• Title/Summary/Keyword: pK$_a$ Values

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Biokinetics of Carbohydrate and Lipid Metabolism in Normal Laying Hen [Part 1] -Determination of Turnover of Glucose- (정상산란계(正常産卵鷄)에 있어서 탄수화물(炭水化物)과 지질대사(脂質代謝)의 생동역학(生動力學) 제1보[第一報] -포도당 대사회전(代謝回轉)의 측정(測定)-)

  • Chiang, Y.H.;Riis, P.M.
    • Applied Biological Chemistry
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    • v.20 no.2
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    • pp.205-209
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    • 1977
  • The pool size of plasma glucose, turnover rate and other concerned items for glucose metabolism in normal laying hen were investigated by a single-injection method using $U-C^{14}-glucose$. The 11.6 nCi of pure dose was injected to a hen normally fed through the wing vein. The glucose concentration in plasma sample taken at 5 minutes after injections was 214mgper 100ml. From the plottings of logarithmic standard specific activities of plasma taken from 5 to 120 minutes against the time after injection and from the regresion analysis, metabolic states were determined. The pool size was 1.07g, turnover rate was 0.024 per minute, turnover time was 41 minutes, utilization rate was 26mg/min. (0.83 g/hr/kg B.W. 3/4) and glucose space(extracellular fluid volume) was 25.3 per cent of body weight. The values obtained from. 10-50 minutes samples were similar to those described above, which we from 5-120 minutes samples.

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Phytochemical compounds and quality characteristics of spray-dried powders with the blanching condition and selected forming agents from pressed extracts of Ligularia fischeri leaves (블랜칭 처리 및 부형제 종류에 따른 곰취 착즙액 분무건조 분말의 phytochemical 성분 및 품질특성)

  • Kim, Jae-Won;Park, In-Kyung;Youn, Kwang-Sup
    • Food Science and Preservation
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    • v.20 no.5
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    • pp.659-667
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    • 2013
  • This study was performed to determine the effects of the blanching condition (immersion ratio 1:15 (w/v) for 3 min at $95^{\circ}C$, and solution containing 1% sodium chloride) and selected forming agents (dextrin DE=10, dextrin DE=20, ${\beta}$-cyclodextrin; each forming agents added 5%) on the phytochemical compounds and quality characteristics of Ligularia fischeri leaves. The moisture was not affected by the forming agent. The color of a, b and chroma values were low in the blanching treatment groups and were significantly lowest with ${\beta}$-cyclodextrin (CD). The polyphenol and flavonoid contents in the blanching treatment groups were higher than those in the non-blanching-treatment group. The ascorbic acid content was higher in the non-blanching-treatment group and was significantly highest in the group treated with dextrin (DE=10) whereas the blanching treatment groups showed lower dehydroascorbic acid content than the non-blanching-treatment group. The water absorption was higher in the non-blanching-treatment group and was significantly highest in the group treated with CD. The water solubility in the blanching treatment groups treated with dextrin (DE=20) and CD was higher than that in the blanching treatment group treated with DE=10. The total chlorophyll and chlorophyll a and b contents were high in the blanching treatment group treated with CD, and for the total carotenoid contents, the same tendency as that seen with the chlorophyll content was observed. With regard to the particle diameter, those in the blanching treatment groups were lower than that in the non-blanching-treatment group and was significantly lowest in the blanching treatment groups treated with DE=20 and CD. The result of SEM observation showed that the spray-dried powders in blanching treatment groups treated with the DE=20 and CD forming agents had uniform particle distribution.

Index-based Searching on Timestamped Event Sequences (타임스탬프를 갖는 이벤트 시퀀스의 인덱스 기반 검색)

  • 박상현;원정임;윤지희;김상욱
    • Journal of KIISE:Databases
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    • v.31 no.5
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    • pp.468-478
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    • 2004
  • It is essential in various application areas of data mining and bioinformatics to effectively retrieve the occurrences of interesting patterns from sequence databases. For example, let's consider a network event management system that records the types and timestamp values of events occurred in a specific network component(ex. router). The typical query to find out the temporal casual relationships among the network events is as fellows: 'Find all occurrences of CiscoDCDLinkUp that are fellowed by MLMStatusUP that are subsequently followed by TCPConnectionClose, under the constraint that the interval between the first two events is not larger than 20 seconds, and the interval between the first and third events is not larger than 40 secondsTCPConnectionClose. This paper proposes an indexing method that enables to efficiently answer such a query. Unlike the previous methods that rely on inefficient sequential scan methods or data structures not easily supported by DBMSs, the proposed method uses a multi-dimensional spatial index, which is proven to be efficient both in storage and search, to find the answers quickly without false dismissals. Given a sliding window W, the input to a multi-dimensional spatial index is a n-dimensional vector whose i-th element is the interval between the first event of W and the first occurrence of the event type Ei in W. Here, n is the number of event types that can be occurred in the system of interest. The problem of‘dimensionality curse’may happen when n is large. Therefore, we use the dimension selection or event type grouping to avoid this problem. The experimental results reveal that our proposed technique can be a few orders of magnitude faster than the sequential scan and ISO-Depth index methods.hods.

MICROTENSILE BOND STRENGTH OF DENTIN BONDING ADHESIVES ON BOVINE TEETH (Bovine teeth에 대한 수 종 상아질 접착제의 미세인장결합강도)

  • Song, Eun-Ju;Kim, Jae-Moon;Kim, Shin;Jeong, Tae-Sung
    • Journal of the korean academy of Pediatric Dentistry
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    • v.34 no.3
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    • pp.420-429
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    • 2007
  • The purpose of this study was to compare the micro ensile bond strength to bovine dentin of several adhesives (SM, Scotch $Bond^{TM}$ Multipurpose; SB, $Adper^{TM}$ Single Bond 2; SE, $Clearfil^{(R)}$ SE Bond; AQ, AQ $Bond^{TM}$; TS, $Clearfil^{(R)}$ tri-S Bond). Except SM and SB, they have a simplified one- or two-step application protocols in compare with the dentin adhesives conventional three-step protocols. For the microtensile bond strength test, the labial surfaces of bovine incisors were used. Following exposure of dentin layer, according to their manufacturer's directions, each dentin adhesives were applied and composite resin blocks were constructed. The teeth were sectioned for specimen and tested microtensile bond strength. Also observed the fracture mode of interface. The obtained results were as follows : 1. The microtensile bond strength values ranged from 51.34 to 24.04 MPa on dentin(in decreasing order, SE, SM, SB, AQ and TS). 2. The highest microtensile bond strength was by SE and SM on bovine dentin, and the lowest by AQ and TS. 3. SM, SB and SE showed cohesive failures and adhesive failure but AQ, TS presented almost adhesive failures. In summary, microtensile bond strengths of single-step adhesives (AQ and TS) on bovine dentin were significantly lower than those of multi-step adhesives (SM, SB and SE) (p<0.05).

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Effect of Mushroom (Lentinus Tuber-Regium) Powder on the Bread Making Properties of Wheat Flour (버섯(Lentinus Tuber-Regium)분말 첨가가 제빵 특성에 미치는 영향)

  • Lee, Min-Jeong;Kyung, Kyu-Hang;Chang, Hak-Gil
    • Korean Journal of Food Science and Technology
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    • v.36 no.1
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    • pp.32-37
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    • 2004
  • Effects of mushroom powder on physicochemical characteristics of dough and bread-making properties were studied. In mixogram test, addition of 2-10% mushroom powder increased water absorption from 67,0 to 79%. Peak time, peak height, band width, and seven minute height of mushroom-wheat flour composite were similar to those of control. Sedimentation and P.K. values decreased with increasing amount of mushroom powder In amylogram test, no significant difference was observed in gelatinization temperature between control and mushroom powder-added dough. Peak viscosity increased with increasing amount of mushroom powder, Highest loaf volume was attained when 2 and 4% mushroom powders were added, whereas decreased above 6%. Bread weight and L value of crust increased with increasing amount of mushroom powder whereas 'a' value decreased. As the amount of mushroom powder increased, L value of crumb color decreased. No significant difference in springiness and adhesiveness was observed between control and mushroom-wheat composite flour bread whereas chewiness and gumminess, increased with increasing amount of mushroom powder, Hardness generally increased as the amount of mushroom powder increased. Mushroom powder caused bread staling at both storage temperatures ($4^{\circ}C\;and\;25^{\circ}C$). Although sensory value decreased with increasing mushroom powder, use of mushroom powder to replace up to 4% wheat flour is recommended in making bread.

Trends in Genetic Parameters with Age and Site for Early Implications of Genetic Improvement in Korean White Pine (잣나무의 유전적(遺傳的) 형질(形質) 개량(改良)의 조기검정(早期檢定)을 위한 수령(樹齡) 및 입지별(立地別) 생장(生長)과 유전모수(遺傳母數)의 특성(特性)에 관(關)한 연구(硏究))

  • Kim, Dae Eun;Chon, Sang Keun
    • Journal of Korean Society of Forest Science
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    • v.79 no.1
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    • pp.56-70
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    • 1990
  • Eighteen Korean white pine (P. koraiensis S. et Z.) families were tested in 3 different regions from age 5 to 9. Family and site were significant sources of variation for seedling survival and field growth, whereas the effects of family x site interaction ware relatively small as compared with the former sources of variation. Variance components estimated from the separate and combined sites indicated that the most variabilities were associated with individual trees within plot. Family ${\times}$ site interaction components as a percentage of family variance decreased sharply with age. Heritability estimates varied with testing site and tree age. Combined analyses, however, showed a moderate change in heritability with increasing tree ages, and demonstrated high and stable trends of estimates, particularly in family heritabilities of tree height ($h_F{^2}=0.789-0.798$). The gains estimated from combined analysis have expected maximum or near-maximum efficiencies at age 6 or 7. Given equal intensity of selection, mass selection showed the most efficient gains within and across the sites. However, for the differences between mass and combined selections are small, selection made on the combination of family and within-family would be more effective in improving genetic gains. Indirect selection method indicated that 5-and 6-years height were all good predictors of 9-year-old height with little loss of relative efficiency (less than 10%) as compared with direct family selection at age 9. Phenotypic and genetic correlations computed on the basis of family mean values of height and diameter have shown predominantly high, positive, and statistically significant (1% level) relationships between all tested pairs of traits, which indicates that family growth maintained statistically consistent trends with age. The best families are those that maintained a stable superiority overall sites and ages in growth performance, therefore, it can be suggested that early identification of superior families at age 9 is feasible at age 5 or 6 in Pinus koraiensis S. et Z.

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A STUDY ABOUT ALVEOLAR CREST BONE HEIGHT BEFORE AND AFTER ORTHODONTIC TREATMENT BY USING BITEWING FILM (교익사진을 이용한 교정치료 전후의 치조골 높이 변화에 관한 연구)

  • Hwang, Chung-Ju
    • The korean journal of orthodontics
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    • v.27 no.3 s.62
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    • pp.421-430
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    • 1997
  • Alveolar bone grows with development of tooth germs and roots; bone deposition occurs with tooth eruption. Bone components undergoes processes of resorption and deposition, and when the balance between them is disrupted, decrease in alveolar bone height or excessive bone deposition result. It has been hon that repositioning of teeth through orthodontic treatment can cause alveolar bone resorption which result in decreased alveolar bone height, and there have been many studies to evaluate such effects. X-ray films that could be replicated and standardized were chosen in clinical studies, and among them, bitewing films were used for objective evaluation of changes in alveolar bone level. Twenty subjects, 10 to 13-year- old (average 12.2) children with Cl I molar key, healthy oral condition, no congenital missing, no periodontal disease, and pre-and post-orthodontic bitewing films, were randomly selected for comparison of alveolar bone heights. Amounts of tooth and changes in alveolar bone heights were analyzed. The following results were obtained: 1. Amount of tooth movement in canine, premolar, and molar regions, changes in tooth axis, and changes in alveolar bone heights were measured, and the mean and median values were obtained. 2. When pre-and post-orthodontic alveolar bone levels were compared, larger changes were noticed in maxilla than mandible. 3. When mesio-distally compared, larger changes were observed in the distal sides of 3D3 and 4M3, mesial sides of 4M3 and 4D3, distal sides of 4D3 and 5M3, mesial sides of 5M3 and 5D3, md distal sides of 5D3 and 6M3. 4. When the amounts of tooth movements(TX, TY)and changes in tooth axis(A) were compared,34TX, 34TY, 34A of both sides in maxilla were greater, iud changes in alveolar bone level were greater than any other region.

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A Practical Method to Quantify Very Low Fluxes of Nitrous Oxide from a Rice Paddy (벼논에서 미량 아산화질소 플럭스의 정량을 위한 실용적 방법)

  • Okjung, Ju;Namgoo, Kang;Hoseup, Soh;Jung-Soo, Park
    • Korean Journal of Agricultural and Forest Meteorology
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    • v.24 no.4
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    • pp.285-294
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    • 2022
  • In order to accurately calculate greenhouse gas emissions in the agricultural field, Korea has been developing national-specific emission factors through direct measurement of gas fluxes using the closed-chamber method. In the rice paddy, only national-specific emission factors for methane (CH4) have been developed. It is thus necessary to develop those for nitrous oxide (N2O) affected by the application of nitrogen fertilizer. However, since the concentration of N2O emission from rice cultivation is very low, the QA/QC methods such as method detection and practical quantification limits are important. In this study, N2O emission from a rice paddy was evaluated affected by the amount of nitrogen fertilizer, by taking into account both method detection and practical quantification limits for N2O concentration. The N2O emission from a rice paddy soils affected by the nitrogen fertilizer application was estimated in the following order. The method detection limit (MDL) of N2O concentration was calculated at 95% confidence level based on the pooled standard deviation of concentration data sets using a standard gas with 98 nmol mol-1 N2O 10 times for 3 days. The practical quantification limit (PQL) of the N2O concentration is estimated by multiplying 10 to the pooled standard deviation. For the N2O flux data measured during the rice cultivation period in 2021, the MDL and PQL of N2O concentration were 18 nmol mol-1 and 87 nmol mol-1, respectively. The measured values above the PQL were merely about 12% of the total data. The cumulative N2O emission estimated based on the MDL and PQL was higher than the cumulative emission without nitrogen fertilizer application. This research would contribute to improving the reliability in quantification of the N2O flux data for accurate estimates of greenhouse gas emissions and uncertainties.

A Study of Serum Cytokines in the Patients with Chronic Hepatitis B Virus Infection (만성 B형 간염 바이러스 감염 환자에서 혈청 Cytokine에 관한 연구)

  • Kim, Byung-Ju;Ma, Jae-Sook;Hwang, Tai-Ju
    • Pediatric Gastroenterology, Hepatology & Nutrition
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    • v.1 no.1
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    • pp.90-99
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    • 1998
  • Purpose: The aim of this study was to clarify the serum cytokine pattern in patients with chronic HBV infection in terms of their clinical state. Methods: Intravenous blood samples were taken from 35 patients who were seropositive for HBsAg for at least 6 months and 7 healthy controls. Samples were initially tested for serum aminotransferases and serologic markers for hepatitis B virus by EIA. Serum levels of interleukin(IL)-2, tumor necrosis factor-alpha (TNF-${\alpha}$), interferon-gamma (IFN-${\gamma}$), IL-4, and IL-10 were measured by ELISA. Results: Among 35 patients, seropositive for HBeAg was 20 and for anti-HBe was 15. The histologic diagnosis of 19 patients underwent liver biopsy were chronic persistent hepatitis (CPH) in 10 and chronic active hepatitis (CAH) in 9. Serum IL-10 level in patients seropositive for HBeAg was significantly higher than that in patients seropositive for anti-HBe (p<0.05). All measured cytokine levels in patients with CAH were higher than those of patients with CPH. High values of all measured cytokines except IL-4 were seen in patients with AST and ALT > 100 U/L. High level of IL-4 was seen in patients with normal aminotransferase levels. Conclusion: These results were thought to indicate that anti-inflammatory Th2-like cytokine (IL-10) production in chronic HBV infection is related to circulating HBeAg rather than activity of hepatitis and that Th1 cytokines seem to be associated with the increasing activity of hepatitis.

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A Study on Fractionation and Characterization of Water-Soluble Natural Fe-Chelates From Garbage Compost and Activated Sewage Sludge (활성오니(活性汚泥) 및 진개퇴비중(塵芥堆肥中) 수용성(水溶性) 철(鐵) 킬레이트의 분리(分離)와 특성(特性)에 관(關)한 연구(硏究))

  • Park, Nae-Joung;Lindsay, W.L.
    • Applied Biological Chemistry
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    • v.18 no.4
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    • pp.194-202
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    • 1975
  • This study was conducted to study the properties of the water-soluble natural chelating agents from garbage compost and activated sewage sludge responsible for Fe chelation, which is closely associated with the effectiveness in correcting iron chlorosis in plant. The water-soluble fraction of these materials was fractionated by menas of Sephadex gel filtration and the fractions of Fe chehates were traced by radioactive $^{59}Fe$. The fractions were examined by ultraviolet and infrared. spectroscopy and stability constants for Fe. The water-soluble fraction from garbage compost was separated by Sephadex G-25 into approximately four fractions. Most of the added $^{59}Fe$ was associated with fraction I, which appeared at the void volume. Further fractionation by Sephadex G-50 indicated that the molecular weight of water-soluble chelating agents is in the approximate range of 5000 to 10,000. The water-soluble fraction from activated sewage sludge gave six fractions by Sephadex G-25. Most of the added $^{59}Fe$ was found in the fraction I,II, and III, The molecular weights of most chelating agents associated with $^{59}Fe$ appeared to be less than 5,000 and those of fraction I that appeared at the void volume was in the range of 5,000 to 1,000. Discrepancy between radio activity count and UV absorption indicated the heterogeneity of the fractions obtained by Sephadex gel filtration. Ultraviolet absorption spectra of all fractions separated by Sephadex G-25 and containing chelating agents showed no differences. Fraction IV and V of sewage extract showed absorption maxima and shifting similar to nucleic acid components suggesting the presence of decomposition products of nucleic acid. Similarity fraction VI contained phenolic type amino acid groups. Fraction I of compost extract contained most of the added $^{59}Fe$ and showed weak but extra definite absorption in the 1230, and $1270cm^{-1}$ region, suggesting that extra oxygen groups in polyphenolic structure were probably involved in Fe chelation. In sewage extract, fraction I,II, and III in which most of the $^{59}Fe$ was found, showed strong definite polypeptide absorption in the region of $1540cm^{-1}$ due to NH deformation and C-N stretching of amide groups in the peptidebond. These extra functional groups in fraction I, II, and III appeared to be associated with Fe chelation. The other fractions, not associated with $^{59}Fe$, still have carboxyl and hydroxyl groups, suggesting that these functional groups in these water extracts may not independently form the Fe chelates. Precipitation of ferric hydroxide precluded measuring the stability constants for Fe-chelates. However, the formation constants for Zn chelates as log K values for compost extract and sewage extract at pH 4.0 from which the strength of chelation with Fe could be presumed, were 8.23, and 9.75, respectively, indicating strong complexation with metals. The chelating capacity of compost extract containing 6.5 g organic matter per liter was 0.82 mM, and that of sewage extract containing 5.3 g per liter was 0. 64 mM.

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