• 한국식품과학회지
• /
• 제41권6호
• /
• pp.609-614
• /
• 2009

참기름은 높은 항산화활성 및 항암작용 등의 우수한 영양학적 가치를 지니는 반면 비싼 가격으로 인하여 가짜 참기름의 유통이 범람함에 따라 이를 판별할 수 있는 분석 방법의 확립이 요구되는 실정이다. 질량분석기를 바탕으로 한 전자코를 이용하여 들기름이 혼합된 참기름을 제조하여 진위 판별을 시도하였다. 각각의 유지를 전자코를 이용하여 분석, 통계처리하였다. 혼합 참기름의 휘발성 향기성분으로부터 생성되는 ion fragment 중 40-160 amu에서 각 시료 간에 차별성이 높은 fragment(m/z)를 선택하여 해당 intensity값을 판별 분석한 결과, 참기름 및 들기름은 뚜렷하게 구분되었다. 미량의 들기름이 혼합된 참기름은 첨가된 들기름의 농도에 비례하여 제1판별함수값(DF1)과 높은 상관관계를 나타내었다. 참기름의 볶음온도 및 시간 등의 제조조건 및 재배환경, 재배 산지, 제조회사 등의 요인에 의하여 순수한 참기름의 향기패턴이 달라지는 것을 인도산과 국산 참기름을 이용하여 분석하였다. 향후 참기름의 위조 여부를 검증하는 방법에 하나로 활용될 수 있을 것이다.

• Journal of Nutrition and Health
• /
• 제12권2호
• /
• pp.99-105
• /
• 1979

This study was carried out using rats weighing $40{\sim}50\;g$ which were devided into seven groups with various diet compositions emphasizing fat levels of perilla seed oil for the period of 41/2 weeks. The levels of fat in the diet were 5%, 10%, 15% and animals were fed ad libitum. The results are as follows : 1) Yellow pjgmentation of both neck and tail was clearly observed in groups fed 10% and 15% level perilla seed oil without vitamin I supplementation (IV and VII). 2) The growth rate in groups fed 15% level perilla seed oil was reduced as compared to that in groups fed 5% or 10% level perilla seed oil. 3) The mean hematocrit values of 15% level perilla seed oil groups tended to be lower than those of control group, tut the differences were not significant. 4) The serum vitamin I concentration showed different value in various groups, the values of control group were significantly higher than those of perilla seed oil groups-15% level with or without vitamin E supplementation (VI and VII) and 10% level without vitamin E supplementation (IV). According to the results, 10% level-perilla seed oil in the diet can be considered safe if vitamin E is not omitted from the vitamin mixture ana the group fed 15% fat with P/S ratio of 1 appeared to be safe during the experimental period. Finally the long-term studios have to be persued in many aspects by using perilla seed oil in the experimental diet. Because rats are known t4 be quite resistant to the experimental diets, comparative studies using various animal species have to he conducted.

• 동아시아식생활학회지
• /
• 제4권3호
• /
• pp.97-102
• /
• 1994

The oxidative stability of perilla oil were examined by measuring peroxide value. The induction period of perilla oil for each storage temperature was measured by POV and indicated that it was 80 days for 45$^{\circ}C$, 22.5 days for 65$^{\circ}C$, 9.5 days for 85$^{\circ}C$ and 5 days for 105$^{\circ}C$ respectively. Also, the induction period of the perilla oil with different concentration of ginger powder at 85$^{\circ}C$was studied and has been found that 9.4 days for 6% ginger powder, 11.9 days for 4% and 11days for 2% ginger power. The relative antioxidant effectiveness of ginger power was 99% for 6% ginger power, 125% for 4% ginger power, 122% for 2% ginger power. The induction period of perilla oil with gingerol at 85$^{\circ}C$ was 13.5days for 2% crude gingerol, 11.7days for 0.2% crude gingerol and 12.0 days for 0.02% BHT. The elativi antroxidant effectiveness of perilla oil gingerol at 85$^{\circ}C$was 142% for 2% crude gingerol, 123% for 0.2% crude gingerol, 126% for 0.02% BHT.

• 한국식품조리과학회지
• /
• 제15권2호
• /
• pp.114-120
• /
• 1999

The objective of this study was to determine antioxidant effect of ether and ethylacetate fractions of 70% ethanol extract of some food (acid treated or not) on perilla oil. Each fraction of food extract was added to perilla oil and stored for 0,3,6.9,11 days at 60$^{\circ}C$. Then, the peroxide value (POV) of perilla oil samples were analyzed. Perilla oil contained ${\gamma}$-tocopherol 0.6800 $\mu\textrm{g}$/mg, ${\alpha}$-tocopherol 0.3189 $\mu\textrm{g}$/mg. $\delta$-tocopherol 0.0463 $\mu\textrm{g}$/mg, but it was easily oxidized due to high linolenic acid content. To increase yield of ether and ethylacetate fractions from each food extract, the 70% ethanol extract was treated with 0.2% H$_2$SO$_4$ and fractionized by ether and ethylacetate. Among ether and ethylacetate fractions of 70% ethanol extracts of some food, the yield of ethylacetate fraction of acid treated Pueraria thunbergiana extract was 5 times more than that of ethylacetate fraction untreated with acid. Perilla oil which added 100 ppm ethylacetate fraction of acid treated Pueraria thunbergianan extract showed low POV (44.8 meq/kg) compared to POV (80.0 meq/kg) of control.

• BMB Reports
• /
• 제28권5호
• /
• pp.420-426
• /
• 1995

This study was conducted to compare the effects of n-6 linoleic acid and n-3 linolenic acid on lipid peroxidation and the activities of enzymes defending against oxidation, which are involved in the tumor promotion, and histolOgical changes of hepatocarcinogen treated rat liver. In this study, weanling male Sprague-Dawley rats were fed one of three diets, containing 15% (w/w) of beef fat (BF), com oil (CO) or perilla oil (PO), for 11 weeks. During the 3rd week, experimental groups were injected with 2-AAF (50 mg/kg of BW) intraperitoneally 3 times. Findings show that the com oil diet group has greater liver MDA content than the beef fat and perilla oil diet groups. Also, it is observed that the perilla oil diet group has lower MDA content than beef fat and com oil diet groups, even though perilla oil is more desaturated than beef fat and com oil. In terms of activity, mixed-function oxidase activity is not Significantly affected by the different dietary fats and 2-AAF treatment. GSH-peroxidase, GSH-reductase and GSH-Stransferase activities are significantly higher in the CO+AAF group than those of the other groups. GST and GSH-Px are activated by 2-AAF treatment in the com oil diet group only. The hepatocytes of the BF+AAF group were the most severely degenerated, the second was the CO+AAF group and the least was the PO+AAF group. It was also found that dietary com oil increased lipid peroxidation and activated defense enzymes against oxidation in liver, but dietary perilla oil did not, or supressed defense enzymes. Therefore it is concluded that dietary n-3 linolenic acid in perilla oil inhibits lipid peroxidation and carcinoenesis in rat liver following 2-AAF treatment.

• 보존과학연구
• /
• 통권21호
• /
• pp.273-291
• /
• 2000

The perilla oil is painted to the wooden cultural properties of protection of wood and pigment. But that is happened to discolor and gather mold because of the long drying time. So we were put to the test for the improvement of this matter. The result is follows;1. The perilla oil, do not parched domestic Perilla japonica, add to the Japanese acid clay, later passing through the filter paper that the pore size is less than $7\mum$ 2. If the perilla oil add to the antiseptic of Thiazole origin, the mold is suppressed.3. In the painting of perilla oil, the existing Dan-chung paints one time (Luster generation in the more than two times) and the non-existing Dan-Chung paints two times.

• 한국식품영양과학회지
• /
• 제38권3호
• /
• pp.280-286
• /
• 2009

본 연구에서는 참기름, 흑참기름, 들기름 및 올리브유 추출물의 생리활성을 비교, 분석하고자 하였다. 참기름, 흑참 기름, 들기름 및 올리브유 추출수율은 각각 0.94%, 0.70%, 0.48% 및 0.30%로 참기름의 경우, 추출수율이 가장 높았으며, 올리브유의 경우 가장 낮았다. 참기름, 흑참기름, 들기름 및 올리브유 추출물의 총 플라보노이드 함량은 각각 2.7%, 1.9%, 3.0% 및 1.4%이었고, 총 페놀 함량은 각각 6.5%, 4.5%, 4.1% 및 10.1%이었다. DPPH radical 소거능은 참기름> 올리브유> 들기름> 흑참기름 추출물의 순으로, 참기름 추출물이 가장 강한 효과를 보였다. 참기름, 흑참기름, 들기름 및 올리브유의 SOD 유사활성은 1,000 ppm일 때 0.1%, 36.2%, 34.5% 및 31.0%, 5,000 ppm일 때 0.2%, 67.2%, 90.2% 및 46.7%이었다. 아질산염 소거능은 참기름> 흑참기름> 들기름> 올리브유 추출물의 순으로, 참기름 추출물이 가장 강했다. 올리브유 추출물의 경우 B. cereus, M. luteus, E. coli, S. Enteritidis 모두에서 강한 항균효과를 보인 반면, 흑참기름 추출물의 경우는 M. luteus와 E. coli에서만 약한 항균효과를 보였다. 한편 참기름 및 들기름 추출물에서는 항균활성이 나타나지 않았다.

• Journal of Nutrition and Health
• /
• 제22권1호
• /
• pp.42-53
• /
• 1989

The research was designed to study the effect of different fat sources and levels on Body lipid metabolizm and immune responses in Sprague-Dawely strain male rats. These effect of different fat sources compared with sesame Oil, Perilla oil and Beet tallow. Fat sources were divided into 3 groups respectively 7%, 15%, 30% fat level on diet weight basis. The experimental period was 54days. 1) The body weight gain was significantly low in NF group. In Sesame oil group and perilla oil group, low fat level groups were higher than medium, high fat level groups. But in Beef tallow group, high fat level groups were higher than low and Medium groups. 2) The weight of liver, kidney and epididymal fat ped tend to increse with increasing body weight. 3) The contents of triglyceride and total lipid in serum were significantly different with dietary fat sources and Perilla oil group was the lowest. 4) The contents of triglyceride and total lipid in liver were significantly different with dietary fat levels and high fat level group was higher than low fat level group. 5) Perilla oil group, compared with Beef tallow group, showed the higher excretion of cholesterol through feces and the higher deposit of cholesterol in liver. Therefore serum cholesterol level of Perilla oil group was lower than that of Beef tallow group. But eventhough Sesame oil is vegetable oil, Sesame oil did not showed an effect like Perllia oil on serum cholesterol level. 6) Weight of thymus decreased with fat levels particularly in vegetale oil. And it had on effect on mitogen response, mitogen response decreased with fat level in vegetable oil. But in Beef tallow, there was no difference in fat level.

• Environmental Analysis Health and Toxicology
• /
• 제3권1_2호
• /
• pp.9-19
• /
• 1988

The effect of rancid perilla oil on the immune response in mice was studied. ICR male mice were divided into 5 groups and were fed on the experimental diets for 4 weeks. Mice were sensitized and challenged with sheep red blood cell. Immune responses were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette forming cell and macrophage activity. Biochemical items were measured by serum protein and serum albumin. The weight of spleen, thymus and liver were measured. The rancid perilla oil diets decreased humoral and cellular immune responses, the number of peripheral circulating white blood cells and total protein and serum albumin. These results showed that the high rancid perilla oil diet decreased more humoral and cellular immune response, the number of peripheral circulating white blood cells, and total protein and serum albumin than the low rancid perilla oil diet did.

• Environmental Analysis Health and Toxicology
• /
• 제3권3_4호
• /
• pp.17-28
• /
• 1988

The effects of perilla oil diet on the immune response in mice have been studied. ICR male mice were divided into 4 groups and were fed on the experimental diet for 4 weeks. Mice were sensitized and challenged with sheep red blood cell (S-RBC). Immune response were evaluated by antibody production, Arthus reaction, delayed type hypersensitivity (DTH), Rosette forming cell (RFC) and macrophage activity. The weight of body, liver, thymus and spleen were measured. The body weight was increased but thymus weight was not altered by them. The perilla oil diet decreased the weight of liver and spleen in mice. It reduced antibody production, Arthus reaction, DTH and RFC, macrophage activity. These results showed that the high perilla oil diet decresed more humoral and celluar immune response than the low perilla oil diet. It decreased the phagocytic activity on the reticuloendothelial system in mice.