• Proceedings of the Korea Society of Poultry Science Conference
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• 2002.11a
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• pp.103-105
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• 2002

This study was conducted to determine how broiler performance with the level of crude protein(23 CP%, 21 CP% and 19 CP%) can be changed and affected, adding Alum(Al$_{2}$(SO$_{4}$)$_{3}$ㆍ14H$_{2}$O) and no Alum in litter(two ALUM levels and 3 Protein levels with 2$\times$3 factorial design). Alum was added as a top dressing to the litter at a rate of 200g ALUM / kg of rice bran. With the exception of the protein levels for feed ：gain(P〈 0.05), there were no significant differences in feed intake, weight gain and feed ：gain of chicks. For ammonia gas emission, both the main effects of Alum and protein(P〈 0.01 and 0.05) at 3weeks and interaction between Alum and protein(P〈 0.05), the main effect of Alum(P〈 0.01) at 6weeks did affected them. In litter excluded the main effects of Alum(P〈 0.05), there was a significant difference among N contents ranged from pH to the rate of C： Organic-N(P〈 0.01).

• Korean Journal of Microbiology
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• v.33 no.3
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• pp.175-180
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• 1997

In an artificial pH-gradient hatch culture system, the author analyzed the effects of acidification on the species composition of heterotrophic bacteria. As the result of this study, it was found that the numbers of total bacteria were not affected by acidification and that the population size of heterotrophic bacteria decreased as pH became lower. The heterotrophic bacteria isolated from all of the pH gradient were 12 genera and 22 species. and among them, gram negative and gram positive bacteria were 64% and 36%, respectivcly. As pH decreased, the distribution rate of gram negative bacteria increased while that of gram positive bacteria decreased. Regarding to distrihution rate of genuses in each pH gradient, 13 genuses appeared at pH 7 while only 5 genuses appeared at pH 3. which means that the diversity of genera decrease as pH decreased. As a result of cluster analysis, diversity indices 01 species had ranges from 1.13 to 2.37, and decreased as pH decreased. In order to evaluate the diversity of different size samples, we analyzed the expected number of species appearance according to pH by rarefaction method. The statistical significance of species diversity was verified by the fact that the number decreased at lower pH.

• Journal of Korean Society of Water and Wastewater
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• v.27 no.6
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• pp.703-709
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• 2013

Among many microalgae cultivation types, heterotrophic culture with low cost carbon sources and energy saving culture method is crucial. A result of estimating the effects of pH on wastewater treatment using heterotrophic growing microalgae Chlorella sorokiniana shows that there was no difference in microalgae growth amount and nitrogen, phosphorus removal rate by wide range of pH(5 ~ 9). From pH 5 to 9, total nitrogen, phosphorous and glucose removal rates were 10.5 mg-N/L/d, 2 mg-P/L/d, 800 ~ 1000 mg/L respectively. This study reveals that C. sorokiniana cannot metabolite glycerol heterotrophically, however, glucose and acetate were proper carbon sources for growth and T-N, T-P and TOC removal. This research highlights the potential of heterotrophic microalgal growth with wastewater treatment plant with wide range of pH and carbon sources.

• Archives of Pharmacal Research
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• v.20 no.6
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• pp.643-646
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• 1997

Thermal stability of a red pigment, carthamin, frm Carthamus tinctorius was investigated to explore possible applications as natural color additives for foods, cosmetics, and nutraceuticals. Degree of degradation reactions of carthamin at acidic, neutral and alkaline conditions were determined with UV/V is spectral measurements. Decomposition half lives of carthamin at 25.deg. C were 4.0 h, 5.1 h, and 12.5 h at pH 5.0, pH 7.0, and pH 12.0, respectively, indicating that carthamin is much more stable at alkaline pH than acidic or neutral conditions. The activation energies of carthamin at pH 5.0, pH 7.0, and pH 12.0 were 15.6, 15.7 and 16.8 kcal/mol, respectively.

• ALGAE
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• v.21 no.3
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• pp.317-321
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• 2006

The effects pH and salinity on the brown alga Sargassum fulvellum were investigated in the context of the removal of two major associated animals, Caprella scaura and Gammaropsis utinomi. Optimum quantum yield (Fv/Fm) of S. fulvellum was also examined in the same experimental conditions as an index of stress. Experiments on pH and salinity tolerances of the two animal species indicated that mortality was more than 80% at extremes of pH (2, 3, 4, 10, 11, 12) and salinity (0, 3.5, 7, 10, 44 psu) after a 5 min treatment. Lethal time (LT50) from pH 2 to pH 4 was less than 90 sec in C. scaura, and less than 70 sec in G. utinomi. From 0 to 10 psu, LT50 was less than 20 sec in C. scaura and less than 60 sec in G. utinomi. The quantum yield of S. fulvellum was not significantly different from controls within the pH range 4~10, and within the salinity range of 7-40 psu. When exposed to pH and salinity conditions outside these ranges, the effect of these factors on the removal of two animal species was higher, but quantum yield was highly reduced. These results indicate that the optimal conditions for removing the animal species without affecting optimum quantum yield were pH 4-10, and salinities 7-10 psu and 44 psu.

• Korean Journal of Food Science and Technology
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• v.22 no.1
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• pp.56-60
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• 1990

The changes in the partition coefficient of model proteins (lysozyme, myoglobin, conalbumin, bovine serum albumin) in an aqueous two-phase system formed by polyethylene glycol and dextran were examined in order to improve the capacity of counter current distribution for the protein fractionation and concentration. The protein distribution patterns in CCD with 30 tubes varied with the pH of the system, and both theoretical and measured values agreed well. From the mixture of model protein, pure BSA fraction was appeared at the upper-phase of 14th tube having pH 4.5, pure myoglobin at the lower-phase of the 16th be with pH 6.5 and conalbumin at the lower-phase of 4th tube with pH 12. The result indicated the possible use of CCD method for protein fractionation, if the partition coefficient of proteins was manipulated by pH and other means.

• Biomedical Science Letters
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• v.29 no.4
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• pp.287-295
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• 2023

Amifostine was developed to protect cells, but it is known to induce cytotoxicity and apoptosis, and the exact mechanism is unknown. In this study, we investigated how the DNA mismatch repair (MMR) system interacts with p53 to prevent apoptosis, cell cycle arrest, and cytoprotective effects induced by amifostine. HCT116 colon cancer cells sublines HCT116/p53+,HCT116/p53+, HCT116/p53-, HCT116/E6 and HCT116+ch3/E6 cells were used for evaluation. Amifostine induced G1 arrest and increased toxicity two-fold in p53- cells regardless of MMR expression. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Both G1 cell cycle arrest and induction of p53 protein peaked at 24 h after the start of amifostine exposure. Amifostine induced the expression of p21 protein in both p53+ and p53- cells. As for apoptosis, compared to p53- cells, p53+ cells showed 3.5~4.2 times resistance to amifostine-induced apoptosis. HCT116+E6 with both p53 and MMR loss showed maximum apoptosis at 48 h, and HCT116+ch3/E6HCT116+ch3/E6 with p53 loss showed maximum apoptosis at 24 h. As a result, it was confirmed through in vitro experiments that amifostine-induced G1 cell cycle arrest and apoptosis are mediated through a pathway dependent on MMR and p53 protein.

• Proceedings of the KSRS Conference
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• 1998.09a
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• pp.382-386
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• 1998

The objectives of this study are to reveal relationship between tree physiology and spectral reflectance on effects of artificial acid rain and to obtain basic data on optimal wave length for forest of LRC sensor on KOMPSAT-2. Three pH levels of artificial acid rain - control, pH4.5 and pH3.0 - were applied to Pinus and Quercus species. Three types of the acid rain were spraied at the amount of 500m1 in every two days. Spectral reflectance data was collected once in a month by using GER 1500 (350~2500nm) or Ll 1800(300~1100nm) Spectroradiometer. The data was measured three times in a pH level. The results of this study are as follows; in April, the spectral reflectance of Pinus species was high in order at the level of pH3.0, control and pH4.5; in May, control, pH3.0 and pH4.5; in June, control, pH4.5 and pH3.0. That of Quercus species was high in the order of control, pH4.5 and pH3.0 in May; in June, control, pH3.0 and pH4.5, especially, within infrared wave length range, control, pH4.5 and ph3.0.

• Analytical Science and Technology
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• v.7 no.1
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• pp.25-31
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• 1994

As for analytes which did not represent the differences of the selectivity on the stationary and mobile phase, secondary chemical equilibrium theory was applied to study pH effects on the separation of alkylphenols. Mobile phase was consisted of an aqueous sodium carbonate-bicarbonate buffer and acetonitrile. The maximum selectivity for adjacent peak pairs was predicted from those values of $k^{\prime}_{HA}/k^{\prime}_{A-}$ and ${\Delta}pK$. The optimum pH determined by this method was 11.18 pH and solvent selectivity were considered at the same time to invoke the full range of selectivity effects possible for separations. Quaternary mixture composed of methanol, acetonitrile, tetrahydrofuran and water was adjusted to optimum pH 11.18. As the statistical simplex technique of an overlapping resolution map (ORM) was used to predict the optimized solvent system. The optimum solvent, which gives complete separation of alkylphenols, was determined as follws MeOH : ACN : THF = 14.4 : 81.8 : 2.8.

• Korean Journal of Poultry Science
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• v.24 no.4
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• pp.199-206
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• 1997

In this experiment, three kinds of autoclaved poultry by-product meals, P1 (head+feet+viscera), P2(P1+blood), and P3(P2+feathers), mixed as to the yielding ratios, and three kinds of autoclaved hatchery by-products meals, H1 (male d-old chicks of egg strain), H2(dead embryos ＆ infertile eggs), and H3(H1+H2, 2:1) were formulated into the layer diets each at 5％ level, respectively, and compared with the control diets(C) containing fishmeal on laying performances and egg qualifies. The egg production, average egg weight, feed intake and feed conversion were not significantly affected by the poultry or hatchery by-product meals. The egg shell qualities, such as the egg specific gravity, egg breaking strength and shell thickness, were not different among treatments. The egg yolk colour was improved by the feeding of hatchery by-products meals (P<0.05). In conclusion, pultry and hatchery by-product meals could be used in layer diets without any adverse effects on laying performances to substitute for fishmeal at about 5％ level.