• Korean Journal of Plant Resources
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• v.22 no.5
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• pp.394-402
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• 2009

Adventitious shoots were induced from pinnae, petiole and rhizome in Pteris cretica 'Wilsonii' in order to develop the efficient mass propagation method, using in vitro culture. Only homogenized rhizome segments could regenerate young sporophytes. Efficient regeneration of multiple shoots was obtained on the one-eighth strength MS medium containing 1% sucrose, and $50mg{\cdot}L^{-1}$ $NaH_2PO_4$. To achieve higher rate of regeneration from rhizome segments, rhizome segments were exposed to growth regulators for 2 months and then subcultured on hormone-free medium. The greatest shoot regeneration was obtained by $1{\mu}M$ kinetin with $5{\mu}M$ NAA. BA was effective in formation of GGB (kind of meristems), but they showed low shoot regeneration rate. Plants obtained from present experiments were transplanted to examine good environmental conditions for acclimation. Juvenile plants obtained by the one-eighth strength MS medium showed highest survival rate and vigorous growth at the seedling stage.

• Korean Journal of Medicinal Crop Science
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• v.7 no.1
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• pp.1-6
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• 1999

Basic informations for direct somatic embryo formation in Rehmannja glutinosa Lib. were obtained in 500ml erlenmyer flask. The ratio of ammonium to nitrate nitrogen of 825(mg/l) : 1900(mg/l) was proper condition for somatic embryo formation from stem and petiole explants and 3% sucrose was the most effective carbon source. Full strength MS medium with 2mg/l BA was better than LS medium for somatic embryogenesis. The initial pH 5.7 of medium(full strength MS with 2.0mg/l BA and 0.1mg/l NAA) was good for embryo production. Potassium ion was taken up rapidly within 2 weeks. while $Ca^{++}$ and $Mg^{++}$ ion contents were almost constant during culture period. Sucrose hydrolysis occurred throughout the culture, while glucose and fructose were absorbed simultaneously from the third week of culture.

• Journal of Ginseng Research
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• v.26 no.4
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• pp.213-218
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• 2002

Crystalline calcium oxalate occur throughout near)y all plants species in five major forms; styloids, druses, raphids, prisms and sands. These crystals are known to be distributed in specific tissue such as cortex, xylem, phloem, cambium and epidermis. This research was undertaken to identify the occurrence, type, location and ultrastructure of druse crystals in Panax ginseng. In situ visualization, conventional light microscopy, histochemistry and scanning electron microscopy were applied for these purposes. Druse crystals in ginseng were identified as calcium oxalate by silver nitraterubeanic acid histochemistry. Calcium oxalate crystals are observed in nearly all plant organs such as leaf, petiole, peduncle, stem, rhizome, tap root and lateral root except fine root. Most frequent observation of crystals in the leaf and rhizomes were noticed. Three different types of calcium of oxalate druse crystals were identified by scanning electron microscopy.

• Journal of Ginseng Research
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• v.36 no.2
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• pp.211-217
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• 2012

Endophytic fungi were isolated from various tissues (root, stem, petiole, leaf, and flower stalk) of 3- and 4-year-old ginseng plants (Panax ginseng Meyer) cultivated in Korea. The isolated endophytic fungi were identified based on the sequence analysis of the internal transcribed spacer (ITS), 1-5.8-ITS 2. A morphological characterization was also conducted using microscopic observations. According to the identification, 127 fungal isolates were assigned to 27 taxa. The genera of Phoma, Alternaria and Colletotrichum were the most frequent isolates, followed by Fusarium, Entrophospora and Xylaria. Although 19 of the 27 taxa were identified at the species level, the remainder were classified at the genus level (6 isolates), phylum level (Ascomycota, 1 isolate), and unknown fungal species (1 isolate). Endophytic fungi of 13 and 19 species were isolated from 3- and 4-year-old ginseng plants, respectively, and Phoma radicina and Fusarium solani were the most frequently isolated species colonizing the tissues of the 3- and 4-year-old ginseng plants, respectively. The colonization frequency (CF%) was dependant on the age and tissue examined: the CFs of the roots and stems in the 3-year-old ginseng were higher than the CF of tissues in the 4-year-old plants. In contrast, higher CFs were observed in the leaves and petioles of 4-year-old plants, and endophytic fungi in the flower stalks were only detected in the 4-year-old plants. In conclusion, we detected diverse endophytic fungi in ginseng plants, which were distributed differently depending on the age and tissue examined.

• Korean journal of applied entomology
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• v.42 no.2
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• pp.119-124
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• 2003

The adult of the Cotton caterpillar, Palpita indica (Saunder) laid eggs more on mature leaves than on aged and developing leaves. They laid more on leaves than on petiole and stem, and more on the adaxial than on the abaxial surface side of the leaves. Larvae of the Cotton caterpillar showed their preference in the order of the cucumber (Cucumis sativus), gourd (Lagernaria siceraria), watermelon (Citrullus lanatus) ＞ oriental melon (Cucumis melon L. var makuwa), wax gourd (Benincasa hispida), melon (Cucumis melo), star cucumber (Sicyos angulatus) ＞ sponge cucumber (Lufa cylindrica), cotton (Gossypium indicum). There were no distinct differences among varieties and lines that were collected and hybridized at the Gochang Watermelon Experiment Station in Korea. There was a positive relationship between the leaf area and the degree of damage by the Cotton caterpillar. The feeding amounts of the Cotton caterpillar excrement were gradually increased to 16-18 days after hatching, after that the amounts of excrement were rapidly decreased.

• Korean Journal of Plant Taxonomy
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• v.36 no.4
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• pp.309-333
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• 2006

Examined were the vegetative and reproductive characters such as external morphology, anatomy (leaf petiole, blade and stem), pollen and nutlet for the taxonomic revision of Elsholtzia in Korea. The inflorescence of all studied taxa of Elsholtzia in Korea is secund and 0.5-10 cm long. Leaves are ovate or nearly and $0.2-10{\times}0.2-6.0cm$. The shapes of inflorescence and leaves are very useful taxonomic characters. The stomata types are anomocytic and diacytic. The morphology of nutlet is ovoid and pitted in surface pattern of exocarp. All studied taxa are myxocarpy. The pollen grains are of medium size and subprolate in shape, and have typical bi-reticulate exine surface. Four species are recognized for Korean Elsholtzia by the identification key and descriptions in this study.

• Korean Journal of Plant Taxonomy
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• v.35 no.2
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• pp.115-127
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• 2005

Two anatomical features of leaves, transverse section of midribs and petioles, were examined for nine species of five genera of Thymelaeaceae, Daphne L. -4 spp., Diarthron Turcz. -1 sp., Edgeworthia Meisn. -1 sp., Stellera L. -1 sp., Wikstroemia Endl. -2 sp., using light microscopy and scanning electron microscopy. In terms of shape of upper side, three types were observed, i.e., flattened, depressed, and raised. Outline of petioles is mostly semi-circular or circular in transverse section, with two distinct wings/weakly developed wings or without wings. Other characters besides anatomical features of leaves such as vascular bundle tissues, crystal, tannin are also described in details. Finally, we briefly discussed the systematic significance of the anatomical features of leaves for identification of Korean Thymelaeaceae.

• Journal of Plant Biology
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• v.1 no.2
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• pp.5-6
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• 1958

Scdvm rotundifolium LEE, D. B. sp. nov. Korean name: Doonggunip-qwongui-birum The external characteristics of this species are similar to those of Sedum spectabile BOREAU, but the leaf is rounded, the inflorescence is globose, and the color of the ilower is a deeper purplish-red. Description of this species. Root: Perennial, facisculate, rather thick, with several stems. Stem: Fasciculate,15~25cm. high, a little nutate, reddish, putting forth buds on the lower part in the autumn. Leaf: Fleshy, opposite, discoid or broad ovate, no petiole, red, irregular serrate margin, lcaf"base light cordate or round, width and length both 2. 5~4.5 cm. Flower: Tiny flower making the glomerule on stem apex. Calyx: symscpal, 5 parted, green, lanceolatc_ Corolla: 5 petals, beat-shaped, purplish-red. Stamens: 10, five of them opposile and reat of five alternate with petals, filaments same length as petals, anther red, pollen yellow. Pistils: 5, separate, each ovary triangular rhombus, opposite the petals, stigmas' length about Imm. apexes pointed. Collected on October 22, 1957, and July 26, 1958. Habitat: Growing in the rock of Mt. Choowang, North Kyongsang Province. Korea. Korea.

• Journal of Sericultural and Entomological Science
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• v.46 no.1
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• pp.6-11
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• 2004

We evaluated fruity characteristics to select the desirable mulberry varieties for the production of fruit from 2002 to 2003. Tested items were total yield, weight of single fruit, soluble solids and fruit size including length, width, and petiole length. We started yield of mulberry fruits from 27th of May, and continued for two weeks or three weeks according to mulberry varieties. 'Susungppong', 'Cheongnosang', and 'Jeolgokchosaeng(Chungbuk)' were showed high and similar quantity for two years. In weight of single fruit, 'Jeolgokchosaeng(Chungbuk)' and 'Ficus' were 4.6 g respectively. 14 mulberry varieties were showed more than 17 Brix % in soluble solids. From that results, eight suitable varieties such as 'Jeolgokchosaeng(Chungbuk)', 'Dangsang 7', 'Jangsosang', 'Susungppong', 'Suwonnosang', 'Palcheongsipyung', 'Kangsun', and 'Jukcheonchosaeng' were selected for the production of fruit.

• Korean Journal of Agricultural Science
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• v.2 no.1
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• pp.143-149
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• 1975

To obtain some basic informations on the breeding of Kudzu, 42 individuals were selected and their morphological characteristics were investigated and analyzed. The shape of leaves was classified as 4 types and area of leaves into 5 steps. Internode length was distributed between 13 cm and 37 cm. Significant correlations were recognized between petiole and leaf area, between internode length and internode diameter and between internods diameter and leaf area.