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Intraspecific Androgenesis in Mud Loach (Misgurnus mizolepis): II. Diploid Restoration and Viability Assessment  

Nam, Yoon-Kwon (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University)
Bang, In-Chul (Department of Marine Biotechnology, Soonchunhyang University)
Noh, Choong-Hwan (Marine Resources and Research Department, Korea Ocean Research and Development Institute)
Cho, Young-Sun (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University)
Kim, Dong-Soo (Department of Aquaculture & Institute of Marine Living Modified Organisms (iMLMO), Pukyong National University)
Publication Information
Journal of Aquaculture / v.19, no.4, 2006 , pp. 281-287 More about this Journal
Abstract
Intraspecific diploid androgenesis was achieved in mud loach (Misgurnus mizolepis) by the inhibition of the first mitotic division using combined thermal treatment. A combined thermal treatment (heat shock at $40.5\;^{\circ}C$ for 120 sec followed by cold treatment at $1\;^{\circ}C$ for 45 min) applied to the 1st metaphase of cell division (28 min post insemination at $25\;^{\circ}C$) successfully recovered viable androgenetic diploidy. Mean hatching success of the androgenetic diploid group was 29.6%, and the average yield out of total eggs taken was about 7% assessed at 1 week of age. However, relatively large variations in the yield of diploid androgenesis were observed among different egg batches used as cytoplasmic donors. Successful diploidization was confirmed by flow cytometric analysis, and parthenogenic reproduction in a paternal exclusive manner was verified with transgene dosage. Significant mortality was found in most androgenetic groups especially from hatch to 1 month of age, although such mortality was stabilized later.
Keywords
Diploid androgenesis; Mud loach; Parthenogenic reproduction; Viability;
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1 Arai, K., 2001. Genetic improvement of aquaculture finfish species by chromosome manipulation techniques in Japan. Aquaculture, 197, 205-228   DOI   ScienceOn
2 Babiak, I., S. Dobosz, K. Goryczko, H. Kuzminski, P. Brzuzan and S. Ciesielski, 2002a. Androgenesis in rainbow trout using cryopreserved spermatozoa: the effect of processing and biological factors. Theriogenology, 57, 1229-1249   DOI   ScienceOn
3 David, C. J. and T. J. Pandian, 2006. Cadaveric sperm induces intergeneric androgenesis in the fish, Hemigrammus caudovittatus. Theriogenology, 65, 1048-1070   DOI   ScienceOn
4 Nam, Y. K., C. H. Noh and D. S. Kim, 1999. Transmission and expression of an integrated reporter construct in three generations of transgenic mud loach (Misgurnus mizolepis). Aquaculture, 172, 229-245   DOI   ScienceOn
5 Nam, Y. K., C. H. Noh and D. S. Kim, 2006. Intraspecies androgenesis in mud loach (Misgurnus mizolepis): I. Optimization of the egg inactivation and haploid androgenesis using transgene marker. J. Aquacult., 19 (in press)   과학기술학회마을
6 Pandian, T.J. and R. Koteeswaran, 1998. Ploidy induction and sex control in fish. Hydrobiologia, 384, 167-243   DOI   ScienceOn
7 Thorgaard, G. H., 1986. Ploidy manipulation and performance. Aquaculture, 57, 57-64   DOI   ScienceOn
8 Bongers, A. B. J., J. B. Abraca, Z. Doulabi, E. H. Eding, J. Komen and C. J. J. Richter, 1995. Maternal influence on development of androgenetic clones of common carp, Cyprinus carpio L. Aquaculture, 137, 139-147   DOI   ScienceOn
9 Kim, D. S., J.-Y. Jo, and T.-Y. Lee, 1994. Induction of triploidy in mud loach (Misgurnus mizolepis) and its effect on gonad development and growth. Aquaculture, 120, 263- 270   DOI   ScienceOn
10 Nam, Y. K., G. C. Choi and D. S. Kim, 2004. An efficient method for blocking the 1st mitotic cleavage of fish zygote using combined thermal treatment, exemplified by mud loach (Misgurnus mizolepis). Theriogenology, 61, 933-945   DOI   ScienceOn
11 Kirankumar, S. and T. J. Pandian, 2003. Production of androgenetic tiger barb Puntius tetrazona. Aquaculture, 228, 37-51   DOI   ScienceOn
12 Maremgpmo, M. G. and Y. Onoue, 1998. Ultraviolet-induced androgenesis in nile tilapia, Oreochromis niloticus (L.), and hybrid nile x blue tilapia, O. aureus (Steindachner). Aquaculture Res., 29, 359-366
13 Nam, Y. K., Y. S. Cho, H. J. Cho and D. S. Kim, 2002. Accelerated growth performance and stable germ-line transmission in androgenetically derived homozygous transgenic mud loach, Misgurnus mizolepis. Aquaculture, 209, 257-270   DOI   ScienceOn
14 Paschos, I., L. Natsis, C. Nathanailides, I. Kagalou and E. Kolettas, 2001. Induction of gynogenesis and androgenesis in goldfish Carassius auratus (var. oranda). Reprod. Dom. Anim., 36, 195-198   DOI
15 Bongers, A. B. J., E. P. C. in't Veld, K. Avo-Hashema, I. M. Bremmer, E. H. Eding, J. Komen and C. J. J. Richter, 1994. Androgenesis in common carp (Cyprinus carpio L.) using UV irradiation in a synthetic ovarian fluid and heat shocks. Aquaculture, 122, 119-132   DOI   ScienceOn
16 Bercsenyi, M., I. Magyary, B. Urbanyi, L. Orban and L. Horvath, 1998. Hatching out goldfish from common carp eggs: interspecific androgenesis between two cyprinid species. Genome, 41, 573-579   DOI
17 Kirankumar S. and T. J. Pandian, 2004. Interspecific androgenetic restoration of rosy barb using cadaveric sperm. Genome, 47, 66-73   DOI   ScienceOn
18 Babiak, I., S. Dobosz, H. Kuzminski, K. Goryczko, S. Ciesielski, P. Brzuzan, B. Urbanyi, A. Horvath, F. Lahnsteiner and J. Piironen, 2002b. Failure of interspecies androgenesis in salmonids. J. Fish. Biol., 61, 432-447   DOI
19 Corley-Smith, G. E., C. J. Lim and B. P. Brandhorst, 1996. Production of androgenetic zebrafish (Danio rerio). Genetics, 142, 1265-1276
20 Zhang, H., B., Robison, G. H. Thorgaard and S. S. Ristow, 2000. Cloning, mapping and genomic organization of a fish C-type lectin gene from homozygous clones of rainbow trout (Oncorhynchus mykiss). Biochim. Biophys. Acta, 1494, 14-22   DOI   ScienceOn
21 Tanck, M. W. A. P. Palstra, M. van der Weerd, C. P. Leffering, J. J. van der Poel, H. Bovenhuis and J. Komen, 2001. Segregation of microsatellite alleles and residual heterozygosity at single loci in homozygous androgenetic common carp (Cyprinus carpio L.). Genome, 44, 743-751
22 Nam, Y. K., Y. S. Cho, Y. J. Chang, J.-Y. Jo and D. S. Kim, 2000. Generation of transgenic homozygous line carrying the CAT gene in mud loach Misgurnus mizolepis. Fish. Sci., 66, 58-62   DOI   ScienceOn
23 Lin, F. and K. Dabrowski, 1998. Androgenesis and homozygous gynogenesis in muskellunge (Esos masquinongy): evaluation using flow cytometry. Mol. Reprod. Dev., 49, 10-18   DOI   ScienceOn
24 Cho, Y. S., B. N. Choi, E.-M. Ha, K. H. Kim, S. K. Kim, D. S. Kim and Y. K. Nam, 2005. Shark, Scyliorhinus torazame metallothionein: cDNA cloning, genomic sequence and expression analysis. Mar. Biotechnol., 7, 350-362   DOI