The characteristics of alcohol fermentation using sweet persimmon juice were studied in static fermentation in an effort to develop new types of functional wine. The yeast strain Saccharomyces cerevisiae KCCM 12650 was selected for use in the fermentation of sweet persimmon juice. Attempts were made to modify the sweet persimmon juice in order to find suitable conditions for alcohol fermentation. The modified sweet persimmon juice (pH 4.0) that was most suitable for alcohol fermentation contained $24^{\circ}Brix$ of sugar supplemented with sucrose as a carbon source and 0.5 g/L of $(NH_4)_2HPO_4$ as a nitrogen source. After 5 days of fermentation at $25^{\circ}C$, 12.8% of alcohol was produced from the modified juice and its pH was slightly decreased to 3.9. Browning of the wine was observed during storage due to the oxidation of phenolic compounds. The initial browning of 0.08% at $OD_{420}$ after fermentation increased to 0.40 during storage for 11 weeks at room temperature. The addition of $K_2S_2O_5$ was effective in delaying the browning of the wine. The browning of the wine decreased to 0.25 at $OD_{420}$ with the addition of 200 mg/L of $K_2S_2O_5$. The wine produced in this study contained some organic acids such as malic acid (6.82% g/L) and succinic acid (1.40 g/L), some minerals such as $K^+$ (947.8 mg/L) and $Mg^{2+}$ (36.4 mg/L), as well as soluble phenolics (779 mg/L of gallic acid equivalent). Schisandra fruit was added to the sweet persimmon juice during alcohol fermentation in order to improve the sour taste and flavor. The best sensory quality (taste, flavor, and color) was obtained by adding 0.5% schisandra fruit.
This paper was carried out to investigate changes in chromatograms of polysacctatides and soluble pectins on Sephadex G-50 and non-cellulosic neutral sugars of polysaccharides isolated from cell wall of persimmon fruits treated with polygalacturonase and $\beta$-galactosidase in vitro. The chromatogram pattern of soluble pectins extracted from cell wall treated with $\beta$-galactosidase on Sephacryl S-500 column were similar to those of untreatment, but contents of soluble pectins treated with $\beta$-galactosidase were different from those of untreatment. The patterns of chromatograms In soluble pectins extracted from cell wall treated with polygalacturonase were more complex and lower molecular polymer than those of other cell wall-degrading enzyme treatments. Non-cellulosic neutral sugar of polysaccharides in fraction I of soluble material treated with polygalacturonase was rhamnose, those in fraction II were similar to those in fraction III and contents of arabinose, xylose and glucose were higher than contents of other non-cellulosic neutral sugars. Non-cellulosic neutral sugars of polysaccharides in fraction I in soluble material by $\beta$-galactosidase treatment were rhamnose, arabinose, galactose and mannose. Content of glucose of polysaccharides in fraction II was higher than that in fraction I . Non-cellulosic neutral sugars treated with mixed enzyme were rhamnose, fucose, arabinose, xylose, mannose, galactose and glucose. Compositions of non-cellulosic neutral sugars of polysaccharides in fraction I were similar to those in fraction II and III.
Persimmon wood (Diospyros kaki) is a seasonal fruit-producing plant with a beautiful dark pattern in its wood that is suitable for high-quality furniture, sculptures and musical instruments. The utilization of persimmon wood can be improved by determining its anatomical characteristics, such as juvenile and mature wood. This study aimed to determine the boundaries between juvenile and mature wood and observe the anatomical properties of juvenile and mature wood and their variations in the axial direction. Three 30-year-old persimmon (D. kaki) trees grown in Karo, North Sumatra, Indonesia, were used in this study. The boundary between juvenile and mature wood was determined by measuring the fiber length and vessel element length from near the pith to near the bark. Anatomical observations were conducted in the juvenile and mature wood areas. The results showed that the average boundaries between juvenile and mature wood were 44.11 mm from the pith and were not significantly different in the axial direction of the trees. Furthermore, the wood anatomy categories of juvenile and mature wood differed significantly in terms of fiber diameter, fiber proportion, vessel proportion, and axial parenchyma proportion. In the axial direction, vessel diameter, ray parenchyma frequency, and ray parenchyma proportion at the base, middle, and top of the tree were significantly different.
To investigate main air temperature factors correlated to difference of fruit characteristics according to cultivating areas, fruit and air temperature characteristics of eight cultivating areas of 'Fuyu' persimmon were analyzed by principle components and multiple regression analysis. The first principal components extracted from 16 air temperature factors was annual mean temperature, mean temperature during October, annual mean minimum extreme temperature, mean temperature during growing period, and so forth. The second principal components was mean temperature during May and June and so forth. And cumulative contribution was 91.4%. The five of eight cultivating area had clearly the difference of main factors or the correlated direction among cultivating areas. In multiple regression analysis between the extracted main factors and fruit characteristics, fruit hight were highly correlated with mean temperature during growing period ($X_8$) and cumulative temperature ($X_6$), and the regression equation was $Y=150.55-5.375X_8+ 0.014X_6(r^2=0.843)$. Also this regression equation was affected by mean minimum temperature during growing period, cumulative temperature, and mean temperature during August. Fruit diameter was negatively correlated with mean temperature during growing period, flesh browning rate and Hunter a value of peel color were positively correlated with mean minimum temperature during growing period and annual minimum air temperature, respectively.
Lowering tree height has been a key practice for efficient management of persimmon orchards. This experiment was conducted to assess whether fertilization rates could be reduced after lowering the tree height by heavy pruning. Twelve-year-old 'Uenishiwase' persimmon trees were lowered to 2 m from over 3 m by severe dormant pruning, and then conventional fertilization rate was applied to some trees in April (N 224 g, $P_2O_5$ 172 g, and $K_2O$ 172 g per tree), June (N 112 g and $K_2O$ 99 g per tree), and October (N 112 g and $K_2O$ 99 g per tree). At the same time, 1/3 or 2/3 of the conventional rate and none were applied to other trees, respectively. Non-application decreased shoot length and reduced number of unnecessary secondary shoots by 39% compared with the conventional rate, not affecting yield and weight, color, firmness, and soluble solids of fruits. No significant difference was also found in the yield and the fruit characteristics among the trees fertilized with different rates. Concentrations of soluble sugars, starch, N, and K of dormant shoots in March of the following year were not significantly changed by the different treatments of the previous year. There was no significant difference of shoot growth and yield among the treatments the following year when the same fertilization rate was supplied to all the trees. Results indicated that fertilization rate could be reduced to less than 1/3 of conventional rate to save the cost and stabilize shoot vigor when tree height is lowered by severe pruning.
A series of study has been conducted to develop a multi-purpose fruit grader using a black & white image processing system equipped with a 550 nm interference filter. A device and high performance algorithms were developed for sizing and color grading of Fuji apple in the previous study. In this study an emphasis was put on finding correlations between weights of several kinds of fruits and their area fractions(AF), and on compensating the blurring effect upon sizing and color grading by conveying speed of fruit. Also, the effect of orientation and direction of fruit on conveyor during image forming was analyzed to identify any difficulty (or utilizing an automatic fruit feeder. The results are summarized as follows. 1. The correlation coefficients(r) between the weights of fruits and their image sizes were 0.984~0.996 for apples, 0.983~0.990 for peachs, 0.995 for tomato, 0.986 for sweet persimmon and 0.970~0.993 for pears. 2. It was possible to grade fruits by color with the area weighted mean gray values(AWMGV) based on the mean gray valves of direct image and the compensated values of reflected image of a fruit, and also possible to sort fruits by size with AF. Accuracies in sizing and color grading ranged over 81.0% ~95.0% and 82.0% ~89.7% respectively as compared with results from sizing by electronic weight scale and grading by expert. 3. The blurring effect on the sizing and color grading depending on conveying speed was identified and regression equations were derived. 4. It was found that errors in sizing and coloring grading due to the change in direction and orientation of Fuji apple on the conveyor were not significant as far as the stem end of apple keeping upward.
Persimmon (Diospyros kaki. cv. Fuyu) fruits were packaged under different conditions, and then stored at $0^{\circ}C$ for 21 weeks. The tried packages were heat-sealed bag of one fruit, heat-sealed bag of five fruits, and tie-sealed bag of five fruits, which used films of three different thickness (0.03 ㎜, 0.05 ㎜ and 0.06 ㎜). Throughout the storage, package free volume, package atmosphere and quality were measured. Package free volume decreased with time with higher rate for heat-sealed bags, in which close contact between fruit and the film was eventually reached in longer storage. However, tie-sealed bags maintained the levels of stabilized free volume. The rate of free volume decrease was faster with thinner film and larger bag size for the packs sealed by the same method. Package atmosphere covered $O_2$ concentration of $1.1{\sim}17.1%$ and $CO_2$ concentration of $1.1{\sim}8.3%$, $O_2$ concentration decreased during storage, whereas $CO_2$ increased. Thinner film package created the internal atmosphere of higher $O_2$ and lower $CO_2$ concentrations. Tie-sealed bags of 5 fruits in the films of 0.05 mm and 0.06 mm thickness maintained the equilibrated package atmosphere of $1.1{\sim}3.0%\;O_2$ and $4.0{\sim}5.5%\;CO_2$, which preserved the fruits best in terms of firmness and less physiological changes of flesh browning and peel browning. At five fruit heat-sealed bag in the films of 0.06 mm thickness with $CO_2\;8.3%$, flesh browning occurred at a high rate, whereas all treatments in the film of 0.03 mm thickness with high $O_2$ and relatively low $CO_2$ contents, flesh and peel browning rates were high.
The effect of storage temperatures ($0^{\circ}C$and $20^{\circ}C$) and packing functional film for fruit quality were examined in 'Fuyu' persimmon. The fresh weight loss was delay more effectively by the inhibition of transpiration when functional film was used. The firmness was effectively maintained in functional film packages at low temperature ($0^{\circ}C$), which might result from the beneficial combination of $CO_2$ and $O_2$. The soluble solid contents was higher with packing of functional film at $0^{\circ}C$ than at room temperature ($20^{\circ}C$). Highest $CO_2$ production in functional film bags was observed in room temperature storage after one week and thereafter declined sharply whereas lowest in 8 weeks. $CO_2$ concentration regardless of packing slowly increased to $5.2{\mu}L{\sim}10.2mL{\cdot}kg^{-1}{\cdot}h^{-1}$ until 8 weeks of low temperature due to persimmon respiration. Hunter 'b' value changed greatly yellowness according to temperature of storage but there were no significant differences among packaging treatments. Effects of functional film and low temperature in slower softening were consistent. This results suggested that the packing functional film at low temperature resulted in the best retention of quality market.
Journal of the Korean Applied Science and Technology
/
v.34
no.3
/
pp.478-486
/
2017
To find the effect of packaging materials(LDPE $60{\mu}m$, Ceramic $30{\mu}m$ and $60{\mu}m$) to enhance the shelf-life of de-astringent persimmon, the fruits used were the astringent persimmons of "Cheongdobansi" and astringency was removed in $CO_2$. De-astringent persimmon were stored in MA for 140 days at $5^{\circ}C$. During storage, the concentration of oxygen was decreased to 5.2%, while the concentration of carbon dioxide was increased to 5.2% from 18 days. But the concentration of oxygen and carbon dioxide was maintained at a constant concentration until the end of storage. The rate of weight loss was gradually decreases for the storage time elapsed and has better in ceramic $60{\mu}m$ packaging than other packaging materials. Fruit hardness and color value were well maintained in ceramic $60{\mu}m$ packaging. Tannin content and soluble solid were not significant difference among the packaging conditions. Ethanol and acetaldehyde content were decreased during early period of storage and then increased. As a result of objective analysis, de-astringent persimmon storage in ceramic $60{\mu}m$ was more effective than other storage conditions.
A fruit rot of sweet persimmon(Diospyros kaki cv. 'Fuyu') that infected with blue mold was found during the storage and transport in Jinju Gyeongnam Province, Korea. Fruit surfaces that infected with the fungus were formed water soaked lesion at first then gradually colonized with the fungus and formed mycelial mats. From the point of infection, fruits become sunken and mostly ruptured. The pathogenic fungus was isolated from infected fruits and cultured on potato dextrose agar. The colonies of the pathogenic fungi were white at frist then became greyish green on malt extract agar. Conidia were ellipsoidal and $2.6{\sim}3.8{\times}2.4{\sim}3.8{\mu}m$ in size. Phialides were ampulliform, verticilate of 3-7, $8.0{\sim}9.2{\times}2.0{\sim}3.0{\mu}m$ in size. Metulae were verticils of 2-4, smooth, $9.0{\sim}12.6{\times}3.0{\sim}4.6{\mu}m$ in size. Ramuli were groups 1-3, smooth, $11.0{\sim}17.6{\times}2.3{\sim}3.0{\mu}m$ in size. Rami were groups 1-2, $7.5{\sim}32.6{\times}2.6{\sim}4.2{\mu}m$ in size. Stipes were septate, smooth, thin walled, $56{\sim}302{\times}2.8{\sim}4.0{\mu}m$ in size. Penicilli were mostly quaterverticillate. Based on the cultural and mycological characteristics as well as pathogenicity test on host plants, the fungus was identified as Penicillium expansum. This is the first report on the blue mold of sweet persimmon(Diospyros kaki) caused by P. expansum in Korea.
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