• Korean Journal of Plant Resources
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• v.36 no.5
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• pp.446-454
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• 2023

This study aimed to assess the potential use of Paeonia lactiflora Pall. seed cake (PSC). The extraction yield of the PSC extract ranged from 22% to 45%, depending on the extraction solvent used. The PSC extract showed significantly higher levels of total polyphenols and flavonoids contents, and radical scavenging compared to the P. lactiflora seed oil (PSO) extract. The antibacterial activity of the PSC extract was superior to that of the positive control and remained effective for up to 48 hours. Furthermore, when the PSC extract was applied, it significantly reduced the inflammatory response induced by LPS, demonstrating the anti-inflammatory activity of PSC. This study confirmed the effective bioactivity not only in PSO, but also in the PSC extract, highlighting the potential of PSC as a bio-health ingredient.

• Korean Journal of Pharmacognosy
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• v.52 no.3
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• pp.157-162
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• 2021

The heat-mediated reflux apparatus extraction of Paeonia lactiflora Pall. has been widely used as a traditional extraction method. In this paper, the microwave apparatus extraction method of Paeonia Radix was performed and the active ingredients and functional constituents were compared with the reference extraction method. The most effective extraction condition of albiflorin using the microwave was 120℃ in 50% methanol, and paeoniflorin was maximally extracted at 60℃. The reduced level of paeoniflorin molecule at high-pressure and high-temperature extraction condition was caused by the molecular instability. Additionally, the microwave extraction of 50% methanol extracts at 150℃ showed the highest functional constituents determined by in vitro DPPH radical scavenging activity, polyphenol concentration, and tyrosinase inhibition assay. The microwave apparatus was adapted as a rapid, low-cost, and environmentally friendly method to extract active ingredients and the practical extraction conditions of Paeonia Radix can be used in industrial applications.

• Archives of Pharmacal Research
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• v.26 no.10
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• pp.832-839
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• 2003

Activated macrophages express inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase (COX-2), and produce excessive amounts of nitric oxide (NO) and prostaglandin E$_2$ (PGE$_2$), which play key roles in the processes of inflammation and carcinogenesis. The root of Paeonia lactiflora Pall., and the root cortex of Paeonia suffruticosa Andr., are important Chinese crude drugs used in many traditional prescriptions. 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose (PGG) is a major bioactive constituent of both crude drugs. PGG has been shown to possess potent anti-oxidant, anti-mutagenic, anti-proliferative and anti-invasive effects. In this study, we examined the inhibitory effects of 1,2,3,4,6-penta-O-galloyl-$\beta$-D-glucose (PGG) isolated from the root of Paeonia lactiflora Pall. on the COX-2 and iNOS activity in LPS-activated Raw 264.7 cells, COX-1 in HEL cells. To investigate the structure-activity relationships of gallate and gallic acid for the inhibition of iNOS and COX-2 activity, we also examined (-)-epigallocatechin gallate (EGCG), gallic acid, and gallacetophenone. The results of the present study indicated that PGG, EGCG, and gallacetophenone treatment except gallic acid significantly inhibited LPS-induced NO production in LPS-activated macrophages. All of the four compounds significantly inhibited COX-2 activity in LPS-activated macrophages. Among the four compounds examined, PGG revealed the most potent in both iNOS ($IC_{50}$ = 18 $\mu\textrm{g}/mL$) and COX-2 inhibitory activity (PGE$_2$: $IC_{50}$ = 8 $\mu\textrm{g}/mL$ and PGD$_2$: $IC_{50}$ = 12 $\mu\textrm{g}/mL$), respectively. Although further studies are needed to elucidate the molecular mechanisms and structure-activity relationship by which PGG exerts its inhibitory actions, our results suggest that PGG might be a candidate for developing anti-inflammatory and cancer chemopreventive agents.

• Korean Journal of Medicinal Crop Science
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• v.2 no.2
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• pp.149-153
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• 1994

This experiment was conducted to know the relationship between root diameter and the contents of paeoniflorin and some Chemical components in Paeoniae radix. Paeoniae radix of Euisung cultivar was harvested on 17 June, 1993 and divided into four gorups according to root diameter such as $30{\pm}2mm,\;17{\pm}1mm,\;12{\pm}1mm\;and\;7{\pm}1mm$. Paeoniflorin, total sugars, starch, crude protein, crude fat and crude ash of them were analyzed after dryed them for 30 days in room temperature. The ratio of shrinkage with different root diameter were not different significantly, and the average ratio of them was about 30%. Contents of paeoniflorin of $17{\pm}1mm$ root diameter was lower than that of $7{\pm}1mm$. but contents of total sugar and starch of $17{\pm}1mm$ were higher than those of $7{\pm}1mm$. Paeoniflorin contents was positively correlated with crude protein and crude fiber, and crude protein contents was negatively correlated with total sugars and starch.

• Journal of Plant Biotechnology
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• v.49 no.3
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• pp.207-212
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• 2022

The optimal culture conditions for root organogenesis from the callus of peonies (Paeonia lactiflora Pall.) were investigated. Root explants with vascular bundles were cultured in Murashige and Skoog (MS) medium combined with 0.5-4.0 mg/L auxins (indole acetic acid [IAA], naphthalene acetic acid [NAA], indolebutyric acid [IBA], and 2,4-dichlorophenoxyacetic acid [2,4-D]) and 0.0-2.0 mg/L cytokinins (kinetin, zeatin, and benzylaminopurine [BAP]) to induce callus formation. The callus was then cultured in MS medium combined with three concentrations (0.1, 0.5, and 1.0 mg/L) of IAA, NAA, IBA, kinetin, zeatin, and BAP in the dark for 6 weeks. Based on the results, the effects of dark and light conditions on the callus cultured in MS medium with combinations of 0.1-1.0 mg/L IBA and zeatin for 6 weeks were studied. Callus formation was most effective (>+++) in the medium with a combination of 1.0 mg/L NAA and 1.0 mg/L zeatin. A high number of long adventitious roots were observed in the mediums with 0.1 mg/L IBA (6.66 and 4.82 cm) and 0.5 mg/L zeatin (2.32 and 0.72 cm) among auxins and cytokinins, respectively. The highest number (14.06) of adventitious roots were formed from the callus cultured in light in the MS medium combined with 0.1 mg/L IBA and 0.5 mg/L zeatin. This same medium induced the formation of the longest adventitious root (5.45 cm) in the dark. Thus, optimization of in vitro culture conditions may be possible for the mass propagation of adventitious roots in peonies.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.39 no.4
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• pp.397-404
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• 1994

This experiment was conducted to determine best harvesting time and observe paeoniflorin and some chemical component concentrations of 4-year-old paeonia radix in Euisung and Milyang varieties. Concentrations of paeoniflorin and some chemical components were investigated at 8 periods from Feb. to Oct. Yield of paeonia radix was investigated in Aug. 19, Sep. 18 and Oct. 17. Paeoniflorin and some chemical components were changed seriously at May as flowering time and stabilized after June. The lowest dry matter ratio was 23% and the maximum shrinkage ratio was 47% in May during all the growing stages. Especially, the difference of paeoniflorin concentrations between Euisung and Milyang was about 2%, and paeoniflorin concentrations in Milyang were higher than in Euisung variety during all the stages. Serious infection of disease in October, stem length, stem number per stock and healthful stem ratio in October were more reduced than in August. Yield of paeonia radix was not different from August to September, but that of October harvest was obviously reduced by root-decay-disease. Reasonable harvesting time in 4-year-old paeonia radix was late August to mid-September. When infected shoot rate by disease were severely high, early harvest was more stable in the yield and quality aspects.

• Journal of the Korean Society of Food Science and Nutrition
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• v.32 no.7
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• pp.1102-1107
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• 2003

To study the effects of substances in paeonia seeds (Paeonia lactiflora Pall.) on lipid metabolism, crude methanol extract and secondary ether-soluble fraction out of defatted methanol extract and trans-resveratrol were prepared from the seeds and added to 0.5% (w/w) cholesterol diets for rats. Male Sprague-Dawley rats weighing 120$\pm$11 g were divided into six experimental groups: control group with no extra supplement, 0.1% (MP1) and 0.2% methanol extract (MP2) supplemented groups, 0.05% (EP1) and 0.1% ether-soluble fraction (EP2) supplemented groups and 0.02% resveratrol supplemented group. Experimental diets were fed ad libitum to the rats for 3 weeks. Body weight gains and food efficiencies were not different among the six experimental groups. Relative liver weights were lower in EP2 group compared to those in control group, but serum GOT and GPT levels of paeonia seed groups including trans-resveratrol group were not different from those of the control group. Serum total cholesterol levels reduced in EP2 and resveratrol groups but HDL-/total cholesterol ratios significantly increased in the four paeonia groups except EP1 group compared with the control group and serum triglyceride level lowered only in EP2 group than that of the control. However, liver cholesterol levels lowered in the five paeonia groups but triglyceride level lowered in MP2, EP1, EP2 groups than that of the control group. Fecal cholesterol excretion significantly increased in MP2, EP1, and EP2 groups than that of the control group, but bile acid excretions were not changed except that a reduction in EP2 group. These results suggest that paeonia seeds contain substances improving serum lipid status mostly via HDL pathway and resveratrol as monomer is one of the effective components but others including resveratrol oligmer are involved in the lipid improving effect.

• Archives of Pharmacal Research
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• v.25 no.3
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• pp.293-299
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• 2002

Cytotoxic and antimutagenic effects of a novel cis-$\varepsilon$-viniferin and five known stilbenes, transresveratrol, trans-$\varepsilon$-viniferin, gnetin H, suffruticosols A and B, isolated from the seeds of Paeonia lactiflora Pall. (Paeoniaceae) were determined against five different cancer cell lines, and mutagenicity of N-methyl-N'-nitro-N-nitrosoguanidine (MNNG) in Salmonella typhimurium TA100, respectively. Six stilbenes showed cytotoxic activity in a dose-dependent manner, and especially did potent cytotoxic activity against C6 (mouse glioma) cancer cell with $IC_{50}$ values ranging from 8.2 to $20.5{\;}{\mu\textrm{g}}/ml$. trans-Resveratrol showed significant cytotoxic activity against HepG2 (liver hepatoma) and HT-29 (colon) human cancer cell lines with $IC_{50}$ values of 11.8 and 25.2 g/ml, respectively. In contrast, trans-$\varepsilon$-viniferin and cis--viniferin, and gnetin H exhibited marked cytotoxic activity against Hela (cervicse) and MCF-7 (breast) human cancer cell lines with $IC_{50}$ values of 20.4, 21.5, and $12.9{\;}{\mu\textrm{g}}/ml$, respectively. However, suffruticosol A and B had less cytotoxic effect against all cancer cells except C6. Meanwhile, six stilbenes exerted antimutagenic activity in a dose-dependent fashion. Of them, trans-resveratrol exhibited the strongest antimutagenic effect against MNNG with $IC_{50}$ value of $27.0{\;}{\mu\textrm{g}}/plate$, while other five resveratrol oligomers also did moderate antimutagenic activity with $IC_{50}$ values ranging from 31.7 to $35.2{\;}{\mu\textrm{g}}/plate$.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.51 no.spc1
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• pp.251-254
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• 2006

This study was conducted to identify changes of chemical components affected by different drying method and temperature conditions in leaves and stems of peony plant. Drying methods were the dried air heated $(50^{\circ}C)$, far-red ray $(50^{\circ}C)$, room temperature and oven dry $(50^{\circ}C)$. Drying temperature were 40, 50, 60, 70 and $80^{\circ}C$ on far-red ray dryer. Among the drying methods, the contents of components were the highest in far-red drying and normal temperature drying as compared with air heated drying and oven drying. Among the drying temperature conditions, the contents of components were the highest in drying temperature at $40^{\circ}C$ and decreased in high temperature of $70^{\circ}C\;and\;80^{\circ}C$.

• Korean Journal of Plant Resources
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• v.19 no.1
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• pp.83-87
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• 2006

Study on the effective cultivation method for earlier harvest of peony(Paeonia lactiflora Pall.), was carried out in Uiseong Medicinal Plant Experiment Station from 2001 to 2003. Sprouting time and flowering time in house cultivation were earlier than in field cultivation. Planting large crowns of $100{\sim}130g$ increased yield by 34%. The method that is 'Large crown($100{\sim}130g$) + dense planting + vinyl mulching for 2 years' resulted good growth and 54% yield increase. This method made it possible to harvest peony on the second year and reduce productive cost. The yield was decreased more or less in this method but yearly income was increased by 28%.