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Biochemical toxicity of Corexit 9500 dispersant on the gills, liver and kidney of juvenile Clarias gariepinus

  • Ugbomeh, A.P.;Bob-manuel, K.N.O.;Green, A.;Taylorharry, O.
    • Fisheries and Aquatic Sciences
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    • v.22 no.7
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    • pp.15.1-15.8
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    • 2019
  • Corexit 9500 is a dispersant commercially available in Nigeria that is used to change the inherent chemical and physical properties of oil, thereby changing the oil's transport and fate with potential effects on the environment. The aim of this study was to assess the biochemical (enzymes and electrolyte) toxicity of Corexit 9500 dispersant on the gills, liver and kidney of juveniles of Clarias gariepinus after exposure for 21 days. One hundred sixty fish were used without gender consideration. Range-finding tests were conducted over a 96-h period after acclimatisation of the test organisms in the laboratory. The test organisms (10/treatment) were exposed to Corexit 9500 in the following concentrations-0.00, 0.0125, 0.025 and 0.05 ml/l in triplicate. Twenty-one days later, fish was dissected. 0.5 g from each of the following organs-gills, liver and kidney tissues-was removed, homogenised and tested for enzymes [superoxide dismutase (SOD), catalase (CAT), alanine aminotransferase (ALT), aspartate transaminase (AST), alkaline phosphatase (ALP)], urea, creatinine and electrolytes (sodium ($Na^+$), potassium ($K^+$), chloride ($Cl^-$), bicarbonate ($HCO_3{^-}$)) following standard methods. In the gills, SOD and ALT to AST ratio were significantly lower than in control while the creatinine was significantly higher in the toxicant. In the kidney, creatinine was significantly higher in fish exposed to the toxicant. In the liver, ALP increased in the toxicant while urea was decreased. The mean electrolyte concentrations ($Na^+$, $K^+$, $Cl^-$ and $HCO_3{^-}$) increased significantly in the concentration of the toxicant (P < 0.05). The alterations observed in the activities of these electrolytes and enzymes indicated that Corexit 9500 interfered with transamination and metabolic functions of the fish.

Factors associate with Musculoskeletal Symptoms in Small and Medium Sized Hospital Nurses (중소병원간호사의 근골격계증상 영향 요인)

  • Jung, Ji Soo;Eun, Young
    • Journal of muscle and joint health
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    • v.24 no.3
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    • pp.159-170
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    • 2017
  • Purpose: The purposes of this study were to identify musculoskeletal symptoms and to investigate factors associated with musculoskeletal symptoms in small and medium sized hospital nurses. Methods: The data were collected from 206 nurses working in Jeonnam. We used self administration questionnaires of KOSHA GUIDE H-9-2012 for musculoskeletal symptoms, PWI-SF for psychosocial stress, KOSS for job stress, and HPLP-II for health behaviors. The collected data were analyzed using the $x^2$, t-test, and logistic regression analysis in SPSS Ver. 23.0. Results: Sixty-six percent of the subjects had musculoskeletal symptoms. The shoulder had the highest symptom at 38.3%, followed by lower back at 32.5%, and neck at 29.1%. The mean and standard deviation score of psychosocial stress were $24.06{\pm}6.01$, those of job stress were $2.38{\pm}0.23$, and those of health behaviors were $2.14{\pm}0.39$. The items that were significantly different according to musculoskeletal symptoms were career ($x^2=6.67$, p<.036), one week overtime ($x^2=7.27$, p<.026), subjective health status ($x^2=4.29$, p<.038), and psychosocial stress ($x^2=7.99$, p<.010). In logistic regression analysis, career, and psychosocial stress were found to affect musculoskeltal symptoms. Conclusion: It is necessary to conduct preventive intervention to reduce musculoskeletal symptoms according to career and psychosocial stress for small and medium sized hospital nurses.

A study on the fixation of heavy metals with modified soils in the landfill liner (개량혼합토를 이용한 폐기물 매립지 차수층의 중금속 고정능력에 관한 연구)

  • 노회정;이재영
    • Journal of Soil and Groundwater Environment
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    • v.7 no.2
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    • pp.63-71
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    • 2002
  • The authors selected the modified soil method, and then performed the geotechnical and environmental laboratory test, and evaluated whether the modified soil liner could be accepted as a barrier layer in landfill. Unlike the results of the natural soil(CL), those of the hydraulic conductivity test of stabilized soil met the standard value. According to these results, the optimal mixing ratio of a mixture(cement : bentonite : stabilizing agent) was 90 : 60 : 1 with mass ratio(kg) for 1㎥ with soil, and it was possible to use poor quality bentonite. B\circled2 because of a little difference from results with high quality bentonite. B\circled1. The Cation Exchange Capacity(CEC) of the modified soil was increased about 1.5 times compared with the natural soil; however. the change of CEC with a sort of additives was not detected. In order to observe the change of the chemical components and crystal structures, the natural and the modified soils with the sorts of additives were measured by the XRF(X-Ray Flourescence Spectrometer) and SEM, but there was no significant change. The artificial leachate with the heavy meals ($Pb^{2+}$ , $Cu^{2+}$, $Cd^{2+}$ Zn$^{2+}$ 100mg/L) was passed through the natural soil and modified soils in columns. In the natural soil, Cd$^{2+}$ and $Zn^{2+}$ were identified, simultaneously the pH of outflow was lower, and then came to the breakthrough point. The removal efficiency of the natural soil was showed in order of following : $Pb^{2+}$$Cu^{2+}$ > $Zn^{2+}$ > $Cd^{2+}$ On the other hand, modified soils were not showed the breakthrough condition like the result of the natural soil. The modified soil with the lower quality bentonite, B\circled2(column3) was more stable with respect to chemical attack than that with the higher bentonite, B\circled1(column2) because the change range of outflow pH in columns was less than that of outflow pH in column2. In addition, the case of adding the stabilizing agent(column4) was markedly showed the phenomena.ena.

Development of Marine Ecotoxicological Standard Methods for Ulva Sporulation Test (파래의 포자형성률을 이용한 해양생태독성시험 방법에 관한 연구)

  • Han, Tae-Jun;Han, Young-Seok;Park, Gyung-Soo;Lee, Seung-Min
    • The Sea:JOURNAL OF THE KOREAN SOCIETY OF OCEANOGRAPHY
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    • v.13 no.2
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    • pp.121-128
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    • 2008
  • As an aquatic ecotoxicity test method, a bioassay using the inhibition of sporualtion of the green macroalga, Ulva pertusa, has been developed. Optimal test conditions determined for photon irradiance, pH, salinity and temperature were $100\;{\mu}mol{\cdot}m^{-2}{\cdot}s^{-1}$, $7{\sim}9$, $25{\sim}35\;psu$ and $15{\sim}20^{\circ}C$, respectively. The validity of the test endpoint was evaluated by assessing the toxicity of four metals (Cd, Cu, Pb, Zn) and elutriates of sewage or waste sludge collected from 9 different locations. When the metals were assayed, the $EC_{50}$ values indicated the following toxicity rankings: Cu ($0.062\;mg{\cdot}L^{-1}$) > Cd ($0.208\;mg{\cdot}L^{-1}$) > Pb ($0.718\;mg{\cdot}L^{-1}$) > Zn ($0.776\;mg{\cdot}L^{-1}$). When compared with other commonly used bioassays of metal pollution listed on US ECOTOX database, the sporualtion test proved to be the most sensitive. Ulva sporulation was significantly inhibited in all elutriates with the greatest and least effects observed in elutriates of sludge from industrial waste ($EC_{50}=6.78%$) and filtration bed ($EC_{50}=15.0%$), respectively. The results of the Spearman rank correlation analysis for $EC_{50}$ data versus the concentrations of toxicants in the sludge presented a significant correlation between toxicity and four heavy metals(Cd, Cu, Pb, Zn). The method described here is sensitive to toxicants, simple to use, easy to interpret and economical. It is also easy to procure samples and maintain cultures. The present method would therefore probably make a useful assessment of aquatic toxicity of a wide range of toxicants. In addition, the genus Ulva has a wide geographical distribution and species have similar reproductive processes, so the test method would have a potential application worldwide.

INVESTIGATION OF THE EFFECT OF AN ANTIBIOTIC "P" ON POTATOES ("감자에 대한 항생제(抗生劑) 피마리신의 통계적(統計的) 효과(效果) 분석(分析)")

  • Kim, Jong-Hoon
    • Journal of Korean Society for Quality Management
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    • v.5 no.2
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    • pp.59-120
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    • 1977
  • An antibiotic 'P', which is one of the products of the Gist Brocades N. V. is being tested by its research department as fungicide on seed-potatoes. For this testing they designed experiments, with two control groups, one competitor's product, eight formulations of the antibiotic to be tested in different concentrations and one mercury treatment which can not be used in practice. The treated potatoes were planted in three different regions, where bifferent conditions prevail. After several months the harvested potatoes are divided in groups according to their diameter, potato illness is analysed and counted. These data were summarised in percentage and given to us for Analysis. We approached and analysed the data by following methods: a. Computation of the mean and standard deviation of the percenage of good results in each size group and treatment. b. Computation of the experimental errors by substraction of each treatment mean from observed data. c. Description of the frequency table, plotting of a histogram and a normal curve on same graph to check normality. d. Test of normality paper and chi-sqeare test to check the goodness of fit to a normal curve. e. Test for homogeneity of variance in each treatment with the Cochran's test and Hartley's test. f. Analysis of Variance for testing the means by one way classifications. g. Drawing of graphs with upper and lower confidence limits to show the effect of different treatments. h. T-test and F-test to two Control mean and variance for making one control of Dunnett's test. i. Dunnett's Test and calculations for numerical comarision of different treatments wth one control. In region R, where the potatoes were planted, it was this year very dry and rather bad conditions to grow potatoes prevailed during the experimental period. The results of this investigation show us that treatment No.2, 3 and 4 are significantly different from other treatments and control groups (none treated, just like natural state). Treatment no.2 is the useless mercury formulation. So only No. 3 and 4, which have high concentrations of antibiotic 'P', gave a good effect to the potatoes. As well as the competitors product, middle and low concentrated formulations are not significantly different from control gro-ups of every size. In region w, where the potatoes got the same treatments as in region R, prevailed better weather conditions and was enough water obtainable from the lake. The results in this region showed that treatment No. 2, 3, 4, and 5 are Significantly different from other treatments and the control groups. Again No.2 is the mercury treatmentin this investigation. Not only high concentrated formulation of antibiotic 'P', but also the competitor's poroduct gave good results. But, the effect of 'P', was better than the competitors porduct. In region G, where the potatoes got the same treatments as in the regions R and w. and the climate conditions were equal to region R, the results showed that most of the treatments are not significantly different from the control groups. Only treatment no. 3 was a little bit different from the others. but not Significantly different. It seems to us that the difference between the results in the three regions was caused by certain conditions like, the nature of the soil the degres of moisture and hours of sunshine, but we are not sure of that. As a conclusion, we can say that antibiotic 'P' has a good effect on potatoes, but in most investigations a rather high concentration of 'P' was required in formulations.

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Retardation Effect and Mobility of a Heavy Metal in a Sandy Soil (사질토양에서의 중금속의 지연효과와 이동성)

  • Kim, Dong-Ju;Baek, Doo-Sung
    • Journal of the Korean Society of Groundwater Environment
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    • v.5 no.3
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    • pp.155-161
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    • 1998
  • Retardation effect of heavy metals in soils caused by adsorption onto the surfaces of solids particles is well known phenomenon. In this study, we investigated the retardation effect on the mobility of a Zn in a sandy soil by conducting batch and column tests. The column test consisted of monitoring the concentrations of effluent versus time known as a breakthrough curve (BTC). We used NaCl and ZnCl$_2$ solutions with the concentration of 10 g/L as a tracer, and injected them respectively into the inlet boundary of the soil sample as a square pulse type, and monitored the effluent concentrations at the exit boundary under a steady state condition using an EC-meter and ICP-AES. The batch test was conducted based on the standard procedure of equilibrating fine fractions collected from the soil with various initial ZnCl$_2$ concentrations, and analysis of Zn ions in the equilibrated solutions using ICP-AES. The results of column test showed that i) the peak concentration of ZnCl$_2$analyzed by ICP was far less than that of either NaCl or bulk electrical conductivity and ⅱ) travel times of peak concentrations for two tracers were more less identical. The relatively low concentration of Zn can be explained by ion exchange between Zn and other cations, and possible precipitation of Zn in the form of Zn(OH)$_2$due to high pH range (7.0∼7.9) of the effluent. The identical result of travel times of peak concentrations indicates that the retardation effect is not present in the soil. The only way to describe the prominent decrease of Zn ion was to introduce decay or sink coefficient in the CDE model to account for irreversible decrease of Zn ions in the aqueous phase.

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A Study on the Effects of Ankle Pump Exercise in Reducing Lower Limbs Edema and Pain of Operating Room Nurses (통나무 도구를 이용한 발목 펌프 운동이 수술실 간호사의 하지 부종 및 하지 통증에 미치는 효과)

  • Bae, Hye-Jin;Kim, Joo Hyun
    • Journal of Korean Biological Nursing Science
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    • v.16 no.3
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    • pp.235-243
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    • 2014
  • Purpose: The purpose of this study was to identify the effects of Ankle Pump Exercise on lower limb edema and pain of operating room nurses. Methods: This research was arranged on the basis of the nonequivalent control group pre-post test design. The participants were 41 nurses working in the operating room in 'S' hospital in Seoul and 'H' hospital in Guri City, Korea. Data were collected from August 20 to September 30, 2012. Edema of the lower extremities was measured in terms of the girths of ankles and calves. Pain of the lower extremities was measured in terms of VAS (Visual Analogue Scale) for pain. The data were analyzed by frequency, percentage mean, and standard deviation, using the chi2(p) and t-test, repeated measures ANOVA, and a paired t-test. The SPSS Win 20.0 program was used for analysis. Results: Hypothesis 1 - "There are significant differences in lower extremity edema between the experimental group and the control group." - was partially supported. Hypothesis 2 - "There are significant differences in lower extremity pain between the experimental group and the control group." - was supported. Conclusion: The results of this study demonstrate positive practical effects of the Ankle Pump Exercise in reducing the circumference of both ankles, reducing the circumferential edema of both calves, and alleviating the pain of nurses in the operating room. Based on these findings, application of the Ankle Pump Exercise can be considered as an effective method to prevent nurses' health problems in the lower extremities that can be induced by their work during surgical operations.

Fabrication of [320×256]-FPA Infrared Thermographic Module Based on [InAs/GaSb] Strained-Layer Superlattice ([InAs/GaSb] 응력 초격자에 기초한 [320×256]-FPA 적외선 열영상 모듈 제작)

  • Lee, S.J.;Noh, S.K.;Bae, S.H.;Jung, H.
    • Journal of the Korean Vacuum Society
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    • v.20 no.1
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    • pp.22-29
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    • 2011
  • An infrared thermographic imaging module of [$320{\times}256$] focal-plane array (FPA) based on [InAs/GaSb] strained-layer superlattice (SLS) was fabricated, and its images were demonstrated. The p-i-n device consisted of an active layer (i) of 300-period [13/7]-ML [InAs/GaSb]-SLS and a pair of p/n-electrodes of (60/115)-period [InAs:(Be/Si)/GaSb]-SLS. FTIR photoresponse spectra taken from a test device revealed that the peak wavelength (${\lambda}_p$) and the cutoff wavelength (${\lambda}_{co}$) were approximately $3.1/2.7{\mu}m$ and $3.8{\mu}m$, respectively, and it was confirmed that the device was operated up to a temperature of 180 K. The $30/24-{\mu}m$ design rule was applied to single pixel pitch/mesa, and a standard photolithography was introduced for [$320{\times}256$]-FPA fabrication. An FPA-ROIC thermographic module was accomplished by using a $18/10-{\mu}m$ In-bump/UBM process and a flip-chip bonding technique, and the thermographic image was demonstrated by utilizing a mid-infrared camera and an image processor.

Pregnant Women's Attitude and Satisfaction for Sexuality (임부의 성 태도와 성 만족과의 관계)

  • Lee, Yeong-Pyo;Kim, Sin-Jeong;Jeong, Geum-Hui
    • Journal of Korean Academy of Nursing
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    • v.30 no.5
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    • pp.1292-1302
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    • 2000
  • The relationship between the attitude and satisfaction for sexuality of pregnant women was observed to provide rationales of nursing intervention to help promote healthy sexual lifestyles. Questionnaires were collected from 211 obstetric outpatients of H university hospital in C city, Korea from February to May 1999. The research tools were D.S.F.I (Derogatis Sexual Function Inventory in Korean, Chronbach's $\alpha=$ 0.710) and Sexuality Satisfaction Method (Kim, 1997, Chronbach's $\alpha$ =0.864). Data was analyzed for frequency, mean, standard deviation, Pearson correlation, t-test and ANOVA by Windows SAS. The results of this study were as follows: Mean age of the subjects were 29.8; the average score of attitude to sex was moderate (27.60), and that of satisfaction sex was high (54.11); the positive relationship was shown between attitude and satisfaction for sexuality (r=0.51, p=.000); the higher educational and income levels of pregnant women and their husbands, were the better the attitude and satisfaction for was; professional women had better attitude than housewives. According to the results, it is suggested that the study is necessary to develop an effective nursing intervention related with the sexuality of pregnant women.

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Attitudes of Physicians and Nurses toward with Disabled Person (의사와 간호사의 장애인에 대한 태도)

  • Jung, Hyun-Kyeong;Kim, Myung-Hee
    • The Korean Journal of Rehabilitation Nursing
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    • v.7 no.2
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    • pp.213-219
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    • 2004
  • Purpose: This study was designed to explore the attitudes of physicians and nurses. Method: Attitudes were measured by using the korean version of the SADP(Scale of Attitudes toward the Disabled Persons : Choi, G. H., & Lam, C. S., 2000). A korean version SADP consist of 24-item, six-point Likert-type scale(the points are -3, -2, -1, +1, +2, +3, where -3 is disagree very much and +3 is Agree very much), measuring general attitudes toward people with disabilities. The sample included 90 physicians, 114 nurses at a university hospital in Busan. The data were collected using questionnaires, and were analyzed using frequency, percentage, mean score, standard deviation, t-test and one-way ANOVA. Result: When studying the physicians group by itself, SADP scores tended to increase with women physicians, old age, married, higher educational level, more years of experience, medical unit. When studying the nurses group by itself, SADP scores tended to increase with the age of thirty, single, educational level, more years of experience, surgical unit. But not to a statistically significant degree. Conclusion: Findings suggest that the nurses had significantly higher score than the physicians(t=-4.63, p<.001). When studying the physicians group and the nurses group by itself, not to a statistically significant degree.

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