• Title/Summary/Keyword: paraformaldehyde

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Application of Paraformaldehyde (Neo-PPS) Fumigation to the Disinfection of the Silkworm Larvae, Bombyx mori L., for the Control of Silkworm Diseases (II). Chemical Damage of Neo-PPS to the Silkworm Larvae (Paraformaldehyde(Neo-PPS)$\circledR$훈증에 의한 잠체소독에 관한 연구 II. 약해에 관한 시험)

  • 김근영;이재창
    • Journal of Sericultural and Entomological Science
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    • v.24 no.1
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    • pp.25-27
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    • 1982
  • The use of fumigation of Neo-PPS was tested for the larval disinfection and at the same time for easy handling and effective chemicals for larval disinfection. The results obtained are as follows: 1. There was no decrease of cocoon yield in spring season due to chemical damage by application of Neo-PPS fumigation to the disinfection of the silkworm except 2,4 and 6 hours' treatment everyday and 6 hours'/ twice/instar treatment as compared with the control. 2. There was no significance in pupal percent between treatments and control in spring season except 6 hours' treatment everyday. 3. No chemical damage seemed to give rise to percentage of cocoon shell in all treatments.

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Immunohistochemical Identification of $\beta$-Endorphin in the Mouse Ovary (생쥐의 난소의 $\beta$-Endorphin에 대한 면역조직화학적 동정)

  • 조사선;이영기;김경진;윤용달;이정주;조완규
    • The Korean Journal of Zoology
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    • v.33 no.2
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    • pp.152-157
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    • 1990
  • The present study, using immunohistochemical procedure, was carried out to determine the localization of immunostainable $\beta$-endorphin cells in the mouse ovarian tissues. Mature female mice were perfused with 4% neutral buffered paraformaldehyde under anesthesia and then frozen-sections were immunostained with anti $\beta$-endorphin antiserum according to ABC technique. Immunoreactive $\beta$-endorphin was found in the luteal cells of corpus lutea, but not in the thecal cells. More strong immunostaining signak were observed in large corpus luteum, in particular, the regressing luteal cells. Primary and secondary follicles did not show any immunoreactivity of $\beta$-endorphin, but granulosa cells lining the antral cavity of large antral follicles contained immunoreactive $\beta$-endorphin.

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Comparison Study of Thermal Decomposition Characteristics of Wattle & Pine Tannin-based Adhesives

  • Kim, Sumin;Lee, Young-kyu;Kim, Hyun-Joong;Eom, Young Geun
    • Journal of the Korean Wood Science and Technology
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    • v.30 no.3
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    • pp.34-41
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    • 2002
  • This study investigated the thermogravimetric analysis of two types of cured tannin-based adhesives from wattle and pine, with three hardeners of paraformaldehyde, hexamethylenetetramine and TN (tris(hydroxyl)nitromethan), at a temperature of 170℃ and a heating rate of 5, 10, 20 and 40℃/min for 10 minutes. The 5 minutes cured wattle tannin-based adhesive with each hardener at 170℃ was also tested to compare the degree of curing. It was found that thermogravimetric analysis could be used to measure the degree of curing of a thermosetting adhesive. The TG-DTG curves of all the adhesive systems were similar and showed three steps in a similar way to a phenolic resin. This means that each adhesive system is well cross-linked. However, a high thermal decomposition rate was shown at 150 to 400℃ in the case of the pine tannin sample with TN (tris(hydroxyl)nitromethan). The Flynn & Wall expression was used to evaluate the activation energy for thermal decomposition. As the level of conversion (𝛼) increased, the activation energy of each system increased. The activation energy of the wattle tannin-based adhesive with paraformaldehyde was higher than the others.

Methanol fixation for scanning electron microscopy of plants

  • Ki Woo Kim
    • Applied Microscopy
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    • v.50
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    • pp.10.1-10.6
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    • 2020
  • Plant specimens for scanning electron microscopy (SEM) are commonly treated using standard protocols. Conventional fixatives consist of toxic chemicals such as glutaraldehyde, paraformaldehyde, and osmium tetroxide. In 1996, methanol fixation was reported as a rapid alternative to the standard protocols. If specimens are immersed in methanol for 30 s or longer and critical-point dried, they appear to be comparable in preservation quality to those treated with the chemical fixatives. A modified version that consists of methanol fixation and ethanol dehydration was effective at preserving the tissue morphology and dimensions. These solvent-based fixation and dehydration protocols are regarded as rapid and simple alternatives to standard protocols for SEM of plants.

Bonding Quality of Cylindrical LVL and Surface Durability by Its Painting (원통형 단판적층재의 접착성 및 도장처리에 따른 표면내구성)

  • Suh, Jin-Suk;Kim, Jong-In;Hwang, Sung-Wook;Park, Sang-Bum
    • Journal of the Korean Wood Science and Technology
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    • v.40 no.6
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    • pp.418-423
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    • 2012
  • In order to develop the end use of cylindrical laminated veneer lumber (LVL) such as wooden crafts, the water proof-bonding strength, the resistance to abrasion and the surface hardness by painting the surface of LVL were investigated. The study results were as follows; The water proof-bonding strength through 5 cyclic test by boiling in water immersion and drying were favorable without delamination of glue line. Then the formulation of glue was resorcinol resin (100) to hardener of paraformaldehyde (5) by mixed weight percentage. The resistance to abrasion was relatively higher at cross section than tangential section. When tangential section of LVL was painted by UV protection oil, the resistance to abrasion was improved. In case of an cross section of LVL, the higher surface hardness appeared at larch core than radiata pine LVL. Also, in case of an tangential section of LVL, the higher surface hardness appeared at glue line than veneer side.

Fine structure of Laminaria religiosa Miyabe (Laminariales, Phaeophyta) 1 . Composition of Fixatives. (애기다시마 식물의 미세구조 연구 1. 고정액 조성)

  • CHUNG Ik-Kyo
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.23 no.2
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    • pp.155-166
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    • 1990
  • There have been many problems like plasmolysis in the ultrastructural studies with kelps. The present study was conducted with series of different fixatives and buffer solutions and several resins to solve the plasmolysis phenomena. The fixative of 1470 mosmol($3\%$ glutaraldehyde, $2.4\%$ paraformaldehyde in 0.05M cacodylate buffer with 0.2M sucrose and $0.5\%$ caffeine at pH of 7.2) showed the least frequency of plasmolysis. The relationship between the concentration of primary fixatives and the frequency of plasmolysis was not found. With above fixative the possibility of plasmolysis during infiltration period was not identified using ultra-low viscosity resin. Generally, the kelp(Laminaria religiosa) contains a lot of mucilage, which interupts the infiltration of fixative. The possible physiological mechanisms related with mucilage secretion may be unable to fix the region of plasmalemma properly.

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The Synthesis of Carbamoylsulfenyl Chloride and its Derivatives (염화카바모일술페닐과 그 유도체의 합성)

  • Surk Sik Moon;Dong Young Oh
    • Journal of the Korean Chemical Society
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    • v.27 no.2
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    • pp.157-164
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    • 1983
  • N-Methylidene-2,6-diethylaniline (III) was prepared by the reaction of 2,6-diethylaniline (II) with an excess paraformaldehyde. The protons of N=$CH_2$in the compound III exhibited a second order NMR spectrum. The compound III reacted with bifunctional chlorocarbonylsulfenyl chloride(IV) to give N-(chloromethyl)-N-(2,6-diethylphenyl)-carbamoylsulfenyl chloride(V). The reaction of the compound V with various alcohols resulted in the formation of Alkyl N-(chloromethyl)-N-(2,6-diethylphenyl)-carbamoylsulfenate esters(VI${\sim}$XVI) in 71${\sim}$95% yields. The compound VI${\sim}$XVI decomposed gradually, but the thermal rearrangement of S-O bonding to S=O bonding was not found. The nucleophilic attack of an excess alcohol to the compound Ⅴ was made on both divalent sulfur and ${\alpha}$-carbon of N-chloromethyl group. The corresponding substituted products(XVII, XVIII) had considerable stability.

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Expression of UT-A in Rat Kidney: Ultrastructural Immunocytochemistry (흰쥐 콩팥에서 요소운반체-A의 발현: 미세구조적 면역세포화학법)

  • Lim, Sun-Woo;Jung, Ju-Young;Kim, Wan-Young;Han, Ki-Hwan;Cha, Jung-Ho;Chung, Jin-Woong;Kim, Jin
    • Applied Microscopy
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    • v.32 no.2
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    • pp.91-105
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    • 2002
  • Urea transport in the kidney is mediated by a family of transporter proteins that includes renal urea transporters (UT-A) and erythrocyte urea transporters (UT-B). The cDNA of five isoforms of rat UT-A, UTA1, UT-A2, UT-A3, UT-A4, and UT-A5 have been cloned. The purpose of this study was to examine the expression of UT-A (L194), which marked UT-A1, UT-A2 and UT-A4. Male Sprague-Dawley rats, weighing approximately 200 g, were divided into three group: control rats had free access to water, dehydrated rats were deprived of water for 3 d, and water loaded rats had free access to 3% sucrose water for 3 d before being killed. The kidneys were preserved by in vivo perfusion through the abdominal aorta with the 2% paraformaldehyde-lysine- periodate (PLP) or 8% paraformaldehyde solution for 10 min. The sections were processed for immunohistochemical studies using pre-embedding immunoperoxidase method and immunogold method. In the normal rat kidney, UT-A1 was expressed intensely in the cytoplasm of the inner medullary collecting duct (IMCD) cell and UT-A2 was expressed on the plasma membrane of the terminal portion of the shortloop descending thin limb (DTL) cells (type I epithelium) and of the long-loop DTL cells (type II epithelium) in the initial part of the inner medulla. Immunoreactivity for UT-A1 in the IMCD cells, was decreased in dehydrated animals whereas strongly increased in water loaded animals compared with control animals. In the short-loop DTL, immunoreactivity for UT-A2 was increased in intensity in both dehydrated and water loaded groups. However, in the long-loop DTL of the outer part of the inner medulla, immunoreactivity for UT-A2 was markedly increase in intensity in dehydrated group, but not in water loaded group. In conclusion, in the rat kidney, UT-A1 is located in the cytoplasm of IMCD cells, whereas UT-A2 is located in the plasma membrane of both the short-and long-loop DTL cells. Immunohistochemistry studies revealed that UT-A1 and UT-A2 may have a different role in urea transport and are regulated by different mechanisms.

Treatment Characteristics of Plating Wastewater Containing Freecyanide, Cyanide Complexes and Heavy Metals (II) - Effect of Aldehyde Compounds and Polysulfide - (도금폐수내 유리시안과 착염시안 및 중금속의 처리특성 (II) - aldehyde와 polysulfide첨가에 따른 영향 -)

  • Jung, Yeon-Hoon;Lee, Soo-Koo
    • Journal of Korean Society on Water Environment
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    • v.26 no.4
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    • pp.687-690
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    • 2010
  • The objective of this study is to investigate the effect of aldehyde compounds and ploysulfide as accelerating agents on removal of heavy metals and CN in plating wastewater. As a results of the experiments, the removal efficiency of cyanide using the formaldehyde type of aldehydes was the highest at pH 9. Next types were sodium formaldehyde bisulfite addut> paraldehyde> paraformaldehyde. Also, optimum pH and dosage for treating the residual heavy metals by using polysulfide were pH 9 and 30 mg/L, respectively. The removal efficiencies of cyanide, chromium, zinc and copper were above 96.7% at optimum condition.

Pulpal and Periapical Reaction to Formocresol and Depulpin in Pulpotomized Rat Teeth.

  • Moon, Hyung-In;Whang, In-Nam;Kim, Sun-Hun;Oh, Won-Mann
    • Proceedings of the KACD Conference
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    • 2001.11a
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    • pp.583.1-583
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    • 2001
  • One fifth dilution of formocresol is usually used for pulpotomy of primary teeth and emergency pulpotomy of permanent teeth. Recently Depulpin (VoCo., Germany) gains popularity as devitalizing agent during root canal therapy in spite of high concentration of 49% paraformaldehyde. But there are not enough publications about the reaction of dental pulp and periapical tissue caused by Depulpin. Therefore, This study was performed to evaluate the histological changes in pulp and periapical tissue of rat after pulpotomy using formocresol and Depulpin.(omitted)

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