• Journal of Life Science
• /
• v.21 no.7
• /
• pp.976-984
• /
• 2011

Total phenolic content was the highest in 60% ethanol extracts at 21.91 mg/g, and inhibitory activity against tyrosinase of 60% ethanol extracts was higher than ethanol extracts of other concentration. The inhibitory compounds against tyrosinase from Persimmon leaves were purified using Sephadex LH-20, MCI-gel CHP-20 column chromatography with gradient elution. Two purified compounds were isolated as a result. The chemical structures of each compound were determined and identified using $^1H$-NMR and $^{13}C$-NMR, FAB-Mass. The compounds were confirmed as (+)-gallocatechin and prodelphinidin B-3. The tyrosinase inhibitory activities of purified (+)-gallocatechin and prodelphinidin B-3 were 29.5 and 40.2%, respectively. The inhibitory activities of (+)-gallocatechin and prodelphinidin B-3 against melanin biosynthesis in melanoma cell were 32.5 and 46.7%. The safety of essence with tyrosinase inhibitory compounds from persimmon leaves was also assessed by various safety profiles. First, changes in pH (4.90~4.95) and viscosity (23,000~26,000 cP) was not detected for 60 days. Essence also showed stability against temperature and light for 60 days. All these findings suggest that extracts from persimmon leaves have a great potential as a cosmetical ingredient with a potent whitening effect.

• Plant Disease and Agriculture
• /
• v.5 no.1
• /
• pp.55-57
• /
• 1999

Botrytis cinerea was isolated from a gray mo이 leaf lesion on persimmon in fields of Kyeonsangnam-do from 1996 to 1998, and etiological study was conducted including physiological characteristics. It formed gray mold lesion with light green color on leaves of persimmon (Diospyros kaki). The temperature range for mycelial growth was between $5^{\circ}C$ and $30^{\circ}C$ with the optimum temperatures of $20^{\circ}C$ to $25^{\circ}C$. Conidia were oviod, cylindric, and colorless and their dimensions in culture were $8.4~11.5\times7.0~8.9\mu\textrm{m}$. The optimum temperature of conidial germination was $25^{\circ}C$. Sclerotia on potato dextrose agar medium were well formed and brownish condiophores were observed with their size of $18.5~64.9\times4.5~8.0\mu\textrm{m}$. Symptoms on artificially inoculated plants were similar to those of gray mold disease on persimmon caused by Botrytis cinerea in fields.

• Plant Disease and Agriculture
• /
• v.1 no.2
• /
• pp.18-21
• /
• 1995

Mycosphaerella nawae, the causal organism, of spotted leaf casing disease of persimmon, was isolated from infected leaves showing typical symptom. The cultural characteristics of the fungus were compared on artificial media. Among 24 different combinations of culture media and supplements, oatmeal agar+persimmon leaf extract (PLE) and PAD+ PLE+streptomycin showed the highest rate of isolating as 57.1%. The best medium for mycelial growth of the fungus was PDA+persimmon leaf powder (PLP). The colony diameter was reached 47mm for 30 days at 2$0^{\circ}C$. PDA+PLE also supported good mcyelial growth showing 46mm of diameter in same condition. The optimum growth temperature of this fungus in PDA was recognized fairly low. The mycelial growth was higher at 2$0^{\circ}C$ than 15$^{\circ}C$. The variation of pH between pH 4 to pH 8 did not affect to the mycelial growth of the pathogen.

• Korean Journal of Soil Science and Fertilizer
• /
• v.46 no.4
• /
• pp.276-280
• /
• 2013

D. kaki and D. lotus are used as rootstocks for astringent persimmons in Korea but characteristics of their seedlings have not been determined. In this experiment, their seeds were sown in 3-L pots on April 18 and the seedlings were grown until October 24. Growth and nutrient absorption were compared at the end of the season after destructively harvesting the seedlings. Seedling growth of D. lotus was much faster than that of D. kaki in terms of total stem length, stem diameter, and number of leaves. However, chlorophyll value and specific leaf weight were higher in D. kaki than in D. lotus. Dry weight of D. lotus was 3.6- and 3.7-fold higher than that of D. kaki in above-ground parts and the root, respectively. D. kaki seedlings were characterized by higher concentrations of N, P, K, Ca, and Mg in the leaves, stem, or the root. However, total contents of the elements were 1.8- to 3.7-fold higher in a D. lotus seedling due to its greater dry weight. Since D. lotus seedlings absorbed more inorganic elements on a tree basis and grew more vigorously than D. kaki seedlings, the level of fertilization for astringent persimmons should be adjusted depending on rootstocks to maintain the trees at the optimum vigor.

• Korean Journal of Soil Science and Fertilizer
• /
• v.15 no.4
• /
• pp.258-269
• /
• 1982

The nutritional status of fruit leaves of sweet persimmon and soil conditions were observed in the fifty five blocks of fruit farms of sweet persimmon in Gimhae, Gyeong-nam area. 1. The soil of fruit farm was composed of more than 20% of gravel in the most part of the fruit farms. 2. The strong acidity was observed in the soil of young fruit farms but the acidity of the farm soils was improved to week acid with increase of age of fruit trees. 3. The great variation in the content of available phosphate were observed in the soil from different fruit farm blocks and the lime content were relatively low in the most of the farm soils. 4. The contents of nitrogen, phosphate and potassium in leaves of sweet persimmon collected in July and October were lower than those collected in May but reverse tendency was observed in the content of calcium, magnesium and sodium. 5. The content of boron in leaves collected in May was similar to that collected in July but extremely high content of boron was observed in the loaves collected in October, harvesting period of sweet persimmon. 6. The contents of iron and manganese in the leaves of sweet persimmon were observed to be relatively high compared to the content in the leaves of other fruit trees. 7. The relatively great variation in such inorganic components as iron, copper and boron was observed in the fruit leaves of sweet persimmon according to the different fruit farm blocks. 8. The ages of fruit trees didn't have an effect on the content of inorganic components in the fruit leaves except the content of nitrogen which was decreased with the increase of the ages of fruit trees.

• Korean Journal of Pharmacognosy
• /
• v.41 no.4
• /
• pp.255-263
• /
• 2010

Kojongsi persimmon (Diospyros kaki) is the major cultivar of astringent persimmon in southern of Korea. Kojongsi persimmon leaf has been traditionally used for acute and chronic diseases in Oriental countries. The purpose of this study was undertaken to investigate the antioxidative activities of the extract of Kojongsi persimmon leaf during growth. We investigated the antioxidant effects of the persimmon leaf extracts during growth on total polyphenol, total flavonoid, electronic donating ability (DPPH), nitrite (NO) scavenging and superoxide dismutase (SOD)-like activity. The next, we investigated the possible cell protective effects of the persimmon extract treatment against ultraviolet B (UVB)-induced injury in HaCaT keratinocytes. The contents of total polyphenol and flavonoid in leaf extract of Kojongsi persimmon were increased in time-dependent manner. In Jun, DPPH and NO radical scavenging and SOD-like activities in the leaf extract of Kojongsi persimmon was increased to the highest. However, the antioxidant activities in persimmon varieties were not any difference. The cell cytotoxicity by UVB irradation in HaCaT keratinocytes was significantly increased with the compared to the control group. However, the treatment of leaf extract of Kojongsi persimmon in HaCaT keratinocytes was shown to protective effect against UVB-induced cell cytotoxicity. These results suggest that the leaf extract of Kojongsi persimmon has potent antioxidant activity, and protective effect against UVB-induced keratinocyte injury. Thus, these properties may be contributed in the care of acute and chronic diseases.

• Korean journal of applied entomology
• /
• v.41 no.3
• /
• pp.183-189
• /
• 2002

Occurrence of scarabs at sweet persimmon (Diospyros kaki var. Fuyu) orchards was investigated by mercury light traps every one week interval in several areas in Gyeongnam province including, Jinju, Sacheon, Sancheong, and Gimhae, from April to September in 2000 and 2001. In addition, damage of persimmon by scarabs was observed every ten days interval at three orchards in Jinju and at one in Gimhae from late May to late October. Although sixteen species of 12 genera were attracted to the traps, species and number of catches were different according to orchards and years. Holotrichia morosa was most dominant in Jinju, Sacheon, and Sancheong. Total number of scarabs attracted to the traps was highest at the orchard surrounded by chestnut orchards in Sancheong. Fruits of sweet persimmon were not damaged by scarabs at the studied orchards. However, leaves and calyxes were slightly damaged by Adoretus tenuimaculatus. Maximum average numbers of the damaged leaves and calyxes throughout the year by A. tenuimaculatus were 0.33 leaves from 10 new shoots and 0.07 calyxes from 15 fruits. Gametis jucunda and Popillia mutans damaged flowers and calyxes. Maximum average numbers of damaged flowers and calyxes by these 2 species were the same as 0.03 from 15 flowers and 15 calyxes, respectively. These levels of damage suggest that the scarabs are not economically injurious to sweet persimmon fruits in Korea.

• Proceedings of the Costume Culture Conference
• /
• 2005.10a
• /
• pp.127-131
• /
• 2005

• Korean Journal of Food Science and Technology
• /
• v.30 no.3
• /
• pp.529-534
• /
• 1998

A functional and taste related composition was determined to investigate the possibilites of developing a highly functional beverage from Chungdo Bansi, Sangju Dungsi and Byue during growth. Moisture content decrease continously from 77.9% to 80.1% at the begining of May during growth. Crude protein and fat contents in the three species gradually increased according to growth until August. There ware four sorts of free sugar components which were sucrose, glucose, fructose and raffinose in the three species. Buyu's sucrose content was each 1378, 143 times higher than Chungdo Bansi and Sangiu Dungsi by the standard of July. Vitamin C content in the three species gradually increased form the flowering time and was the highest content in July. The sorts of free amino acids in persimmon leaves were detected eighteen free amino acids in all three species. As a result, the materials of persimmon leaves exhibited the highest component from June to July and Buyu of them excelled in a lot of composition.

• Korean Journal of Food Science and Technology
• /
• v.52 no.4
• /
• pp.363-368
• /
• 2020

This study was conducted to evaluate several physiological activities of 4 sweet persimmon cultivar leaf water extracts. One new cultivar ('Hongchu' (HC)]) and three traditional cultivars ('Sangseojosaeng' (UW), Japanese Uenishiwase; 'Seochonjosaeng' (NW), Japanese Nishimurawase; and 'Buyu' (FY), Japanese Fuyu) were used in this study. The HC extract showed significantly higher DPPH and ABTS radical scavenging activities, tyrosinase inhibitory activity, and acetaldehyde dehydrogenase activity than the other extracts, while the FY extract exhibited a relatively higher alcohol dehydrogenase activity. The HC extract contained higher amount of phenolics and ascorbic acid. These results suggest the possibility of high-quality persimmon leaf tea development using the new sweet persimmon cultivar, HC.