• 한국환경과학회지
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• 제22권7호
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• pp.855-862
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• 2013

Although antioxidant activities of Korean traditional fermented foods were reported by many researchers, study on antioxidant activity of microorganism originated from Korean traditional fermented foods was little. Therefore, we improved condition for antioxidant production by a bacterium isolated from home-made Chungkookjang. We selected a bacterial strain, which showed the highest antioxidative activity, from Chungkookjang and then named GJ. The selected GJ strain was identified as Bacillus methylotrophicus by alignment data of 16S rRNA gene nucleotide sequences. Improved medium compositions for DPPH radical scavenging activity were 0.25% sucrose, 1% peptone, 0.01% $MgSO_4{\cdot}7H_2O$ and initial pH 6.5, respectively. Optimal culture conditions were $30^{\circ}C$, 200 rpm and 4% inoculum volume, respectively. In improved conditions, DPPH radical scavenging activity of GJ reached to 91% in a short time. The strain GJ also possessed ACE inhibition and other antioxidative activities; ACE inhibition activity (49.4%), ABTS radical scavenging activity (99.8%), metal chelating activity (67.9%), SOD-like activity (36.5%) and reducing power ($A_{700}$ = 5.982) were observed, respectively. Therefore, our results suggest that B. methylotrophicus GJ strain may be potential candidate for functional foods, cosmetic products for anti-aging and medicine for diseases caused by oxidative stress.

• 한국식품영양과학회지
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• 제24권3호
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• pp.434-439
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• 1995

단세포 단백질 자원으로 이용 가능한 균주로 알려진 Candida utilis ATCC 42416 균체를 생산하기 위하여 무를 파쇄하여 얻은 무즙을 배지로 균체배양 실험을 하였다. 무즙의 수용성 고형물과 총 당량은 각각 5.0~8.8 Brix와 3.5~6.5%였다. C. utilis ATCC 42416 균주를 무즙에 접종하여 $30^{\circ}C$에서 200rpm으로 진탕 배양하였을 때 24시간 이내에 증식이 끝났으며, 단위 소모당에 대한 최대 균체 생산량은 당농도 1% 회석 무즙에서 L당($5\;\times\;DCRI$) 21.5g의 건조균체를 생산하였다. 5배 회석무즙에 약간의 영양소 glucose(2.0%), yeast extract(0.2%), peptone(0.2%), ammonium sulfate(0.2%) 및 $KH_2PO_4(0.2%)$ 등을 각각 보강하여도 증식 속도, 균체생산 및 세포 단백질 함량 등에 차이가 거의 없어서 무즙자체가 C. utilis ATCC 42416 균체 생산에 좋은 기질이었음을 알 수 있다.

• 한국식품영양과학회지
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• 제25권5호
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• pp.878-884
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• 1996

BailIus sp. IJ-3 균주는 균체외 효소로 대두단백 응고효소를 생산하고, 이 박테리아의 최적 효소생산에 대한 배지조성 및 배양조건을 검토하였다. 최대의 대두단백 응고효소를 생산하는 배지조성은 soymilk 20%(w/v), glucose 2.0%, yeast extract 5.0%, peptone 4.0% 및 potassium Phosphate, monobasic 1.0%였다. 초기 pH는 6.0이었고, $35^{\circ}C$ 배양에서 최대 효소활성을 보였다. 조정된 배지 조성에서 72시간 배양에 최대 효소활성을 나타내었으며, 기존배지 활성의 약 5.3배의 효소활성의 증가를 보였다. BaCilIus sp. IJ-3 균주효소는 7S globu1in에 대해 특이성을 갖고 있는 것으로 사료된다.

• Korean Chemical Engineering Research
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• 제53권4호
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• pp.524-529
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• 2015

본 논문에서는 저온활성 protease의 생산을 최적화하기 위하여 극지 미생물인 Enterobacteriaceae sp. PAMC 25617의 반응표면분석법을 이용한 배지의 최적화를 수행하였다. One-factor-at-a-time 방법을 이용하여 yeast extract, TritonX-100이 protease의 생산에 영향을 미치는 주요인자인 것을 확인하였다. 물리적인 환경 요인으로 pH를 추가하여 반응표면분석 방법을 이용한 최대 protease 생산 농도를 갖는 각 인자들의 농도를 확인한 결과 5 g/L peptone, 3 g/L malt extract, 10 g/L $C_6H_{12}O_6$, 6.690 g/L yeast extract, 0.018 g/L TritonX-100의 농도에 pH 6.777의 조건에서 미생물을 배양하였을 경우, 최대 10.049 U/L의 protease가 생산될 수 있는 것으로 예측되었다. 실제 배양 결과 8.03 U/L의 protease가 얻어졌으며, 최적화 이전의 생산농도와 비교하여 150% 이상의 증가를 이루었다. 결과적으로 배지최적화를 통한 protease 생산량의 증가에 반응표면분석법의 적용이 유용하다는 것을 확인할 수 있는다. 이러한 결과로부터, 배지 최적화를 이용한 극지 미생물 유래 cold-adapted protease 생산량의 증가가 여러 산업 분야에서 유용하게 이용될 수 있을 것으로 생각된다.

• 한국버섯학회지
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• 제3권1호
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• pp.24-30
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• 2005

뽕나무버섯균의 배양적 특징 및 부후특성을 구명하고자 최적 (액체, 한천)배지 선발, 최적 온도 선정, 최적 pH 선정, 최적 톱밥배지를 선발하고, 세포벽 부후 특성을 관찰하였다. 그 결과 최적온도는 $20{\sim}27^{\circ}C$, 최적 pH는 고체 배지에서는 pH 5.0~6.5의 약산성에서 균사생장이 양호하였다. 최적 고체배지로는 ME와 PDA로 선발되었고, 최적 액체배지로는 glucose peptone이 선발되었다. 첨가제로는 탄소원인 lactose, mannitol, 질소원인 glutamic acid가 효과적인 것으로 나타났다. 뽕나무버섯균의 최적 톱밥배지 선발에 있어서 잣나무, 상수리나무, 졸참나무, 소나무에서 균사생육이 양호한 것으로 나타났으며, 삼나무에서 생장이 저조하였다. 뽕나무버섯균에 의해 부후된 침엽수재와 활엽수재 모두 전형적인 백색부후의 부후 형태를 나타내었다. 그러나 삼나무와 아카시나무의 부후는 상대적으로 미약하였다. 이상의 결과는 소나무류(Pinus sp.)의 톱밥이 뽕나무 버섯균의 재배기질로 적합함을 보여주며 일반적으로 흔히 구할 수 있는 저가의 제재부산물인 경송류의 톱밥을 재배 기질로의 활용가능성을 제시한다고 할 수 있다.

• 한국버섯학회지
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• 제11권3호
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• pp.124-130
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• 2013

The effects of environmental and nutritional requirement such as temperature, pH, different media, carbon, nitrogen, and carbon-to-nitrogen ratio on the mycelial growth of Cordyceps militaris strain 'Yedang 3' were studied. The optimum temperature and pH for the growth of mycelium were $20-25^{\circ}C$ and pH 6-7, respectively. Out of ten media tested, mushroom complete media (MCM) was the best medium for fast mycelial growth, and Sabouraud's dextrose agar yeast extract (SDAY), malt extract yeast extract agar (YMA) also were favored. The color and shape of colonies varied in different media. The best carbon sources for mycelial growth were fructose, mannitol, and sucrose, whereas the best nitrogen sources were tryptone and peptone. However, mycelia grew slowly in inorganic nitrogen compounds such as $NH_4Cl$, $(NH_4)_2SO_4$, $NH_4NO_3$, and $NaNO_3$. The optimum C:N ratio observed on the culture media was 30-40 range. These results provided basic information on cultural characteristics of vegetative growth and might be useful for spawn production in Cordyceps militaris.

• 고려인삼학회:학술대회논문집
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• 고려인삼학회 1978년도 학술대회지
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• pp.67-74
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• 1978

The phenomenon of 'soil sickness' is one of the most important limiting factors for ginseng(Panax ginseng) production in Korea. The principal cause is known to be due to the root rots caused by Cylindrocarpon destructans and Fusarium solani. Attempts were made to control the root rots with non-polluting cultural methods or soil amendments. Among the nine soil amendments tested, crab shell, cow bone and pig feces were selected for further testing. Each of the three amendments increased the populations or various actinomycetes in the range of 10-25 times over that of non-amended soil, whereas the population of C. destructans was reduced to about $50-70\%$ as compared with the control. Five isolates of Streptomyces with clear zones on chitin-agar medium were selected and then tested for their antagonistic effects on C. destructans. When anyone of the five isolates of Streptomyces and C. destructans was grown together in a modified peptone broth, growth of the latter was highly inhibited. When three levels of crab shell, cow bone, or pig feces were used to amend potted soil infested with C. destruetans, the root rot ratings of ginseng seedlings were reduced to less than one half in all the treatments as compared to the control. In another similar experiment, crab shell and cow bone amendments resulted in almost complete control of the seedling root rots in soil infested with C. destructans or F. solani. In conclusion, biological control with soil amendments of ginseng root rots caused by C. destructans and F. solani was successful. Further basic studies should be pursued using soil amendments for better control. In addition, field experiments are needed to complement the soil amendment control measures in an integrated pest control program.

• 한국자원식물학회:학술대회논문집
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• 한국자원식물학회 2003년도 춘계 학술발표대회
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• pp.138-139
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• 2003

To investigated the effect of liquid culture conditions and nutrient sources on the formation with bioactive substance of Paeilomyces japonica and Cordyceps militaris cultivated in the country, the result are as follow; The growth temperature of two mycelia is 25$^{\circ}C$ and the proper temperature for cordycepin growth is around 20$^{\circ}C$. The formation amount of bioactive substance by nutrient sources reached its peak with using 2% glucose and 1% galactose in case of carbon sources and 0.4% inorganic compound in case of nitrogen sources. Also, the ratio of C/N was optimal with 3% glucose 1% peptone. For a natural medium, most grains were sufficient but the soybean oil was superb. The formation amount of protein-binding polysaccharide that are used for anticancer substance was in proportion to the growth rate of mycelium, had lots of aeration and showed a trend of increasing when the acidity lower. and the content of structural protein showed a trend of increasing when the acidity lower. However, the content of the structural hexosamin did not get a great the effect of culture conditions and nutrient sources. The constitution of monosaccharide that organizes a protein-binding polysaccharide greatly changed in proportion to carbon sources. When Paecilomyces japonica cultured in a silkworm larvae for 30 days, the content of cordycepin was 204.5 mg/100mL as a dry weight in the fruiting body, 41.8 mg/100mL in mycelium and larva, and the content for each bottle was average 29.5 mg/100mL. In case of Cordyceps militaris for 45days, the fruiting body was 563.5 mg/100mL, the larva and the mycelium was 86.1 mg/100mL, and the content for each bottle was average 65.0 mg/100mL.

• 한국균학회지
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• 제12권4호
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• pp.129-132
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• 1984

한국산 담자균류로부터 새로운 항종양 성분을 개발하기 위하여 표고의 일개 균주인 Lentinus edodes DMC7을 인공배지에서 진탕배양하여 그 균사 배양물을 얻었다. 그중 배양 여액으로부터 수용성 고분자 분획을 분리하여 LF-3라고 명명하였다. LF-3는 ICR 마우스에 이식된 Sarcoma 180세포에 대하여 항암작용을 나타내었으며 그 저지율은 53. 1%였다.

• 미생물학회지
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• 제6권4호
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• pp.113-121
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• 1968

This study has been aimed to determine the ecological factors with relationship to the Jongkok production in view of fermentation technology by means of some strains, Asp. kawachii, which is now preserved by the author and the following factors are included during the study; inorganic salts, nitrogen, sugar, water contents and temperature. The results, are as follows: (a) Sugar among other above-mentioned factors is increasingly affecting the number of the short type of conidiophore on culture medium and the conidiophore is increased by direct ratio until glucose concentration of 50%, at which concentration is mostly effective for the short type of conidiophore, while other factors did not affect on it. (b) Until glucose concentration of 50% sugar component of culture medium is favorable for the spore formation of Asp. kawachii by direct ratio. And peptone or asparagine on nitrogen medium, calcium-phosphate among other inorganic salts, wheat bran and rice branare also favorable, but other factors rientioned earlier show no relationship with the spore formation. Sugar, however, also related with the spore color clearness of crimson and light brown, and spore color is mostly clear at the point of glucose concentration until 50%. And asparagine on nitrogen medium, calcium phosphate among other inorganic salts, rice bran did all affect on the color clearness, while other factors did not concern with color clearness. (c) Water, sugar and temperature have related with the acid formation which is promoted, by direct ratio at the point of water-saturated condition and glucose concentration of 50%, while temperature at $25^{\circ}C$favorably affected on the acid formation which is increased by inverse ratio at the temperature$25^{\circ}C$ to $45^{\circ}C$ And pH did not relate with the acid formation. (d) Cylindrical plate method devised by the author is mostly favorable for the preservation and isolation of culture, compared with the traditional slant medium method.