• Journal of Korean Neurosurgical Society
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• v.59 no.6
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• pp.643-646
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• 2016

Chordoid glioma of the third ventricle is a rare and challenging tumor to surgery because of its unique anatomical location and its close juxtaposition to the neurovascular structures and hypothalamus. The authors report a case of chordoid glioma of the third ventricle in a 43-year-old woman, who presented with headache and somnolence. The tumor was approached by endoscopic transnasal technique with a favorable result. Histopathologic examination disclosed a neoplastic tissue composed of eosinophilic epithelioid cells, mucinous, periodic acid Schiff-diastase positive, extracellular matrix, and scattered lymphoplasmacytic infiltrates. The best treatment option remains controversial. Customarily, the surgical route to remove chordoid glioma is transcranial; however, the undersurface of the optic chiasm and optic nerves preclude an adequate surgical visualization. In contrast, an expanded endoscopic transnasal approach provides a direct midline corridor to this region without any brain retraction.

• Journal of Veterinary Clinics
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• v.23 no.4
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• pp.472-475
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• 2006

Aspegillosis in free-living birds can be occurred mostly under poor weather and climate. But, captive birds tend to be more susceptible to infection and diagnosis was made usually at post mortem. A two months old Whooper swan(Cygnus c. cygmus) dying suddenly was found in a zoo without prior clinical signs. At necropsy, numerous well- demarcated yellow to white firm nodules were scattered throughout the air sacs and the lungs. Microscopically, granuloma formations were observed in the lung and air sacs. The margin of granuloma was surrounded by connective tissue barrier and was infiltrated with lymphocytes, and also observed giant cell near the granuloma. By Periodic acid Schiff reaction, hyphae were detected in granuloma of lung and air sacs. This case was diagnosed as an invasive pulmonary aspergillosis caused by Aspergillus fumigatus infection in a Whooper swan at a zoo.

• Korean Journal of Veterinary Research
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• v.26 no.1
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• pp.125-137
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• 1986

Sprague-Dawley rats received subcutaneous injections of gentamicin sulfate, 50 and 100mg/kg for 3, 7 and 10 days. The hematological and blood chemical values were determined. Kidneys were examined histologically and ultrastructurally. The results obtained were summarized as follows; 1. The serum values of aspartate transaminase, blood urea nitrogen and creatinine in the rats administered gentamicin, 50 and 100mg/kg/day were significantly increased than those in the control. 2. The ratio of kidney weight to body weight was significantly increased in the rats injected gentamicin, 100mg/kg for 10 days than those in the control. 3. The brush borders of proximal convoluted tubules in the kindneys received gentamicin, 50 and 100mg/kg/day were decreased or absent in periodic acid-Schiff staining. 4. The necrosis of proximal convoluted tubules was shown in the rats given gentamicin, 50 and 100mg/kg for 7 and 10 days. 5. The regeneration of the proximal tubular epithelia was observed in the rats treated gentamicin, 100mg/kg for 10 days. 6. The number and size of lysosomes were increased in the proximal convoluted tubules of the rats injected gentamicin, 50mg/kg for 7 days, many of which contained myeloid bodies.

• The korean journal of orthodontics
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• v.11 no.2
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• pp.57-64
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• 1981

The purpose of this study was to investigate the effect of hydrocortisone in the fusion mechanism of the secondary palatine shelves in the rat. Pregnant rats were injected with 5.0 mg/100 gm body weight of hydrocortisone for 3 days between the 7th day and the 9th day of pregnancy. Between the 14th day md the 18 day of pregnancy, the fetuses were removed and decapitated to be immersed in $10\%$ formalin and Carney's solution. Preparations were stained with alizarin red S, hematoxylin-eosin and alcian blue respectively, and partly were treated for Periodic acid-Schiff reaction. The results were as followings: 1. The incomplete fusion of the palatal epithelium took place though the polarity of epithelial cells. 2. At the edge of shelves the differentiation of mesenchymal cells was observed, but the inter penetration of mesenchyme was not shown. 3. It was considered that the phenomenon of hypocalcification in matrix had relation to the decrease stainability to alizarin red S in shelves. 4. It might be concluded that the connective tissue under epithelium showed the decrease tendency to alcian blue and PAS reaction due to the inhibition of the synthesis of the mucopolysaccharide.

• Animal cells and systems
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• v.11 no.2
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• pp.183-189
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• 2007

To investigate the properties of mucosubstances of the epidermis in various teleostean species, conventional histochemical stainings were used on the skin in five species of order Perciformes, i. e., yellowtail, Seriota quinqueradiat, striped beakperch, Oplegnathus fasciatus, brown spotted grouper, Epinephelus chlorostigma, sea chub, Ditrema temmincki and multicolorfin rainbowfish, Halichoeres poecilopterus. The following methods were used: periodic acid Schiff (PAS), alcian blue (AB) pH at 2.5, AB pH at 1.0, AB pH at 2.5-PAS, AB pH at 1.0-PAS, aldehyde fuchsin (AF) pH at 1.7-AB pH at 2.5 and high iron diamine (HID)-AB pH at 2.5. The epidermis of all five species consisted of three layers: superficial, middle, and basal layer. The superficial layer was comprised of rather flattened cells. In particular, the outermost layer of striped beakperch and middle layer of sea chub consisted of mucus-secreting cells. Mucous cells, the unicellular glands, were found in epidermis but varied in number in different body regions and species. Although there was a slight difference in the amount in various species and body regions, the secretory contents of the mucous cells in the five teleostean species contained acidic mucopolysaccharides. In yellowtail, striped beakperch, and multicolorfin rainbowfish, the property of mucosubstances was identified as sialomucin, while it was sulphomucin in brown spotted grouper and sea chub.

• The Korean Journal of Cytopathology
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• v.6 no.2
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• pp.204-208
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• 1995

The cytologic findings of endodermal sinus tumor of the ovary are reported. The cytologic preperations were obtained from ascitic specimens. The findings on Papanicolaou-stained smears included a clean background and poorly preserved atypical cells loosely arranged in irregular or papillary groups. At high magnification, the cells had ill-defined and microvacuolated cytoplasms, with an increased nuclear-cytoplasmic ratio and prominent nucleoli. Hyaline globules characteristic of the alpha-fetoprotein (AFP)-synthesizing cells of endodermal sinus tumor were observed within the cells with periodic-acid-Schiff(PAS) stain. The presence of PAS-positive hyaline globules can be regarded, therefore, as a diagnostic clue to endodermal sinus tumor in an appropriate clinical setting and in the presence of AFP production. The cytologic findings in a cell block is important, in addition to smears, to obtain more diagnostic clues. A preliminary cytomorphologic diagnosis of this highly malignant tumor is valuable as a guide in planning further immunocytochemical and serologic studies.

• Journal of Physiology & Pathology in Korean Medicine
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• v.17 no.5
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• pp.1276-1280
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• 2003

To investigate the effect of acupuncture at Zushanli (ST 36) in this study, gastric endocrine cells (G cell) by avidin-biotinylated complex (ABC) technique and histological examinations (HE; periodic acid schiff, PAS; alcian blue stain) of the stomach were perfomed at 1, 3, 6 weeks in normal rats. In other groups, omeprazole were fed for 1, 3, 6 weeks to compare with acupuncture effect. Acupuncture applied to the ST 36 acupoint and the administration of omeprazole increased G cell significantly at 1, 3, 6 weeks in time dependant manner. Furthermore, acupuncture applied to the other acupoint on GB 34 did not produce significant effect. When the common peronial nerve was dissected, acupuncture of ST 36 acupoint produced change of G cell. These data suggest that acupuncture at ST 36 increased G cell in point specific way and that effect was not related with surrounding nerve.

• Korean Journal of Veterinary Service
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• v.27 no.1
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• pp.53-62
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• 2004

The present study was carried out to investigate the prevalence of Neospora caninum infection in Korean indigenous cattle and calves of abnormal deliveries and focus on correlation between malformation and N caninum infection. To determine the prevalence of antibodies to N caninum, sera of 473 Korean indigenous cattle from slaughter house were tested for N caninum antibodies using indirect fluorescence assay. Of the 473 cattle sera, 9.5% (45/473) showed positive against N caninum. Regional seropositive rates of the samples were 16.7% (5/85), 11.0% (11/100), 8.8% (21/240) and 5.9% (5/85) at Kyonggi, Gyeongbuk, Daegu and Kyongnam province, respectively. In female, seropositive rates were 17.5% (25/143) and 6.1% (20/330) in male. During the period from march 2000 to August 2001, 55 abnormal deliveries of Korean indigenous cattle including abortion, stillbirth and congenital malformation were examined by histopathological, immunohistochemical and serological methods for evidence of N caninum infection. Of the 55 abnormal deliveries, only 5 calves showed positive reaction against N caninum in serological test. In microscopical observation, gliosis and nonsuppurative myositis were observed. However, Neospora-like organisms were not detected by either periodic acid-schiff (PAS) reaction or immunohistochemical technique. Taken together all these data, this study indicate that N caninum infection was widespread in breeding farms of Korean native cattle, but correlation between malformation and N caninum infection was not recognized.

• Journal of Veterinary Clinics
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• v.33 no.1
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• pp.70-73
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• 2016

Necrotizing meningoencephalitis (NME) is a unique idiopathic nonsuppurative inflammatory disease of central nervous system in small-sized breed dogs. A 9-year-old intact male Shih-Tzu dog with anorexia, vomiting, salivation and intermittent seizures was submitted to the Jeju National University for diagnosis. Grossly, there were no obvious lesions in the brain, except dilatation of most blood vessels in meninges. Histopathologically, brain revealed severe multifocal nonsuppurative inflammation in perivascular area of meninges and cerebral cortex. Some areas of cerebral parenchyma were replaced with lots of macrophages contained periodic acid-Schiff positive materials. Many new-formed blood vessels were observed around the necrotic regions using Gomori reticulum stain. Immunohistochemistry and reverse transcription-polymerase chain reaction were negative for toxoplasmosis and canine distemper virus. Based on the gross, histopathologic features and antigen detection methods, this case was diagnosed as NME. Here we reported the NME in relatively uncommon breed, Shih-Tzu dog, than other small breed dogs.

• Korean Journal of Veterinary Service
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• v.35 no.2
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• pp.147-151
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• 2012

We report large tumors of the left thoracic cavity, which arose from the ribs, were diagnosed as chondrosarcoma in a 4-year-old male mixed dog. The dog was presented with swelling in the left side of the chest wall and lameness. The masses were found to be circumscribed, whitish-grey, large and firm. The cut surface revealed whitish-grey lobules of varying size with cartilaginous consistency. Those were subsequently metastasized to the lung and mandibular lymph node. Histologically, the tumors were made up of clusters of chondroid cells having pleomorphic hyperchromatic nuclei with occasional mitosis. A special and immunohistochemical staining was performed to confirm the diagnosis. Chondroid matrix in tumor showed a positive reaction for alcian blue-periodic acid-Schiff staining. Immunoreactivity to S-100 proteins was present in the cytoplasm and/or nucleus of chondrocytes in tumor. The final diagnosis was grade III chondrosarcoma in the rib, considering histological features in grading criteria.