• The Transactions of the Korea Information Processing Society
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• v.6 no.2
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• pp.292-298
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• 1999

An information structure of large size hypermedia application is usually hierarchical, and the sibling nodes in this structure have same or similar tags and contents. a word "개그" that means the common set of sibling nodes in the hierarchical information structure is used in this paper. It proposes a design method that divides form and content from nodes and it proposes HTML page generation algorithm from forms and contents. This method has reusability of form, maintainability of documents and reduction of cost for authoring.

• The Journal of the Korea Contents Association
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• v.14 no.9
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• pp.141-152
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• 2014

The user interfaces of electronic-book (e-book) readers in the e-book market are highly diverse, and this has led to major usability issues. In this paper, we analyze user preferences in terms of feature-setting options and the use of touchscreen actions in the six most commonly used e-book readers for smartphnoes. We identify alternatives for these features, which can enhance the usability of e-book readers, based on these user preferences. The survey results for the feature-setting options show that it is desirable to support at least eight background colors, as well as the ability to specify the color of the background icons. Adjusting the screen brightness using a setting bar with the support of an auto-brightness option is desirable, as in using +/- buttons to adjust the font size, as well as approximately 10 font faces. We find that it is desirable to support fade, slide, scroll and curl page-turing options, in addition to a simple non-animated page-turning effect, and that page movement should be accomplished using a scroll bar with the support of the page movement features by entering a page number, and by using the table of contents as well as bookmarks. The survey results on the use of touchscreen options indicated that it is useful to be able to configure the screen layout of an e-book reader by dividing it into three areas: left, right, and center. And then, when users briefly touch the left and right areas, it is ideal to move to the previous and subsequent pages, respectively; and when they briefly touch the center region, it is desirable to display a touch feature menu. We believe that the results of this study may provide guidance in the design of user interfaces for e-book readers.

• Journal of KIISE:Computer Systems and Theory
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• v.33 no.5
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• pp.306-315
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• 2006

In general, the hit ratio of the first level cache is one of the most important factors in determining the performance of computer systems. Prefetching from lower level memory structure is one of the most useful techniques for improving the hit ratio of the first level cache. In this paper, we propose a prefetch on continuous same page access (CSPA) scheme which improves the prefetch efficiency of the instruction cache and reduces prefetch cost at the same time. The proposed CSPA scheme traces the page addresses of executed instructions to count how many times the same memory page is accessed continuously. To increase the prefetch efficiency, the CSPA scheme initiates prefetch only if the number of accesses to the same page exceeds the threshold value. Generally, the size of a L1 cache block is smaller than that of a L2 cache block. Therefore, one L2 cache block contains a number of L1 cache blocks. To reduce the number of unnecessary accesses to the L2 cache due to prefetch, the CSPA scheme enables prefetch only when the missed L1 block and the prefetch L1 block are in the same L2 cache block, leading to reduced prefetch cost. According to our simulations, the proposed prefetching scheme improves the performance by up to 6.7%.

• Journal of Microbiology and Biotechnology
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• v.14 no.3
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• pp.620-627
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• 2004

On the foundation of a database of genome sequences and protein analyses, the ability to clone a gene based on a peptide analysis is becoming more feasible and effective for identifying a specific gene and its protein product of interest. As such, the current study conducted a protein analysis using 2-D PAGE followed by MALDI- TOF and ESI-MS to identify a highly expressed gene product of C. parasitica. A distinctive and highly expressed protein spot with a molecular size of 47.2 kDa was randomly selected and MALDI-TOF MS analysis was conducted. A homology search indicated that the protein appeared to be a fungal enolase (enol). Meanwhile, multiple alignments of fungal enolases revealed a conserved amino acid sequence, from which degenerated primers were designed. A screening of the genomic $\lambda$ library of C. parasitica, using the PCR amplicon as a probe, was conducted to obtain the full-length gene, while RT-PCR was performed for the cDNA. The E. coli-expressed eno 1 exhibited enolase enzymatic activity, indicating that the cloned gene encoded the C. parasitica enolase. Moreover, ESI-MS of two of the separated peptides resolved from the protein spot on 2-D PAGE revealed sequences identical to the deduced sequences, suggesting that the cloned gene indeed encoded the resolved protein spot. Northern blot analysis indicated a consistent accumulation of an eno1 transcript during the cultivation.

• Journal of Pharmaceutical Investigation
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• v.31 no.3
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• pp.191-196
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• 2001

Biodegradable polymeric microspheres were studied for their usefulness as carriers for the delivery of vaccine antigens. However, protein antigen could be denatured during microencapsulation processes due to the exposure to the organic phase and stress condition of cavitation and shear force. Therefore this study was carried out to re-evaluate the degree of protein denaturation during microencapsulation with poly(lactide-co-glycolide) (PLGA) copolymer. PLGA microspheres containing ovalbumin (OVA), prepared by W/O/W multiple emulsification method, were suspended in pH 7.4 PBS and incubated with shaking at $37.5^{\circ}C$. Drug released medium was collected periodically and analyzed for protein contents by micro-BCA protein assay. In order to evaluate the protein integrity, release medium was subjected to the analyses of SDS-PAGE and size exclusion chromatography (SEC). And enzyme-linked immunosorbent assay (ELISA) was introduced to measure the immunoreactivity of entrapped OVA and to get an insight into the three-dimensional structure of epitope. The structures of entrapped protein were not affected significantly by the results of SDS-PAGE and SEC. However, immunoreactivity of released antigen was varied, revealing the possibility of protein denaturation in some microspheres when it was evaluate by ELISA method. Therefore, in order to express the degree of protein denaturation, antigenicity ratio (AR) was obtained as follows: amount of immunoreactivity of OVA/total amount of OVA released ${\times}100(%)$. ELISA method was an efficient tool to detect a protein denaturation during microencapsulation and the comparison of AR values resulted in more accurate evaluation for immunoreactivity of entrapped protein.

• The Transactions of the Korea Information Processing Society
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• v.7 no.1
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• pp.273-285
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• 2000

While page segmentation is an important step in document recognition, there haven's been many researches on it. More improvement is still needed on the segmentation of document elements in complicated or color documents. In this paper, I present a new page segmentation method which can segment pages with multiple columns, dotted lines, graphics, and photographs. I extract all connected components using contour following and combine them depending on the size and positional information of them. Separate text location is done for non-text color regions to extract possible text lines. To see the performance of the proposed method, experiments are done for 180 documents. Four commercial OCR programs are also tested and the proposed method showed the best result.

• Journal of the Korea Convergence Society
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• v.12 no.2
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• pp.147-154
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• 2021

This study focused on the fitting window structure provided by the virtual fitting service web page. We analyzed the current cases and identified the user's needs for the service through usability assessment, survey, and FGI experiments. As a result of the design, the fitting window of the existing web page was opened at the same time as the site was accessed to improve the hassle of using the icons due to their small size and poor visibility. In the case of fitting windows, the problem of information delivery was supplemented so that even the initial user could understand the fitting map of the existing method, and additional items needed for user propensity were provided so that the service could be used accurately and easily. As a result of this study, it can be used as a basic research material in future virtual fitting service studies and is expected to provide good implications to fashion marketers.

• Journal of fish pathology
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• v.6 no.2
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• pp.87-92
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• 1993

In February of 1990, an epizootic disease to masu salmon. Onchorynchus masou cultured at the hatchery of trout in Samchuk. Kwangwondo have broken out and induced heavy mortality. An infectious hematopoietic necrosis virus(IHNV) was isolated from diseased masu salmon fry by the use of fish cell line, CHSE-214. This IHNV isolated from masu salmon was compared with USA isolates of IHNV, SRCV and RB-76 by analysis of virion proteins in sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) and neutralization tests with two monoclonal antibodies raised against SRCV(MAb SRCV/A4) and RB-76(MAb RB/B5). In the antigenicity and the size of structural proteins. this IHNV, SCS atrain was smilar to RB-76 belonged to the electropherotype I proposed by Hsu et al.(1986).

• Journal of the Korean Wood Science and Technology
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• v.35 no.5
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• pp.100-108
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• 2007

$\beta$-Glucosidase from Laetiporus sulphureus among the enzymes related to lignocellulosic biomass degradation to sugars for using alternative bioethanol production was characterized. The highest activity of $\beta$-glucosidase was obtained on cellobiose at shaking culture. For the characterization and purification of $\beta$-glucosidase culture solution was concentrated and then purified by FPLC using ion exchange and size exclusion column. According to the results of SDS-PAGE, native PAGE and microfluidic system of purified enzyme, protein band was observed at about 132 kDa. Optimal pH and temperature of purified $\beta$-glucosi-dase were 5.0 and $60^{\circ}C$, respectively. In the kinetic properties of $\beta$-glucosidase on various substrates such as sophorose, gentiobiose and cellobiose, $K_m$ was 0.81, 1.07 and 1.70 mM, respectively.

• Korean Journal of Microbiology
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• v.30 no.6
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• pp.501-506
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• 1992

An alkalophilic Vibrio sp. RH530 showing high proteolytic activity was isolated form soil samples by enrichment culture. The activity staining using gelatin SDS- polyacrylamide gel electrophoresis (PAGE ) revealed that the strain produced an alkaline major protease (Apr B) with a size of 27 kDa, and at least six minor proteases. The apparent sizes of four of the minor proteases were approximately 45, 28, 22 and 19 kDa. Apr B and five of the minor proteases were inhibited by serine protease inhibitors including PMSF and DFP, suggesting that they are serine proteases. One of the minor proteases was inhibited by metalloprotease inhibitors, not by serine protease inhibitors, indicating it to be a metalloprotease. Furthermore, the activities of Apr B and Prt 3 were not inhibited by SDS in the reaction mixture. The production of Apr B and some of the minor proteases was specifically affected by culture temperature (30 to 37.deg.C) and pH (7 to 10). The production of Apr B. Prt 2, Prt 5 and Prt 6 was mainly affected by culture temperature, while Prt 4 by culture pH. Prt 1 and Prt 3 were not affected by neither of these factors.