• Journal of Microbiology and Biotechnology
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• v.30 no.5
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• pp.762-769
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• 2020

Vitamin K2 (menaquinone) is an essential vitamin existing in the daily diet, and menaquinone-7 (MK-7) is an important form of it. In a recent work, we engineered the synthesis modules of MK-7 in Bacillus subtilis, and the strain BS20 could produce 360 mg/l MK-7 in shake flasks, while the methylerythritol phosphate (MEP) pathway, which provides the precursor isopentenyl diphosphate for MK-7 synthesis, was not engineered. In this study, we overexpressed five genes of the MEP pathway in BS20 and finally obtained a strain (BS20DFHG) with MK-7 titer of 415 mg/l in shake flasks. Next, we optimized the fermentation process parameters (initial pH, temperature and aeration) in an 8-unit parallel bioreactor system consisting of 300-ml glass vessels. Based on this, we scaled up the MK-7 production by the strain BS20DFHG in a 50-l bioreactor, and the highest MK-7 titer reached 242 mg/l. Here, we show that the engineered strain BS20DFHG may be used for the industrial production of MK-7 in the future.

• Journal of the Korean Society for information Management
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• v.25 no.3
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• pp.143-158
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• 2008

This study was conducted under the premise of providing the model for the construction of the library and the digital library in PUST, the joint construction from South and North Korea. First, we determined the problems in the construction of digital libraries as well as possible issues that may rise from the construction of the digital library in PUST. The results of the research showed that there were imminent problems from the operation of a digital library with the inadequate progress in the Held of copyright. In addition, the difference in the language system and the knowledge foundations of the two countries will produce problems in the homepage access, database construction, and information retrieval. In order to overcome these predictable problems, this research proposes the following: (1) parallel operation of both digital and high-drive libraries; (2) duplexing the homepage through the application of unicode concerning the digital library; (3) development and application of converted letter codes through the establishment of NCHAR data type; and (4) construction of an authority database.

• Journal of Biosystems Engineering
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• v.6 no.1
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• pp.1-14
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• 1981

A laboratory model test was carried out with a newly designed model to figure out the vibration characteristics of the vibratory tillage tool according to the method of forced vibration, i.e., horizontally and vertically forced vibrations. The results are summarized as follows: 1. The reduction ratios of the draft force of the vibratory blade were 14.2-42.6% for the case where the vibration was forced parallel to the travelling direction of the blade, and 15-54.5% for the vertically forced vibration. And it was thought that the method of vertically forced vibration was preferable to the reduction of the draft force. 2. The ratio of the draft force of a vibratory blade to that of a static one could be represented as a function of V/At. It was found to be possible to reduce the draft force by taking a lower value of (V/Af) and this meant that the effictiveness of tillage practice using the vibratory system would be limited. 3. The torque to the main rotating shaft to vibrate the model blade increased frequency and amplitude. This tendency varied according to the physical properties of tested soil. In case of horizontally forced vibration, the torque was 8~34% less than in case of vertically forced vibration. 4. With the increase of frequency, the total power requirement increased linearly, and also the portion of oscillating power requirement in the total power tended to increase. The magnitude of the total power requirement was 1.4-13 times greater than that of a static one for the case of horizontal vibration, and 1.5-15 times greater for the case of vertical vibration. It was thought that the horizontal vibration of the blade was preferable to the vertical vibration in view of the power requirement. 5. A linearity was found between the amplitude of moment oscillogram and magnitude of oscillating acceleration. Only positive values of moment occurred when the blade was forced to vibrate vertically, but negative values occurred in rarity in the case of amplitude A3 when the blade was forced to vibrate horizontally.

• Journal of the Korean Society for Aeronautical & Space Sciences
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• v.31 no.5
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• pp.18-24
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• 2003

The design cycle associated with large engineering systems requires an initial decomposition of the complex system into design processes which are coupled through the transference of output data. Some of these design processes may be grouped into iterative sybcycles. Previous researches predifined the numbers of design processes in groups, but these group sizes should be determined optimally to balance the computing time of each groups. This paper proposes adaptive decomposition method, which determines the group sizes and the order of processes simultaneously to raise design efficiency by expanding the chromosome of the genetic algorithm. Finally, two sample cases are presented to show the effects of optimizing the sequence of processes with the adaptive decomposition method.

• Proceedings of the KOSOMBE Conference
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• v.1997 no.11
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• pp.365-368
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• 1997

We are developing a small gamma camera or imaging malignant breast tumors. The small scintillation camera system consists of NaI(Tl) crystal ($60\;{\times}\;60\;{\times}\;6\;mm^3$) coupled to position sensitive photomultiplier tube (PSPMT), nuclear instrument module (NIM), analog to digital converter (ADC), and personal computer. High quality flood source image and hole mask image were obtained using the gamma camera developed in this study. Breast phantom containing $2{\sim}7\;mm$ diameter spheres was successfully imaged with parallel hole collimator. The obtained image displayed accurate activity distribution over the imaging field of view. Linearity and uniformity correction algorithms are being developed. It is believed that the developed small gamma camera could be useful or detection of malignant breast cancer.

• Tunnel and Underground Space
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• v.27 no.6
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• pp.357-365
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• 2017

The mine subsidence prevention technology has been promoted based on the field test for design, construction, automation measurement and monitoring optimized for investigation, design and mine-filling efficiency customized in Korean mining environment. Based on the R&D roadmap ('07~'16) of the 1st and 2nd stage, mine reclamation technology development has been focused on developing method of evaluating subsidence stability, development of filling material and optimum filling technology, and development of measuring instrument. In the future, in order to systematic management for the subsidence risk areas, we intend to enhance technological capabilities and strengthen the technological infrastructure for business promotion in parallel with the discovery and introduction of new technology to prevent subsidence in the 4th Industrial Revolution era.

• Journal of Plant Biotechnology
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• v.37 no.2
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• pp.153-165
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• 2010

Brassica rapa is considered an ideal candidate to act as a reference species for Brassica genomic studies. Among the three basic Brassica species, B. rapa (AA genome) has the smallest genome (529 Mbp), compared to B. nigra (BB genome, 632 Mbp) and B. oleracea (CC genome, 696 Mbp). There is also a large collection of available cultivars of B. rapa, as well as a broad array of B. rapa genomic resources available. Under international consensus, various genomic studies on B. rapa have been conducted, including the construction of a physical map based on 22.5X genome coverage, end sequencing of 146,000 BACs, sequencing of >150,000 expressed sequence tags, and successful phase 2 shotgun sequencing of 589 euchromatic region-tiling BACs based on comparative positioning with the Arabidopsis genome. These sequenced BACs mapped onto the B. rapa genome provide beginning points for genome sequencing of each chromosome. Applying this strategy, all of the 10 chromosomes of B. rapa have been assigned to the sequencing centers in seven countries, Korea, UK, China, India, Canada, Australia, and Japan. The two longest chromosomes, A3 and A9, have been sequenced except for several gaps, by NAAS in Korea. Meanwhile a China group, including IVF and BGI, performed whole genome sequencing with Illumina system. These Sanger and NGS sequence data will be integrated to assemble a draft sequence of B. rapa. The imminent B. rapa genome sequence offers novel insights into the organization and evolution of the Brassica genome. In parallel, the transfer of knowledge from B. rapa to other Brassica crops would be expected.

• Journal of Internet Computing and Services
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• v.2 no.3
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• pp.81-92
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• 2001

The clustering environment with heterogeneous workstations provides the cost effectiveness and usability for executing applications in parallel. The load balancing is considered as a necessary feature for the clustering of heterogeneous workstations to minimize the turnaround time. Since each workstation may have different users, groups. requests for different tasks, and different processing power, the capability of each processing unit is relative to the others' unit in the clustering environment Previous works is a static approach which assign a predetermined weight for the processing capability of each workstation or a dynamic approach which executes a benchmark program to get relative processing capability of each workstation. The execution of the benchmark program, which has nothing to do with the application being executed, consumes the computation time and the overall turnaround time is delayed. In this paper, we present an efficient task distribution method and implementation of load balancing system for the clustering environment with heterogeneous workstations. Turnaround time of the methods presented in this paper is compared with the method without load balancing as well as with the method load balancing with performance evaluation program. The experimental results show that our methods outperform all the other methods that we compared.

• Journal of Internet Computing and Services
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• v.10 no.4
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• pp.199-211
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• 2009

Recent advancements in wireless networks have enabled support for mobile applications to transfer data over heterogeneous wireless paths in parallel using data striping technique [2]. Traditionally, high performance data transfer requires tuning of multiple TCP sockets, at sender's end, based on bandwidth delay product (BDP). Moreover, traditional techniques like Automatic TCP Buffer Tuning (ATBT), which balance memory and fulfill network demand, is designed for wired infrastructure assuming single flow on a single socket. Hence, in this paper we propose a buffer tuning technique at senders end designed to ensure high performance data transfer by striping data at transport layer across heterogeneous wireless paths. Our mechanism has the capability to become a resource management system for transport layer connections running on multi-homed mobile host supporting features for wireless link i.e. mobility, bandwidth fluctuations, link level losses. We show that our proposed mechanism performs better than ATBT, in efficiently utilizing memory and achieving aggregate throughput.

• Education of Primary School Mathematics
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• v.14 no.2
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• pp.117-134
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• 2011

The purpose of this research was to analyze mathematical task types to enhance creativity. Creativity is increasingly important in every field of disciplines and industries. To be excel in the 21st century, students need to have habits to think creatively in mathematics learning. The method of the research was to collect the previous research and papers concerning creativity and mathematics. To search the materials, the researcher used the search engines such as the GIL and the KISTI. The mathematical task types to enhance creativity were categorized 16 different types according to their forms and characteristics. The types of tasks include (1) requiring various strategies, (2) requiring preferences on strategies, (3) making word problems, (4) making parallel problems, (5) requiring transforming problems, (6) finding patterns and making generalization, (7) using open-ended problems, (8) asking intuition for final answers, (9) asking patterns and generalization (10) requiring role plays, (11) using literature, (12) using mathematical puzzles and games, (13) using various materials, (14) breaking patterned thinking, (15) integrating among disciplines, and (16) encouraging to change our lives. To enhance students' creativity in mathematics teaching and learning, the researcher recommended the followings: reshaping perspectives toward teaching and learning, developing and providing creativity-rich tasks, applying every day life, using open-ended tasks, using various types of tasks, having assessment ability, changing assessment system, and showing and doing creative thinking and behaviors of teachers and parents.