• 한국식물병리학회:학술대회논문집
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• 한국식물병리학회 2003년도 정기총회 및 추계학술발표회
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• pp.78.2-79
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• 2003

Pepper defensin ( CADEFl) clone was isolated from cDNA library constructed from pepper leaves infected with avirulent strain Bv5-4a of Xanthomonu campestris pv. vesicatoria. The deduced amino acid sequence of CADEFl is 82-64％ identical to that of other plant defensins. Putative protein encoded by CADEFl gene consists of 78 amino acids and 8 conserved cysteine residues to form four structure-stabilizing disulfide bridges. Transcription of the CADEF1 gene was earlier and stronger induced by X campestris pv. vesicatoria infection in the incompatible than in the compatible interaction. CADEF1 mRNA was constitutively expressed in stem, root and green fruit of pepper. Transcripts of CADEFl gene drastically accumulated in pepper leaf tissues treated With Salicylic acid (SA), methyl jasmonate (MeJA), abscisic acid (ABA), hydrogen Peroxide (H$_2$O$_2$), benzothiadiazole (BTH) and DL-${\beta}$-amino-n-butyric acid (BABA). In situ hybridization results revealed that CADEF1 mRNA was localized in the phloem areas of vascular bundles in leaf tissues treated with exogenous SA, MeJA and ABA. Strong accumulation of CADEF1 mRNA occurred in pepper leaves in response to wounding, high salinity and drought stress. These results suggest that bacterial pathogen infection, abiotic elicitors and some environmental stresses may play a significant role in signal transduction pathway for CADEF1 gene expression.

• Journal of Microbiology and Biotechnology
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• 제18권3호
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• pp.397-403
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• 2008

Nikkomycins are a group of peptidyl nucleoside antibiotics with potent fungicidal, insecticidal, and acaricidal activities. sanN was cloned from the partial genomic library of Streptomyces ansochromogenes 7100. Gene disruption and complementation analysis demonstrated that sanN is essential for nikkomycin biosynthesis in S. ansochromogenes. Primer extension assay indicated that sanN is transcribed from two promoters (sanN-P1 and sanN-P2), and sanN-P2 plays a more important role in nikkomycin biosynthesis. Purified recombinant SanN acts as a dehydrogenase to convert benzoate-CoA to benzaldehyde in a random-order mechanism in vitro, with respective $K_{cat}/K_m$$ values of $3.8mM^{-1}s^{-1}\;and\;12.0mM^{-1}s^{-1}$ toward benzoate-CoA and NADH, suggesting that SanN catalyzes the formation of picolinaldehyde during biosynthesis of nikkomycin X and Z components in the wild-type stain. These data would facilitate us to understand the biosynthetic pathway of nikkomycins and to consider the combinatorial synthesis of novel antibiotic derivatives.

• 한국산학기술학회논문지
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• 제11권10호
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• pp.3982-3987
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• 2010

산신은 우리나라의 전통적 토착 신앙이 불교 내부에 수용된 것으로 사찰 안에 산신각이 건립된 것은 17세기 이후에 시작된 것으로 본다. 불교 고유의 신격이 아닌 까닭에 산신각의 입지는 불전의 배후에 떨어져 산과 가까운 기슭에 위치하고 있는 것이 대부분이다. 대다수의 사찰에서 산신각에 중요한 의미를 부여하고 있음에도 그 크기가 작은 까닭은 산신 신앙의 개인성에서 비롯한 것이며 또한 이러한 간결한 형태를 통하여 산천 정기가 집약, 응축된 장소임을 강하게 표현하려는 상징적 의도가 나타난 것이라고 할 수 있다. 산신각은 산천의 정기가 사찰 안으로 흘러 모여드는 통로이자 불교의 고유한 신앙체계가 무한한 자연의 영역으로 확장되는 출구이기도 하다.

• 한국사회복지학
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• 제42권
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• pp.169-198
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• 2000

This paper attempts to analyze the early retirement in the OECD countries and discuss implication of that in the old age policy in Korea. The increase of the early retirement in the almost all OECD countries is a common fact. Especially the rate of early retirement rapidly increased in the 1980s, mostly reflecting the high rate of unemployment and states' policies to reduce it. However, it varies across countries: the unemployment compensation pathway in France, the mixture of social assistance and private insurance in England, VUT in Netherland, the privatization of the early retirement in the U. S., and partial retirement and labor market policy in Sweden. The early retirement in the advanced countries contributes to de-institutionalization and de-standardization in life course model. It resulted in the erosion of the ordinary conception that the retirement was the beginning of the old age. And the last phase of life course became blurred. With respect to the problem of the early retirement, there is a big difference between Korea and the OECD countries. Above all, the retirement age is 55 years in many companies and the public pension is not universalized in Korea. Accordingly the policy for income security of the old age in Korea should be connected with social security policy such as the gradual extension of the retirement age and the expansion of the public pension and labor market policy such as job training for the old age, transformation of the seniority wage system etc.

• Journal of Acupuncture Research
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• 제36권4호
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• pp.197-203
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• 2019

The purpose of this study was to investigate useful manual therapy techniques for peripheral facial nerve palsy and to propose guidelines to be applied for current manual therapy techniques. Several databases were searched to find manual therapies for facial palsy. These therapies included cervical, and temporomandibular joint chuna manual therapy, proprioceptive neuromuscular facilitation, neuromuscular re-education, facial exercise, and mime therapy. Both cervical, and temporomandibular joint chuna manual therapy release nerve compression, helping blood circulation and nerve conduction. Proprioceptive neuromuscular facilitation uses irradiation, bilateral activation, and eccentric facilitation to improve muscle power and symmetry. Neuromuscular re-education, as a retraining tool for facial movement patterns, enhances neuromuscular feedback. Facial exercise helps the patient continuously move and massage facial muscle themselves. Mime therapy aims to develop a conscious connection between the use of certain muscles and facial expressions. The use of facial chuna manual therapy for peripheral facial nerve palsy can stimulate the proprioceptive neuromuscular receptors in the face. Peripheral facial nerve palsy has 4 phases; progress phase, plateau phase, recovery phase, and sequelae phase. Each phase needs different treatments which include relaxation, assistance, resistance, origin-insertion extension, and nerve pathway expansion.

• Molecules and Cells
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• 제42권8호
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• pp.597-603
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• 2019

Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is a core enzyme of the aerobic glycolytic pathway with versatile functions and is associated with cancer development. Recently, Kornberg et al. published the detailed correlation between GAPDH and di- or monomethyl fumarate (DMF or MMF), which are well-known GAPDH antagonists in the immune system. As an extension, herein, we report the crystal structure of MMF-bound human GAPDH at $2.29{\AA}$. The MMF molecule is covalently linked to the catalytic Cys152 of human GAPDH, and inhibits the catalytic activity of the residue and dramatically reduces the enzymatic activity of GAPDH. Structural comparisons between $NAD^+$-bound GAPDH and MMF-bound GAPDH revealed that the covalently linked MMF can block the binding of the $NAD^+$ cosubstrate due to steric hindrance of the nicotinamide portion of the $NAD^+$ molecule, illuminating the specific mechanism by which MMF inhibits GAPDH. Our data provide insights into GAPDH antagonist development for GAPDH-mediated disease treatment.

• BMB Reports
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• 제52권1호
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• pp.24-34
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• 2019

Sirtuin is an essential factor that delays cellular senescence and extends the organismal lifespan through the regulation of diverse cellular processes. Suppression of cellular senescence by Sirtuin is mainly mediated through delaying the age-related telomere attrition, sustaining genome integrity and promotion of DNA damage repair. In addition, Sirtuin modulates the organismal lifespan by interacting with several lifespan regulating signaling pathways including insulin/IGF-1 signaling pathway, AMP-activated protein kinase, and forkhead box O. Although still controversial, it is suggested that the prolongevity effect of Sirtuin is dependent with the level of and with the tissue expression of Sirtuin. Since Sirtuin is also believed to mediate the prolongevity effect of calorie restriction, activators of Sirtuin have attracted the attention of researchers to develop therapeutics for age-related diseases. Resveratrol, a phytochemical rich in the skin of red grapes and wine, has been actively investigated to activate Sirtuin activity with consequent beneficial effects on aging. This article reviews the evidences and controversies regarding the roles of Sirtuin on cellular senescence and lifespan extension, and summarizes the activators of Sirtuin including Sirtuin-activating compounds and compounds that increase the cellular level of nicotinamide dinucleotide.

• Investigative Magnetic Resonance Imaging
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• 제4권1호
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• pp.14-19
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• 2000

목적 : 뇌열환자에서 기능적 자기공명영상과 경두부 자기자극을 이용하여 뇌기능을 지도화하고자하였다. 대상 및 방법: 자기공명영상에서 우측 대뇌반구에 뇌열 소견이 있으며 좌측 편측부전마비를 보인 28세 남자환자를 대상으로 기능적 자기공명영상과 경두부 자기자극을 시행하였다. 임상적으로 좌측 손의 운동기능은 감소되어 있었고, 우측 손의 운동기능은 정상범주에 속하였다. 뇌기능적 자기공명영상은 EPI 기법을 이용하였고 운동자극은 1-2 Hz의 주기로 손가락을 아래위로 구부리게 하는 운동을 시행하였고 15초의 휴식기와 15초의 운동기를 반복하여 절편당 60 개의 영상을 획득하였다. 두부자기자극은 지름 90mm의 원형 자성자극기를 이용하여 maximal out-put의 80%로 자극하여 양측 단무지 외 전근에서 유발된 운동유발전위의 잠시와 진폭을 구하였다. 결과: 기능적 자기공명영상을 시행한 결과 정상적인 우측손의 운동자극시에 좌측 운동피질이 활성화되었고 좌측손의 운동자극시에는 좌측운동피질, 좌측 부가운동영역, 그리고 자측 전운동영역에 활성화소견이 나타났다. 두부자기자극에서는 우측 대뇌반구에서는 한군데에서도 운동유발 전위가 발생되지 않았다. 좌측 대뇌반구에서는 5군데에서 운동유발전위가 유발되었으며 모두 양측 단무지 외 전근에서 운동유발전위가 유발되었다. 양손에서 운동유발전위의 잠시 , 진폭 모양이 유사하였다. 결론: 뇌열환자의 손운동기능의 피지도화는 기능적 자기공명영상과 두부자기자극을 이용하여 성공적으로 시행할 수 있었다. 뇌열환자의 동측 운동경로는 동일한 운동피질로부터 기원한 동측피질척수로에 의한 것으로 추정된다.

• 한국건설관리학회논문집
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• 제18권2호
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• pp.81-90
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• 2017

임진강 유역은 1996년부터 1999년까지 3번의 대규모 홍수가 발생하여 많은 인명피해와 9천억원의 재산피해를 입었다. 우리나라는 기후변화로 인하여 홍수피해가 앞으로 증가할 것으로 예상된다. 본 연구는 기후시나리오를 활용하여 미래의 홍수피해를 예측하고, 실물옵션 기반 경제성분석 방법을 제시하였으며, 임진강유역의 홍수방지시설물 투자사업의 사례연구를 통해 경제성분석을 실시하였다. RCP (Representative Concentration Pathway) 기후시나리오에서 모의된 강수량 자료를 활용하여 홍수피해액을 계산하고, 홍수방지시설물 투자에 의한 저감이익을 분석하였다. 향후 RCP8.5와 RCP4.5 기후시나리오가 실현되는 조건을 가정하여 홍수피해 저감이익의 변동성을 구하고, 2071년에 200년 재현주기에 적응하도록 하는 확장을 위한 투자를 할 수 있는 확장옵션을 적용하여 프로젝트의 옵션가치를 구했다. 옵션가치 분석결과, 두 가지 시나리오 하에서 경제성을 확보하고 있음을 확인하였고, RCP8.5 기후시나리오가 실현될 때가 RCP4.5의 경우보다 홍수피해 저감이익이 더 많이 발생하였다. 본 연구는 정부 의사결정권자가 실물옵션분석방법을 활용하여 홍수방재시설물의 경제성분석에 기후변화 불확실성을 고려할 수 있도록 도와줄 것으로 기대되며, 기후시나리오에서 제공하는 강우자료를 활용하여 기후위험요소를 경제적 가치로 정량화하는 방법을 제시하였다.

• Journal of Preventive Medicine and Public Health
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• 제24권4호
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• pp.485-495
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• 1991

This study examined the effects of referral requirements for insurance patients which have been enforced since July 1, 1989 when medical insurance coverage was extended to the whole population except beneficiaries of medical assistance program. The requirements are mainly aimed at discouraging the use of tertiary care hospitals by imposing restrictions on the patient's choice of a medical service facility. The expectation is that such change in the pattern of medical care utilization would produce several desirable effects including increased efficiency in patient care and balanced development of various types of medical service facilities. In this study, these effects were assessed by the change in the number of out-patient visits and bed-days per illness episode and the share of each type of facility in the volume of services and the amount of expenditures after the implementation of the new referral system. The data for analysis were obtained from the claims to the insurance for government and school employees. The sample was drawn from the claims for the patients treated during the first six months of 1989, prior to the enforcement of referral requirements, and those of the patients treated during the first six months of 1990, after the enforcement. The 1989 sample included 299,824 claims (3.6% of total) and the 1990 sample included 332,131 (3.7% of total). The data were processed to make the unit of analysis an illness episode instead of an insurance claim. The facilities and types of care utilized for a given illness episode are defined to make up the pathway of medical care utilization. This pathway was conceived of as a Markov Chain process for further analysis. The conclusion emerged from the analysis is that the enforcement of referral requirements resulted in less use of tertiary care hospitals, and thereby decreased the volume of services and the amount of insurance expenses per illness episode. However, there are a few points that have to be taken into account in relation to the conclusion. The new referral system is likely to increase the use of medical services not covered by insurance, so that its impact on national health expenditures would be different from that on insurance expenditures. The extension of insurance coverage must have inereased patient load for all types of medical service organizations, and this increase may be partly responsible for producing the effects attributed to the new referral system. For example, excessive patient load for tertiary care hospitals may lead to the transfer of their patients to other types of facilities. Another point is that the data for this study correspond to very early phase of the new system. But both patients and medical care providers would adapt themselves to the new system to avoid or overcome its disadvantages for them, so as that its effects could change over time. Therefore, it is still necessary to closely monitor the impact of the referral requirements.