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http://dx.doi.org/10.5352/JLS.2011.21.7.976

Application of Isolated Tyrosinase Inhibitory Compounds from Persimmon Leaves  

Cho, Young-Je (School of Food Science & Biotechnology, Food & Bio-Industry Research Institute, Kyungpook National University)
An, Bong-Jeun (Department of Cosmeceutical, Daegu Hanny University)
Kim, Jeung-Hoan (School of Nano Materials Engineering, Kyungpook National University)
Publication Information
Journal of Life Science / v.21, no.7, 2011 , pp. 976-984 More about this Journal
Abstract
Total phenolic content was the highest in 60% ethanol extracts at 21.91 mg/g, and inhibitory activity against tyrosinase of 60% ethanol extracts was higher than ethanol extracts of other concentration. The inhibitory compounds against tyrosinase from Persimmon leaves were purified using Sephadex LH-20, MCI-gel CHP-20 column chromatography with gradient elution. Two purified compounds were isolated as a result. The chemical structures of each compound were determined and identified using $^1H$-NMR and $^{13}C$-NMR, FAB-Mass. The compounds were confirmed as (+)-gallocatechin and prodelphinidin B-3. The tyrosinase inhibitory activities of purified (+)-gallocatechin and prodelphinidin B-3 were 29.5 and 40.2%, respectively. The inhibitory activities of (+)-gallocatechin and prodelphinidin B-3 against melanin biosynthesis in melanoma cell were 32.5 and 46.7%. The safety of essence with tyrosinase inhibitory compounds from persimmon leaves was also assessed by various safety profiles. First, changes in pH (4.90~4.95) and viscosity (23,000~26,000 cP) was not detected for 60 days. Essence also showed stability against temperature and light for 60 days. All these findings suggest that extracts from persimmon leaves have a great potential as a cosmetical ingredient with a potent whitening effect.
Keywords
Application; tyrosinase inhibition; phenolics; persimmon leaves;
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