• 한국산림과학회지
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• 제104권4호
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• pp.578-587
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• 2015

교배양식 유전모수를 확인하기 위하여 nuclear SSR (nSSR) 표지와 chloroplast SSR (cpSSR) 표지를 이용하여 2006년과 2007년에 안면도 소나무 채종원(77년 조성)에서 생산된 종자를 대상으로 타가교배율과 화분오염율, 근친교배율을 확인하였다. cpSSR 유전형에 근거한 타가교배율은 2006년에 94.9~100%(평균 98.9%)이며, 2007년에는 91.2~100%(평균 97.7%)이다. nSSR 유전자형에 근거한 타가교배율은 2006년에 90.3~100%(평균 95.9%), 2007년에 81.6~100%(평균 95.3%)의 타가교배율이 산출되었다. 두 표지를 동시에 비교하여 확인한 결과 2006년 생산종자의 평균 누적 타가교배율 100%, 2007년 생산종자의 평균 누적 타가교배율은 98.9%로 추정되었다. 근친교배율($t_m-t_s$: biparental inbreeding)은 2006년에 -0.006과 2007년에 0.007으로 추정되었다. 평균 화분오염율은 2006년에 평균 48.9%, 2007년에 평균 42.4%이며, 종자의 cpSSR 유전형을 근거로 확인한 화분친 기여율(기여화분친 수)은 2006년에 0.458(평균 16.2개), 2007년에 0.512(평균 14.8개)로 확인되었다. 결론적으로, 2006년, 2007년 안면도 소나무 채종원(77년 조성) 내 클론간 높은 타가교배율이 확인됨으로써 채종원산 종자의 유전적 품질은 자가교배로 인한 근교약세가 원인이 되는 불량형질이 발생할 가능성이 낮을 것으로 기대된다. 안면도 소나무 채종원(77년 조성) 내 교배양식 연간 분석을 통해서 확인된 결과가 향후 진전세대 채종원 조성 및 관리에 유용한 정보를 제공할 것으로 기대된다.

• Asian Pacific Journal of Cancer Prevention
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• 제16권16호
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• pp.6967-6972
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• 2015

Background: Cigarette smoking and tobacco chewing are common modes of consuming tobacco all over the world. Parents need to be aware that germ cell integrity is vital for birth of healthy offspring as biological parenting begins much before birth of a child and even before conception. The present study was conducted to determine the etiology of non-familial sporadic heritable retinoblastoma (NFSHRb), by evaluating oxidative sperm DNA damage in fathers due to use of tobacco (smoking and chewing). Materials and Methods: We recruited 145 fathers of NFSHRb children and 53 fathers of healthy children (controls) in the study. Tobacco history was obtained by personal interview. Seminal reactive oxygen species (ROS) in semen, sperm DNA fragmentation index (DFI) and 8 hydroxy 2' deoxyguanosine (8-OHdG) levels in sperm were evaluated. The RB1 gene was screened in genomic blood DNA of parents of children with NFSHRb and controls. Odds ratios (ORs) derived from conditional logistic regression models. Results: There was significant difference in the levels of ROS (p<0.05), DFI (p<0.05) and 8-OHdG (p<0.05) between tobacco users and non-users. The OR of NFSHRb for smokers was 7.29 (95%CI 2.9-34.5, p<0.01), for tobacco chewers 4.75 (2.07-10.9, p<0.05) and for both 9.11 (3.79-39.2; p<0.01). Conclusions: This study emphasizes the adverse effect of tobacco on the paternal genome and how accumulation of oxidative damage in sperm DNA may contribute to the etiology of NFSHRb. In an ongoing parallel study in our laboratory, 11 of fathers who smoked underwent. Meditation and yoga interventions, showed significant decline in levels of highly mutagenic oxidised DNA adducts after 6 months. Thus our lifestyle and social habits impact sperm DNA integrity and simple interventions like yoga and meditation are therapeutic for oxidative damage to sperm DNA.

• Nutrition Research and Practice
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• 제8권6호
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• pp.705-712
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• 2014

BACKGROUND/OBJECTIVES: Dietary guidelines for Korean children were released in 2009. The goal of the present study was to examine diet quality in terms of adherence to these dietary guidelines as well as explore the association between guideline adherence and risk of obesity in Korean children. SUBJECTS/METHODS: Children aged 5-11 years (mean age = 8.9 years old, n = 191, 80.6% girls) were recruited from a university hospital in Seoul, Korea. Adherence to dietary guidelines for Korean children was calculated using the Likert scale (1-5), and children were then categorized into low, moderate, and high groups based on adherence scores. Obesity or being overweight was determined based on an age- and gender-specific percentile for body mass index (BMI) of the 2007 Korean National Growth Charts. Diet quality was evaluated from 3 days of dietary intake data. RESULTS: Children in the high adherence group were characterized by significantly lower BMI percentiles and paternal BMIs as well as higher percentages of fathers with a high level of education and higher household incomes compared to those in the low or moderate group. Children in the high adherence group consumed significantly higher amounts of milk and dairy products, were less likely to consume lower than the EAR of phosphorus and iron, and had higher NARs for calcium, phosphorus, iron, zinc, and MAR than those in low groups. The ORs for obesity ($BMI{\geq}95^{th}$ percentile) or being overweight including obesity ($BMI{\geq}85^{th}$ percentile) were significantly lower in the high adherence group compared to the low adherence group (OR: 0.33, 95% CI = 0.13-0.82, P for trend = 0.019; OR: 0.26, 95% CI: 0.11-0.61 P for trend = 0.002). CONCLUSIONS: Korean children who adhered to dietary guidelines displayed better diet quality and a reduced risk of obesity.

• Journal of Plant Biotechnology
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• 제44권1호
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• pp.35-41
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• 2017

본 연구에서는 유지형 배추인 LP08과 청경채형 LP21을 교배한 교잡종 $F_1$을 소포자 배양하여 유전적으로 고정된 계통을 획득하였다. 엽의 결각 유무 및 결각수에서 다양한 형태적 특징을 보이는 66개 고정계통을 대상으로 항암 및 항산화 물질로 알려진 플라보노이드의 함량을 분석하였다. 66개 고정 계통의 엽의 결각 유무와 플라보노이드 함량 간 상관관계는 관찰되지 않았다. 양친으로 사용된 LP08, LP21 그리고 결구 배추형 품종인 Chiifu를 대상으로 플라보노이드 분석을 실시한 결과, quercetin, naringenin, 및 kaempferol 함량의 다양한 분포가 관찰되었다. 또한 모계인 LP08에서 가장 높은 총 플라보노이드 함량을 나타내었고 결구 배추형인 Chiifu에서 가장 낮은 함량을 보였다. 66개 고정 계통을 대상으로 플라보노이드 함량을 분석한 결과, quercetin은 LP08 보다 높은 함량을 보이는 18 계통이 확인되었으나 naringenin과 kaempferol에서는 교배친 보다 높은 함량을 보이는 계통을 발견할 수 없었다. 총 플라보노이드 함량에서 kampferol은 79.7 %, quercetin은 17.4 %의 비율을 보였고 naringenin의 경우 2.8 %로 거의 대부분의 계통에서 가장 낮은 함량을 보이고 22개 계통에서는 전혀 발견되지 않았다. 또한 성분간 상관관계를 분석한 결과 quercetin과 kaempferol의 함량간에 유의한 상관관계가 있음을 확인할 수 있었다. 본 실험의 결과는 향후 플라보노이드 생합성 기작 관련 유전자 탐색을 위한 자료로 활용할 예정이다. 또한 고정 계통을 대상으로 다양하고 광범위한 형질 조사를 실시하여 배추의 품종 개발이 가능할 것으로 기대된다.

• 농업과학연구
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• 제44권4호
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• pp.531-540
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• 2017

A study was conducted to compare growth performance of six female commercial Korean native chicken (KNC) crossbreeds from hatching to twelve weeks of age. Three hundred and twelve, 1-day-old female commercial KNC were used within 1 paternal line and 6 maternal lines. The chickens were allocated to 24 battery cages to give 4 replicates per strain with 13 chickens per cage. The chickens were reared under continuous lighting (24 h) and water was available at all times. Ad-libitum feeding was practiced throughout the experimental period. Among the six different strains, 2A had the greatest bodyweight (BW) at 42 days after hatching (p < 0.05). No BW difference between six crossbreed strains (p > 0.05) was found thereafter. Crossbreed 1A had the higher average daily gain (ADG) than crossbreed 2A and 3A chickens (p < 0.05), whereas crossbreed 4A, 5A, and 6A had similar ADGs to that of crossbreed 1A (p > 0.05) at 84 days after hatching. Furthermore, crossbreed 4A had a great average daily feed intake (ADFI) from hatching to 84 days (p < 0.05). Nonetheless, there was no difference in the feed conversion ratio (FCR) and uniformity between six crossbreed strains for the experimental period (p > 0.05). Despite that 1A, 4A, and 6A had the higher viability (p < 0.05) than crossbreed 2A and 5A, they had a similar viability than crossbreed 3A (p > 0.05). With this in mind, crossbreed 2A had greater BW, ADG, and FCR than other chicken crossbreeds from hatching to 84 days, although they had a lower viability than others.

• 한국환경보건학회지
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• 제42권6호
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• pp.396-408
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• 2016

Objectives: The purposes of this study were to determine the relationship between the prevalence of allergic diseases and urinary cadmium concentration among children from six to 12 years of age, and to evaluate the association between cadmium in urine and other immunologically related factors in two elementary schools with different environmental conditions. Methods: Data on physician-diagnosed prevalence over the past 12 months and potential risk factors for allergic disease were collected from survey results drawn from 236 children living in different regions (central urban vs. suburban) from April to July in 2014 by an ISAAC questionnaire. We analyzed cytokine levels in serum through enzyme-linked immunosorbent assay and urinary cadmium concentration by use of inductively coupled plasma mass spectrometry. Results: Concentrations of urinary cadmium in suburban and central urban children were $0.11{\pm}0.11ug/L$, $0.14{\pm}0.17ug/L$, respectively (p>0.05). Results from the analysis of the t-test and chi-square test showed that urinary cadmium levels were positively associated with environmental factors (paternal smoking status, passive smoking, traffic volume and experiences of outdoor odors) and individual genetic factors (parental allergic disease). Cadmium concentrations in urine were also positively associated with the prevalence of allergic disease and immune-related cytokines (eosinophils, IgE, IL-5, IL-33, IL-17). Conclusion: This study suggests that genetic factors (parental history of allergic diseases), environmental factors, and regional status (suburban and central urban) should be considered as probable factors increasing the concentration of urinary cadmium, which has the potential to influence the allergic disease prevalence of school-age children.

• 동의생리병리학회지
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• 제16권2호
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• pp.397-402
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• 2002

Clinical studies were done treated with aroma therapy in 22cases of patients for suffering allergic rhinitis. who were treated at Dept of Oriental respiratory internal medicine in the Hospital of Taejeon University from January 2001 to May 2001. The results were as follows. 1. In distribution of sex & age. 45.5% of them were man, and 54.5% of them were woman. Of those who were attached, 2. In distribution of occupation, 59% of them were student, 18% of them were housewife, 18% of them were white color, 9% Of them were blue color. 3. In distribution of the period of the clinical history, under six months was 32%, under 1 year was 9%, under 3 years was 27%, over 3 years was 32%. 4. In distribution of the seasonal contributing factor, most of them were perenial allergic rhinitis, others generally started in spring or winter. 5. In distribution of past history, 59% of them are none, 18% of them are asthma, 18% of them are atopic dermatitis, 5% of them are allergic dermatitis. 6. In distribution of family history, 46% of them are none, 27% of them are sibling line, 18% of them are paternal line, 9% of them are maternal line. 7. In distribution of symptom, the ratio of sneezing was 90%, nasal discharge was 81 %, nasal obstruction was 46%, pruritus was 41 %, headache was 31%, asthma was 22%, digestion disorder was 18%, tears was 9%, nosebreeding was 5%. 8. In distribution of diagnosis in descending order, wind-cold evil was 5%, spleen-lung energy asthnia was 31 %, lung-kidney asthnia was 64%. 9. In distribution of Herb medicine treatment, Tonggyu-tang was the most, the second was hyunggaiyunyo-tang. 10 In emotional change of patients aroma-therapy, only 14% of them felt good. 11. The total remedial value of the 22 patients was revealed 32.9%. 12. In proportion to grow older, the remedial value were decreased. 13. In proportion to the period of the clinical history was longer, the remedial value were decreased.

• 여성건강간호학회지
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• 제1권2호
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• pp.174-199
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• 1995

This study examined the antenatal care known as Taekyo. The sample consisted of 795 women and 564 men who had be seen in the antenatal care unit, delivery room, or postpartal care unit of general hospitals in Korea between March 20 and April 22, 1995. Data were collected using the "Taekyo questionnaire for childbearing women" and "Taekyo questionnaire for husbands of childbearing women" developed by researchers. Data were self-reported. Data were analyzed using the SPSS PC+ program, and descriptive statistics, T-test, ANOVA, Pearson correlation coefficient were applied. The results of this study were as follows : 1. Encouragement to practice Taekyo was at a relatively high level. Items on Taekyo encouraging behaviors had a mean score of 3.51(33 items 5 point scale). According to the five categories of Taekyo encouraging behaviors, subjects indicated they practice "food intake(mean score 4.02)". "praying(mean score 3.78)", "cumulative virtuous deeds (mean score 3.58)". "mind and body management(mean score 3.47)", "maternal fetal interaction(mean score 3.15)". 2. The childbearing women's practices related to forbidden behaviors by Taekyo were relatively high. Item on forbidden behaviors by Taekyo had a mean score 3.71(43 items 5 point scale). According to the five categories of forbidden behaviors by Taekyo, subjects indicated they practiced "voluntary abstention of drugs(mean score 4.78)," avoiding behaviors(mean score 4.78)," avoiding behaviors(mean score 3.77), "good behaviors(mean score 3.71)", "taboo on fetal death tendency (mean score 3.53)", "taboo on certain intake(mean score 3.47)". 3. The practice score of husbands for childbearing women which related to Taekyo behaviors were relatively high. Item on Taekyo behaviors had a mean score 3.59(33 items 5 point scale). According to the six categories of Taekyo behavior, subjects indicated they practiced "mind and body management(mean score 3.94)", "praying(mean score 3.80)", "support of women’s practice related to Taekyo(mean score 3.66)", "good behaviors(mean score 3.58)", "continency(mean score 3.33)", "paternal fetal interaction (mean score 3.19)". 4. On the childbearing women's perception of Taekyo, most of the subjects(88%) had confidence in the positive effects of Taekyo on Child development. The result showed that the childbearing couples practiced Taekyo behavior relatively often and sincerely and most of the childbearing women had a positive perception of Taekyo's effect on prenatal child development.

• 한국양식학회지
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• 제19권4호
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• pp.281-287
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• 2006

우리나라 주요 담수어종인 미꾸라지(Misgurnus mizolepis)를 어류 염색체 조작 모델로 개발하기 위한 연구의 일환으로 동일 종내 웅성발생성 이배체 유도 조건을 개발하고 유도된 웅성발생성 이배체의 생존능력을 평가하였다. 자외선(UV; $10,800\;ergs/mm^2$)을 이용하여 난자의 유전물질을 불활성화시키고 인공수정 28분 후(제1난할 중기)에 온도처리(고온 자극 $40.5\;^{\circ}C$ 120초 후 저온 자극 $1\;^{\circ}C$ 45분)를 통해 제1난할을 억제하였다. 본 온도처리를 통해 생존력 있는 웅성발생성 이배체들이 유도되었고(평균 부화율, 26.9%), 부화 후 1주일째를 기준으로 평균 웅성발생성 이배체 수율은 최초 처리난의 7%내외로 나타났다. 그러나 웅성발생성 이배체 유도 효율은 cytoplasmic donor의 질에 크게 영향을 받음이 관찰되어 사용한 암컷 친어별로 큰 편차가 관찰되었다. Flow cytometry 분석에 의해 생존력 있는 웅성발생성 개체들의 이배체 복원이 확인되었으며 아울러 형질전환 표지를 이용하여 동형접합성 개체 유도의 가능성이 확인되었다. 웅성발생성 이배체들은 일반대조군 이배체에 비해 낮은 생존능력을 보였으며 특히 부화 후 1개월까지 유의적으로 낮은 생존율을 나타내었다. 그러나 후기 생존능력은 일반 이배체군과 유사하였다.

• 한국가금학회지
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• 제41권3호
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• pp.217-225
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• 2014

텔로미어는 진핵 세포의 염색체 말단으로, 직렬 반복 DNA 염기 서열과 shelterin 단백질 복합체로 구성되어 있다. 텔로미어의 기능은 염색체를 보호하는 것으로 체세포의 텔로미어 길이는 세포 분열시 DNA 복제 결실로 인해 연령이 증가함에 따라 감소하는 경향이 있다. 그러나 유전적, 후생유전학적 및 환경적 수준에서 여러 가지 요인이 텔로미어 길이에 영향을 미친다. 따라서 본 연구에서는 닭의 텔로미어 길이의 유전력을 추정하고, 이들의 유전전이 양상을 살펴보고자 하였다. 텔로미어 길이는 백혈구를 이용하여 양적 형광접합보인법(Q-FISH)과 양적 중합효소 연쇄반응법(qRT-PCR)으로 분석하였다. 분석 결과, 텔로미어 길이의 유전력은 자손과 부모 회귀 분석에 의해 출생 시 0.9로 추정되었고, 10 주령 및 30주령 때 부 분산 분석에 의해 0.03과 0.04로 추정되었다. 부와 자손 간(r=0.348) 및 모와 자손 간(r=0.380) 텔로미어 길이는 모두 유의한 정의 상관 관계를 보였다. 따라서 닭 텔로미어의 유전 전이 양상은 부모 양쪽 모두로부터 비슷하게 자식에 영향을 미치는 것으로 판단된다. 더불어 암수 자손에 미치는 영향 또한 유사한 것으로 나타났다. 이러한 결과는 부모의 텔로미어 길이의 각인이 성염색체의 유전자가 아닌 상염색체의 유전자에 의해 조절되는 것을 의미한다. 또한, 산모 연령에 따른 출생 자손의 텔로미어 길이는 차이가 없는 것으로 나타났다. 따라서 모체의 연령이 출생 자손의 텔로미어 길이에 영향을 미치지 않는데, 이는 수정란의 초기 배아 단계에서 세포적 reprogramming이 이루어지기 때문으로 사료된다.