263504.2015.1103800   DOI
12 Nowak E, Makowska Z, Kucharska D, Orlikowska T (1997) The influence of initial explant on transformation effectiveness of Gerbera hybrida. Biotechnologia (Poznan), 4:27-38
13 Orlikowska T., Nowak E., Marasek A, Kucharska D (1999) Effects of growth regulators and incubation period on in vitro regeneration of adventitious shoots from gerbera petioles. Plant Cell Tiss. and Organ Cult, 59:95-102   DOI
14 Aswath C, Choudhary ML (2002) Rapid plant regeneration from Gerbera jamesonii Bolus callus cultures. Acta Botanica Croatica, 61:125-134
15 Reynorid JP, Chriquid D, Noin M, Brown S, Marie D (1993) Plant propagation from in vitro leaf culture of several gerbera species. Plant Cell Tiss. and Organ Cult, 33:203-210   DOI
16 Wani SH; Sandhu JS; Gosal SS (2008) Genetic engineering of crop plants for abiotic stress tolerance. In: Advanced Topics in Plant Biotechnology and Plant Biology. Malik CP; Kaur B; Wadhwani C; Eds. MD Publications New Delhi, pp. 149-183
17 Tamminen I; Makela P; Heino P; Palva ET (2001) Ectopic expression of ABI3 gene enhances freezing tolerance in response to abscisic acid and low temperature in Arabidopsis thaliana, Plant J., 25, 1-8   DOI
18 Tyagi P, Kothari SL (2004) Rapid in vitro regeneration of Gerbera jamesonii (H. Bolus ex Hook f.) from different explants. Indian Journal of Biotechnology, 3:584-586
19 Wani SH; Gosal SS (2011) Introduction of OsglyII gene into Indica rice through particle bombardment for increased salinity tolerance. Biol. Plant. 55:536-540   DOI
20 Xi M and Shi JS (2003) Tissue culture and rapid propagation of Gerbera jamesonii. Journal Wanjing Forestry University, 27:33-36