• 한국균학회지
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• 제31권1호
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• pp.14-21
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• 2003

본 연구는 섬유소분해능이 우수한 L. japonicus의 균사생육에 미치는 중요 인자에 대한 배양학적 특성을 규명하여 인공재배 및 균사체 배양을 이용한 섬유소분해효소의 생산을 높일 수 있는 기초자료를 얻고자 실험을 수행하였다. 그 결과, 균사체 배양의 최적온도는 $30^{\circ}C$이었으며, 최적 pH 는 6.0이었다. 균사생장에 적합한 배지는 glucose peptone 배지, malt yeast extract 배지, yeast malt peptone 배지, potato dextrose 배지, Hennerberg 배지 등으로 조사되었다. 균사생장에 가장 양호한 탄소원은 dextrose 였으며, 최적농도는 1.2%이었다. 또한 질소원으로서 균사생장에 가장 양호한 유기, 무기질소원, 아미노산의 종류 및 최적농도는 각각 yeast extract(1.7%), $(NH_4)_2HPO_4(0.2%)$, glutamine(0.2%) 이었다. 균사생장에 양호한 무기염류는 $Al_2(SO_4)_3{\cdot}14H_2O$인 것으로 조사되었으며 최적농도는 0.1 M 이었다.

• 식물조직배양학회지
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• 제25권3호
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• pp.189-194
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• 1998

우리나라 야생란 중 원예적 개발가치가 인정되나 대량 증식체계가 확립되지 않아 원예종으로 보급이 미흡한 새우난초 및 해오라비난초를 중심으로 종자의 기내발아와 유묘의 생장촉진에 미치는 배지의 영향에 대해 실험한 결과는 다음과 같다. 새우난초의 발아 및 유묘 생장은 파종전 초음파를 30분간 처리한 후 MS배지에 파종하여 얻은 유묘를 H$_3$P$_4$배지에 이식하여 육묘했을 때 가장 적합하였다. 해오라비난초는 종자를 H$_3$P$_2$배지에 파종하여 형성된 구근을 H$_3$P$_1$ 배지에 이식하여 맹아시킨 후 H$_1$P$_2$배지에 옮겨 배양했을 때 우량종구의 기내생산이 가능하였다.

• KSBB Journal
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• 제14권2호
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• pp.167-173
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• 1999

A new synthetic medium was developed for the submerged mycelial cultures of Phellinus linteus. The medium for maximum mycelial growth of Phellinus linteus (3 days incubation, 28$^{\circ}C$, pH 5) consisted of (per 1 L): glucose, 90 g peptone, 10 g soluble starch, 10 g yeast extract, 3 g KH2PO4, 1 g MgSO4.7H2O, 1 g and CaCl2, 0.1 g. The concentrations of glucose, peptone, yeast extract, KH2PO4, MgSO4.7H2O, and CaCl2 were examined in the ranges of 10~90 g/L, 0~10 g/L, 0~15 g/L, 0~2 g/L, 0~1 g/L, and 0~0.5 g/L, respectively. The dry weight of mycelium in 3 days increased to 16.79 mg/mL using the new synthetic medium. The optimum temperature for mycelial growth of Phellinus linteus was 28$^{\circ}C$. The concentrations of KH2OP4, CaCl2, and yeast extract, which gave the maximum mycelial growth of Phellinus linteus, existed in the concentration ranges examined in this study. But, in the cases of other compositions (MgSO4.7H2O, peptone, and glucose), the mycelial growth of Phellinus linteus increased with the concentration in the ranges.

• 한국축산식품학회지
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• 제38권2호
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• pp.240-250
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• 2018

This study was undertaken to find the optimum soy-peptone, glucose, yeast extract, and magnesium sulfate amounts for the maximum growth of Lactobacillus plantarum JNU 2116 and to assess the effects of these medium factors through the use of response surface methodology. A central composite design was used as the experimental design for the allocation of treatment combinations. In the analysis of the experiment, due to a significant lack of fit of the second-order polynomial regression model that was used at first, cubic terms were added to the model, and then two-way interaction terms were deleted from the model since they were found to be all statistically insignificant. A relative comparison among the four factors showed that the growth of L. plantarum JNU 2116 was affected strongly by yeast extract, moderately by glucose and peptone, and slightly by magnesium sulfate. The estimated optimum amounts of the medium factors for the growth of L. plantarum JNU 2116 are as follows: soy-peptone 0.213%, glucose 1.232%, yeast extract 1.97%, and magnesium sulfate 0.08%. These results may contribute to the production of L. plantarum L67 as a starter culture that may have potential application in yogurt and fermented meat products.

• 한국미생물·생명공학회지
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• 제6권4호
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• pp.167-171
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• 1978

전보에 개발된 배지에 국산 포도당을 이용한 발효결과, 구연산 생성은 37∼43g/ι이었다. 제품X의 경우는 제품Y에 비해 구연산 생성이 우수하여 43g/ι가 생성되었다. 국산 포도당을 Ambelite-IR -120으로 처리하고, peptone도 potassium ferrocyan-ide로 처리하여 금속이온을 제거한 뒤에, 10mg/ι의 철 이온을 첨가하였을 때 구연산 생성은 53gm/ι에 달하였다. 또한 원래 Sakaguchi 배지에 비해 3배나 많이 구연산이 생성되었다. 분리 동정된 Asp. niger를 다른 문헌과 비교하였을 때 큰 손색이 없었으며, 앞으로의 생리연구 및 mutation 연구가 필요하겠다.

• Journal of Applied Biological Chemistry
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• 제48권1호
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• pp.11-15
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• 2005

Salinity of soils in greenhouse has been increased by massive application of fertilizers. Nitrogen fertilizer was most popular, and thus nitrate became the majority of soil salinity. Accumulation of nitrate led to deleterious effects on the growth and development of crops and vegetables. Microbial strains able to utilize nitrate and thus remove excess nitrate from farm land soils were isolated from 15 different soils of greenhouses and plastic film houses. Four strains able to grow in medium containing 50 mM $KNO_3$ were isolated, among which only E0461 showed high capacity of nitrate uptake. Nitrate uptake by E0461 was dependent on culture medium and was increased by addition of tryptone and peptone. Although E0461 was able to grow without tryptone and peptone, growth was slow, and no nitrate uptake was observed. Nitrate appeared to facilitate E0461 growth in the presence of tryptone and peptone. Through kinetic analysis, nitrate uptake was measured at various concentrations of nitrate, and half-life was calculated. Nitrate concentration decreased with increasing incubation period, and plot between half-lives and initial concentrations of nitrate fitted to single exponential function. These results suggest one major factor plays an important role in microbial nitrate uptake.

• Journal of Microbiology and Biotechnology
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• 제23권10호
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• pp.1422-1427
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• 2013

Scutellaria baicalensis (SB), a traditional herb with high pharmacological value, contains more than 10% flavone by weight. To improve the biological activity of flavones in SB, we aimed to enhance the bioconversion of baicalin (BG) to baicalein (B) and wogonoside (WG) to wogonin (W) in SB during fermentation using beta-glucuronidase produced from Lactobacillus brevis RO1. After activation, L. brevis RO1 was cultured in milk containing SB root extract with various carbon or nitrogen sources at $37^{\circ}C$ for 72 h. During fermentation, the growth patterns of L. brevis RO1 and changes in the flavone content were assessed using thin-layer chromatography and high-performance liquid chromatography. After 72 h of fermentation, the concentrations of B and W in the control group increased by only 0.15 and 0.12 mM, respectively, whereas they increased by 0.57 and 0.24 mM in the fish peptone group. The production of B and W was enhanced by the addition of 0.4% fish peptone, which not only improved the growth of L. brevis RO1 (p < 0.001) but also enhanced the bioconversion of flavones. In conclusion, the bioconversion of flavones in SB may provide a potential application for the enhancement of the functional components in SB.

• Journal of Microbiology
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• 제35권2호
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• pp.103-108
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• 1997

To improve the detection of methicillin resistant staphylococci, lowered incubation temperature (30.deg.) and inclusion of sodium chloride in media have been empirically recommended. However, in this study, we found that sodium chloride in Peptone-Yeast Extract-K$\_$2/HPO$\_$4/ (PYK) medium decreased methicillin minimum inhibitory concentrations. Divalent cations were shown to restore the expression of staphylococcal methicillin resistance. However, when it was determined by efficiency of plating, sodium chloride increased methicillin resistance expression on agar medium in which higher divalent cations were contained in the agar medium. The decrease of minimum inhibitory concentrations at 30.deg.C by sodium chloride occurred in Brain Heart Infusion but did not occur in other media investigated. Interestingly, both PYK and Brain Heart Infusion media had peptone, which contain cholic acids having detergent activities. Inclusion of sodium chloride in PYK caused a higher rate of autolysis. Penicillin binding protein 2a that has a low affinity to beta-lactam antibiotics, was highly inducible in methicillin resistant Staphylococcus epidermidis strains. In this study, we found that autolysins that are activated by the sodium chloride decreased the minimum inhibitory concentration at 30.deg.C, and peptidoglycan is weakened due to the presence of methicillin. Peptone in the media may aggravate the fragile cells. However, stabilization due to the presence of divalent cations and production of penicilin binding protein 2a increase the survival of staphylococci.

• 한국버섯학회지
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• 제3권2호
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• pp.75-78
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• 2005

노랑느타리버섯 균사생장에 대한 영양원에 대한 연구의 결과는 다음과 같다. 1) 노랑느타리버섯의 균사생장에 적합한 탄소원은 maltose로 균사생장량은 357mg/25ml/15일이다. 2) 노랑느타리버섯의 균사생장에 적합한 질소원은 peptone으로 균사생장량은 374mg/25ml/15일이다. 3) 노랑느타리버섯의 균사생장에 적합한 유기산은 glutamic acid로 균사생장량은 389mg이다. 4) 노랑느타리버섯의 균사생장에 적합한 비타민은 biotin으로 균사생장량은 399mg/15일이다. 5) 노랑느타리버섯의 균사생장에 적합한 C/N율은 40이다.

• KSBB Journal
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• 제17권2호
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• pp.169-175
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• 2002

원유로부터 다당류를 생산하는 유산균을 분리, 선발하여 균의 배양조건을 조사하고 Lactobacillus ssp. SCU-M으로 동정, 명명된 최적배지의 탄소원은 galactose 1.5% 첨가시 553 mg/L의 EPS를 생성하였으며 whey의 이용성 검토를 위한 deproteinized whey와 lactic acidified whey를 탄소원으로 사용하였을 때 각각 639, 550 mg/L를 생성하였다. 질소원으로 yeast extract 1.5%, peptone 0.5%를 첨가한 경우 934 mg/L, 미량성분으로 , 0.15% $K_2$HPO, 0.15% 첨가시 1,076 mg/L의 다당 생산성을 나타내었다. 따라서 이들 결과를 종합하여 galactose 1.50%, yeast extract 1.00%, peptone 0.25%, MgSO$_4$, 7$H_2O$ 0.15%, $K_2$HPO$_4$0.15%, tween 80, 0.10%를 적정 배지로 하여 PH 6.5, 배양온도 37$^{\circ}C$에서 배양시 1,680 mg/L의 EPS를 생성하였다.