• Title/Summary/Keyword: petiole

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Overexpression of N -terminal lacking mutant HFR1 confers light-independence in a subset of photo-responses

  • Yang Ki-Young;Kim Young-Mi;Song Pill-Soon;Soh Moon-Soo
    • Proceedings of the Korean Society of Potoscience Conference
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    • spring
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    • pp.97-103
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    • 2003
  • Phytochrome controls diverse aspects of plant development in response to the ambient light conditions. HFRl, a basic helix-loop-helix protein, is required for a subset of phytochrome A (phy A)-mediated photo-responses in Arabidopsis. Here, we show that overexpression of HFR1-N105, but not the one of the full-length HFR1, confers exaggerated photo-responses. The transgenic plants overexpressing HFR1- N105 exhibited light-independence in a subset of photo-responses, including germination, de-etiolation, gravitropic hypocotyl growth, and blocking of greening. Overexpression of HFR1-N105 also caused constitutive light-responses in the expression of some light-regulated genes. In addition, the HFR1-N105 overexpressor showed hypersensitive responses under R and FR light, dependently on phyB and phyA, respectively. End-of-day far-red light response and petiole elongation were suppressed in the HFR1-N105 overexpressor plants. Together these results imply that overexpression of HFR1-N105 activated a branch of light signaling, supporting the hypothesis that transcriptional regulation in the nucleus would be the primary mechanism of light signaling in Arabidopsis. We discuss the biotechnological potential of the mutant bHLH protein, HFR1-N105 in regard to suppressed shade avoidance syndrome.

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Seasonal Occurrence and Infection Site of Strawberry Anthracnose (딸기탄저병의 시기별 발생과 감염경로)

  • 김승한;최성용;임양숙;윤재탁;최부술
    • Research in Plant Disease
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    • v.8 no.1
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    • pp.45-49
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    • 2002
  • Seasonal occurrence of strawberry anthracnose in greenhouses caused by Colletotrichum sp. was examined from 1997 through 1999 at three locations, Kyeongju, Goryeong, and Cheongdo in Kyungbuk province, Korea. Also some factors related to the anthracnose infection such as initial infection sites, inoculation methods, and soil nature were studied through in vitro and field experiments. The anthracnose disease begun to occur from 15 days after transplanting in early October, and continued but gradually decreased thereafter for 2 months until December, After transplanting, initial infection mainly occurred through the runner of which the tissue was more susceptible to the anthracnose than those of the leaf and petiole when the fungal mycelial disk was inoculated. Postplanting inoculation by irrigation with spore suspension was much more effective in inducing the anthracnose disease than preplanting soil mix. However without inoculation, no or little anthracnose occurred regardless of commercial, non-cultivated or diseased field soils when healthy seedlings were planted. This suggests that occurrence of strawberry anthracnose in fields may be related to contamination of plant propagules with the anthracnose pathogen.

Cultural and Pathogenic Characteristics between Colletotrichum gloeosporioides and Glomerella cingulata Isolated from Strawberry in Korea (국내 딸기 탄저병균 Colletotrichum gloeosporioides와 Glomerella cingulata의 배양적, 병원학적 특성)

  • 남명현;정석기;유성준;서관석;김홍기
    • Korean Journal Plant Pathology
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    • v.14 no.6
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    • pp.654-660
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    • 1998
  • Colletotrichum gloeosporioides and Glomerella cingulata are the most important pathogens causing anthracnose which may reduce the stand rate and yield on wide kinds of plants including strawberry. Average occurrence rate of anthracnose is 36.9% on major strawberry cropping areas in Korea. We newly found that C. gloeosporioides which does not forming a sexual stage, infects strawberry and differs in some characteristics concerning virulence, cultural and morphological properties to G. cingulata which has a sexual stage. C. gloeosporioides was mainly isolated from the crown with 35.2% rate, while G. cingulata was largely isolated from petiole, runner with 40.9% rate in infected strawberry plants. These two pathogens showed significant differences in cultural characteristics such as perfect stage formation, temperature response as well as benomyl resistance. It was demonstrated that C. gloeosporioides has significantly stronger pathogenicity than G. cingulata in pathogenicity test carried on strawberry plants to various strawberry cultivars. Akihime, Akaneko and Nyoho forcing cultured strawberry cultivars, considered to be susceptible, while semiforcing cultured cultivars, such as Suhong and Holowase, were shown resistant to both pathogens. In non-wound inoculation, C. gloeosporioides was shown pathogenicity on the apple fruit, but G. cingulata could not infect it.

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Touch-induced gene (IbTCH1) from sweet potato [Ipomoea batatas (L.) Lam.]: molecular cloning and functional analysis

  • Seo, Sang-Gyu;Kim, Ji-Seong;Kang, Seung-Won;Shin, Mi-Rae;Yang, You-Sun;Lee, Gung-Pyo;Hong, Jin-Sung;Kim, Sun-Hyung
    • Plant Biotechnology Reports
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    • v.2 no.4
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    • pp.253-258
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    • 2008
  • The cDNA of the touch-induced genes (TCH) of the sweet potato [Ipomoea batatas (L.) Lam.] has been cloned and analyzed. IbTCH1, which exists as at least two-copy genes in the genome of the sweet potato, encodes for 148-amino acid polypeptides, and harbors four conversed $Ca^{2+}-binding$ motif EF-hands. IbTCH1 was shown to be expressed in the flower, leaf, thick pigmented root, and particularly in the white fibrous root, but expressed only weakly in the petiole. IbTCH1 is upregulated upon exposure to environmental stresses, dehydration, and jasmonic acid. Furthermore, IbTCH1 is developmentally regulated in the leaf and root. These results strongly indicate that the gene performs functions in both plant development and in defense/stress-signaling pathways.

Change in the RAPD Band of Strawberry Depending on 2,4-D and NAA Treatment and the Number of Subcultures In Vitro (기내배양에서 2,4-D 및 NAA처리와 계대배양회수에 따른 딸기의 RAPD Band 변화)

  • Shim, Jai-Sung;Chung, Hae-Joon;Min, Byung-Hoon
    • The Journal of Natural Sciences
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    • v.8 no.2
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    • pp.147-152
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    • 1996
  • The 2,4-D + BA combination in MS medium showed high regenerating ability and induced compact callus from leaf and petiole segments of three strawberry cultivars, 'Bokyo', 'Suhong', whereas the NAA + BA combination resulted in friable callus. Band of RAPD products obtained with primer 212 from the callus was different from the band of mother plant when callus induced from leaf segment of 'Suhong' cultivar was maintained in MS medium containing NAA or BA for 8 months. The RAPD bands obtained from mother plants of 'Bokyo' and 'Yeobong' were different from that of callus maintained in the presence of MS liquid medium containing 2,4-D(0.2 mg/$\ell$) subcultured every two weeks for 6 months(12 subcultures).

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Taxonomic reexamination of two Huperzia species (Lycopodiaceae) in Northeast Asia (동북아시아 뱀톱속 (석송과) 두 종의 분류학적 재검토)

  • Lim, Jina;Yun, Na Rae;Lee, Byoung-yoon;Sun, Byung-Yun
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.299-305
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    • 2015
  • A comparison of the external morphology and spores clearly distinguished Huperzia lucidula (Michaux) Trevis. in northeast Asia and North America in terms of leaf shape, leaf margin, distribution of stomata on leaves, and spore shape. Therefore, the northeast Asian plants should be treated as a separate species, H. asiatica (Ching) B.-Y. Sun & J. Lim. In addition, we believe that H. javanica (Sw.) C. Yang, regarded as conspecific or infraspecific with H. serrata (Thunb.) Trevis., is a distinct species based on the presence of a leaf petiole and serrated leaf margins, and the shape of the gemmiferous branchlets.

A taxonomic study of the genus Dioscorea L. (Dioscoreaceae) in Korea based on morphological characters (한국산 마속(마과)의 외부형태형질에 의한 분류학적 연구)

  • Chung, Dae Hee;Chung, Gyu Young
    • Korean Journal of Plant Taxonomy
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    • v.45 no.4
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    • pp.380-390
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    • 2015
  • The morphological characters were observed in six taxa in the genus Dioscorea of Korea (D. polystachya, D. japonica, D. nipponica, D. quinqueloba, D. tenuipes, and D. dokoro). The underground structure and the formation of bulbil were useful characters for separating sect. Enanatiophyllum from sect. Stenophora. The important characters for discriminating taxonomic entities were as follows: the position of the anther and the shape of the pistil in the microstructural characters, including the protuberance of the base of the petiole, the shape and color of the flower, the shape of the fruit, and the shape of the seed wing. Based on the results, a key to Korean Dioscorea is provided and the distribution of these species in Korea is described.

On the Appearance of Boron Deficiency in Sugar Beet, Beta vulgaris L. by the Control of Soil Acidity (토양산도조정에 따른 사탕무우, Beta vulgaris L. 붕소결핍병의 출현에 관하여)

  • 황종서
    • Journal of Plant Biology
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    • v.22 no.1_2
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    • pp.21-27
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    • 1979
  • Sandy loam plots of pH 5.5, which was created by the reclamation of highlands in Yongin county, Kyeonggi province, was selected for the present experimental study. Sugar beet was cultured in these plots by treating them with calcium hydroxide and 3 replications to maintain pH 5.5, 6.4, 6.8, 7.2, and 7.6, respectively. The growth and yield of sugar beet were investigated and the results obtained showed a great deal of boron deficiency. The growth in its early stage was favorable in high pH plots, while no significant difference was observed in sugar content, root weight and sugar production at the time of harvest in each treated plot. The tendency of sugar accumulation appeared to be identical in terms of pattern in each treated plot; i.e. sugar was accumulated until early September but declined momentarily from the middle of September and then the sugar accumulation was resumed from the middle of October. The number of individuals in boron deficiency was larger in high pH plots and the condition of such boron deficiency was also severe. The result of analysis of boron contained in plant body revealed that the boron content of individuals caused boron deficiency by water culture and that occurred in the plots was small alike. The appearance of boron deficiency is characterized by the emergence of black stripes on the surface of the basal part of petiole, severe necrosis of young leaves in the area of growing point and severe wrinkles. Therefore it is felt necessary to pay particular attention to boron deficiency in the culture of sugar beet in our country.

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Comparison of essential oil composition between Angelica gigas and Angelica acutiloba

  • Park, C.H.;Juliani, H.R.;Park, H.W.;Yu, H.S.;Simon, J.E.
    • Plant Resources
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    • v.6 no.3
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    • pp.183-187
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    • 2003
  • Two kinds of Angelica belong to Umbelliferae collected, the one is Angelica gigas that is inhabitant in Korea and the other is Angelica acutiloba that is indigenous in Japan at the field of Snyder Research and Extension Farm Rutgers University, New Jersey and was analyszed by GC and GC/MS. The composition of the essential oil of the different aerial parts of the Angelica has been studied. The oil yields obtained upon hydrodistillation were 0.18% (v/w) in Korean Angelica and 0.44% (v/w) in Japanese Angelica on dry root weight basis. By the growing stage in the Rutgers greenhouse condition, leaf and root of essential oil content a little decreased on 9 months later than 4 months later except for Angelica gigas leaf. Both of Angelica showed that amounts of essential oil content presented in order of leaf > petiole > root according to different plant part. The analysis of the essential oil from Angelica root led to the identification of 14 constituents totaling 64% in Korean Angelica and 13 constituents totaling 68% in Japanese Angelica. The major constituents of the Angelica root essential oil were ligustilide (47 %) and gamma terpi (14 %) in Korean Angelica, and alpha pinei (32 %) and nonane (25 %) in Japanese Angelica

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Frequency and Distribution of Btomate in Korean Ginseng Plant (Pann ginseng C.A. Meyer) (인삼의 기공수분포에 관하여)

  • Lee, Jong-Cheol;Cheon, Seong-Gi;Kim, Yo-Tae
    • Journal of Ginseng Research
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    • v.4 no.1
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    • pp.49-54
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    • 1980
  • This investigation were carrion out to know the frequency, size and distribution of stomata in Korean ginseng, acanthopanax and codonopsis. The results are as follows; 1. Stomatal frequency in ginseng leaf was remarkably less than those of acanthopanax and codonopsis leaf, but size of stomata in ginseng leaf was larger than those of acanthopanax and codonopsis leaf. 2. Stomatal frequency of one year old ginseng plant was higher than those of the older. Two to five years old ginseng plants were not differed in frequency and size of stomata. 3. Frequency and size of stomata were higher and larger in red-berry variant in compare to yellow-berry variant. 4. Stomatal frequency in different leaf Position was not significantly different among those of middle leaf, first side leaf and second side leaf, but in decreasing order of middle Part, upper, lower part and edge in the same ginseng leaf. 5. Stomata was not seen in adaxial surface and petiole of leaf ginseng, acanthopanax and codonopsis. 6. Stomatal frequency was higher in ginseng plant grown under no$.$shading compared to shading, and that of ginseng plant in rear line was less than that of front line under the same shade roof.

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