• Genomics & Informatics
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• v.17 no.3
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• pp.28.1-28.9
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• 2019

Bar-code (tag) microarrays of yeast gene-deletion collections facilitate the systematic identification of genes required for growth in any condition of interest. Anti-sense strands of amplified bar-codes hybridize with ~10,000 (5,000 each for up-and down-tags) different kinds of sense-strand probes on an array. In this study, we optimized the hybridization processes of an array for fission yeast. Compared to the first version of the array (11 ㎛, 100K) consisting of three sectors with probe pairs (perfect match and mismatch), the second version (11 ㎛, 48K) could represent ~10,000 up-/ down-tags in quadruplicate along with 1,508 negative controls in quadruplicate and a single set of 1,000 unique negative controls at random dispersed positions without mismatch pairs. For PCR, the optimal annealing temperature (maximizing yield and minimizing extra bands) was 58℃ for both tags. Intriguingly, up-tags required 3× higher amounts of blocking oligonucleotides than down-tags. A 1:1 mix ratio between up- and down-tags was satisfactory. A lower temperature (25℃) was optimal for cultivation instead of a normal temperature (30℃) because of extra temperature-sensitive mutants in a subset of the deletion library. Activation of frozen pooled cells for >1 day showed better resolution of intensity than no activation. A tag intensity analysis showed that tag(s) of 4,316 of the 4,526 strains tested were represented at least once; 3,706 strains were represented by both tags, 4,072 strains by up-tags only, and 3,950 strains by down-tags only. The results indicate that this microarray will be a powerful analytical platform for elucidating currently unknown gene functions.

• Journal of the Earthquake Engineering Society of Korea
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• v.23 no.2
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• pp.101-108
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• 2019

The seismic safety of nuclear power plants has always been emphasized by the effects of accidents. In general, the seismic safety evaluation of nuclear power plants carries out a seismic probabilistic safety assessment. The current probabilistic safety assessment assumes that damage to the structure, system, and components (SSCs) occurs independently to each other or perfect dependently to each other. In case of earthquake events, the failure event occurs with the correlation due to the correlation between the seismic response of the SSCs and the seismic performance of the SSCs. In this study, the EEMS (External Event Mensuration System) code is developed which can perform the seismic probabilistic safety assessment considering correlation. The developed code is verified by comparing with the multiplier n, which is for calculating the joint probability of failure, which is proposed by Mankamo. It is analyzed the changes in seismic fragility curves and seismic risks with correlation. As a result, it was confirmed that the seismic fragility curves and seismic risk change according to the failure correlation coefficient. This means that it is important to select an appropriate failure correlation coefficient in order to perform a seismic probabilistic safety assessment. And also, it was confirmed that carrying out the seismic probabilistic safety assessment in consideration of the seismic correlation provides more realistic results, rather than providing conservative or non-conservative results comparing with that damage to the SSCs occurs independently.

• Journal of the Institute of Electronics and Information Engineers
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• v.53 no.12
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• pp.67-75
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• 2016

DNA computing technology makes the interests on DNA storage and DNA watermarking / steganography that use the DNA information as a newly medium. DNA watermarking that embeds the external watermark into DNA information without the biological mutation needs the reversibility for the perfect recovery of host DNA, the continuous embedding and detecting processing, and the mutation analysis by the watermark. In this paper, we propose a reversible DNA watermarking based on circular histogram shifting of DNA code values with the prevention of false start codon, the preservation of DNA sequence length, and the high watermark capacity, and the blind detection. Our method has the following features. The first is to encode nucleotide bases of 4-character variable to integer code values by code order. It makes the signal processing of DNA sequence easy. The second is to embed the multiple bits of watermark into -order coded value by using circular histogram shifting. The third is to check the possibility of false start codon in the inter or intra code values. Experimental results verified the our method has higher watermark capacity 0.11~0.50 bpn than conventional methods and also the false start codon has not happened in our method.

• International Journal of Air-Conditioning and Refrigeration
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• v.10 no.3
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• pp.117-128
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• 2002

This paper describes the smoke filling process of a fire field model based on a self-deve-loped SMEP (Smoke Movement Estimating Program) code to the simulation of fire induced flows in the two types of atrium space containing a ceiling heat flux. The SMEP using PISO algorithm solves conservation equations for mass, momentum, energy and species, together with those for the modified k-$\varepsilon$ turbulence model with buoyancy production term. Also it solves the radiation equation using the discrete ordinates method. Compressibility is assumed and the perfect gas law is used. Comparison of the calculated upper-layer average tempera-ture and smoke layer clear height with the zone models has shown reasonable agreement. The zone models used are the CFAST and the NBTC one-room. For atrium fires with ceiling glass the ceiling heat flux by solar heat causes a high smoke temperature near the ceiling. However, it has no effect on the smoke movement such as the smoke layer clear heights that are important in fire safety. In conclusion, the smoke layer clear heights that are important in evacuation activity except the early of a fire were not as sensitive as the smoke layer tem-perature to the nature of ceiling heat flux condition. Thus, a fire sensor in atrium with ceiling glass has to consider these phenomena.

• Computers and Concrete
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• v.21 no.3
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• pp.299-310
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• 2018

When reinforced concrete structures are subjected to strong seismic forces, their beam-column connections are very susceptible to be damaged during the earthquake event. Consequently, structural designers try to fit an important quantity of steel reinforcement inside the connection, complicating its construction without a clear justification for this. The aim of this work is to evaluate -and demonstrate- numerically how the quantity and the array of the internal steel reinforcement influences on the nonlinear response of the RC beam-column connection. For this, two specimens (extracted from an experimental test of 12 RC beam-column connections reported in literature) were modeled in the Finite Element code FEAP considering different stirrup's arrays. The nonlinear response of the RC beam-column connection is evaluated taking into account the nonlinear thermodynamic behavior of each component: a damage model is used for concrete; a classical plasticity model is adopted for steel reinforcement; the steel-concrete bonding is considered perfect without degradation. At the end, the experimental responses obtained in the tests are compared to the numerical results, as well as the distribution of shear stresses and damage inside the concrete core of the beam-column connection, which are analyzed for a low and high state of confinement.

• Journal of electromagnetic engineering and science
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• v.10 no.3
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• pp.104-116
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• 2010

With several advantages such as flexible downlink-to-uplink(DL-to-UL) ratio and flexible spectrum usage, Time Division Duplexing(TDD) is emerging as an alternate to Frequency Division Duplexing(FDD), especially in wireless broadband systems. We already have at least four different TDD systems in the industry: Time Division-Synchronous Code Division Multiple Access(TD-SCDMA), IEEE 802.16e-TDD, IEEE 802.16m-TDD, and Time Division-Long Term Evolution(TD-LTE). A disadvantage of TDD is that tight coordination such as time synchronization between adjacent operators is required to prevent interference between the adjacent TDD systems. In this paper, we investigate coexistence scenarios among the above four well-known TDD systems and calculate spectral efficiency(SE) loss in each scenario. Our findings are that SE loss can be significant if TDD ratios of the adjacent operators are considerably different. However, as long as the TDD ratios of the adjacent operators are similar, configurations in the systems permit perfect time synchronization between the two heterogeneous TDD systems, and the resulting SE loss is zero or reasonably low. We believe that the above findings and the configurations of the TDD systems recommended tominimize SE loss will be helpful for operators who deploy TDD systems in system parameter determination and cross-operator coordination.

• International Journal of Aeronautical and Space Sciences
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• v.1 no.1
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• pp.21-28
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• 2000

The flowfield of hypersonic shock-shock interaction has been simulated using a two-dimensional Navier-Stokes code based on AUSMPW+ scheme. AUSMPW+ scheme is a new hybrid flux splitting scheme, which is improved by introducing pressure-based weight functions to eliminate the typical drawbacks of AUSM-type schemes, such as non-monotone pressure solutions. To study the real gas effects, three different gas models are taken into account in the present paper: perfect gas, equilibrium flow and non equilibrium flow. It has been investigated how each gas model influences on the peak surface loading, such as wall pressure and wall heat transfer, and unsteady structure of flowfield in the region of shock-shock interaction. With the results, the value of peak pressure is not sensitive to the real gas effects nor to the wall catalyticity. However, the value of peak heat transfer rates is affected by the real gas effects and the wall catalyticity. Also, the structure of the flowfield changes drastically in the presence of real gas effects.

• Proceedings of the KSME Conference
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• 2000.04a
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• pp.254-260
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• 2000

The spacer grid is one of the main structural components in fuel assembly, which supports the fuel rods, guides cooling water, and protects the fuel assembly from the external impact load such as earthquakes. The nonlinear dynamic impact analysis is conducted by using the finite element code ABAQUS/Explicit. Boundary condition for dynamic analysis is well applied to the test condition. Simulation results also similarly predict the local buckling phenomena. In addition to the buckling parameter, the local buckling cause is examined by both simulation and test method. It is found to correspond well with the test results. Impact tests are also carried out for some specimens of the spacer grid in order to compare the results between the test and the simulation. This test is accomplished by a free fall dummy weight onto the specimen. From this test, only the uppermost and lowermost layers of the multi-cell are buckled, which implies the local buckling at the weakest point of the grid structure.

• Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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• v.19 no.1
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• pp.9-17
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• 2001

GPS is an efficient system in surveying and car navigation. but it is difficult to catch minimum number of satellite when it is hindered by obstacle such as city area. GLONASS system doesn't have perfect constellation yet, but it has many similarities with GPS system in principle of point positioning and signal system. so, it is expected to be much efficient when it is used with GPS system. For the purpose of this, the coordinates of GPS and GLONASS system, the quality of time and frequency was investigated, and the algorithm of point positioning was made. also, the efficiency of GPS/GLONASS combination was presented by analysing the precision of 3D point positioning using C/A code and Yuma satellite orbit information.

• Journal of the Korean Society of Clothing and Textiles
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• v.33 no.1
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• pp.127-138
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• 2009

This study is about the analysis on symbolic meaning and kunstwollen of Issey Miyake's art works which is influenced by various external factors such as social and cultural background, nationality and philosophy. Especially, this research focused on 'Pleats Please Issey Miyake' and 'A Piece of Cloth' which are the core of his works. The most important concept of Issey Miyake's works is the relationship of human body and a piece of cloth. Miyake's clothes has provided the time and the space where inspires the vitality into wearer. He accomplishes the most perfect beauty through incomplete beauty of cloth. Miyake wants to express the emotion of the human being through his clothes and tries to make special code between creator, wearer and viewer. Fashion is accomplished in connection with human body, not exists just as 'clothes'. Fashion has valued as an art which expresses the ideology and the sentiment of human being most directly. Issey Miyake who has tried to express human life and emotion has promoted fashion as an important part of art. Miyake is accepting and delivering the history and culture, expressing and communicating empathy, and combining the different fields harmoniously.