• Microbiology and Biotechnology Letters
• /
• v.40 no.4
• /
• pp.348-355
• /
• 2012

In the present study, we investigated the overexpression and characterization of bovine pancreatic (bp)- DNase I in Saccharomyces cerevisiae and Pichia pastoris. The bp-DNase I gene was fused in frame with the GAL10 promoter, $MF{\alpha}$, and GAL7 terminator sequences, resulting in the plasmid, pGAL-$MF{\alpha}$-DNaseI (6.4 kb). Also, the bp-DNase I gene was fused in frame with the AOX1 promoter, $MF{\alpha}$, and AOX1 terminator sequences, resulting in the plasmid, pPEXI (8.8 kb). The recombinant plasmids, pGAL-$MF{\alpha}$-DNaseI and pPEXI were introduced into S. cerevisiae and P. pastoris host cells, respectively. When the transformed yeast cells were cultured at $30^{\circ}C$ for 48 h in galactose or methanol medium, bp-DNase I was overexpressed and the most of activity was found in the extracellular fraction. P. pastoris transformant activity showed 45.5 unit/mL in the culture medium at 48 h cultivation, whereas S. cerevisiae transformant revealed 37.7 unit/mL in the extracellular fraction at 48 h cultivation. The enzymatic characteristics, such as DNA cleavage and half life were investigated. Treatment of the recombinant DNase I from P. pastoris induced degradation of the calf thymus DNA within 1 minute, and this DNA degradation rate was higher than that of commercial bp-DNase I (SIGMA) and the recombinant DNase I from S. cerevisiae.

• Korean Journal of Microbiology
• /
• v.24 no.4
• /
• pp.341-351
• /
• 1986

R-plasmid(pSBK203, 2.5Mdal) conferring chloramphenicol resistance was isolated from mutiple antibiotic resistant Staphylococcus aureus D-H-1. Bacillus subtilis BD170 was transformed by this plasmid and restriction enzyme clevage sites of this plasmid were mapped for the cloning of chloramphenicol resistance gene. Taq I partial digested fragment of pSBK203(1.3kb) inserted into Cla I site of pBD9 appears to have both regulatory region for induction and structural gene for chloramphenicol resistance whereas Rsa I fragment (1.3kb, both ends are staggered away 0.1Kb from those of Taq I fragment) inserted into Sca I site of pBR322 showed constitutive expression in E. coli. Hinf I, Taq I, and Bgl II restriction enzyme recognition sites are found in both Rsa I fragment and Taq I fragment. Among these, Bgl II recognition site was associated with chloramphenicol resistance.

• Korean Journal of Soil Science and Fertilizer
• /
• v.37 no.4
• /
• pp.220-227
• /
• 2004

Phosphorus desorption study is essential to understanding P behavior in agricultural and environmental soils because phosphorus is considered as two different aspects, a plant nutrient versus an environmental contaminant. This study was conducted to determine soil P buffering power related to P desorption quantity intensity (Q/I) parameters, $Q_{max}$(an index of P release capacity) and $l_0$(an index of the intensity factor), and to investigate the characteristics of relationship between the P desorption Q/I parameters and the soil properties. Soil samples were prepared with treatments of 0 and $100mg\;P\;kg^{-1}$ applied as $KH_2PO_4$ solution. The P desorption Q/I curves were obtained by a procedure using anion exchange resin beads and described by an empirical equation ($Q=aI^{-1}+bln(I+1)+c$). The P desorption Q/I curves for the high available P (${\g}20mg\;kg^{-1}$ of Olsen P) soils were characteristic concave trends with or without soil P enrichment, whereas for the low available P (${\lt}20mg\;kg^{-1}$ of Olsen P) soils, the anticipated Q/I concave curves could not be obtained without a proper amount of P addition. When the soils were enriched in phosphates, the values of desorbed solid phase labile P and solution P, such as $Q_{max}$ and $I_0$ respectively, were increased, but the ratio of $Q_{max}$ versus $I_0$ was decreased. Thus, the slope of desorption Q/I curve represented as phosphorus buffering power, $|BP_0|$, is decreased. The $|BP_0|$ values of the high available P soils ranged between 48 and $61L\;kg^{-1}$ in the P untreated samples and between 18 and $44L\;kg^{-1}$ in the P enriched samples. Overall $|BP_0|$ values of both low and high available P soils treated with $l00mg\;P\;kg^{-1}$ ranged between 14 and $79L\;kg^{-1}$. The $Q_{max}$, values ranged between 71.4 and $173.1mg\;P\;kg^{-1}$, and the lo values ranged between 0.98 and $3.82mg\;P\;L^{-1}$ in the P enriched soils. The $Q_{max}$ and $I_0$ values that control the P buffering power may be not specifically related to a specific soil property, but those values were complicatedly related to soil pH, clay content, soil organic matter content, and lime. Also, phosphorus release activity, however, markedly depended on the desorbability of the applied P as well as the native labile P.

• Journal of applied mathematics & informatics
• /
• v.26 no.3_4
• /
• pp.457-469
• /
• 2008

In this paper, we study the existence of positive solutions for the following nonlinear m-point boundary value problem with p-Laplacian: $\{{{{(\phi_p(u'))'\;+\;f(t,u(t))=0, \;0<t<1,} \atop u'(0)={\sum}{^{m-2}_{i=1}}\;a_iu'(\xi_i),} \atop u(1)={\sum}{^k_{i=1}}\;b_iu(\xi_i)\;-\;{\sum}{^s_{i=k+1}}\;b_iu(\xi_i)\;-\;{\sum}{^{m-2}_{i=s+1}}\;b_iu'(xi_i),}$ where ${\phi}_p(s)$ is p-Laplacian operator, i.e., ${\phi}_p(s)=\mid s\mid^{p-2}s$, p>1, ${\phi}_q\;=\;({\phi}_p)^{-1}$, $\frac{1}{p}+\frac{1}{q}=1$, $1\;{\leq}\;k\;{\leq}\;s\;{\leq}m\;-\;2$, $b_i\;{\in}\;(0,+{\infty})$ with $0\;<\;{\sum}{^k_{k=1}}\;b_i\;-\;{\sum}{^s_{i=k+1}}\;b_i\;<\;1$, $0\;<\;{\sum}{^{m-2}_{i=1}}\la_i\;<\;1$, $0\;<\;{\xi}_1\;<\;{\xi}_2\;<\;{\cdots}\;<\;{\xi}_{m-2}\;<\;1$, $f\;{\in}\;C([0,\;1]\;{\times}\;[0,\;+{\infty}),\;[0,\;+{\infty}))$. We show that there exists one or two positive solutions by using fixed-point theorem for operator on a cone. The conclusions in this paper essentially extend and improve the known results.

• East Asian mathematical journal
• /
• v.18 no.2
• /
• pp.271-282
• /
• 2002

Let R be a ring with an endomorphism $\sigma$ and a derivation $\delta$. An ideal I of R is ($\sigma,\;\delta$)-ideal of R if $\sigma(I){\subseteq}I$ and $\delta(I){\subseteq}I$. An ideal P of R is a ($\sigma,\;\delta$)-prime ideal of R if P(${\neq}R$) is a ($\sigma,\;\delta$)-ideal and for ($\sigma,\;\delta$)-ideals I and J of R, $IJ{\subseteq}P$ implies that $I{\subseteq}P$ or $J{\subseteq}P$. An ideal Q of R is ($\sigma,\;\delta$)-semiprime ideal of R if Q is a ($\sigma,\;\delta$)-ideal and for ($\sigma,\;\delta$)-ideal I of R, $I^2{\subseteq}Q$ implies that $I{\subseteq}Q$. The ($\sigma,\;\delta$)-prime radical (resp. prime radical) is defined by the intersection of all ($\sigma,\;\delta$)-prime ideals (resp. prime ideals) of R and is denoted by $P_{(\sigma,\delta)}(R)$(resp. P(R)). In this paper, the following results are obtained: (1) $P_{(\sigma,\delta)}(R)$ is the smallest ($\sigma,\;\delta$)-semiprime ideal of R; (2) For every extended endomorphism $\bar{\sigma}$ of $\sigma$, the $\bar{\sigma}$-prime radical of an Ore extension $P(R[x;\sigma,\delta])$ is equal to $P_{\sigma,\delta}(R)[x;\sigma,\delta]$.

• Honam Mathematical Journal
• /
• v.33 no.2
• /
• pp.153-161
• /
• 2011

Greg([Greg]) considered that $$N_k= \sum\limits_{i=1}^k(-1)^{i+1}P_{i,k}(p)N_1^i$$ where the $P_{i,k}$'s were polynomials with positive integer coefficients. In this paper, we will give the equations for $\sum\limits{P_{i,k}$ modulo 3. Using this, if we send a information for elliptic curve to sender, we can make a new checksum method for Manchester coding in IEEE 802.3 or IEEE 802.4.

• KSBB Journal
• /
• v.18 no.4
• /
• pp.329-334
• /
• 2003

The present study was aimed at investigating the regulatory mechanism in tissue-specific expression of insulin-like growth factor-I (IGF-I) gene. The expression of IGF-I gene was determined by a solution hybridization/RNase protection assay using total RNA prepared from rat liver or brain of various ages. The levels of IGF-I transcripts were increased in liver gradually after birth, but decreased in brain. By using an oligonucleotide (FRE) corresponding to the C/EBP binding site of the rat IGF-I exon 1, multiple forms of C/EBP${\alpha}$ and C/EBP${\beta}$ proteins, which have DNA-binding activity, were detected in the rat liver or brain. Western immunoblot and southwestern analyses show that p42$\^$C/EBP${\alpha}$/, p38$\^$C/EBP${\alpha}$/, p35$\^$C/EBP${\alpha}$/, p38$\^$C/EBP${\beta}$/, and p35$\^$C/EBP${\beta}$ form specific complexes with the IGF-I exon 1 oligonucleotide in liver nuclear extract and that p42$\^$C/EBP${\alpha}$/ and p38$\^$C/EBP${\beta}$/ form complexes in brain. These data suggest that the formation of FRE-C/EBP isoform complexes may play important roles in the tissue-specific regulation of IGF-I gene expression.

• Korean journal of applied entomology
• /
• v.32 no.1
• /
• pp.51-60
• /
• 1993

The polyhedrin gene-containing DNA fragment of Hyphantria cunea nuclear polyhedrosis virus (HcNPV) was localized by southern hybridization with Autographa california CPA EcoRI-I fragment (7.3 kb), Bombyx mori NPV PatI-F fragment (7 kb) and synthetic oligonucleotide(30-mer) as probes. the PstI-L(5.3 kb) fragment of HcNPV was cloned to E. coli and the plasmid of the fragment was named as pHcP-L(8.0 kb). The pHcP-L was physically mapped and subcloned to E. coli as pHcP-L1(4.7 kb), pHcP-L2(7.1 kb), pHcP-L3(5.3 kb), pHcP-L4(4.2 kb) and pHcP-L5(4.5 kb).

• Korean Journal of Food Science and Technology
• /
• v.16 no.4
• /
• pp.392-397
• /
• 1984

These experiments were conducted to investigate general enzymatic characteristics of two forms(glucoamylase I and glucoamylase II) of the purified glucoamylase produced by Rhizopus oryzae. Molecular weights of glucoamylase I and glucoamylase II estimated by Sephadex G-100gel filtration, were approximately 101,000 and 115,000, respectively, and those estimated by SDS-polyacrylamide gel electrophoresis being 120,000 and 127,000, respectively. Isoelectric points of the above enzyme were pH 7.25 and pH 7.75. The optimum temperature was $50^{\circ}C$ and the enzyme was stable below $45^{\circ}C$. Optimum pH of both glucoamylase I and glucoamylase II was about pH 5.0. The stable pH range of them were pH 3.5-8.0 and 4.5-8.0, respectively. Michaelis constants of glucoamylase I and glucoamylase II toward souluble starch were 4.545 mg/ml and 5.560 mg/ml, respectively. $Hg^{++}$, $Pb^{++}$, p-CMB and IAA were inhibitors of glucoamylase I and $Hg^{++}$, $Mn^{++}$, p-CMB and IAA were inhibitors of glucoamylase II.

• Korean Journal of Community Nutrition
• /
• v.18 no.1
• /
• pp.55-64
• /
• 2013

This study was conducted to investigate the effect of nutrition education on improving dietary attitudes, food habits and Food Frequency of 32 female shift workers by administering questionnaires. Dietary nutrient consumption data were obtained from the female shift workers by using 1 day 24-hr recall. The results were as follows: Score on 'Concerns about health (p < 0.05)' was significantly higher after-training. Dietary attitude (32.3 vs. 34.9, p < 0.01) and food habits (60.2 vs. 67.1, p < 0.01) were significantly higher after the training. Scores on 'Eating a lot of food that I want to eat is more important than nutrition (p < 0.01)', 'I have a tendency to use instant foods when I am busy (p < 0.01)', 'I am interested in information on nutrition and health (p < 0.01)' were significantly higher after the training. Scores on 'I have three meals a day (p < 0.001)', 'I have breakfast regularly (p < 0.01)', 'I take vegetables other than kimchi at every meal (p < 0.01)', 'I drink milk every day (p < 0.001)', 'I eat fruits every day (p < 0.01)', and 'I apply nutrition knowledge to daily life (p < 0.001)' were significantly higher after the training. Protein (p < 0.05), fiber (p < 0.05), vitamin C (p < 0.01), and calcium (p < 0.001) intakes were significantly higher after the training. These results showed that nutrition education had improved the dietary attitudes, food habits, and nutrient intakes. Therefore, we conclude that providing more systematic and appropriate nutrition education to shift workers during their tenure of office period is likely to improve dietary attitudes, food habits, and nutrient intakes.