Journal of Pharmaceutical Investigation
- Volume 31 Issue 3
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- Pages.191-196
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- 2001
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- 2093-5552(pISSN)
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- 2093-6214(eISSN)
Antigenicity of Protein Entrapped in Poly(lactide-co-glycolide) Microspheres
폴리락티드-글리콜리드 마이크로스피어에 봉입된 단백질의 항원성 평가
- Song, Seh-Hyon (College of Pharmacy, Chung-Ang University) ;
- Cho, Seong-Wan (College of Pharmacy, Chung-Ang University) ;
- Shin, Taek-Hwan (College of Pharmacy, Chung-Ang University) ;
- Yoon, Mi-Kyoung (College of Pharmacy, Chung-Ang University) ;
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Choi, Young-Wook
(College of Pharmacy, Chung-Ang University)
- Published : 2001.09.20
Abstract
Biodegradable polymeric microspheres were studied for their usefulness as carriers for the delivery of vaccine antigens. However, protein antigen could be denatured during microencapsulation processes due to the exposure to the organic phase and stress condition of cavitation and shear force. Therefore this study was carried out to re-evaluate the degree of protein denaturation during microencapsulation with poly(lactide-co-glycolide) (PLGA) copolymer. PLGA microspheres containing ovalbumin (OVA), prepared by W/O/W multiple emulsification method, were suspended in pH 7.4 PBS and incubated with shaking at