• Title/Summary/Keyword: parent selection

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Selection of a L-Lysine-Overproducing Strain of the Red Seaweed Porphyra suborbiculata (Rhodophyta) through Mutation and Analog Enrichment

  • Luyen, Quoc-Hai;Chowdhury, Muhammad Tanvir Hossain;Choi, Jae-Suk;Kang, Ji-Young;Park, Nam-Gyu;Hong, Yong-Ki
    • Fisheries and Aquatic Sciences
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    • v.15 no.2
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    • pp.145-150
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    • 2012
  • An improved strain of the red seaweed Porphyra suborbiculata containing an increased amount of the essential amino acid L-lysine was obtained through mutation and analog enrichment. Mutagenesis using a 10% lethal dose of ultraviolet irradiation and an enrichment culture with the L-lysine analog aminoethyl-L-cysteine (AEC) was repeated to select the most productive strain using monospores of P. suborbiculata. The concentrations of AEC required to produce 50 and 100% inhibition of survival were 60 and 115 mM in the parent strain, and 72 and 135 mM in the selected AEC-resistant strain, respectively. The AEC-resistant strain, L130, produced 1.74-fold more lysine compared to its parent strain. Thus, mutagenesis with analog enrichment shows promise for selecting seaweed strains that can overproduce this essential amino acid.

Determinants Affecting Thai Merchant Marine Students' Decision in Selecting a Maritime Institute in Thailand: Nautical Science Program (태국의 해기교육기관 선택에 있어서 상선 사관 학생들의 결정에 영향을 미치는 요인: 항해 프로그램)

  • Luksanato, Sarawut
    • Journal of Navigation and Port Research
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    • v.37 no.4
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    • pp.359-366
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    • 2013
  • The objective of the research was to study the determinants affecting the decision to study nautical science program within a Thai maritime institute in preparation for working post-graduation as a ship officer on a merchant ship. The samples are classified by institute, academic year, cumulative score level, domicile, and parent's monthly income. The total sample of study was 386 Thai merchant marine students. The data collection method was a one to five rating scale questionnaire. The statistical methods applied in analyzing the data were percentage, mean, standard deviation, t-test, one way analysis of variance and a Sheffe's test. The study shows seven factors that influenced the decision in descending order; expectations, tuition and scholarships, selection system, quality of the institute, background and private capability, generality of the institute and external influences on the decision. The decision to select an institute was classified by institute and revealed that different institutes had distinct determinants that led to the decision. The students were from The Merchant Marine Training Center and from The International Maritime College, Kasetsart University were differences. There were no dissimilarity between academic year, cumulative score level, domicile, and parent's monthly income.

Hypothetical Study of Student's Academic Performance influenced by Parent's Educational and Financial Status

  • Zohra Khatoon;Hussain Saleem;Ameer Ali Buriro;Uzma Murad Panhwar;Samina Saleem
    • International Journal of Computer Science & Network Security
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    • v.24 no.7
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    • pp.79-86
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    • 2024
  • This research is conducted in order to analyze the student's academic performance at the secondary school level in Sindh province of Pakistan. The study was focused on the students who have passed the Matriculation level recently. The sample of size equal to 1097 secondary level students was randomly selected in a way that from each college roughly up to 150 students took part in the survey. The sample selection was further divided on gender (Male=448, Female=649) and Locale (Urban=456, Rural=641). A survey questionnaire was circulated for data collection. The statistical analysis based on Pearson's Chi-Square and Correlation models were carried out using the collected data. The conclusion was drawn from results that strongly revealed that the student's academic achievement at high school level was highly associated to their parent's educational level and socio-economic background. Therefore, it is strongly recommended that the financial condition of the population must be enhanced by taking suitable measures. The affected students should be awarded adequate financial assistance or scholarships to face such hardships of their learning career. Free laptops, learning materials, books, and stationery should also be provided.

Detection of Mendelian and Parent-of-origin Quantitative Trait Loci for Meat Quality in a Cross between Korean Native Pig and Landrace

  • Choi, B.H.;Lee, Y.M.;Alam, M.;Lee, J.H.;Kim, T.H.;Kim, K.S.;Kim, J.J.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.12
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    • pp.1644-1650
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    • 2011
  • This study was conducted to detect quantitative trait loci (QTL) affecting meat quality in an $F_2$ reference population of Korean native pig and Landrace crossbreds. The three-generation mapping population was generated with 411 progeny from 38 $F_2$ full-sib families, and 133 genetic markers were used to produce a sex-average map of the 17 autosomes. The data set was analyzed using least squares Mendelian and parent-of-origin interval-mapping models. Lack-of-fit tests between models were used to characterize the QTL for mode of gene expressions. A total of 10 (32) QTL were detected at the 5% genome (chromosome)-wise level for the analyzed traits. Of the 42 QTL detected, 13 QTL were classified as Mendelian, 10 as paternal, 14 as maternal, and 5 as partial expressed QTL, respectively. Among the QTL detected at 5% genome-wise level, four QTL had Mendelian mode of inheritance on SSCs 5, 10, 12, and 13 for cooking loss, drip loss, crude lipid and crude protein, respectively; two QTL maternal inheritance for pH at 24-h and shear force on SSC11; three QTL paternal inheritance for CIE b and Hunter b on SSC9 and for cooking loss on SSC15; and one QTL partial expression for crude ash on SSC13, respectively. Most of the Mendelian QTL (9 of 13) had a dominant mode of gene action, suggesting potential utilization of heterosis for genetic improvement of meat quality within the cross population via marker-assisted selection.

Development of Fusant Degrading Aniline and 4-chlorobiphenyl by Spheroplast Fusion between Pseudomonas sp. and Flavimonas oryzihabitans (Flavimonas oryzihabitans와 Pseudomonas sp.간 원형질체 융합에 의한 Aniline과 4-chlorobiphenyl 분해균주 개발)

  • 박형수;박용근;김무훈;고범준;조미영;김치경
    • Korean Journal of Microbiology
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    • v.36 no.4
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    • pp.259-266
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    • 2000
  • Spheroplast cell fusions were performed with Flavimonas oryzihabitans degrading aniline and Pseudomonas sp. degrading 4-chlorobiphenyl to develope the new fusant degrading aniline and 4-CBP and its characters were investigated. F. oryzihabitans was induced to antibiotic marker ($Cm^r$ by NTG treatment for the fusants selection. The results of spheroplast formation and regeneration frequencies of the strains treated with lysozyme-EDTA were 99% and 5.0~6.6%, respectively. Fusion products were treated with 40% (v/v) PEG 6000 and fusion frequency was $3.16{\times}10^{-4} $. The DNA content of fusant, F22 was approximately 2-fold compared with parents. The fusant was stable, and showed the mixed biochemical characteristics of the parent strains. F22 was similar to parent for cell growth pattern and degrading capacity on 5 mM aniline but cell growth rate of F22 was 1.5-fold higher than that of the parent on 10mM aniline. However 4-CBP degrading ability of F22 was slightly lower than that of parental strain.

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Comparison on genomic prediction using pedigree BLUP and single step GBLUP through the Hanwoo full-sib family

  • Eun-Ho Kim;Ho-Chan Kang;Cheol-Hyun Myung;Ji-Yeong Kim;Du-Won Sun;Doo-Ho Lee;Seung-Hwan Lee;Hyun-Tae Lim
    • Animal Bioscience
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    • v.36 no.9
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    • pp.1327-1335
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    • 2023
  • Objective: When evaluating individuals with the same parent and no phenotype by pedigree best linear unbiased prediction (BLUP), it is difficult to explain carcass grade difference and select individuals because they have the same value in pedigree BLUP (PBLUP). However, single step GBLUP (ssGBLUP), which can estimate the breeding value suitable for the individual by adding genotype, is more accurate than the existing method. Methods: The breeding value and accuracy were estimated with pedigree BLUP and ssGBLUP using pedigree and genotype of 408 Hanwoo cattle from 16 families with the same parent among siblings produced by fertilized egg transplantation. A total of 14,225 Hanwoo cattle with pedigree, genotype and phenotype were used as the reference population. PBLUP obtained estimated breeding value (EBV) using the pedigree of the test and reference populations, and ssGBLUP obtained genomic EBV (GEBV) after constructing and H-matrix by integrating the pedigree and genotype of the test and reference populations. Results: For all traits, the accuracy of GEBV using ssGBLUP is 0.18 to 0.20 higher than the accuracy of EBV obtained with PBLUP. Comparison of EBV and GEBV of individuals without phenotype, since the value of EBV is estimated based on expected values of alleles passed down from common ancestors. It does not take Mendelian sampling into consideration, so the EBV of all individuals within the same family is estimated to be the same value. However, GEBV makes estimating true kinship coefficient based on different genotypes of individuals possible, so GEBV that corresponds to each individual is estimated rather than a uniform GEBV for each individual. Conclusion: Since Hanwoo cows bred through embryo transfer have a high possibility of having the same parent, if ssGBLUP after adding genotype is used, estimating true kinship coefficient corresponding to each individual becomes possible, allowing for more accurate estimation of breeding value.

Effects of Parent-Child Interaction Therapy on Parenting Skill and Children's Problem Behaviors: Meta-Analysis (부모-아동 상호작용치료가 양육기술과 아동의 문제행동에 미치는 영향: 메타분석)

  • Park, Min-Kyoung;Bak, Ah-Ream;Yoo, Doo-Han
    • The Journal of Korean Academy of Sensory Integration
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    • v.16 no.2
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    • pp.50-63
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    • 2018
  • Objective : The purpose of this study is to examine the effects of parent - child interaction therapy on parenting skills and children's problem behaviors by meta - analysis and to provide a basis for intervention methods. Methods : This study was conducted from January 2007 to March 2018 and the study was conducted using the parent-child interaction program for children with disabilities. PubMed and CINAHL, EBSCOhost, Science Direct were used to search for studies, and reference searching was also conducted as a secondary search option. A total of 7 studies was selected to meet the selection criteria, and the final 7 studies were assessed by using the Jadad scale. Statistical tests were performed using the R-meta-analysis program. Results : The effect size of parenting skill was large and the size of the effect of parenting stress was moderate. The children's problem behaviors also showed moderate effect sizes and all p-values were statistically significant (p < .0001). Conclusion : This study examined the effects of parent-child interaction therapy on parenting skills, parenting stress, and children's problem behaviors. Future research is needed to establish the basis for intervention for family involvement in pediatric occupational therapy.

A New Medium-maturity Rice Cultivar Developed from an Interspecific Cross, "Hwaweon 1" (종간교잡 유래 중생 고품질 벼 품종 "화원 1호")

  • Ahn, Sang-Nag;Ju, Hong-Guang;Kim, Dong-Min;Oh, Chang-Sik;Kang, Ju-Won;Jin, Feng-Xue;Oh, Ji-Min
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.338-341
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    • 2009
  • "Hwaweon 1" was developed from an interspecific cross between Hwaseongbyeo and a wild species, Oryza rufipogon L. (IRGC 105491) based on marker-aided selection. The recurrent parent "Hwaseongbyeo" is a high grain quality cultivar with medium-maturity. Hwaweon 1 is nearly isogenic to Hwaseongbyeo except a small O. rufipogon introgression on chromosome 8 harboring the gene for grain weight. The preliminary and replicated yield trial was conducted at Chungnam National University in 2004, 2005 and 2006. The local adaptability test was carried out by the National Seed Management Office (NSMO) in 2007 and 2008. This cultivar was registered to NSMO with a cultivar designated as "Hwaweon 1". This cultivar averaged 89 cm in culm length and has a medium growth duration. This cultivar is resistant to stripe virus as the recurrent parent, Hwaseongbyeo. Milled rice of "Hwaweon 1" is translucent and the grain quality traits are comparable to those of the recurrent parent. The yield potential of "Hwaweon 1" in grain is about 7.43 MT/ha at the ordinary fertilizer level about 10% higher than that of Hwaseongbyeo mainly due to increase in grain weight.

Breeding of "Wongyo 3111", Intermediate Strawberry Parent Line with High Sugar Content and Large-sized Fruit (고당도 대과성 특성을 지닌 중간모본용 딸기 "원교 3111호" 육성)

  • Rho, Il Rae;Cho, Yong Seop;Cheong, Jae Wan;Jeong, Ho Jeong
    • Korean Journal of Breeding Science
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    • v.41 no.3
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    • pp.319-323
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    • 2009
  • Strawberry is an out-crossed crop that is relatively sensitive to inbreeding, and as a result, most of breeding programmes in strawberry have been based on selection breeding where elite parents are selected from intercrossing. Also, pedigree breeding has been successfully employed in a number of instances to concentrate genes of interest. Therefore, in order to obtain inbred line having the genotype of excellent combining ability by selfing and incrossing to improve breeding efficiency of strawberry, elite varieties were self-pollinated. From the selfing line of "Benihoppe" cultivar, we selected "Wongyo 3111" having excellent horticultural traits such as plant vigor, sugar content and fruit hardness among these progenies. "Wongyo 3111" shows erect plant type, vigorous growth habit, early flower bud differentiation with 16~17 flowers per cluster. Fruits of "Wongyo 3111" are conical type having a bright red skin color, and 17.2 g in an average weight. Also that have a relatively more high sugar/acid ratio and firmness than check cultivars as sugar content of 11.3oBx, acidity of 0.50% and firmness of 21.7 g/$mm^2$. But although yield of "Wongyo 3111" is not significantly different from check cultivars, its marketable yield is remarkably lower than that of check cultivars because of poor fruit-setting under low temperature. Disease and pest reactions of "Wongyo 3111" are more sensitive to powery mildew, anthracnose and spotted spider mites than check cultivars. Therefore, "Wongyo 3111" is suitable for intermediate parent line better than as a cultivation cultivar.

A New High-yielding Rice Variety 'Hwaweon 7' with Lodging Tolerance (벼 도복저항성 다수성 신품종 '화원7호')

  • Kim, Dong-Min;Kang, Ju-Won;Yun, Yeo-Tae;Lee, Hyun-Sook;Park, In-Kyu;Ahn, Sang-Nag
    • Korean Journal of Breeding Science
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    • v.49 no.3
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    • pp.250-255
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    • 2017
  • 'Hwaweon 7' was developed from a cross between the African upland cultivar, 'Moroberekan' and 'Ilpumbyeo' based on marker-aided backcross selection. The recurrent parent, Ilpumbyeo is a high grain quality cultivar with medium to late maturity. Hwaweon 7 is nearly isogenic to Ilpumbyeo except a small Moroberekan introgressed segment on chromosome 6 harboring genes related with spikelets per panicle and internode diameter. The preliminary and replicated yield trial was conducted at Chungnam National University and Chungcheongnamdo Agricultural Research & Extension (CARES), Yesan in 2010 and 2011. The local adaptability test was carried out by the National Seed Management Office (NSMO) in 2012 and 2013. This cultivar was registered to NSMO with a cultivar designated as "Hwaweon7". This cultivar averaged 80cm in culm length and has a medium growth duration. Milled rice of "Hwaweon7" is translucent and the grain quality traits are comparable to those of the recurrent parent. The average yield potential of Hwaweon 7 in grain was about 6.48 MT/ha at the ordinary fertilizer level for two years about 5.1% higher than that of Ilpumbyeo due to increase in spikelets per panicle. This variety is tolerant to lodging and the tolerance is due to the APO1 gene controlling the base internode diameter on chromosome 6 introgressed from the donor parent, Moroberekan. The Apo1 gene would be useful in enhancing resistance to lodging in rice breeding program.