Browse > Article
http://dx.doi.org/10.9713/kcer.2015.53.4.524

Optimization of Medium for Protease Production by Enterobacteriaceae sp. PAMC 25617 by Response Surface Methodology  

Kim, Hyun-do (Department of Biotechnology and Bioengineering, Chonnam National University)
Yun, Chul-Won (Department of Biotechnology and Bioengineering, Chonnam National University)
Choi, Jong-il (Department of Biotechnology and Bioengineering, Chonnam National University)
Han, Se Jong (Division of Life Sciences, Korea Polar Research Institute)
Publication Information
Korean Chemical Engineering Research / v.53, no.4, 2015 , pp. 524-529 More about this Journal
Abstract
This study was conducted to optimize the medium composition for cold-adaptive protease production of Enterobacteriaceae sp. by response surface methodology (RSM). Yeast extract, and TritonX-100 were identified as the significant factors affecting protease from one-factor-at-a-time method. RSM studies for optimizing protease production of Enterobacteriaceae sp. have been carried out for three parameters including yeast extract concentration, TritonX-100 concentration, and culture pH. These significant factors were optimized as 6.690 g/L yeast extract, 0.018 g/L Triton$^{TM}$ X-10, and pH 6.677. The experimentally obtained protease activity was 8.03 U /L, and it became 1.5-fold increase before optimization.
Keywords
Antarctic Microorganism; Protease; Response Surface Methodology;
Citations & Related Records
Times Cited By KSCI : 5  (Citation Analysis)
연도 인용수 순위
1 Georlette, D., Blaise, V., Collins, T., D'Amico, S., Gratia, E., Hoyoux, A., Marx, J., Sonan, G., Feller, G. and Gerday, C., "Some Like it Cold: Biocatalysis at Low Temperatures," FEMS microbial. Rev., 28, 25-42(2004).
2 Yang, J., Li, J., Mai, Z., Tian, X. and Zhang, S., "Purification, Characterization, and Gene Cloning of a Cold-adapted Thermolysin-Like Protease from Halobacillus sp. SCSIO 20089," J. Biosci. Bioeng., 115, 628-632(2013).   DOI
3 Gerday, C., Aittaleb, M., Bentahir, M., Chessa, J. P., Claverie, P., Collins, T., D'Amico, S., Dumont, J., Garsoux, G., Georlette, D., Hoyoux, A., Lonhienne, T., Meuwis, M. A. and Feller G., "Coldadapted Enzymes: from Fundamentals to Biotechnology," Trends. Biotechnol., 18, 103-107(2000).   DOI
4 Dastager, S. G., Dayanand, A., Li, W. J., Kim, C. J., Lee, J. C., Park, D. J., Tian, X. P. and Raziuddin, Q.S., "Proteolytic Activity from An Alkali-thermotolerant Streptomyces gulbargensis sp. nov," Curr. Microbiol., 57, 638-642(2008).   DOI
5 Kirk, O., Borchert, T. V. and Fuglsang, C. C., "Industrial Enzyme Applications," Curr. Opin. Biotechnol., 13, 345-351(2002).   DOI
6 Cherry, J. R. and Fidantsef, A. L., "Directed Evolution of Industrial Enzymes: An Update," Curr. Opin. Biotechnol., 14, 438-443(2003).
7 Daniel R. M., Toogood, H. S. and Bergquist, P. L., "Thermostable Proteases," Biotech. Genet. Eng. Rev., 13, 51-100(1996).   DOI
8 Gupta, R., Beg, Q. and Lorenz, P., "Bacterial Alkaline Proteases: Molecular Approaches and Industrial Applications," Appl. Microbial. Biotechnol., 59, 15-32(2002).   DOI
9 Anisworth, S. J., "Soap & Detergents," Chem. Eng. News., 70, 27-37(1992).
10 Marx, J. C., Collins, T., D'Amico, S., Feller, G. and Gerday, C., "Cold-adapted Enzymes from Marine Antarctic Microorganisms," Marine Biotechnol., 9, 293-304(2007).   DOI
11 Cavicchioli, R., Siddiqui, K. S., Andrews, D. and Sowers, K. R., "Low-temperature Extremophiles and Their Applications," Curr. Opin. Biotechnol., 13, 253-261(2002).   DOI
12 Wang, Q., Hou, Y., Xu, Z., Miao, J. and Li, G., "Optimization of Cold-active Protease Production by the Psychrophilic Bacterium Colwellia sp. NJ341 with Response Surface Methodology," Biores. Technol., 99, 1926-1931(2008).   DOI
13 Irwin, J. A., Alfredsson, G. A., Lanzetti, A. J., Gudmundsson, H. M. and Engel, P. C., "Purification and Characterisation of a Serine Peptidase from the Marine Psychrophile Strain PA-43," FEMS Microbiol. Let., 201, 285-290(2001).   DOI
14 Siddiqui, K. S. and Cavicchioli, R., "Cold-adapted Enzymes," Annual Rev. Biochem., 75, 403-433(2006).   DOI
15 Kackar, R. N., "Off-line Quality Control, Parameter Design, and the Taguchi Method," J. Quality Technol., 17, 176-188(1985).   DOI
16 Ghani, J. A., Choudhury, I. A. and Hassan, H. H. "Application of Taguchi Method in the Optimization of End Milling Parameters," J. Mater. Process. Technol., 145, 84-92(2004).   DOI
17 Tsai, J. T., Liu, T. K. and Chou, J. H. "Hybrid Taguchi-genetic Algorithm for Global Numerical Optimization," Evolutionary Computation, IEEE Transactions on, 8, 365-377(2004).   DOI
18 Puri, S., Beg, Q. K. and Gupta, R., "Optimization of Alkaline Protease Production from Bacillus sp. By Response Surface Methodology," Curr, Microbiol., 44, 286-290(2002).   DOI
19 Dutta, J. R., Dutta, P. K. and Banerjee, R., "Optimization of Culture Parameters for Extracellular Protease Production from a Newly Isolated Pseudomonas sp. Using Response Surface and Artificial Neural Network Models," Proc. Biochem., 39, 2193-2198(2004).   DOI
20 Adinarayana, K. and Ellaiah, P., "Response Surface Optimization of the Critical Medium Components for the Production of Alka-Line Protease by a Newly Isolated Bacillus sp.," J. Pharmacy. Pharmaceutical Sci., 5, 272-278(2002).
21 Hanlon, G. W., Hodges, N. A. and Russel, A. D., "The Influence of Glucose, Ammonium and Magnesium Availability on the Production of Protease and Bacitracin by Bacillus Licheniformis," J. General Microbiol., 128, 845-851(1982).
22 Tabaraki, R. and Rastgoo, S., "Comparison Between Conventional and Ultrasound-assisted Extractions of Natural Antioxidants from Walnut Green Husk," Korean J. Chem. Eng., 31, 676-683(2014)   DOI
23 İlbay, Z., Sahin, S. and Buyukkabasakal, K., "A Novel Approach for Olive Leaf Extraction Through Ultrasound Technology: Response Surface Methodology Versus Artificial Neural Networks," Korean J. Chem. Eng., 31, 1661-1667(2014).   DOI
24 Toon, C. H., Bok, H. S., Choi, D. K. and Row, K. H., "Optimization Condition of Astaxanthin Extract from Shrimp Waste Using Response Surface Methodology," Korean Chem. Eng. Res., 50, 545-550(2012).   DOI   ScienceOn
25 Kin, J. W., "Response Surface Optimization of Fermentation Parameters for Citric Acid Production in Solid Substrate Fermentation," Korean Chem. Eng. Res., 50, 879-884(2012).   DOI   ScienceOn
26 Thys, R., Guzzon, S. O., Cladera-Olivera, F. and Brandelli, A., "Optimization of Protease Production by Microbacterium sp. in Feather Meal Using Response Surface Methodology," Proc. Biochem., 41, 67-73(2006).   DOI
27 Nichols, D., Bowman, J., Sanderson, K., Nichols, C. M., Lewis, T., McMeekin, T. and Nichols, P. D., "Developments with Antarctic Microorganisms: Culture Collections, Bioactivity Screening, Taxonomy, PUFA Production and Cold-adapted Enzymes," Curr. Opin. Biotechnol." 10, 240-246(1999).   DOI
28 De Coninck, J., Bouquelet, S., Dumortier, V., Duyme, F. and Verdier-Denantes, I., "Industrial Media and Fermentation Process for Improved Growth and Protease Production by Tetrahymena thermophila BIII," J. Ind. Microbiol. Biotechnol., 24, 285-290(2000).   DOI
29 Plotnick, M. I., Rubin, H. and Schechter, N. M., "The Effects of Reactive Site Location on the Inhibitory Properties of the Serpin ${\alpha}_{1}$ - Antichymotrypsin," J. Biol. Chem., 277, 29927-29935(2002).   DOI
30 McKeller, R. C. and Cholette, H., "Synthesis of Extracellular Proteinase by Pseudomonas fluorescens Under Conditions of Limiting Carbon, Nitrogen and Phosphate," Appl. Biochem. Microbiol., 47, 1224-1227(1984).
31 Antranikian, G. and Egorova, K., "Extremophiles, a Unique Resource of Biocatalysts for Industrial Biotechnology," Physiology and Biochemistry of Extremophiles. ASM Press. Washington, 361-406(2007).
32 Huston, A. L., "Biotechnological Aspects of Cold-adapted Enzymes," In Psychrophiles: From Biodiversity to Biotechnology. Springer. Berlin. Heidelberg, 347-363(2008).
33 Kim, D. K., Park, H. J., Lee, Y. M., Hong, S. G., Lee, H. K. and Yim, J. H., "Screening for Cold-Active Protease-Producing Bacteria from the Culture Collection of Polar Microorganisms and Characterization of Proteolytic Activities," Korean J. Microbiol., 46, 73-79(2010).
34 Zhu, H. Y., Tian, Y., Hou, Y. H. and Wang, T. H., "Purification and Characterization of the Cold-active Alkaline Protease from Marine Cold-adaptive Penicillium Chrysogenum FS010," Mol. Boil. rep., 36, 2169-2174(2009).   DOI