• 한국재료학회지
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• 제33권2호
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• pp.47-53
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• 2023

When exposed to different types of bacteria in the oral cavity, denture based resins are prone to bacteria attachment. The purpose of this study was to investigate the physical, biological, and antimicrobial properties of denture base resins coated with Peony extract (200, 400, and 600 ㎍/mL). Specifically, the surface properties (microhardness, contact angle, and color change of the coated specimens), cell cytotoxicity (measured using MTT assay), and antimicrobial activity (against S. mutans (Streptococcus mutans) and C. albicans (Candida albicans) using a growth inhibition assay) were evaluated. The polyphenol content was measured using ultraviolet-visible (UV-vis) spectrometry. The experimental groups (specimens coated with Peony extract) and a control group (specimens coated without Peony extract) were statistically compared using a one-way analysis of variance and Tukey's post-hoc tests. No statistically significant differences in surface properties or cell cytotoxicity were observed, which demonstrated their biocompatibility. Conversely, a statistically significant difference in antimicrobial activity was observed between the experimental and control groups after 48 h. This confirms the antimicrobial activity of the denture base resin coated with Peony extract and demonstrates that it is a promising dental material for preventing stomatitis.

• 한국환경농학회지
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• 제29권1호
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• pp.7-11
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• 2010

This experiment was conducted to investigate the growth inhibition effects caused by continuous cropping soil in peony(Paeonia lactiflora Pallas). The effect of extracts from continuous cropping soil of peony was tested with bio-assay method using callus cells induced from peony filament tissues and seedlings derived from peony zygotic embryos. The cell viability and seedling growth were significantly inhibited by methanol extract in continuous cropping soil. Methanol extract from continuous cropping soil was successively fractionated with solvents such as n-hexane, ethyl acetate, n-butanol and water. The seedling growth was inhibited by ethyl acetate fraction obtained in methanol extract.

• 한국식품저장유통학회지
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• 제4권1호
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• pp.69-75
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• 1997

Peony is a medicinal herb which have utilized widely as chineses medicine. The paeoniflorin is the predominant component In peony root, monoterpene glucoside containing pinane structure. The effective components were extracted with the cold water from the intact peony roots, and effectively extracted with 70% ethanol from the dry powder of peony roots. The changes of paeoniflorin contents were investigated during the drying process of peony roots and processing of peony extract by the heat-treatment. Air-drying was the best condition for the preservation of paeoniflorin content among the drying processes of peony roots. But convective drying at 6$0^{\circ}C$ was recommended for the drying process of peony roots in large scale. The paeoniflorin in peony extracts was not destroyed by the treatment at 6$0^{\circ}C$ and 8$0^{\circ}C$ for 5 hrs, but destroyed 30%, 28% and 40% of paeoniflorin by treatment at 10$0^{\circ}C$ for 5 hrs, 115$^{\circ}C$ and 121$^{\circ}C$ for 10 minutes, respectively. The paeoniflorin was continueously extracted for 4 hrs from the dry pieces of peony roots(0.5$\times$0.5$\times$0.5cm) in boiling water but destroyed gradually after 4 hrs at 10$0^{\circ}C$. Paeoniflorins in 70% ethanol extracts of peony root were not destroyed at all in the process of concentration to dry powder at 60"C on vacuum.cuum.

• 생약학회지
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• 제10권3호
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• pp.119-124
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• 1979

Both wild peony and cultivated peony demonstrated a slight effect and tranquilizer of anti-convulsion on the strychinine and picrotoxine but the wild plant was more effective. The methanol extract of cultivated peony root demonstrated a weak effect of muscle loosening but its mixture with water extractable fraction did not have this effect in contrast to the case wild type whose methanol extract mixture with water extractable fraction still retained a strong anti-convulsional muscle loosening effect. Therefore, it is concluded that wild peony root has more analgesic and muscle loosening effect than the cultivated counterpart has.

• Food Science and Biotechnology
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• 제16권6호
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• pp.1055-1059
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• 2007

Two monoterpene glucosides, paeoniflorin and albiflorin, in peony (Paeonia lactiflora) were purified from 70% ethanol extract of Paeoniae Radix by diethyl ether washing and n-butanol partition, acetone dissolution, and gradient preparative HPLC. After the whole course of purification, yield of paeoniflorin, albiflorin, and the sum of them were 75.0, 38.8, and 68.7%, respectively, together with the corresponding purity of 96.2, 93.8, and 96.0%.

• 대한화장품학회지
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• 제49권3호
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• pp.259-267
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• 2023

본 연구에서는 작약꽃을 가수분해하여 플라보노이드계 화합물인 peonidin 배당체를 비배당체로 전환하여 생리 활성을 증대시키고 신규 화장품 소재로 이용하고자 하였다. 이를 위해 품종별 작약꽃을 추출하여 유효성분 함량이 가장 높은 품종을 선정하였으며, 해당 품종을 이용하여 가수분해를 진행하였다. 이를 통해 고농도의 hydrochloric acid (HCl) 처리가 가수분해에 가장 적합함을 확인하였으며, 효소를 이용한 생물학적 가수분해는 효과가 없음을 확인하였다. 작약꽃 가수분해물에는 붉은색을 나타내는 안토시아닌계 화합물인 peonidin 배당체가 peonidin으로 전환되어 63.3 ppm 함유되어 있음을 분석을 통해 확인하였다. 작약꽃 가수분해물의 생리 활성을 확인해본 결과, 가수분해물이 일반 작약꽃추출물 대비 2,2-diphenyl-1-picrylhydrazyl (DPPH) 라디칼을 소거하는 항산화 활성이 우수하며, nitric oxide (NO) 생성을 저해하는 항염 효과가 증대하였음을 확인할 수 있었다. 또한, ultraviolet A (UVA)에 의한 피부 섬유아세포 손상을 감소시켜 광노화를 억제함을 확인하였다. 따라서 본 연구를 통해 작약꽃 가수분해물이 화장품 산업에서 신규 항노화 소재로 활용이 가능할 것으로 기대된다.

• Archives of Pharmacal Research
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• 제28권7호
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• pp.775-783
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• 2005

The ethanol extract of the peony root (Paeonia Lactiflora Pall, Paeoniaceae) as well as its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. In addition, the protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were examined. The ethanol extracts of the peony root (PREs) and its active constituents, gallic acid and methyl gallate, exhibited a significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and had an inhibitory effect on lipid peroxidation, as measured by the level of malondialdehyde (MDA) formation. The PREs did not have any pro-oxidant effect. They strongly inhibited the hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, as assessed by single cell gel electrophoresis. Furthermore, the oral administration of 50% PRE (50% ethanol extract of peony root), gallic acid and methyl gallate potently inhibited the formation of micronucleated reticulocytes (MNRET) in the mouse peripheral blood induced by a $KBrO_3$ treatment in vivo. Therefore, PREs containing gallic acid and methyl gallate may be a useful antigenotoxic antioxidant by scavenging free radicals, inhibiting lipid peroxidation and protecting against oxidative DNA damage without exhibiting any pro-oxidant effect.

• 한국응용약물학회:학술대회논문집
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• 한국응용약물학회 2003년도 Annual Meeting of KSAP : International Symposium on Pharmaceutical and Biomedical Sciences on Obesity
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• pp.61-61
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• 2003

The ethanol extract of peony root (Paeonia Lactiflora Pall, Paeoniaceae) and its major active components including gallic acid and methyl gallate were evaluated for their protective effects against free radical generation and lipid peroxidation. And protective effects against hydrogen peroxide-induced oxidative DNA damage in a mammalian cell line were performed. The ethanol extract of peony root (PRE), gallic acid and methyl gallate were shown to possess the significant free radical scavenging effect against 1,1-diphenyl-2-picryl hydrazine (DPPH) radical generation and were revealed the inhibitory effect of lipid peroxidation as expressed by malondialdehyde (MDA) formation. They were also found to strongly inhibit hydrogen peroxide-induced DNA damage from NIH/3T3 fibroblasts, assessed by single cell gel electrophoresis. Furthermore, oral administration of 50% PRE (50% ethanol extract), gallic acid and methyl gallate potently inhibited micronucleated reticulocyte (MNRET) formation of mouse peripheral blood induced by KBrO3 treatment in vivo. Therefore, PRE containing gallic acid and methyl gallate may be a useful natural antioxidant by scavenging free radicals, inhibition of lipid peroxidation and protecting oxidative DNA damage.

• Current Research on Agriculture and Life Sciences
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• 제14권
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• pp.15-28
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• 1996

주(主) 재배품종인 의성종과 영천종의 유효성분을 HPLC 및 TLC로 분석한 결과 주(主)성분인 paeoniflorin 함량은 의성종이 영천종보다 높으며 albiflorin과 oxypaeoniflorin의 함량을 두 재배종에서 비교하면 의성종은 albiflorin 함량이 많고 영천종은 oxypaeoniflorin의 함량이 많았다. 생산지에 따른 paeoniflorin의 함량차이는 2.15% 에서 4.08% 의 범위였고 의성과 거창의 것은 비슷하였으며 영천의 것이 가장 낮았다. 18종의 재배종들은 품종에 따라 다양한 paeoniflorin 함량 차이를 보이며 작약성분분석의 HPLC 크로마토그랍을 albiflorin, oxypaeoniflorin 및 paeoniflorin 피크를 기준으로 비교해본 결과 크게 3가지 패턴으로 분류할수 있었다. 이 세가지 유형은 의성종 패턴 9종, 영천종 패턴 4종 풍기표준종패턴 5종 이었다. 수확시기에 따른 작약의 paeoniflorin의 함량변화를 조사한 결과 3월과 9월에 수확한 것이 낮았으며 5월과 11월에 수확한 작약의 paeoniflorin 함량이 높았다.

• 한국약용작물학회지
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• 제11권3호
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• pp.201-206
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• 2003

한국산 재배작약의 품질평가 기준 확립을 위한 연구의 일환으로 국내 재매작약의 생육년수, 품종, 건조방법에 따른 작약의 성분적 품질평가의 한 요인이 되는 엑스 함량을 검토하고 아울러 작약의 활성효과 중 항염증 작용 및 위액분비 억제 작용에 관여하는 것으로 보고된 엑스의 pH 변화 양상을 검토한 결과를 요약하면 다음과 같다. 1. 생육년수의 차이에 따른 엑스 함량 및 pH변화를 검토한 결과 유피근, 거피근 모두 생육년수에 따른 통계적 차이를 나타내지 않았다. 한편 $2{\sim}4$년생 작약의 유피근 함유엑스 함량이 거피근보다 $3.7%{\sim}9.2%$ 높은 양상을 나타내므로 작약근 껍질부위에도 엑스 추출 시 용출되는 물질이 다량 존재함을 알 수 있다. 2. 4년생 의성작약, 영천작약 및 좀작약을 각각 유피근과 거피근으로 가공하고 상온에서 음건한 후 각 품종별 엑스함량 및 엑스의 pH변화를 조사한 결과 엑스 함량의 경우 유피근, 거피근 모두 품종 간 차이를 나타내었고, 영천작약이 유피근, 거피근 각각 36%, 30% 수준으로 의성 작약 및 좀작약 보다 높은 엑스 함량을 나타내었으며, 엑스의 pH는 품종 간 차이가 인정 되지 않았다. 3. 4년생 의성작약을 대상으로 건조방법에 따른 엑스 함량 및 엑스의 pH 변화를 검토한 결과 유피근, 거피근 모두 건조방법 간에 뚜렷한 차이를 나타내었다. 엑스 함량은 유피근의 경우 상온 음건이, 거피근은 $80^{\circ}C$ 수증건법 이 가장 높았고, 동결건조법은 유피근, 거피근 모두 가장 낮은 함량을 나타내었다. 엑스의 pH변화는 유피근, 거피근 모두 냉동건조법이 pH 5.1 수준으로 가장 높았고, $80^{\circ}C$ 수증건법이 유피근, 거피근 모두 pH 3.7 수준으로 가장 낮은 양상을 나타내었다.