• 인터넷정보학회논문지
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• 제20권3호
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• pp.77-84
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• 2019

Workflow management system is a system that manages the workflow model which defines the process of work in reality. We can define the workflow process by sequencing jobs which is performed by the performers. Using the workflow management system, we can also analyze the flow of the process and revise it more efficiently. Many researches are focused on how to make the workflow process model more efficiently and manage it more easily. Recently, many researches use the workflow log files which are the execution history of the workflow process model performed by the workflow management system. Ourresearch group has many interests in making useful knowledge from the workflow event logs. In this paper we use XES log files because there are many data using this format. This papersuggests what are the cardinalities of the temporal workcases and how to get them from the workflow event logs. Cardinalities of the temporal workcases are the occurrence pattern of critical elements in the workflow process. We discover instance cardinalities, activity cardinalities and organizational resource cardinalities from several XES-based workflow event logs and visualize them. The instance cardinality defines the occurrence of the workflow process instances, the activity cardinality defines the occurrence of the activities and the organizational cardinality defines the occurrence of the organizational resources. From them, we expect to get many useful knowledge such as a patterns of the control flow of the process, frequently executed events, frequently working performer and etc. In further, we even expect to predict the original process model by only using the workflow event logs.

• Animal Bioscience
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• 제34권1호
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• pp.134-142
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• 2021

Objective: To understand the athletic characteristics of Thoroughbreds, high-throughput analysis has been conducted using horse muscle tissue. However, an in vitro system has been lacking for studying and validating genes from in silico data. The aim of this study is to validate genes from differentially expressed genes (DEGs) of our previous RNA-sequencing data in vitro. Also, we investigated the effects of exercise-induced stress including heat, oxidative, hypoxic and cortisol stress on horse skeletal muscle derived cells with the top six upregulated genes of DEGs. Methods: Enriched pathway analysis was conducted using the Database for Annotation, Visualization, and Integrated Discovery (DAVID) tool with upregulated genes in horse skeletal muscle tissue after exercise. Among the candidates, the top six genes were analysed through geneMANIA to investigate gene networks. Muscle cells derived from neonatal horse skeletal tissue were maintained and subjected to exercise-related stressors. Transcriptional changes in the top six genes followed by stressors were investigated using quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Results: The inflammation response pathway was the most commonly upregulated pathway after horse exercise. Under non-cytotoxic conditions of exercise-related stressors, the transcriptional response of the top six genes was different among types of stress. Oxidative stress yielded the most similar expression pattern to DEGs. Conclusion: Our results indicate that transcriptional change after horse exercise in skeletal muscle tissue strongly relates to stress response. The qRT-PCR results showed that stressors contribute differently to the transcriptional regulation. These results would be valuable information to understand horse exercise in the stress aspect.

• 한국동물위생학회지
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• 제44권4호
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• pp.185-194
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• 2021

Polyglutamine extension in the coding sequence of mutant huntingtin causes neuronal degeneration associated with the formation of insoluble polyglutamine aggregates in Huntington's disease (HD). Mutant huntingtin can form aggregates within the nucleus and processes of neurons possibly due to misfolding of the proteins. To better understand the mechanism by which an elongated polyglutamine causes aggregates, we have developed an in vitro binding assay system of polyglutamine tract from truncated huntingtin. We made GST-HD exon1 fusion proteins which have expanded polyglutamine epitopes (e.g., 17, 23, 32, 46, 60, 78, 81, and 94 CAG repeats). In the present emergence of new study adjusted nanotechnology on protein chip such as surface plasmon resonance strategy which used to determine the substance which protein binds in drug discovery platform is worth to understand better neurodegenerative diseases (i.e., Alzheimer disease, Parkinson disease and Huntington disease) and its pathogenesis along with development of therapeutic measures. Hence, we used strengths of surface plasmon resonance (SPR) technology which is enabled to examine binding specificity and explore targeted molecular epitope using its electron charged wave pattern in HD pathogenesis utilize conjugated mutant epitope of HD protein and its interaction whether wild type GST-HD interacts with mutant GST-HD with maximum binding affinity at pH 6.85. We found that the maximum binding affinity of GST-HD17 with GST-HD81 was higher than the binding affinities of GST-HD17 with other mutant GST-HD constructs. Furthermore, our finding illustrated that the mutant form of GST-HD60 showed a stronger binding to GST-HD23 or GST-HD17 than GST-HD60 or GST-HD81. These results indicate that the binding affinity of mutant huntingtin does not correlate with the length of polyglutamine. It suggests that the aggregation of an expanded polyglutamine might have easily occurred in the presence of wild type form of huntingtin.

• 한국정보통신학회논문지
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• 제26권8호
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• pp.1148-1157
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• 2022

2019년 코로나로 인하여 사람들의 많은 생활에 변화를 주었다. 그중 메타버스는 다양한 방식을 통한 비대면 서비스를 지원하여 일상에서 하던 일을 대체하고 있다. 이런 현상은 코로나19의 장기화로 하나의 문화처럼 만들어지고 형성되었다. 본 논문은 메타버스의 재미요인을 알기 위해 기존 게임에서 사용한 재미요소를 정리하여 전문가 5명과 함께 항목, 내용을 메타버스에 맞게 재분류하였다. 분류는 재매개성을 사용하여 분류하였고 감각적 재미[시각(그래픽), 청각, 텍스트, 조작, 감정이입, 유희, 시점], 도전적 재미[몰입, 도전, 성취, 발견, 스릴, 보상, 문제해결], 상상적 재미[새로운 이야기, 사랑, 자유도, 대리자아, 기대감, 변화], 사회적 재미[규칙, 경쟁, 사회적 행위, 지위, 협동, 참여, 교류, 소속, 화폐거래], 상호작용적 재미[의사결정, 커뮤니케이션 공유, 하드웨어, 감정이입, 육성, 자율성], 현실적 재미[현실 일체감, 학습 용이성, 순응, 지적문제해결, 패턴 인식, 실재감, 커뮤니티], 창조적 재미[응용, 창조, 커스텀마이징, 가상세계]로 구분하였다.

• International Journal of Industrial Entomology and Biomaterials
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• 제47권1호
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• pp.51-62
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• 2023

The advantages of silkworms as functional foods are well known and various products are being developed. In general, silkworms sold in the market include silkworm powder (3 days of fifth instars) and SukJam (7 days or more of fifth instars), In other words, product classification is made according to the maturity of the fifth instar silkworms. In this study, we analyzed the gene expression changes in the fifth instar silkworms and attempted to validate the use of deregulated genes in maturity analysis. After rearing BaekokJam, transcriptome analysis was performed on days 1, 3, 5, and 8 days of the fifth instar, and differentially expressed genes showing differences at each period were selected. Of the 31,841 contigs analyzed, 4012 contigs were identified with a log2 fold change of two or more between 5 and 8 days of the fifth instar. RT-PCR was performed for 18 contigs, which showed increased or decreased expression, but in c127159, c97909, c96974, c119920, c42251, and c80216 showed clear differences. To identify SukJam, a combination of the contigs c127159 (180 bp), c97909 (143 bp), and c80216 (120 bp) was amplified. Taken together, these results suggest that the harvest time of silkworms can be determined using gene expression pattern analysis.

• 생명과학회지
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• 제22권11호
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• pp.1552-1557
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• 2012

Pipernonaline은 후추나무과에 속하는 필발(Piper longum Linn.)의 유도체로서 전립선 암세포에 대한 항암활성이 보고되고 있다. 하지만 실제 암세포 내에서 생물학적 정보를 가진 수 많은 유전자들에 대한 발현이 어떻게 이루어지고 있는지 알려진 바가 없다. 본 연구에 사용된 microarray 분석은 동시에 수 만개 이상의 유전자 발현양상을 한번에 관찰할 수 있는 기술로서 특정 질병의 유전학적 특성과 기전 연구를 더 광범위하게 연구 할 수 있는 기술이다. 본 연구에서는 전립선 암세포인 PC-3 세포에 pipernonaline을 처리하여 cDNA microarray를 실시하였다. 이후, DAVID database를 이용하여 gene ontology의 Biological Process를 분석하여 세포사멸과 세포주기, 세포성장 및 증식에 관련된 유전자들을 우선적으로 분석하였다. 그 결과, 세포주기관련 256개, 세포사멸관련 197개, 세포성장 및 증식관련에 154개의 유전자가 확인 되었다. 이러한 결과는 pipernonaline은 전립선 암세포 내에 존재하는 생물학적 신호전달체계에 관련된 유전자 발현을 조절함으로써 항암활성을 나타내 것을 알 수 있었고, 이후 이러한 microarray의 추가적인 분석은 암세포 내 새로운 유전자의 탐색 및 메커니즘을 규명하는데 유용하게 사용할 수 있을 것으로 사료된다.

• 핵의학기술
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• 제15권2호
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• pp.41-46
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• 2011

영상장치를 이용한 검사 시 영상의 질은 검사결과와 밀접하게 연관되어 있으며, 이는 영상획득 조건들과 이에 대한 평가방법으로 인해 달라 질 수 있다. 본 연구에서는 영상획득 시 사전설정법(Pre-set method)차이에 따른 영상의 질을 평가하였다. 장비는 PET/CT Discovery STe16(GE Healthcare, Milwaukee, USA)을 사용하였으며, Chest PET Phantom (실험 1)과 94 NEMA Phantom (실험 2)을 이용하였다. 실험 1의 경우 3.5, 6.0, 8.6 kBq/mL, 실험 2의 경우는 3.3, 5.5, 7.7, 9.9, 12.1, 16.5 kBq/mL의 $^{18}F$-FDG를 채웠고, 열소와 배후방사능과의 방사능 농도는 4:1의 비율을 유지하였다. 각 실험은 CT 투과촬영 후 3D로 2분 30초/프레임으로 시간설정법(Time-set method)과 1억 계수의 계수설정법 (Count-set method)을 적용하여 방출촬영 하였다. 영상의 질에 대한 평가는 잡음 등가 계수율 (Noise Equivalent Count Rate, NECR)과 신호 대 잡음비 (Signal to Noise Ratio, SNR)를 이용하였다. 실험 1에서 NECR과 SNR은 방사능 농도의 증가에 따라 시간설정법에서 53.7, 66.9, 91.4 과 7.9, 10.0, 11.7로 평가 모두에서 증가함을 보였으나, 계수설정법은 53.8, 69.1, 97.8과 14.1, 14.7, 14.4로 SNR은 NECR과 다르게 증감현상을 보이지 않았다. 또 다른 모형실험인 실험 2에서도 NECR과 SNR은 방사능 농도가 증가에 따라 시간설정법에서 45.1, 70.6, 95.3 115.6 134.6 162.2 과 7.1, 8.8, 10.6, 11.5, 12.7, 14.0으로 증가함을 보였으나, 계수설정법에서는 42.1 67.3 92.1 112.2 130.7 158.7 과 15.2, 15.9, 15.6, 15.4, 15.5, 14.9로 실험 1에서와 마찬가지로 SNR에서는 증감현상을 보이지 않았다. 사전설정법 변화와 관계없이 단위 질량당 방사능량 증가는 영상의 질도 비례하여 향상된다. 그러나 동일한 단위 질량당 방사능량에서는 영상획득의 시간을 늘려 총계수값을 증가시켜도 NECR에 영향을 주지는 않는다. 이는 NECR을 이용한 영상 질 평가는 영상획득 시 얻은 총 계수 값보다는 단위 시간당의 계수율이 영향을 준다는 것을 의미한다고 할 수 있다. 실험 모두에서 계수설정법으로 얻은 경우에는 단위 질량당 방사능량의 증가에도 불구하고 거의 비슷한 SNR값을 보이게 된다. 따라서 좋은 질의 영상을 얻기 위해서는 총 계수값의 증가보다는 단위질량당 방사능량을 높여야 하며, SNR만을 이용한 영상의 질에 대한 평가는 적절하지 않을 수 있다는 점을 고려하여야 한다.

• 대한치과교정학회지
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• 제27권5호
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• pp.669-682
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• 1997

[$Bj\ddot{o}rk$]와 Skieller는 상, 하악골에 임플란트를 매식하여 안면골 성장유형을 관찰한 결과 성장으로 인하여 영향을 받지 않는 안정된 골구조물(stable structure)의 존재를 확인했고 성장중에 있는 환자의 안면골을 중첩할 때에는 반드시 이러한 부위를 기준으로 중첩해야 한다고 하였다. 그럼에도 불구하고 40여년이 흐른 지금까지 대부분의 교정의들은 안정골구조물을 이용한 중첩법(structural method) 보다는 최적중첩법(best fit method)을 선호하고 있다. 그러나 Angle씨 부정교합 II급 2류의 하악골과 같이 특징적으로 전상방 성장을 하는 골격구조를 갖는 증례와, 치료 후 장기관찰 증례에서는 두 중첩법 사이에 현저한 차이가 있다는 사실을 부인할 수는 없다. 이제는 윤리적인 문제때문에 더이상 임플란트를 매식하여 관찰하는 연구를 할 수 없게 되어 $Bj\ddot{o}rk$가 연구해왔던 임플란트 자료를 근거로 안정골구조물 중첩법을 사용할 수 밖에 없다. 따라서 안정골구조물 중첩법의 실용화를 위하여 본 저자들은 안정골구조물 중첩법의 합리적 근거를 제시하고 중첩방법을 자세히 소개하고자 한다.

• 한국재료학회지
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• 제21권4호
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• pp.216-219
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• 2011

Perovskite manganites such as $RE_{1-x}A_xMnO_3$ (RE = rare earth, A = Ca, Sr, Ba) have been the subject of intense research in the last few years, ever since the discovery that these systems demonstrate colossal magnetoresistance (CMR). The CMR is usually explained with the double-exchange (DE) mechanism, and CMR materials have potential applications for magnetic switching, recording devices, and more. However, the intrinsic CMR effect is usually found under the conditions of a magnetic field of several Teslas and a narrow temperature range near the Curie temperature ($T_c$). This magnetic field and temperature range make practical applications impossible. Recently, another type of MR, called the low-field magnetoresistance(LFMR), has also been a research focus. This MR is typically found in polycrystalline half-metallic ferromagnets, and is associated with the spin-dependent charge transport across grain boundaries. Composites with compositions $La_{0.7}(Ca_{1-x}Sr_x)_{0.3}MnO_3)]_{0.99}/(BaTiO_3)_{0.01}$ $[(LCSMO)_{0.99}/(BTO)_{0.01}]$were prepared with different Sr doping levels x by a standard ceramic technique, and their electrical transport and magnetoresistance (MR) properties were investigated. The structure and morphology of the composites were studied by X-ray diffraction (XRD) and scanning electronic microscopy (SEM). BTO peaks could not be found in the XRD pattern because the amount of BTO in the composites was too small. As the content of x decreased, the crystal structure changed from orthorhombic to rhombohedral. This change can be explained by the fact that the crystal structure of pure LCMO is orthorhombic and the crystal structure of pure LSMO is rhombohedral. The SEM results indicate that LCSMO and BTO coexist in the composites and BTO mostly segregates at the grain boundaries of LCSMO, which are in accordance with the results of the magnetic measurements. The resistivity of all the composites was measured in the range of 90-400K at 0T, 0.5T magnetic field. The result indicates that the MR of the composites increases systematically as the Ca concentration increases, although the transition temperature $T_c$ shifts to a lower range.

• 한국작물학회:학술대회논문집
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• 한국작물학회 2017년도 9th Asian Crop Science Association conference
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• pp.35-35
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• 2017

How do plants take up water from soils especially when water is scarce in soils? Plants have a strategy to respond to water deficit to manage water necessary for their survival and growth. Plants regulate water transport inside them. Water flows inside the plant via (i) apoplastic pathway including xylem vessel and cell wall and (ii) cell-to-cell pathway including water channels sitting in cell membrane (aquaporins). Water transport across the root and leaf is explained by a composite transport model including those pathways. Modification of the components in those pathways to change their hydraulic conductivity can regulate water uptake and management. Apoplastic barrier is modified by producing Casparian band and suberin lamellae. These structures contain suberin known to be hydrophobic. Barley roots with more suberin content from the apoplast showed lower root hydraulic conductivity. Root hydraulic conductivity was measured by a root pressure probe. Plant root builds apoplastic barrier to prevent water loss into dry soil. Water transport in plant is also regulated in the cell-to-cell pathway via aquaporin, which has received a great attention after its discovery in early 1990s. Aquaporins in plants are known to open or close to regulate water transport in response to biotic and/or abiotic stresses including water deficit. Aquaporins in a corn leaf were opened by illumination in the beginning, however, closed in response to the following leaf water potential decrease. The evidence was provided by cell hydraulic conductivity measurement using a cell pressure probe. Changing the hydraulic conductivity of plant organ such as root and leaf has an impact not only on the speed of water transport across the plant but also on the water potential inside the plant, which means plant water uptake pattern from soil could be differentiated. This was demonstrated by a computer simulation with 3-D root structure having root hydraulic conductivity information and soil. The model study indicated that the root hydraulic conductivity plays an important role to determine the water uptake from soil with suboptimal water, although soil hydraulic conductivity also interplayed.