• Journal of Microbiology and Biotechnology
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• v.27 no.6
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• pp.1171-1179
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• 2017

Butanol is a promising alternative to ethanol and is desirable for use in transportation fuels and additives to gasoline and diesel fuels. Microbial production of butanol is challenging primarily because of its toxicity and low titer of production. Herein, we compared the transcriptome and phenome of wild-type Escherichia coli and its butanol-tolerant evolved strain to understand the global cellular physiology and metabolism responsible for butanol tolerance. When the ancestral butanol-sensitive E. coli was exposed to butanol, gene activities involved in respiratory mechanisms and oxidative stress were highly perturbed. Intriguingly, the evolved butanol-tolerant strain behaved similarly in both the absence and presence of butanol. Among the mutations occurring in the evolved strain, cis-regulatory mutations may be the cause of butanol tolerance. This study provides a foundation for the rational design of the metabolic and regulatory pathways for enhanced biofuel production.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.13 no.1
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• pp.85-93
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• 2000

In this paper, the predischarge propagation processes of SF\ulcorner gas stressed by impulse voltages under nonuniform electric field perturbed by a needle protrusion are described. The statistical and formative time-lags and the time interval between leader pulses were investigated on the basis of the predischarge current measured in the gas pressure range of 0.1~0.5 MPa. The predischarge current is closely related to the waveform, amplitude and polarity of applied votages, the gas pressure and the gap geometry. Both the positive and negative predischarge processes in nonuniform electric field develop in a regime of stepwise leader propagation leading to electrical breakdown. The mean of the time interval between leader pulses gives about a factor of 10 higher for the negative than for the positive leader current puls-es. According as the gas pressure increases, the statistical time-lag was almost unchangeable, but the formative time-lag was gradually decreased.

• Bulletin of the Korean Chemical Society
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• v.31 no.12
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• pp.3521-3524
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• 2010

MTH1880 is a hypothetical protein derived from Methanobacterium thermoautotrophicum, thermophilic methanogen. The solution structure determined by NMR spectroscopy showed that it has a novel $\alpha+\beta$-fold with a highly acidic ligand binding pocket. Since MTH1880 maintains its ultra-stable structural characteristics at both high temperature and pressure, it has been considered as an excellent model for studying protein folding. To initiate the structural and folding study of MTH1880 in proving its unusual stability, we performed the site directed mutagenesis and biochemical analysis of MTH1880 mutants. Data from circular dichroism and NMR spectroscopy suggest that the point mutations perturbed the structural stability of protein even though the secondary structure is retained. This study will provide the useful information in understanding the role of participating residues during folding-unfolding process and our result will be used in designing further folding experiments for hyper-thermopile proteins like MTH1880.

• Nuclear Engineering and Technology
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• v.17 no.4
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• pp.279-289
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• 1985

The purpose of this paper is to investigate the density-wave oscillation type instability in the recirculating loop of U-tube steam generator (UTSG). The perturbed and nodalized conservations equations based on the drift-flux model have been derived to obtain the single-and two-phase pressure drop perturbations, by taking into account the slip between phases, nonuniform heat flux and heated wall dynamics. To assess the stability, the frequency domain technique with the Nyquist criterion has been used under the constant pressure drop boundary condition through the loop. The computer implementation of this model, SASG, was used for the parametric study of the steam generator in Kori-Unit 1. The results of the parametric study revealed important factors influencing UTSG stability margin.

• Journal of Electrical Engineering and Technology
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• v.6 no.5
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• pp.697-705
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• 2011

In this paper, a closed-loop system constructed with a linear plant and nonlinearity in the feedback connection is considered to argue against its planar orbital stability. Through a state space approach, a main result that presents a sufficient stability criterion of the limit cycle predicted by solving the harmonic balance equation is given. Preliminarily, the harmonic balance of the nonlinear feedback loop is assumed to have a solution that determines the characteristics of the limit cycle. Using a state-space approach, the nonlinear loop equation is reformulated into a linear perturbed model through the introduction of a residual operator. By considering a series of transformations, such as a modified eigenstructure decomposition, periodic averaging, change of variables, and coordinate transformation, the stability of the limit cycle can be simply tested via a scalar function and matrix. Finally, the stability criterion is addressed by constructing a composite Lyapunov function of the transformed system.

• The Journal of the Acoustical Society of Korea
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• v.25 no.4
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• pp.172-177
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• 2006

Various research and development on Synthetic Aperture Sonar technique is under way to enhance bearing resolution of a SONAR system. In this paper, we estimated perturbed array shapes, and compensated distortion by using estimated away shapes and synthesized arrays in aperture domain such as an ETAM technique. As experimental data, we used the one obtained from towed array in neighboring waters of the Korean peninsula. Through simulation on data where tow-ship speed is maintained at a constant level, we confirmed that synthesis effect of increasing SNR and narrowed beam width of main lobe was consistently demonstrated for about 1 minute when coherence of target signal was maintained. Also, we showed that the synthesis effect with respect to time was constantly maintained.

• Journal of the Korean Magnetic Resonance Society
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• v.28 no.1
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• pp.1-5
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• 2024

IscU, the iron-sulfur (Fe-S) cluster scaffold protein, is an essential protein for biogenesis of Fe-S clusters. Previous studies showed that IscU manifests a metamorphic structural feature; at least two structural states, namely the structured state (S-state) and the disordered state (D-state), interconverting in a physiological condition, was observed. Moreover, subsequent studies demonstrated that the metamorphic flexibility of IscU is important for its Fe-S cluster assembly activity as well as for an efficient interaction with various partner proteins. Although solution nuclear magnetic resonance (NMR) spectroscopy has been a useful tool to investigate this protein, the detailed molecular mechanism that sustains the structural heterogeneity of IscU is still unclear. To tackle this issue, we applied a high-pressure NMR (HP-NMR) technique to the IscU variant, IscU(I8K), which shows an increased population of the S-state. We found that the equilibrium between the S- and D-state was significantly perturbed by pressure application, and the specific regions of IscU exhibited more sensitivity to pressure than the other regions. Our results provide novel insights to appreciate the dynamic behaviors of IscU and the related versatile functionality.

• Journal of Veterinary Science
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• v.21 no.6
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• pp.91.1-91.15
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• 2020

Background: Sulforaphane (SFN) is an isothiocyanate compound present in cruciferous vegetables. Although the anti-inflammatory effects of SFN have been reported, the precise mechanism related to the inflammatory genes is poorly understood. Objectives: This study examined the relationship between the anti-inflammatory effects of SFN and the differential gene expression pattern in SFN treated ob/ob mice. Methods: Nitric oxide (NO) level was measured using a Griess assay. The inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) expression levels were analyzed by Western blot analysis. Pro-inflammatory cytokines (tumor necrosis factor [TNF]-α, interleukin [IL]-1β, and IL-6) were measured by enzyme-linked immunosorbent assay (ELISA). RNA sequencing analysis was performed to evaluate the differential gene expression in the liver of ob/ob mice. Results: The SFN treatment significantly attenuated the iNOS and COX-2 expression levels and inhibited NO, TNF-α, IL-1β, and IL-6 production in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. RNA sequencing analysis showed that the expression levels of 28 genes related to inflammation were up-regulated (> 2-fold), and six genes were down-regulated (< 0.6-fold) in the control ob/ob mice compared to normal mice. In contrast, the gene expression levels were restored to the normal level by SFN. The protein-protein interaction (PPI) network showed that chemokine ligand (Cxcl14, Ccl1, Ccl3, Ccl4, Ccl17) and chemokine receptor (Ccr3, Cxcr1, Ccr10) were located in close proximity and formed a "functional cluster" in the middle of the network. Conclusions: The overall results suggest that SFN has a potent anti-inflammatory effect by normalizing the expression levels of the genes related to inflammation that were perturbed in ob/ob mice.

• Transactions of the Korean Society of Mechanical Engineers A
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• v.40 no.4
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• pp.381-387
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• 2016

In this paper, the methodology applied to the improvement of stress analyses is extended to free vibration and buckling analyses. The essence of the methodology is the Saint-Venant's principle that is applicable to beam and plate models. The principle allows one to dimensionally reduce three-dimensional elasticity problems. Thus the methodology can be employed to vibration and buckling as well as stress analysis. First, the principle is briefly revisited, and then the formations of classical beam theories are presented. To improve the predictions, the perturbed terms (unknowns) are introduced together with the warping functions that are calculated by stress equilibrium equations. The unknowns are then calculated by applying the equivalence of stress resultants (i.e., Saint-Venant's principle). As numerical examples, cantilever and simply supported beams are analytically solved. The results obtained are compared with those of the classical beam theories. It is shown that the methodology can be used to improve the predictions without introducing shear correction factors.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.15 no.3 s.96
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• pp.251-258
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• 2005

The free vibration of a spinning flexible disk-spindle system supported by hydro dynamic bearings (HDB) in an HDD is analyzed by FEM. The spinning flexible disk is described using Kirchhoff plate theory and von Karman non-linear strain, and its rigid body motion is also considered. It is discretized by annular sector element. The rotating spindle which includes the clamp, hub, permanent magnet and yoke, is modeled by Timoshenko beam including the gyroscopic effect. The flexible supporting structure with a complex shape which includes stator core, housing, base plate, sleeve and thrust pad is modeled by using a 4-node tetrahedron element with rotational degrees of freedom to satisfy the geometric compatibility. The dynamic coefficients of HDB are calculated from the HDB analysis program, which solves the perturbed Reynolds equation using FEM. Introducing the virtual nodes and the rigid link constraints defined in the center of HDB, beam elements of the shaft are connected to the solid elements of the sleeve and thrust pad through the spring and damper element. The global matrix equation obtained by assembling the finite element equations of each substructure is transformed to the state-space matrix-vector equation, and the associated eigen value problem is solved by using the restarted Arnoldi iteration method. The validity of this research is verified by comparing the numerical results of the natural frequencies with the experimental ones. Also the effect of supporting structures to the natural modes of the total HDD system is rigorously analyzed.